Motor resonance to non-visible postural adaptation: A novel aspect of the mirror mechanism.

The activation of the Mirror Neuron System (MNS) has been described to reflect visible movements, but not postural, non-visible, adaptations that accompany the observed movements. Since any motor act is the result of a well-tailored dialogue between these two components, we decided to investigate whether a motor resonance to nonvisible postural adaptations could be detected. Possible changes in soleus corticospinal excitability were investigated by eliciting the H-reflex during the observation of three videos, corresponding to three distinct experimental conditions: 'Chest pass', 'Standing' and 'Sitting', and comparing its size with that measured during observation of a control videoclip (a landscape). In the observed experimental conditions, the Soleus muscle has different postural roles: a dynamic role in postural adaptations during the Chest pass; a static role while Standing still; no role while Sitting. The H-reflex amplitude was significantly enhanced in the 'Chest pass' condition compared to the 'Sitting' and 'Standing' conditions. No significant difference was found between 'Sitting' and 'Standing' conditions. The increased corticospinal excitability of the Soleus during the 'Chest pass' condition suggests that the mirror mechanisms produce a resonance to postural components of an observed action, although they may not be visible. This observation highlights the fact that mirror mechanisms echo non intentional movements as well and points to a novel possible role of mirror neurons in motor recovery.

Hair cortisol reduction and social integration enhancement after a mindfulness-based intervention in children.

BACKGROUND: Over recent decades, chronic stress at an early age has become a worrying health problem in children. We seek to evaluate an intervention involving mindfulness-based practices and prosocial activities in 7- to 8-year-old children. METHODS: Stress levels were determined using hair cortisol concentration (HCC), and social integration was measured by means of a sociogram. The program had previously proven to be effective in decreasing salivary cortisol levels and in favouring social integration in children. A total of 35 children participated in the study: 18 constituted the intervention group and 17 the wait-list group. In both groups, HCC and social integration were evaluated before and after the intervention conducted throughout an entire school year. RESULTS: The experimental group showed a significant reduction in HCC, as well as significant enhancement of social integration levels, whereas no changes were observed in the wait-list group. CONCLUSIONS: This is the first research to show that HCC, a reliable neuroendocrine indicator, decreased as a result of a mindfulness-based program. This successful outcome adds new evidence to previous findings regarding the reduction of chronic stress in children following participation in this program.

How to make the Lunar and Martian soils suitable for food production - Assessing the changes after manure addition and implications for plant growth.

The in-situ resource utilisation (ISRU), in terms of native rocky materials and astronaut wastes, is crucial in contests of soil-based space-farming. Nevertheless, extra-terrestrial soils are very different from Earth soils, lacking any form of organic carbon and associated macro and micronutrients. In this research, we aimed to study and modify two commercially available Lunar and Martian regolith simulants (LHS-1 from Exolith Lab and MMS-1 from Martian Garden) to make them an adequate medium for plant growth. Lettuce was chosen as reference crop to guide the discussion on the results obtained. To reach this main objective, we added to simulants a commercially available monogastric-based organic manure chosen as a substitute of a possible organic amendment produced onboard. The simulant/manure mixture rates were 100:0, 90:10, 70:30, 50:50; w:w. As expected, an approximately linear increase of total and bioavailable contents of macro (N, S, P, Ca, K, Mg) and micro (Fe, Mn, Cu, Zn) nutrients with increasing manure addition to simulants was observed. On the other hand, the very high pH of manure (pH, 9.02) along with its salinity (EC, 6.7 dS m-1) and sodicity (Na, 5.3 g kg-1), did not correct the already high pH of simulants (very high for LHS-1), but rather raised their soluble salt content and sodium amount on the exchange complex. In addition, an increase of toxic soluble aluminium and heavy elements (Pb, Ni, Cr, V) was observed, mainly in the strongly alkaline lunar simulant/manure mixtures. The addition of an organic source also produced a generalised improvement of water retention and hydraulic conductivity of both regolith simulants, in proportion to the percentage of manure addiction. For both situations, the best mixture ratio was 70:30. In terms of water retained, the LHS-1 mixtures benefited more than the MMS-1 ones by manure addition since water was held more in the "dry" (between -100 and -600 cm of matric potential head) than in the "humid" (between -25 and -100 cm of matric potential head) region of water retention. This would make LHS-1 mixtures more useful for cultivation of lettuce, at least in terms of physico-hydraulic properties. Nevertheless, the overall characterisation of the mixtures unveiled that MMS-1-based substrates can ensure better agronomic performances than LHS-1 ones, mainly due to lower pHs and higher nutrient availability; this divergent fertility was particularly evident at 90:10 simulant/manure rate and tend to be mitigated by increasing the levels of manure.

Compost and microbial biostimulant applications improve plant growth and soil biological fertility of a grass-based phytostabilization system.

In this work, a grass-based phytoremediation system integrated with an organic amendment and biostimulants was evaluated for remediating contaminated sites. Plant growth and biological fertility were monitored to assess the efficacy of a vegetative cap used as a safety measure to reduce sanitary and environmental risks of industrially contaminated soils and soil-washing sludges. Both matrices were potentially contaminated with Pb and Zn with an ecological risk index from low to moderate. According to potentially toxic elements (PTEs) bioaccessibility tests, the exposure to the released fine particulate matter may cause serious risks to human beings, in particular to children. The grass mixture was well adapted to both the substrates and a low PTEs mobility was detected, thus, reducing the leaching risk to ground water sources. Compost addition augmented significantly nitrogenase reductase (nifH) and ammonia monooxygenase (amoA) gene expression abundance in both substrates. Furthermore, a positive interaction between compost fertilization and a Trichoderma-based biostimulant inoculation was recorded in sludges resulting in a significant stimulation of nitrogen-fixing and ammonia-oxidizing bacteria. The application of compost and biostimulant increased soil fertility and plant growth. Furthermore, there was a slight reduction in PTE bioaccessibility, thus, improving the efficiency of the phytostabilization, limiting the resuspension and dispersion of the health-risk soil particulate.

Reliability of Shoulder Helical Axes During Intransitive and Transitive Upper Limb Tasks.

Shoulder complex stability can be estimated in vivo through the analysis of helical axes (HAs) dispersion during upper limb movements. The study aimed at investigating test-retest reliability of shoulder HAs dispersion parameters during upper limb tasks. Twenty healthy volunteers performed 2 intransitive (shoulder flexion and rotation) and one transitive (combing) tasks with the dominant and nondominant upper limbs during 2 recording sessions at 1-week distance. Kinematics was detected through an optoelectronic system. Mean distance and mean angle (MA) were adopted as HAs dispersion indexes. Reliability was excellent for mean distance (intraclass correlation coefficient [ICC]: .91) and MA (ICC: .92) during dominant flexion, and good for MA (ICC: .90) during nondominant flexion. Moderate reliability was found for HAs parameters during rotation (ICCs from .70 to .59), except for MA during dominant rotation where reliability was poor. Reliability was good for mean distance (ICC: .83) and moderate for MA (ICC: .67) during the dominant combing task, whereas no reliability was found during the nondominant combing task. HAs dispersion parameters revealed high reliability during simple intransitive tasks with the dominant limb. Reliability decreased with the increase in task complexity due to the increase in movement variability. HAs dispersion technique could be used to assess shoulder complex stability in patients after rehabilitation or surgery.

Heavy metal load and effects on biochemical properties in urban soils of a medium-sized city, Ancona, Italy.

Urban soils are often mixed with extraneous materials and show a high spatial variability that determine great differences from their agricultural or natural counterparts. The soils of 18 localities of a medium-sized city (Ancona, Italy) were analysed for their main physicochemical and biological properties, and for chromium (Cr), copper (Cu), cobalt (Co), lead (Pb), nickel (Ni), zinc (Zn), and mercury (Hg) total content, distribution among particle-size fractions, and extractability. Because of the absence of thresholds defining a hot spot for heavy metal pollution in urban soils, we defined a "threshold of attention" (ToA) for each heavy metal aiming to bring out hot spot soils where it is more impellent to intervene to mitigate or avoid potential environmental concerns. In several city locations, the soil displayed sub-alkaline pH, large contents of clay-size particles, and higher TOC, total N, and available P with respect to the surrounding rural areas, joined with high contents of total heavy metals, but low availability. The C biomass, basal respiration, qCO2, and enzyme activities were compared to that detected in the near rural soils, and results suggested that heavy metals content has not substantially compromised the soil ecological services. We conclude that ToA can be considered as a valuable tool to highlight soil hot spots especially for cities with a long material history and, for a proper risk assessment in urban soils, we suggest considering the content of available heavy metals (rather than the total content) and soil functions.

Bioaccessibility of potentially toxic metals in soil, sediments and tailings from a north Africa phosphate-mining area: Insight into human health risk assessment.

The risk assessment of phosphate mining/processing industrial activities on the environment and human health is crucial to properly manage and minimize the risks over time. In this work, we studied the inhalation and dermal bioaccessibility of potentially toxic metals (PTM) in different particle-size fractions of urban soil, sediments and tailings from Gafsa-Metlaoui phosphate mining area, to assess afterwards the non-carcinogenic (NCR) and carcinogenic (CR) risks for the health of local citizens and workers constantly exposed to airborne particulate matter (PM) originating from these sources of contamination. Samples were separated in particle-size fractions by centrifugation and consecutive cycles of sedimentation and decanting. The pseudo-total concentrations and bioaccessible fractions of PTM were extracted by aqua regia and in vitro bioaccessibility tests, respectively. Both sediments and tailings showed higher-than-background concentrations of PTM (mainly Cd, Zn and Cr), with a tendency to accumulate these metals in fine particles (<10 µm). In urban soil, only Cd was above the background concentration. The bioaccessibility of PTM via inhalation was significantly higher in artificial lysosomal fluid (ALF) than in simulated epithelial lung fluid (SELF): basically, Cd was the most bioaccessible metal (relative bioaccessibility up to 80%), followed by the medium-to-high bioaccessible Zn (47%), Pb (46%) and Cu (39%), and the least bioaccessible Cr (16%). In synthetic skin surface liquid (NIHS 96-10), only Cd was bioaccessible at worrying extent (20-44%). On the basis of US.EPA risk assessment, the exposure to PTM bioaccessible fractions or pseudo-total concentrations would not cause serious NCR and CR risks for human health. Significant health risks (Hazard Index >1 and CR > 10-4), especially for children, can occur if ingestion route is also considered. The findings underline the need for adequate protection of contaminated soil, sediments and mine tailings laying nearby urban agglomerates, to reduce the health risks for inhabitants and workers of Gafsa-Metlaoui mining area.

Environmental and human health risk assessment of potentially toxic elements in soil, sediments, and ore-processing wastes from a mining area of southwestern Tunisia.

The occurrence and bioaccessibility of potentially toxic elements (PTEs) in soils and sediments are investigated by many studies, especially in territories exploited by mining and ore-processing activities, nearby agriculture-driven rural cities. Accordingly, the present study aimed at evaluating the geochemical properties, potential bioavailability, and risks for environment and human health of the most concerning PTEs of study area (Gafsa mining basin, Tunisia) such as Cd, Cr, and Zn in selected soil, sediment, and mining waste samples. The extraction of these solid matrixes by modified EU-BCR sequential extraction revealed that the most easily extractable fractions of each PTE were very low (first 2 steps, < 10%), Cd was mainly associated with the oxidizable phase (likely organic matter), and Cr and Zn were mostly found in residual mineral fraction (likely occluded in non-siliceous mineral phase). The total cumulative concentration of each metal was found to be higher in soil/sediment profiles and ore-processing wastes than in phosphate rocks, indicating a metal enrichment due to mining activities. The aqua regia extraction of representative sediment samples revealed that Cd, Cr, and Zn concentrations were higher than non-polluted sediment standards. In contrast, other elements as Cu, Mn, and Pb essentially arose from natural bedrocks. The Unified BARGE method was applied to assess the risk of ingestion by human beings and wild/domestic animals of contaminated sediment particulate prone to wind erosion and air dispersion in the arid conditions of study area. An higher oral bioaccessibility was found for Cd than Zn and Cr, most concerning in acid gastric phase than in sub-neutral intestinal environment.

Background element content in the lichen Pseudevernia furfuracea: a comparative analysis of digestion methods.

In bioaccumulation studies, the interpretation of pollutant contents in the target biomonitor has to be performed by assessing a deviation from an unaltered reference condition. A common strategy consists in the comparison with background element content (BEC) values, often built up by uncritically merging methodologically heterogeneous data. In this respect, the acid digestion of samples was identified as a major step affecting BEC data. Here, the analytical outcomes of two acid mixtures were compared on a set of matched paired samples of the lichen Pseudevernia furfuracea, a widely used biomonitor for which BEC values based on partial digestion were previously provided. The standard reference material BCR 482 (P. furfuracea) was used to validate analytical procedures consisting of either a HF total mineralization or an aqua regia partial one, both associated to ICP-MS multi-element analysis. In particular, the performance of the procedures was evaluated by comparing analytical results of field samples with the accuracy obtained on BCR aliquots (measured-to-expected percentage ratio). The total digestion showed a better performance for Al, As, Ba, Ca, Cd, Cu, Fe, Mn, Ni, Se, Sn, and Zn, whereas the opposite was found for Cr, Co, P, and S. Moreover, new BEC values were provided for P. furfuracea using a consolidated statistical approach, after a total sample digestion with hydrofluoric acid. The multivariate investigation of the background variability of 43 elements in 57 remote Italian sites led to the identification of geographically homogeneous areas for which BEC values are provided for use as reference in biomonitoring applications.

Distribution of toxic elements and transfer from the environment to humans traced by using lead isotopes. A case of study in the Sarno River basin, south Italy.

The results of a large geochemical study on various environmental media (soil, stream sediment, groundwater, surface water, lettuce and human hair) of the Sarno River basin, which is one of the most polluted areas in Italy, are presented. Further, it aims to deepen our understanding of the distribution of Pb and its isotope composition for the differentiation between natural and anthropogenic metal sources. Our results show the environmental media to be significantly enriched in Cr, Cu, Pb, Hg, Zn, and to a lesser extent in Sb, Cd and Ni compared to the natural local background variation. The numerous industrial activities (mainly tanneries) have caused environmental pollution especially Cr and Hg in soils and sediment samples. Such contamination is also evident in lettuce and in the hair of the resident population, which shows particularly high values for both Cr and Hg. The unusually high As, Be and Sn concentrations arise mostly from natural sources due to the volcanic nature of the investigated area. Lead isotope measurements indicate a trend suggesting mixing between two end-members, one of clear natural origin (geogenic) and another related to human activities (anthropogenic). Lead isotope results demonstrate that Pb in hair of inhabitants is similar to those in the local topsoil and that gasoline is one of the main, but not the only source of metal pollution. The most important exposure risks within the study area are associated with toxic elements levels in topsoil and stream sediment, and the ingestion of locally grown lettuce. The high concentrations of these elements in hair are a further confirmation of this exposure pathway.

Strontium isotope signature of the PGI lemons Limone Costa d'Amalfi and Limone di Sorrento, and of the orchard soils from Sorrento peninsula.

The Limone Costa d'Amalfi and Limone di Sorrento lemons from the Sorrento Peninsula have Protected Geographical Indication (PGI) and are subject to origin fraud. The 87Sr/86Sr ratio (SrIR) signature of lemons and soils was investigated to verify its reliability to trace the PGI lemons and to highlight environmental factors responsible of the lemon SrIRs. The SrIR ranges of each PGI lemon were not discriminating as they overlapped with each other and some non-PGI lemon SrIRs fell within these ranges. The lemon SrIRs were generally not correlated with bulk and bioavailable soil SrIRs, rather, they were the result of plant Sr uptake with different SrIRs depending on interaction between water supplied to soil and soil with different chemical and physical characteristics. The study of lemon SrIRs and the causes of their origin and variability provides a reliable forecast reference for the other PGI agri-food products in the study area.

What the fish? Tracing the geographical origin of fish using NIR spectroscopy.

Food authentication is a growing concern with rising complexities of the food supply network, with fish being an easy target of food fraud. In this regard, NIR spectroscopy has been used as an efficient tool for food authentication. This article reviews the latest research advances on NIR based fish authentication. The process from sampling/sample preparation to data analysis has been covered. Special attention was given to NIR spectra pre-processing and its unsupervised and supervised analysis. Sampling is an important aspect of traceability study and samples chosen ought to be a true representative of the population. NIR spectra acquired is often laden with overlapping bands, scattering and highly multicollinear. It needs adequate pre-processing to remove all undesirable features. The pre-processing technique can make or break a model and thus need a trial-and-error approach to find the best fit. As for spectral analysis and modelling, multicollinear nature of NIR spectra demands unsupervised analysis (PCA) to compact the features before application of supervised multivariate techniques such as LDA, PLS-DA, QDA etc. Machine learning approach of modelling has shown promising result in food authentication modelling and negates the need for unsupervised analysis before modelling.

Impaired Perception of Body-Weight Distribution Marks Functional Mobility Problems in Patients Undergoing Total Hip Arthroplasty.

Hip osteoarthritis and total hip arthroplasty imply damaged articular and periarticular structures responsible for proprioception, and this damage may impair the accurate perception of body-weight distribution. In this study, we investigated proprioceptive abilities and accuracy perceiving body-weight distribution in patients undergoing total hip arthroplasty, and we assessed the associations between these abilities and body perception accuracy with functional mobility testing in 20 patients scheduled for total hip arthroplasty and 20 age-matched healthy participants. We assessed (a) absolute error in hip joint position sense (AE-JPS), (b) absolute error in body-weight distribution (AE-BWD) during standing and sit-to-stand tasks with open and closed eyes, and (c) functional mobility with the Timed Up and Go Test (TUG). We assessed patients undergoing hip arthroplasty before (T0) and five days after their surgery (T1), while control participants underwent a single evaluation. Relative to controls, participants undergoing surgery showed higher AE-JPS at 15° of hip flexion at T0 (p = .003) and at T1 (p = .007), greater AE-BWD during sit-to-stand with open eyes at T1 (p = .014) and with closed eyes at both T0 (p = .014) and at T1 (p < .001), and worse TUG at both T0 (p = .009) and T1 (p < .001). AE-BWD during sit-to-stand with closed eyes positively correlated with TUG at T0 (r = 0.55, p = .011) and at T1 (r = 0.51, p = .027). These findings suggested that impairments in body-weight distribution perception were evident both before and immediately after total hip arthroplasty, suggesting that these impairments may regularly mark these patients' functional mobility problems.

Long-term gait analysis in patients after total knee arthroplasty: A systematic review and meta-analysis.

BACKGROUND: Gait abnormalities have been described in patients after total knee arthroplasty (TKA), leading to the development of inter-joint coordination abnormalities and increased risk of falling. Such impairments have been reported to persist in the long-term, although the majority of studies assessed gait pattern especially in the first months after TKA. RESEARCH QUESTION: What are the long-term gait impairments in patients after TKA compared to healthy age-matched subjects? METHODS: A systematic search was conducted on MEDLINE/PubMed, EMBASE, CENTRAL and Scopus databases. Observational studies or randomized controlled trials investigating gait spatial-temporal, kinematic and kinetics parameters in a time-window longer than 6 months in patients with TKA compared to healthy age-matched subjects were included. Methodological quality was assessed using the modified Downs and Black (D&B) checklist and participants' characteristics, surgical procedures details and outcome measures were extracted. Pooled or un-pooled findings were categorized into "6 months - 1 year" and "more than 1 year" timepoint categories. RESULTS: Twenty-eight studies (976 patients) were included. Overall quality was fair with a mean modified D&B score of 63.5 %. Reduced speed, stride length, cadence and longer stance phase were found in patients when compared to healthy individuals at "6 months - 1 year" follow-up. Spatial-temporal parameters deficits were also found at more than 1 year after TKA, where lower single-limb support and longer double-limb support durations were detected. These impairments occurred in concomitance with decreased knee range of motion along the sagittal and frontal planes and altered kinetic parameters. Hip kinematic and kinetic long-term impairments were also detected after TKA. SIGNIFICANCE: These findings highlighted long-term gait pattern alterations in patients with TKA compared to age-matched healthy subjects. Future studies should identify interventions able to reduce long-term gait pattern alterations and improve function in patients after TKA.

Effects of Action Observation Plus Motor Imagery Administered by Immersive Virtual Reality on Hand Dexterity in Healthy Subjects.

Action observation and motor imagery (AOMI) are commonly delivered through a laptop screen. Immersive virtual reality (VR) may enhance the observer's embodiment, a factor that may boost AOMI effects. The study aimed to investigate the effects on manual dexterity of AOMI delivered through immersive VR compared to AOMI administered through a laptop. To evaluate whether VR can enhance the effects of AOMI, forty-five young volunteers were enrolled and randomly assigned to the VR-AOMI group, who underwent AOMI through immersive VR, the AOMI group, who underwent AOMI through a laptop screen, or the control group, who observed landscape video clips. All participants underwent a 5-day treatment, consisting of 12 min per day. We investigated between and within-group differences after treatments relative to functional manual dexterity tasks using the Purdue Pegboard Test (PPT). This test included right hand (R), left hand (L), both hands (B), R + L + B, and assembly tasks. Additionally, we analyzed kinematics parameters including total and sub-phase duration, peak and mean velocity, and normalized jerk, during the Nine-Hole Peg Test to examine whether changes in functional scores may also occur through specific kinematic patterns. Participants were assessed at baseline (T0), after the first training session (T1), and at the end of training (T2). A significant time by group interaction and time effects were found for PPT, where both VR-AOMI and AOMI groups improved at the end of training. Larger PPT-L task improvements were found in the VR-AOMI group (d: 0.84, CI95: 0.09-1.58) compared to the AOMI group from T0 to T1. Immersive VR used for the delivery of AOMI speeded up hand dexterity improvements.

The role of different acoustic environmental stimuli on manual dexterity.

Music has been reported to facilitate motor performance. However, there is no data on the effects of different acoustic environmental stimuli on manual dexterity. The present observational study aimed at investigating the effects of background music and noise on a manual dexterity task in young, middle-aged and elderly subjects. Sixty healthy, right-handed subjects aged between 18 and 80 years were enrolled. Twenty young (mean age: 22±2 years), 20 middle-aged (mean age: 55±8 years) and 20 elderly (mean age: 72±5 years) subjects performed the Nine Hole Peg Test (NHPT) in four different acoustic environments: silence (noise < 20dBA), classical music at 60dBA, rock music at 70 dBA, and a noise stimulus at 80dBA. Performance was recorded using an optical motion capture system and retro-reflective markers (SMART DX, 400, BTS). Outcome measures included the total test time and peg-grasp, peg-transfer, peg-in-hole, hand-return, and removing phases times. Normalized jerk, mean and peak of velocity during transfer and return phases were also computed. No differences were found for NHPT phases and total times, normalized jerk, peak of velocity and mean velocity between four acoustic conditions (p>0.05). Between-group differences were found for NHPT total time, where young subjects revealed better performance than elderly (p˂0.001) and middle-aged (p˂0.001) groups. Music and noise stimuli in the considered range of intensity had no influence on the execution of a manual dexterity task in young, middle-aged and elderly subjects. These findings may have implications for working, sportive and rehabilitative activities.

Assessment of Fertility Dynamics and Nutritional Quality of Potato Tubers in a Compost-Amended Mars Regolith Simulant.

Mars exploration will foresee the design of bioregenerative life support systems (BLSSs), in which the use/recycle of in situ resources might allow the production of food crops. However, cultivation on the poorly-fertile Mars regolith will be very challenging. To pursue this goal, we grew potato (Solanum tuberosum L.) plants on the MMS-1 Mojave Mars regolith simulant, pure (R100) and mixed with green compost at 30% (R70C30), in a pot in a cold glasshouse with fertigation. For comparison purposes, we also grew plants on a fluvial sand, pure (S100) and amended with 30% of compost (S70C30), a volcanic soil (VS) and a red soil (RS). We studied the fertility dynamics in the substrates over time and the tuber nutritional quality. We investigated nutrient bioavailability and fertility indicators in the substrates and the quality of potato tubers. Plants completed the life cycle on R100 and produced scarce but nutritious tubers, despite many critical simulant properties. The compost supply enhanced the MMS-1 chemical/physical fertility and determined a higher tuber yield of better nutritional quality. This study demonstrated that a compost-amended Mars simulant could be a proper substrate to produce food crops in BLSSs, enabling it to provide similar ecosystem services of the studied terrestrial soils.

Assessment of the environmental conditions of the Sarno river basin (south Italy): a stream sediment approach.

The Sarno river basin covers an area of 500 km(2) collecting the waters of Solofrana and Cavaiola tributaries. Originally it manly represents a source of livelihood for inhabitants by fishing and transporting goods; currently, the Sarno river, still partially used for irrigation, is affected by an extreme environmental degradation as a result of uncontrolled outflow of industrial waste. Within the framework of a wider geochemical prospecting project aiming at characterizing the whole territory of the Campania region, 89 stream sediment samples with a sampling density of 1 sample per 5 km(2) were collected in the river basin and analyzed by means of inductively coupled plasma-mass spectrometry in order to assess the environmental conditions at a regional scale. A GIS-aided technique, based on both the actual distribution of potentially harmful elements and their regional background values, was used to generate the maps of the contamination factors and of the contamination degrees for As, Cd, Cr, Cu, Hg, Pb and Zn. Furthermore, a factor analysis was performed to assess the nature and the extent of contamination sources for the river sediments. Results showed that the Sarno river basin could be divided in two "environmental status" units: one, low contaminated, corresponding to the hilly and mountain areas, and the second, from moderately to very highly contaminated, corresponding to the economically developed areas of the valley floor characterized by a high population density. This work was developed within a project that aims to investigate the relationships between environmental pollution and human health by analyzing environmental media (stream sediments, water, soil and vegetation) together with human hair of resident population. In this context, the spatial correlation between the extremely compromised environmental conditions of developed areas and the incidence rate of liver cancer in the same area was also explored posing the need of a careful costs/benefits analysis to assess whether the deterioration of the environment, that could adversely affect the conditions of public health, is worth the economic development.

Joint pressure stimuli increase quadriceps strength and neuromuscular activity in patients with knee osteoarthritis.

The study investigated the effects of periarticular knee pressure stimuli on quadriceps strength and neuromuscular activity in subjects with knee osteoarthritis. Twenty-five subjects with knee osteoarthritis and 25 age-matched healthy controls performed maximal voluntary knee extension tasks on an isometric dynamometer. Three different pressure stimuli (no-pressure, 60-mmHg, 120-mmHg) were applied using a sphygmomanometer via the cuff covering the knee joint. Peak torque and root-mean-square peak of rectus femoris (RF), vastus medialis (VM), and vastus lateralis (VL) were collected and normalized for the no-pressure condition (nTorque-peak and nRMS-peak). Normalized Torque-peak increased from no-pressure to 60-mmHg and 120-mmHg in patients, which revealed higher nTorque-peak during 60-mmHg (MD: 10.9%, IC95: 1.8%, 20.1%, p = 0.020) and 120-mmHg (MD: 16.0%, IC95: 4.4%, 27.6%, p = 0.008) conditions than healthy subjects. Moreover, nRMS-peak increased from no-pressure to 60-mmHg for RF, from no-pressure to 120-mmHg for RF, VM and VL, and from 60-mmHg to 120 mm-Hg for VL in patients. Patients revealed higher nRMS-peak of RF and VM during 60-mmHg and 120-mmHg conditions than healthy subjects. Periarticular knee pressure stimuli enhanced quadriceps strength and neuromuscular activity in subjects with knee osteoarthritis. This approach may represent a new strength training modality in patients with neuromuscular activation deficits for knee osteoarthritis.

Childhood overweight and obesity in Bariloche, Argentina: Beyond eating habits and physical activity. / Sobrepeso y obesidad en infancias de Bariloche, Argentina: más allá de la alimentación y la actividad física.

The incidence of overweight and obesity is alarming worldwide. In Argentina, multifactorial studies are scarce despite the high rates of childhood overweight and obesity (OW/OB). In this study we assessed the prevalence of childhood OW/OB and the associated risk factors from a multicausal perspective: we analyzed sleep habits, screen use, chronic stress in children and their caregivers, social relationship problems, eating habits, and physical activity. Of the 397 first and seventh graders who completed the questionnaires, 41% had childhood OW/OB. In children with obesity, a lower level of physical activity, a lower consumption of protective foods, a higher risk for sleep disorders, higher levels of chronic stress (in both children and their caregivers), and higher rates of peer rejection were observed. Widespread use of screens before bedtime was noted in most children. This study evidences the diversity of factors associated with childhood OW/OB and highlights the convenience of a multidimensional approach to address it. In addition, the results of this study suggest the need to consider lifestyle habits as a preventive and therapeutic strategy for the management of OW/OB from early stages.

La incidencia de sobrepeso y obesidad es alarmante en el mundo. En Argentina son escasos los estudios multifactoriales a pesar de los altos índices de sobrepeso y obesidad en la población infantil (SOI). En este estudio evaluamos la prevalencia de SOI y sus factores de riesgo asociados desde una perspectiva multicausal: analizamos hábitos de sueño, uso de pantallas, estrés crónico en niños y sus cuidadores, problemas de relación social, alimentación y actividad física. De los 397 niños de primer y séptimo grado que completaron los cuestionarios, el 41 % presentó SOI. Los niños con obesidad mostraron realizar menos actividad física, tener un menor consumo de alimentos protectores, más riesgos de trastornos del sueño, mayores niveles de estrés crónico (tanto en los niños como en sus cuidadores) y mayor índice de rechazo entre pares. En la mayoría de los niños se encontró un uso generalizado de pantallas antes de acostarse. El presente estudio evidencia la diversidad de factores asociados a esta problemática y destaca la conveniencia de utilizar un enfoque multidimensional para abordar la obesidad en la infancia. Asimismo, los resultados de este trabajo sugieren la necesidad de considerar los hábitos de vida como estrategia preventiva y terapéutica para el tratamiento de esta enfermedad desde etapas tempranas.