Reinforcing effect of ε-polylysine-carboxymethyl chitosan nanoparticles on gelatin-based film: Enhancement of physicochemical, antioxidant, and antibacterial properties.

The development and application of antibacterial film were highly anticipated to prevent food spoilage caused by bacteria. In this investigation, antibacterial and antioxidant functionalized gelatin-based film was formed with the incorporation of oregano essential emulsion Pickering emulsion (OPE). ε-Polylysine-Carboxymethyl Chitosan nanoparticles (CMCS-ε-PL) composed of different mass ratios of CMCS and ε-PL were orchestrated by electrostatic forces and hydrogen bonding, which effectively acted as a stabilizer for OPE. The design of different mass ratios of CMCS and ε-PL in CMCS-ε-PL has a deep effect on the structure and functional properties of OPE and film. It successfully improved the encapsulation efficiency of OPE from 49.52 % to 79.83 %. With the observation of AFM images, the augmentation of surface roughness consequent to OPE incorporation can be relieved by the increased contention of ε-PL in CMCS-ε-PL. Meanwhile, the mechanical properties, barrier properties, anti-oxidation, and antibacterial properties of the films were improved with the incorporation of the above OPE. In particular, a synergistic antibacterial activity between ε-PL and OEO in the film was demonstrated in this study and the mechanism of enhanced antibacterial activity was elucidated by examining the integrity of bacteria cell membrane. The film unequivocally demonstrated its ability to appreciably prolong the shelf life of both beef and strawberries with excellent antioxidant and antibacterial properties.

Salt reduction in myofibrillar protein gel via inhomogeneous distribution of sodium-containing encapsulated fish oil coacervate: Mucopenetration ability of sodium carboxymethyl cellulose.

The potential application of fish oil microcapsules as salt reduction strategies in low-salt myofibrillar protein (MP) gel was investigated by employing soy protein isolates/carboxymethyl cellulose sodium (SPI-CMC) coacervates enriched with 25 mM sodium chloride and exploring their rheological characteristics, taste perception, and microstructure. The results revealed that the SPI-CMC coacervate phase exhibited the highest sodium content under 25 mM sodium level, albeit with uneven distribution. Notably, the hydrophilic and adhesive properties of CMC to sodium facilitated the in vitro release of sodium during oral digestion, as evidenced by the excellent wettability and mucopenetration ability of CMC. Remarkably, the fish oil microcapsules incorporating SPI-CMC as the wall material, prepared at pH 3.5 with a core-to-wall ratio of 1:1, demonstrated the highest encapsulation efficiency, which was supported by the strong hydrogen bonding. Interestingly, the presence of SPI-CMC coacervates and fish oil microcapsules enhanced the interaction between MPs and strengthened the low-salt MP gel network. Coupled with electronic tongue analysis, the incorporation of fish oil microcapsules slightly exacerbated the non-uniformity of sodium distribution. This ultimately contributed to an enhanced perception of saltiness, richness, and aftertaste in low-salt protein gels. Overall, the incorporation of fish oil microcapsules emerged as an effective salt reduction strategy in low-salt MP gel.

Photodynamic-responsive gelatin-based coating with high utilization curcumin loaded bilayer nanoencapsulation: A promising environmental food packaging.

The development and application of curcumin-mediated antimicrobial photodynamic techniques (PDT) in food preservation are highly anticipated to resist microbial contamination and prevent food spoilage. In this study, high-utilization curcumin-loaded bilayer nanoencapsulation was prepared to incorporated into a gelatin-based edible coating for beef preservation. Bilayer nanoencapsulation composed of shellac and poly-γ-glutamic acid (CS-NPs) improved the encapsulation efficiency of shellac to curcumin by >1.5 times. The incorporation of CS-NPs improved the compact of coating structure with hydrogen bonds. In food simulants, coatings possessed control release properties and the release mechanism was Fick diffusion (without the addition of γ-PGA) and non-Fick diffusion (with the addition of γ-PGA). These prepared coatings exhibited excellent barrier, antibacterial (antibacterial ratio > 95 %), and antioxidant properties (scavenging ratio > 90 %). Curcumin mediated antimicrobial photodynamic techniques (PDT) of the coatings were verified with the activity of blue light-induced reactive oxygen species (ROS). The shelf-life of beef was extended by the coating with blue light. In summary, the design of bilayer CS-NPs significantly improved the utilization of curcumin which provided a high-efficiency strategy for PDT-responsive food packaging with environmental practical.

Enhanced environmental stress resistance and functional properties of the curcumin-shellac nano-delivery system: Anti-flocculation of poly-γ-glutamic acid.

Curcumin was widely designed as nanoparticles to remove application restrictions. The occurrence of flocculation is a primary factor limiting the application of the curcumin nano-delivery system. To enhance the environmental stress resistance and functional properties of shellac-curcumin nanoparticles (S-Cur-NPs), γ-polyglutamic acid (γ-PGA) was utilized as an anti-flocculant. The encapsulation efficiency and loading capacity of S-Cur-NPs were also improved with γ-PGA incorporation. FTIR and XRD analysis confirmed the presence of amorphous characteristics in S-Cur-NPs and the combination of γ-PGA and shellac was driven by hydrogen bonding. The hydrophilic, thermodynamic, and surface potential of S-Cur-NPs was improved by the incorporation of γ-PGA. This contribution of γ-PGA on S-Cur-NPs effectively mitigated the flocculation occurrence during heating, storage, and in-vitro digestive treatment. Furthermore, it was revealed that γ-PGA enhanced the antibacterial and antioxidant properties of S-Cur-NPs and effectively protected the functional activity against heating, storage, and in-vitro digestion. Release studies conducted in simulated gastrointestinal fluids revealed that S-Cur-NPs have targeted intestinal release properties. Overall, the design of shellac with γ-PGA was a promising strategy to relieve the application stress of shellac and curcumin in the food industry.

The synergistic effect of polyphenols and polypeptides for plant-based bioplastic film - Enhanced UV resistance, antioxidant and antibacterial performance.

The exceptional biodegradability and active biological functions of bio-based packaging materials have attracted increasing interest. In this study, a bioplastic film was developed by introducing simultaneously polyphenols (tea polyphenols, TPs) and peptides (nisin) into a soy protein isolate/sodium alginate (SPI/SA) based film-forming matrix. The research results revealed that the dynamic coordinated interaction between TPs and nisin enhanced mechanical properties, UV-resistance, and thermal stability of bioplastic films. Furthermore, the bioplastic film exhibited antibacterial activity and antioxidant properties. Significantly, biofilm growth of Staphylococcus aureus treated with TPs-5/Nisin-5 bioplastic film was inhibited by 91.12% compared to the blank group. The shelf life of beef with TPs-5/Nisin-5 bioplastic film was prolonged by 2 days because of the synergistic effect of TPs and nisin. Additionally, the bioplastic film biodegraded in the natural environment about 21 days. This environmentally friendly regeneration strategy and the integration of advantageous functions provided ideas for the development of active food packaging.

Modification of low-salt myofibrillar protein using combined ultrasound pre-treatment and konjac glucomannan for improving gelling properties: Intermolecular interaction and filling effect.

The quality deterioration of low-salt meat products has been gained ongoing focus of researchers. In this study, konjac glucomannan (KGM) was used to alleviate the finiteness of ultrasound treatment on the quality improvement of low-salt myofibrillar protein (MP), and the modification sequence was also investigated. The results revealed that the single and double sequential modification by utilizing KGM and ultrasound significantly influenced the gelation behavior of low-salt MPs. The uniform MP-KGM mixture formed by a single ultrasound treatment had limited protein unfolding, resulting in relatively weak intermolecular forces in the composite gel. Importantly, ultrasound pre-treatment combined with KGM modification promoted the unfolding and moderate thermal aggregation of proteins and remarkably improved the rheological behaviors and gel strength of the composite gel. This result could also be corroborated by the highest percentage of trans-gauche-trans conformation of SS bridges and maximum ß-sheet proportion. Furthermore, molecular dynamic simulation and molecular docking elucidated that the hydrogen bond length between protein and KGM was shortened after ultrasound pre-treatment, which was the molecular basis for the enhanced intermolecular interactions. Therefore, ultrasound pre-treatment combined with KGM can effectively improve the gelling properties of low-salt MPs, providing a practical method for the processing of low-salt meat products.

A curcumin-crosslinked bilayer film of soy protein isolate and chitosan with enhanced antibacterial property for beef preservation and freshness monitoring.

In this study, antibacterial and antioxidant bilayer films were prepared by using curcumin (Cur) crosslinked soy rotein isolate (SPI) and chitosan (CS). Molecular docking simulations and multispectral analysis revealed that hydrogen bonding and hydrophobic interactions were the primary driving forces that promoted the self-assembly of the bilayer films. The tensile strength, the UV-blocking properties and the hydrophobicity was greatly improved of the bilayer antimicrobial films. Moreover, water vapor permeability, thermal shrinkage and opacity were all reduced significantly. In addition, the composite films with curcumin demonstrated effective antioxidant activity and a slow release characteristic. Morphology observation of the bacteria by AFM revealed that the antibacterial bilayer film had a significant damaging effect on the cell structures of S. aureus and E. coli due to the dual antibacterial effect of curcumin and chitosan. SPI + Cur-CS antimicrobial bilayer film effectively inhibited the growth of bacteria and extended the shelf life of beef. According to the findings, SPI + Cur-CS antimicrobial bilayer film can be used as an active package material for beef preservation and freshness monitoring.