RESUMO
OBJECTIVES: This study investigates the effect of stellate ganglion (SG) phototherapy in healthy participants and assesses its efficacy in suppressing electrical storm (ES) refractory to antiarrhythmic drugs and catheter ablation. BACKGROUND: Modulation of the autonomic nervous system has been shown to be an effective adjunctive therapy for ES. METHODS: Ten-minute SG phototherapy was performed twice weekly for 4 weeks in 20 healthy volunteers. To evaluate the acute and chronic effects of SG phototherapy, heart rate variability and serum concentrations of adrenaline, noradrenaline, and dopamine were obtained before phototherapy, immediately after the first phototherapy session, after 8 sessions of phototherapy, and 3 months after the first phototherapy session. In addition, the efficacy of SG phototherapy was evaluated in 11 patients with ES refractory to medication, sedation, and catheter ablation. RESULTS: In healthy participants, serum adrenaline concentration significantly decreased after phototherapy, whereas low-frequency power/high-frequency power significantly decreased during phototherapy. Moreover, the effect on heart rate variability did not last beyond 3 months. In the clinical pilot study, 7 patients had a suppression of ES after SG phototherapy; however, without maintenance therapy, 2 patients had a recurrence of ventricular arrhythmias. Furthermore, it did not control ES in 4 patients. CONCLUSIONS: SG phototherapy reduced sympathetic activity and may be a safe and effective adjunctive therapy to control ES in some patients, but its long-term efficacy remains unknown. Chronic phototherapy might help reduce ES recurrence.
Assuntos
Gânglio Estrelado , Taquicardia Ventricular , Arritmias Cardíacas , Humanos , Lasers , Fototerapia , Projetos PilotoRESUMO
AIMS: The His bundle electrogram is believed to reflect the exact location of the His bundle. However, the distinction between distal His bundle potential and proximal right bundle branch potential is challenging. The aim of this study was to pinpoint the location of the branching point of the His bundle, and to compare that site with the site of recording of the largest His bundle electrogram (LH) during sinus rhythm. METHODS: We hypothesized that the site of earliest His activation (EH) during retrograde conduction via the left bundle branch is the branching point. We studied 15 nonconsecutive patients (mean age = 40 +/- 22 years; eight men). We performed a programmed stimulation from right ventricular apex until retrograde right bundle branch block appeared. At that point we measured (1) the distance between antegrade LH site and retrograde EH site and (2) the atrial-to-ventricular amplitude ratio (A/V ratio) at both sites. RESULTS: EH was recorded at the proximal electrode of the His bundle catheter in all patients. Mean distance between EH and LH was 9.8 +/- 2.5 mm. The mean A/V ratios at the EH site and the LH site were 1.01 +/- 0.42 and 0.08 +/- 0.06, respectively. DISCUSSION: This study showed that the EH site is located approximately 10-mm proximal to the LH site. The mean A/V ratio at the EH site during sinus rhythm is approximately 1.0. These observations suggest that the majority of His potentials reflect proximal right bundle activation. Before delivering radiofrequency energy in the para-Hisian area, attention should be paid to the presence of a His potential and to the A/V ratio, rather to the amplitude of the His electrogram.
Assuntos
Mapeamento Potencial de Superfície Corporal/métodos , Fascículo Atrioventricular/fisiopatologia , Bloqueio de Ramo/diagnóstico , Bloqueio de Ramo/fisiopatologia , Técnicas Eletrofisiológicas Cardíacas/métodos , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
AIMS: To compare the risk of atrioventricular (AV) conduction disturbance between patients with sinus node dysfunction on AAI pacing who had a low or high Wenckebach block rate (WBR). METHODS AND RESULTS: Patients with sinus node dysfunction and normal AV conduction those underwent an electrophysiological study were studied. The patients were classified into two groups: Group L was with the patients with a WBR of 100 to 129 per minute and Group H was with the patients with a WBR > or = 130 per minute. All patients followed up every 3-6 months after an AAI pacemaker implantation. A total of 102 patients, including 35 Group L and 67 Group H, were followed for 90 +/- 44 months. Six patients died from non-cardiac cause and five patients required a new atrial lead implantation due to lead failure during follow-up. Symptomatic bradycardia requiring a new ventricular lead implantation developed in four patients (annual incidence 0.5%). In Group L, two patients developed AV block (annual incidence 0.7%). In Group H, two patients developed bradycardic atrial fibrillation (annual incidence 0.4%). Kaplan-Meier analysis revealed no significant difference between the two groups (P = 0.2983). CONCLUSION: These results suggest that a long-term risk of developing AV conduction disturbance is low even in patients with a WBR of 100 to 129 per minute.
Assuntos
Bloqueio Atrioventricular/etiologia , Bloqueio Atrioventricular/terapia , Estimulação Cardíaca Artificial/métodos , Marca-Passo Artificial , Síndrome do Nó Sinusal/terapia , Idoso , Idoso de 80 Anos ou mais , Bradicardia/etiologia , Estimulação Cardíaca Artificial/efeitos adversos , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Fatores de Risco , Síndrome do Nó Sinusal/complicações , Fatores de Tempo , Resultado do TratamentoRESUMO
We present two cases of common-type atrial flutter with a large bystander segment without double potentials in the cavotricuspid isthmus. In both cases, right atrial angiography demonstrated a prominent pouch at the center of the isthmus. When radiofrequency energy was applied to the tricuspid side of the isthmus, delayed potentials abruptly appeared on the local electrograms. When radiofrequency energy was applied on the inferior vena cava side of the isthmus, the tachycardia was terminated. Although ablation was not applied to the bottom of the pouch, bidirectional isthmus block was achieved. These outcomes indicate that the pouch represented a bystander segment.
Assuntos
Flutter Atrial/fisiopatologia , Efeito Espectador/fisiologia , Cardiomiopatias/complicações , Eletrocardiografia , Processamento de Sinais Assistido por Computador , Valva Tricúspide/fisiopatologia , Veia Cava Inferior/fisiopatologia , Idoso , Idoso de 80 Anos ou mais , Amiloidose/complicações , Amiloidose/fisiopatologia , Flutter Atrial/cirurgia , Estimulação Cardíaca Artificial , Cardiomiopatias/fisiopatologia , Ablação por Cateter , Feminino , Átrios do Coração/cirurgia , Insuficiência Cardíaca/complicações , Insuficiência Cardíaca/fisiopatologia , Neoplasias Cardíacas/cirurgia , Humanos , Masculino , Mixoma/cirurgia , Complicações Pós-Operatórias/fisiopatologia , Complicações Pós-Operatórias/cirurgia , Taquicardia/fisiopatologia , Taquicardia/cirurgia , Veia Cava Inferior/cirurgiaRESUMO
A 76-year-old man with two different sustained atrial arrhythmias that occurred after coronary artery bypass grafting underwent electrophysiological studies. Macroreentrant atrial tachycardias were detected with an isolated slow pathway mimicking focal activation on three-dimensional electroanatomical mapping. The slow conduction pathway in the right atrial free wall was assumed to represent tissue damaged by right atrial cannulation during previous coronary artery bypass grafting.
Assuntos
Mapeamento Potencial de Superfície Corporal/métodos , Ponte de Artéria Coronária/efeitos adversos , Sistema de Condução Cardíaco/lesões , Sistema de Condução Cardíaco/cirurgia , Imageamento Tridimensional/métodos , Taquicardia por Reentrada no Nó Atrioventricular/diagnóstico , Taquicardia por Reentrada no Nó Atrioventricular/cirurgia , Idoso , Ablação por Cateter , Diagnóstico Diferencial , Humanos , Masculino , Taquicardia por Reentrada no Nó Atrioventricular/etiologia , Resultado do TratamentoRESUMO
A 36-year-old woman presented with drug-refractory atrial tachycardia. During the tachycardia episodes, P waves were positive in leads II, III, aVF, and V1, while they were negative in leads I and aVL. It was hard to determine whether the origin was the left atrial appendage or left superior pulmonary vein on the surface electrocardiogram. Electrophysiologic evaluation revealed that the earliest endocardial activation occurred at the base of the left atrial appendage, preceding the onset of P waves by 38 ms. On initiation of the tachycardia, a warm-up phenomenon was observed. There was a fixed relation between the coupling interval of a single extrastimulus and the return cycle length during the tachycardia. These findings suggested that the mechanism of the tachycardia was automaticity. Application of radiofrequency energy at the left atrial appendage terminated the tachycardia and it was not inducible after ablation.
Assuntos
Apêndice Atrial/cirurgia , Ablação por Cateter , Taquicardia Atrial Ectópica/cirurgia , Adulto , Apêndice Atrial/fisiopatologia , Feminino , Sistema de Condução Cardíaco/fisiopatologia , Sistema de Condução Cardíaco/cirurgia , Humanos , Taquicardia Atrial Ectópica/fisiopatologiaRESUMO
BACKGROUND: The inferior vena cava (IVC) is obliquely connected to the right atrium (RA), and often a low-voltage area is observed in the posteroinferior RA. OBJECTIVES: The purpose of this study was investigate the size of the IVC extension into RA and its anatomic background. METHODS: We investigated 30 human hearts [11 men and 19 women; mean age 79 +/- 10 years; 7 cardiac deaths (group A) and 23 noncardiac deaths (group B)]. After obtaining macroscopic measurements around the RA-IVC junction, serial sections were cut and examined histologically. We defined a horizontal baseline at the level of the cavotricuspid isthmus and measured (1) the length of IVC extension, which was defined as the distance from the baseline to the top of the RA-IVC junction, and (2) the width of the RA-IVC junction at the baseline level. RESULTS: The top of the RA-IVC junction was always located in the posteroinferior RA. The mean length of the IVC extension was 17.6 +/- 6.6 mm, and the mean width was 29.6 +/- 7.5 mm. The IVC extension was wider in group A than in group B (35.8 +/- 9.0 mm vs 27.5 +/- 5.9 mm; P = .0277). The right and left borders of the RA-IVC junction corresponded to the reflection of the pericardium. Histologic examination showed no myocardium in the IVC extension. CONCLUSION: The IVC extension into the posteroinferior RA always exists and varies in size. Because this area lacks myocardium, it is important to consider when analyzing catheter mapping from this area.
Assuntos
Coração/anatomia & histologia , Miocárdio/citologia , Veia Cava Inferior/anatomia & histologia , Idoso , Idoso de 80 Anos ou mais , Morte , Eletrocardiografia , Feminino , Átrios do Coração/anatomia & histologia , Átrios do Coração/citologia , Humanos , Masculino , Pessoa de Meia-Idade , Valores de ReferênciaRESUMO
A 68-year-old man died a few days after catheter ablation of drug-resistant, monomorphic ventricular tachycardia (VT) complicated with cardiac sarcoidosis. The diagnosis of mitral isthmus VT was made from electrophysiological observations, including electro-anatomical activation and voltage map, pace-mapping, entrainment mapping and ablation outcome. On autopsy of the heart, sarcoidic lesion with scattered fibrous tissue in the mitral isthmus was non-transmural, and the surviving myocardium serving as the reentry circuit in the endomyocardium was isolated from the adjacent viable epimyocardium, enabling the sustenance of macroreentry across the mitral isthmus. Non-transmural lesions produced by RF delivery created a barrier sufficient to interrupt the myocardial bundles located in the mitral isthmus, eliminating the mitral isthmus VT.
Assuntos
Cardiomiopatias/patologia , Ablação por Cateter , Valva Mitral/patologia , Sarcoidose/patologia , Taquicardia Ventricular/patologia , Idoso , Cardiomiopatias/complicações , Ablação por Cateter/métodos , Evolução Fatal , Humanos , Masculino , Sarcoidose/complicações , Taquicardia Ventricular/complicaçõesRESUMO
It is unknown whether salicylate enhances the action of antiarrhythmic agents on human Na+ channels with state dependency and tissue specificity. We therefore investigated effects of salicylate on quinidine-induced block of human cardiac and skeletal muscle Na+ channels. Human cardiac wild-type (hH1), LQT3-related mutant (ΔKPQ), and skeletal muscle (hSkM1) Na+ channel α subunits were expressed in COS7 cells. Effects of salicylate on quinidine-induced tonic and use-dependent block of Na+ channel currents were examined by the whole-cell patch-clamp technique. Salicylate enhanced the quinidine-induced tonic and use-dependent block of both hH1 and hSkM1 currents at a holding potential (HP) of -100 mV but not at -140 mV. Salicylate decreased the IC50 value for the quinidine-induced tonic block of hH1 at an HP of -100 mV, and produced a negative shift in the steady-state inactivation curve of hH1 in the presence of quinidine. According to the modulated receptor theory, it is probable that salicylate decreases the dissociation constant for quinidine binding to inactivated-state channels. Furthermore, salicylate significantly enhanced the quinidine-induced tonic and use-dependent block of the peak and steady-state ΔKPQ channel currents. The results suggest that salicylate enhances quinidine-induced block of Na+ channels via increasing the affinity of quinidine to inactivated state channels.
Assuntos
Quinidina/farmacologia , Salicilatos/farmacologia , Bloqueadores dos Canais de Sódio/farmacologia , Canais de Sódio/genética , Canais de Sódio/metabolismo , Animais , Células COS , Chlorocebus aethiops , Coração/efeitos dos fármacos , Humanos , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/genética , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Mutação , Miocárdio/metabolismo , Canal de Sódio Disparado por Voltagem NAV1.5 , Ligação Proteica , Quinidina/metabolismoRESUMO
We examined autopsy specimens from a patient with ischemic cardiomyopathy who underwent radiofrequency catheter ablation of ventricular fibrillation. There was site specific arrhythmogenesis of the trigger ventricular premature contractions (VPCs) and Purkinje potentials were recorded before the onset of the QRS. In postmortem examination, fibromuscular bands connecting the posterior papillary muscle and ventricular septum were recognized at the successful ablation sites of the trigger VPCs and the microscopic examinations revealed Purkinje cells in the center of that fibromuscular band.
Assuntos
Cardiomiopatias/patologia , Cardiomiopatias/cirurgia , Sistema de Condução Cardíaco/patologia , Sistema de Condução Cardíaco/cirurgia , Isquemia Miocárdica/patologia , Isquemia Miocárdica/cirurgia , Fibrilação Ventricular/patologia , Fibrilação Ventricular/cirurgia , Cardiomiopatias/complicações , Humanos , Masculino , Pessoa de Meia-Idade , Isquemia Miocárdica/complicações , Fibrilação Ventricular/complicaçõesRESUMO
BACKGROUND: Coronary artery damage has been reported during catheter ablation procedures. Recently, linear ablation of thin left atrial tissue has been performed for atrial fibrillation. OBJECTIVE AND METHODS: Because we have little information about the arteries in the left atrium, this study was performed to evaluate the anatomy of these arteries, and to compare them with previously reported ablation lines. Coronary angiography was performed in 262 patients. Atrial coronary arteries between the left atrial appendage and the left superior pulmonary vein (LAA-LSPV region), as well as between the left inferior pulmonary vein and the mitral annulus ("mitral isthmus" region) were examined. RESULTS: Atrial coronary arteries extending to the LAA-LSPV region were found in 92 subjects (35%), while arteries crossing the mitral isthmus region were found in 119 subjects (46%). Atrial coronary arteries crossed the ablation line in about 69% of subjects overall. CONCLUSION: These results might suggest a risk of acute complications due to left atrial ablation. Alternatively, recurrence of atrial fibrillation might be caused by protected myocardium around the atrial arteries. We should note that atrial coronary arteries cross the ablation line in many patients.
Assuntos
Fibrilação Atrial/diagnóstico por imagem , Fibrilação Atrial/cirurgia , Ablação por Cateter/métodos , Angiografia Coronária , Vasos Coronários/cirurgia , Átrios do Coração/diagnóstico por imagem , Átrios do Coração/cirurgia , Idoso , Feminino , Humanos , MasculinoRESUMO
During para-Hisian pacing, widening of the paced QRS complex usually indicates loss of His bundle capture. We describe a patient without any accessory pathways in whom widening of the paced QRS complex occurred due to loss of left bundle branch capture during para-Hisian pacing. After initial widening of the QRS complex, further widening was observed due to loss of His bundle capture. With the initial QRS widening, the stimulus-atrial interval and retrograde atrial activation sequence were almost unchanged, so the findings mimicked retrograde conduction over an accessory pathway. This may be a pitfall of the para-Hisian pacing technique.
Assuntos
Bloqueio de Ramo/diagnóstico , Bloqueio de Ramo/prevenção & controle , Estimulação Cardíaca Artificial/métodos , Eletrocardiografia/métodos , Taquicardia Ventricular/diagnóstico , Taquicardia Ventricular/prevenção & controle , Adulto , Diagnóstico Diferencial , Feminino , HumanosRESUMO
Annelids as a group express a variety of phosphagen kinases including creatine kinase (CK), glyocyamine kinase (GK), lombricine kinase (LK), taurocyamine kinase (TK) and a unique arginine kinase (AK) restricted to annelids. In prior work, we have determined and compared the intron/exon organization of the annelid genes for cytoplasmic GK, LK, AK, and mitochondrial TK and LK (MiTK and MiLK, respectively), and found that these annelid genes, irrespective of cytoplasmic or mitochondrial, have the same 8-intron/9-exon organization strikingly similar to mitochondrial CK (MiCK) genes. These results support the view that the MiCK gene is basal and ancestral to the phosphagen kinases unique to annelids. To gain a greater understanding of the evolutionary processes leading to the diversity of annelid phosphagen kinases, we determined for the first time the intron/exon organization of a cytoplasmic CK gene from a polychaete as well as that of another polychaete MiCK gene. These gene structures, coupled with a phylogenetic analyses of annelid enzymes and assessment of the fidelity of substrate specificity of some these phosphagen kinases, provide insight into the pattern of radiation of the annelid enzymes. Annelid phosphagen kinases appeared to have diverged in the following order (earliest first): (1) cytoplasmic AK, LK and TK, (2) GK, and (3) mitochondrial MiLK and MiTK. Interestingly, phylogenetic analyses showed that the above phosphagen kinases appear to be basal to all CK isoforms (mitochondrial, cytoplasmic and flagellar CKs). This somewhat paradoxical placement of CKs most likely reflects a higher rate of evolution and radiation of the annelid-specific LK, TK and GK genes than the CK isoform genes.
Assuntos
Anelídeos/enzimologia , Creatina Quinase/genética , Evolução Molecular , Animais , Citoplasma/enzimologia , Éxons , Íntrons , Proteínas Mitocondriais , Fosfotransferases (Aceptor do Grupo Nitrogenado)/genética , Isoformas de Proteínas/genética , Especificidade por SubstratoRESUMO
Previously, complications associated with the placement of the left ventricular pacing lead were reported in 1.9-6% of cases. We describe a case with a stripping of venous intima from the coronary sinus by a guidewire during a left ventricular lead implantation. Judging from this case, the firm guidewire and coronary catheter should not be used within the coronary sinus.
Assuntos
Estimulação Cardíaca Artificial , Seio Coronário/cirurgia , Túnica Íntima/cirurgia , Idoso , Humanos , MasculinoRESUMO
We report a novel action of intracellular adenosine monophosphate (AMP) to inhibit beta-adrenergic signaling in isolated rat ventricular myocytes. Extracellular application of adenosine or AMP suppressed isoproterenol (Iso)-induced prolongation of action potential duration (APD). This effect was completely abolished by an A(1)-receptor antagonist, DPCPX. Intracellular application of AMP, but not adenosine, attenuated Iso-induced APD prolongation. Iso-induced increases in the L-type Ca(2+) current (I(Ca,L)) were also inhibited by intracellular AMP. These inhibitory effects were not affected by either DPCPX or glibenclamide. In vitro, AMP directly inhibited PKA activity via binding to its regulatory subunit. These results suggest that intracellular AMP attenuates beta-adrenergic signaling by directly inhibiting PKA activity, independently of A(1)-purinergic receptor.
Assuntos
Monofosfato de Adenosina/farmacologia , Membrana Celular/fisiologia , Coração/fisiologia , Receptores Adrenérgicos beta/fisiologia , Receptores Purinérgicos P1/fisiologia , Potenciais de Ação/efeitos dos fármacos , Adenosina/farmacologia , Animais , Membrana Celular/efeitos dos fármacos , Ventrículos do Coração/efeitos dos fármacos , Ratos , Ratos Wistar , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia , Função VentricularRESUMO
Histopathologic examination of the cavotricuspid isthmus in which a large-tip catheter was necessary to achieve conduction block is presented. No thickened myocardium or prominent trabeculation was observed on the ablation line. A small cardiac vein extending through the isthmus across the ablation scar was detected. The remaining myocardial cells were distributed along the small cardiac vein. It is possible that the luminal blood flow of the small cardiac vein protects the surrounding atrial muscle from effective delivery of radiofrequency energy.