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Cadmium (Cd), which accumulates in tobacco leaves, enters the human body through inhalation of smoke, causing harmful effects on health. Therefore, identifying the pivotal factors that govern the absorption and resistance of Cd in tobacco is crucial for mitigating the harmful impact of Cd. In the present study, four different Cd-sensitive varieties, namely, ZhongChuan208 (ZC) with resistance, ZhongYan100 (ZY), K326 with moderate resistance, and YunYan87 (YY) with sensitivity, were cultivated in hydroponic with different Cd concentrations (20⯵M, 40⯵M, 60⯵M and 80⯵M). The results indicated that plant growth was significantly decreased by Cd. Irrespective of the Cd concentration, ZC exhibited the highest biomass, while YY had the lowest biomass; ZY and K326 showed intermediate levels. Enzymatic (APX, CAT, POD) and nonenzymatic antioxidant (Pro, GSH) systems showed notable variations among varieties. The multifactor analysis suggested that the ZC and ZY varieties, with higher levels of Pro and GSH content, contribute to a decrease in the levels of MDA and ROS. Among all the Cd concentrations, ZC exhibited the lowest Cd accumulation, while YY showed the highest. Additionally, there were significant differences observed in Cd distribution and translocation factors among the four different varieties. In terms of Cd distribution, cell wall Cd accounted for the highest proportion of total Cd, and organelles had the lowest proportion. Among the varieties, ZC showed lower Cd levels in the cell wall, soluble fraction, and organelles. Conversely, YY exhibited the highest Cd accumulation in all tissues; K326 and ZY had intermediate levels. Translocation factors (TF) varied among the varieties under Cd stress, with ZC and ZY showing lower TF compared to YY and K326. This phenomenon mainly attributed to regulation of the NtNramp3 and NtNramp5 genes, which are responsible for the absorption and transport of Cd. This study provides a theoretical foundation for the selection and breeding of tobacco varieties that are resistant to or accumulate less Cd.
Assuntos
Nicotiana , Nicotiana/química , Nicotiana/genética , Nicotiana/crescimento & desenvolvimento , Nicotiana/metabolismo , Cádmio/toxicidade , Resistência a Medicamentos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacos , Antioxidantes/metabolismo , Proteínas de Transporte de Cátions/metabolismoRESUMO
While it is widely recognized that hydrogen sulfide (H2S) promotes plant stress tolerance, the precise processes through which H2S modulates this process remains unclear. The processes by which H2S promotes phosphorus deficiency (PD) and salinity stress (SS) tolerance, simulated individually or together, were examined in this study. The adverse impacts on plant biomass, total chlorophyll and chlorophyll fluorescence were more pronounced with joint occurrence of PD and SS than with individual application. Malondialdehyde (MDA), hydrogen peroxide (H2O2), and electrolyte leakage (EL) levels in plant leaves were higher in plants exposed to joint stresses than in plants grown under an individual stress. When plants were exposed to a single stress as opposed to both stressors, sodium hydrosulfide (NaHS) treatment more efficiently decreased EL, MDA, and H2O2 concentrations. Superoxide dismutase, peroxidase, glutathione reductase and ascorbate peroxidase activities were increased by SS alone or in conjunction with PD, whereas catalase activity decreased significantly. The favorable impact of NaHS on all the evaluated attributes was reversed by supplementation with 0.2 mM hypotaurine (HT), a H2S scavenger. Overall, the unfavorable effects caused to NaHS-supplied plants by a single stress were less severe compared with those caused by the combined administration of both stressors.
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Capsicum , Sulfeto de Hidrogênio , Sulfetos , Sulfeto de Hidrogênio/farmacologia , Peróxido de Hidrogênio , Antioxidantes , Clorofila , Suplementos Nutricionais , Fosfatos , PlântulaRESUMO
Microplastic accumulation in the soil-plant system can stress plants and affect products quality. Currently, studies on the effect of microplastics on plants are not consistent and underlying molecular mechanisms are yet unknown. Here for the first time, we performed a study to explore the molecular mechanism underlying the growth of soybean plants in soil contaminated with various types of microplastics (PS and HDPE) and arbuscular mycorrhizal fungi (AMF) (presence/absence). Our results revealed that a dose-dependent decline was observed in plant growth, chlorophyll content, and yield of soybean under MPs stress. The addition of MPs resulted in oxidative stress closely related to hydrogen peroxide generation (H2O2), methylglyoxal (MG) levels, lipid peroxidation (MDA), and lipoxygenase (LOX). In contrast, MPs addition enhanced mycorrhizal colonization and dependency relative to control while the rubisco and root activity declined. All the genes (GmHMA13 and GmHMA19) were downregulated in the presence of MPs except GmHMA18 in roots. AMF inoculation alleviated MPs-induced phytotoxic effects on colonization, rubisco activity, root activity and restored the growth of soybean. Under MPs exposure, AMF inoculation induced plant defense system via improved regulation of antioxidant enzymes, ascorbate, glutathione pool, and glyoxalase system. AMF upregulated the genes responsible for metals uptake in soybean under MPs stress. The antioxidant and glyoxalase systems coordinated regulation expressively inhibited the oxidative and carbonyl stress at both MPs types. Hence, AMF inoculation may be considered an effective approach for minimizing MPs toxicity and its adverse effects on growth of soybean grown on MPs-contaminated soils.
Assuntos
Micorrizas , Micorrizas/fisiologia , Microplásticos , Plásticos , Antioxidantes , Glycine max/genética , Raízes de Plantas , Peróxido de Hidrogênio , Ribulose-Bifosfato Carboxilase , Plantas , SoloRESUMO
Microplastic (MP) contamination poses a substantial threat to agroecosystems, disrupting soil properties, nutrient cycles, and microbial communities and ultimately affecting plant growth and ecosystem resilience. The effects of straw addition on the storage of soil organic carbon (SOC) and greenhouse gas emissions have been extensively explored, but these effects have not been examined in the context of MP contamination. To assess the impacts of legume straw and polyethylene microplastics on SOC fractions and carbon dioxide (CO2) and nitrous oxide (N2O) emissions, 7-month soil incubation experiments were performed. The results revealed that the inclusion of legume straw in soil considerably increased microbial SOC compared to the control. However, straw addition to MP-contaminated soil reduced microbial SOC compared to that in soil containing only straw. In contrast, the addition of straw to MP-contaminated soil elevated (+44%) the SOC mineral relative to the sole application of straw. Intriguingly, straw incorporation into MP-contaminated soil reduced microbial biomass carbon and nitrogen relative to soil containing only straw. Straw addition to MP-contaminated soil enhanced the nitrification activity and reduced the relative expression of AOBamoABC gene compared to sole straw-incorporated soil and the control. Greenhouse gas emissions were also modulated; for instance, straw incorporation into MP-contaminated soil reduced CO2 and N2O emissions by -11% and -46%, compared to straw incorporation alone. The urease and phosphatase activities were decreased (-58% and -12%) in the MP-polluted soil with straw incorporation compared with those in the soil in which only straw was applied. However, invertase and catalase activities were upregulated in the straw-incorporated soil contaminated with MPs. Straw addition in the MP-polluted soil considerably enhanced (+2%) the microbial community structure (indicated by PLFA) compared to the sole straw application. These results provide a comprehensive perspective on the role of legume straw incorporation in addressing MP pollution, showcasing its potential for sustainable agricultural practices in the face of evolving environmental challenges.
Assuntos
Gases de Efeito Estufa , Microbiota , Solo/química , Microplásticos , Plásticos , Dióxido de Carbono/análise , Carbono , Agricultura/métodos , Óxido Nitroso/análise , VerdurasRESUMO
Microplastics (MPs) accumulation in terrestrial ecosystems can affect greenhouse gases (GHGs) production by altering microbial and soil structure. Presently, research on the MPs effect on plants is not consistent, and underlying molecular mechanisms associated with GHGs are yet unknown. For the first time, we conducted a microcosm study to explore the impact of MPs addition (Raw vs. aged) and Trichoderma longibrachiatum and Bacillus subtilis inoculation (Sole vs. combination) on GHGs emission, soil community structure, physiochemical properties, and enzyme activities. Our results indicated that the addition of aged MPs considerably enhanced the GHGs emissions (N2O (+16%) and CO2 (+21%), respectively), C and N cycling gene expression, microbial biomass carbon, and soil physiochemical properties than raw MPs. However, the soil microbial community structure and enzyme activities were enhanced in raw MPs added treatments, irrespective of the MPs type added to soil. However, microbial inoculation significantly reduced GHGs emission by altering the expression of C and N cycling genes in both types of MPs added treatments. The soil microbial community structure, enzymes activities, physiochemical properties and microbial biomass carbon were enhanced in the presence of microbial inoculation in both type of MPs. Among sole and combined inoculation of Trichoderma and Bacillus subtilis, the co-applied Trichoderma and Bacillus subtilis considerably reduced the GHGs emission (N2O (-64%) and CO2 (-61%), respectively) by altering the expression of C and N cycling genes regardless of MPs type used. The combined inoculation also enhanced soil enzyme activities, microbial community structure, physiochemical properties and microbial biomass carbon in both types of MPs treatment. Our findings provide evidence that polyethylene MPs likely pose a high risk of GHGs emission while combined application of Trichoderma and Bacillus subtilis significantly reduced GHGs emission by altering C and N cycling gene expression, soil microbial community structure, and enzyme activities under MPs pollution in a terrestrial ecosystem.
Assuntos
Gases de Efeito Estufa , Microbiota , Gases de Efeito Estufa/análise , Solo/química , Microplásticos , Plásticos , Dióxido de Carbono/análise , Carbono , Bactérias , Óxido Nitroso/análiseRESUMO
Cadmium (Cd) is readily absorbed by tobacco and accumulates in the human body through smoke inhalation, posing threat to human health. While there have been many studies on the negative impact of cadmium in tobacco on human health, the specific adaptive mechanism of tobacco roots to cadmium stress is not well understood. In order to comprehensively investigate the effects of Cd stress on the root system of tobacco, the combination of transcriptomic, biochemical, and physiological methods was utilized. In this study, tobacco growth was significantly inhibited by 50 µM of Cd, which was mainly attributed to the destruction of root cellular structure. By comparing the transcriptome between CK and Cd treatment, there were 3232 up-regulated deferentially expressed genes (DEGs) and 3278 down-regulated DEGs. The obvious differential expression of genes related to the nitrogen metabolism, metal transporters and the transcription factors families. In order to mitigate the harmful effects of Cd, the root system enhances Cd accumulation in the cell wall, thereby reducing the Cd content in the cytoplasm. This result may be mediated by plant hormones and transcription factor (TF). Correlational statistical analysis revealed significant negative correlations between IAA and GA with cadmium accumulation, indicated by correlation coefficients of -0.91 and -0.93, respectively. Conversely, ABA exhibited a positive correlation with a coefficient of 0.96. In addition, it was anticipated that 3 WRKY TFs would lead to a reduction in Cd accumulation. Our research provides a theoretical basis for the systematic study of the specific physiological processes of plant roots under Cd stress.
Assuntos
Cádmio , Reguladores de Crescimento de Plantas , Fatores de Transcrição , Transcriptoma , Cádmio/toxicidade , Cádmio/metabolismo , Fatores de Transcrição/metabolismo , Fatores de Transcrição/genética , Transcriptoma/efeitos dos fármacos , Reguladores de Crescimento de Plantas/metabolismo , Nicotiana/genética , Nicotiana/efeitos dos fármacos , Estresse Fisiológico , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacosRESUMO
In the original publication [...].
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MAIN CONCLUSION: Drought may be efficiently managed using the following strategies: prevention, mitigation, readiness, recovery, and transformation. Biotechnological interventions may become highly important in reducing plants' drought stress in order to address key plant challenges such as population growth and climate change. Drought is a multidimensional construct with several triggering mechanisms or contributing factors working at various spatiotemporal scales, making it one of the known natural catastrophes. Drought is among the causes of hunger and malnutrition, decreasing agricultural output, and poor nutrition. Many deaths caused in children are due to hunger situations, and one in four children face stunted growth. All this hunger and malnutrition may be responsible for the reduction in agricultural productivity caused due to the drought situations affecting food security. Global Hunger Index has been accelerating due to under-nutrition and under-5 deaths. Drought has been covering more than 20% of the world's agricultural areas, leading to significantly less food production than what is required for consumption. Drought reduces soil fertility and adversely affects soil biological activity reducing the inherent capacity of the soil to support vegetation. Recent droughts have had a much greater effect on people's lives, even beyond causing poverty and hunger. Drought may have substantial financial consequences across the globe it may cause a severe impact on the world economy. It is a natural feature of the environment that will appear and disappear as it has in history. Due to increasing temperatures and growing vulnerabilities, it will undoubtedly occur more often and seriously in the coming years. To ensure sustainable socio-economic and social development, it is critical to reducing the effects of potential droughts worldwide using different biotechnological interventions. It's part of a long-term growth plan, and forecasting is essential for early warnings and global hunger management.
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Fome , Desnutrição , Secas , Abastecimento de Alimentos , Desnutrição/epidemiologia , Desnutrição/prevenção & controle , SoloRESUMO
Metalloids in plants have diverse physiological effects. From being essential to beneficial to toxic, they have significant effects on many physiological processes, influencing crop yield and quality. Aquaporins are a group of membrane channels that have several physiological substrates along with water. Metalloids have emerged as one of their important substrates and they are found to have a substantial role in regulating plant metalloid homeostasis. The present review comprehensively details the multiple isoforms of aquaporins having specificity for metalloids and being responsible for their influx, distribution or efflux. In addition, it also highlights the usage of aquaporin-mediated transport as a selection marker in toxic screens and as tracer elements for closely related metalloids. Therefore, aquaporins, with their imperative contribution to the regulation of plant growth, development and physiological processes, need more research to unravel the metalloid trafficking mechanisms and their future applications.
Assuntos
Aquaporinas , Metaloides , Aquaporinas/metabolismo , Transporte Biológico , Metaloides/metabolismo , Proteínas de Plantas/metabolismo , Plantas/metabolismoRESUMO
The growth and persistence of rhizobacteria in soils are highly impacted by moisture stress. In this study, we report the first transcript analysis of four Pseudomonas strains (PS1, PS2, PS3, and PS4) isolated from the root-soil interface of rice and maize associated with different moisture levels during water deprivation. Filtered Pseudomonas sp. cells incubated at low (RH10%) and high (RH85%) relative humidity showed decreased survival of all Pseudomonas sp. at RH10% when compared with RH85%. RT-PCR showed differential expression of treS (trehalose synthase), rpoS (sigma factor), mucA (alginate regulatory gene), and fliM (flagellar motor switch protein gene) in response to exposure to RH10%. However, molecular fingerprinting and nutrient assimilation profile of Pseudomonas strains demonstrated genetic and physiological variation between the four strains irrespective of water regime and host. In vitro testing of these strains showed ACC deaminase activity and gibberellic acid, abscisic acid, indole acetic acid, and exopolysaccharide production. We determined that 50 µl of 1.2 × 103 CFU ml-1 of these Pseudomonas strains was enough to protect Arabidopsis plants against drought stress in a pot experiment. Inoculated plants increased their root colonization ability and biomass; however, PS2 showed higher survival (95%), relative water content (59%), chlorophyll (30%), glycine betaine (38%), proline (23%), and reduced MDA (43%) in shoots than irrigated control under induced water deprivation. It can be concluded that all Pseudomonas strains were effective in mitigating drought stress, however, PS2 appears to impart more resistance to drought than the other strains by upregulating key defense mechanisms.
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Arabidopsis , Secas , Ácido Abscísico/metabolismo , Arabidopsis/metabolismo , Pseudomonas/genética , Solo , Estresse FisiológicoRESUMO
KEY MESSAGE: A detailed study of the response of wheat plants, inoculated with drought-tolerant PGPR is studied which would be beneficial to achieve genetic improvement of wheat for drought tolerance. Drought stress, a major challenge under current climatic conditions, adversely affects wheat productivity. In the current study, we observed the response of wheat plants, inoculated with drought-tolerant plant growth-promoting rhizobacteria (PGPR) Bacillus megaterium (MU2) and Bacillus licheniformis (MU8) under induced drought stress. In vitro study of 90 rhizobacteria exhibited 38 isolates showed one or more plant growth-promoting properties, such as solubilization of phosphorus, potassium, and exopolysaccharide production. Four strains revealing the best activities were tested for their drought-tolerance ability by growing them on varying water potentials (- 0.05 to - 0.73 MPa). Among them, two bacterial strains Bacillus megaterium and Bacillus licheniformis showed the best drought-tolerance potential, ACC deaminase activities, IAA production, and antagonistic activities against plant pathogens. Additionally, these strains when exposed to drought stress (- 0.73 MPa) revealed the induction of three new polypeptides (18 kDa, 35 kDa, 30 kDa) in Bacillus megaterium. We determined that 106 cells/mL of Bacillus megaterium and Bacillus licheniformis were enough to induce drought tolerance in wheat under drought stress. These drought-tolerant strains increased the germination index (11-46%), promptness index (16-50%), seedling vigor index (11-151%), fresh weight (35-192%), and dry weight (58-226%) of wheat under irrigated and drought stress. Moreover, these strains efficiently colonized the wheat roots and increased plant biomass, relative water content, photosynthetic pigments, and osmolytes. Upon exposure to drought stress, Bacillus megaterium inoculated wheat plants exhibited improved tolerance by enhancing 59% relative water content, 260, 174 and 70% chlorophyll a, b and carotenoid, 136% protein content, 117% proline content and 57% decline in MDA content. Further, activities of defense-related antioxidant enzymes were also upregulated. Our results revealed that drought tolerance was more evident in Bacillus megaterium as compared to Bacillus licheniformis. These strains could be effective bioenhancer and biofertilizer for wheat cultivation in arid and semi-arid regions. However, a detailed study at the molecular level to deduce the mechanism by which these strains alleviate drought stress in wheat plants needs to be explored.
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Bacillus megaterium , Secas , Clorofila A/metabolismo , Raízes de Plantas/metabolismo , Triticum/metabolismo , Água/metabolismoRESUMO
Forests have been undergoing through immense pressure due to the factors like human activities; procurement of forest products and climate change which is a major factor influencing this pressure buildup on forests. Climate change and temperature increase caused by anthropogenic activities have notably affected forests and wildlife on a global scale. High temperature increases the soil-water evaporation, resulting in drier soils, and water loss in forest flora. The incidence of forest fires has doubled since 1984 and these are linked to global warming. Drought influences fuel moisture by bringing about physiological changes in forest vegetation leading to forest fires. Forest resilience is hampered because of temperature and drought stress at the developing stage of plant's life cycle leading to the shift in plant species in those areas. Forest fire incidences can be managed with proper management strategies such as sustainable, community and urban forest management. A careful monitoring of stress precursors, subsistence uses of forests, ecological education and planting of near native and new indigenous plant species are the tools that can aid in efficient forest management.
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Incêndios , Incêndios Florestais , Mudança Climática , Secas , Florestas , Humanos , ÁrvoresRESUMO
Maize is a low-temperature (LT)-sensitive plant and its physiological responses towards LT of temperate regions developed is an adaptive trait. To further our understanding about the response of maize to LT at the physiological and photosynthesis level, we conducted Infrared Gas Analysis (IRGA using LICOR6400-XT in 45-day-old grown two maize genotypes, one from temperate region (Gurez-Kashmir Himalayas), viz., Gurez local (Gz local), and another from tropics (Gujarat), viz., GM6. This study was carried out to evaluate the underlying physiological mechanisms in the two differentially temperature-tolerant maize genotypes. Net photosynthetic rate (A/PN), 18.253 in Gz local and 25.587 (µmol CO2 m-2 s-1) in GM6; leaf conductance (gs), 0.0102 in Gz local and 0.0566 (mmol H2O m-2 s-1) in GM6; transpiration rate (E), 0.5371 in Gz local and 2.9409 (mmol H2O m-2 s-1) in GM6; and water use efficiency (WUE), 33.9852 in Gz local and 8.7224 (µmol CO2 mmol H2O-1) in GM6, were recorded under ambient conditions. Also, photochemical efficiency of photosystem II (PSII) (Fv/Fm), 0.675 in Gz local and 0.705 in GM6; maximum photochemical efficiency (Fv'/Fm'), 0.310234 in Gz local and 0.401391 in GM6; photochemical quenching (qP), 0.2375 in Gz local and 0.2609 in GM6; non-photochemical quenching (NPQ), 2.0036 in Gz local and 1.1686 in GM6; effective yield of PSII (ФPSII), 0.0789 in Gz local and 0.099 in GM6; and electron transport rate (ETR), 55.3152 in Gz local and 68.112 in GM6, were also evaluated in addition to various response curves, like light intensities and temperature. We observed that light response curves show the saturation light intensity requirement of 1600 µmol for both the genotypes, whereas temperature response curves showed the optimum temperature requirement for Gz local as 20 °C and for GM6 it was found to be 35 °C. The results obtained for each individual parameter and other correlational studies indicate that IRGA forms a promising route for quick and reliable screening of various stress-tolerant valuable genotypes, forming the first study of its kind.
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Clorofila , Zea mays , Fluorescência , Genótipo , Fotossíntese/genética , Folhas de Planta/genética , Temperatura , Zea mays/genéticaRESUMO
Environmental contamination by hydrocarbons is a major problem, and hydrocarbon accumulation in soil poses hazardous threat to ecosystems. Phytoremediation, which involves plants, is an encouraging technique for the removal of hydrocarbons from polluted soil and water. The purpose of this investigation was to examine whether bacterial inoculation enhanced the phytoremediation of hydrocarbons in diesel-contaminated soil vegetated with maize (Zea mays L.). The two cultivars of maize, MMRI Yellow and Pearl White, were planted in diesel-polluted soil (0, 1.5, 2.5, and 3.5 g diesel kg-1 soil), and inoculated with the consortium of three alkane-degrading bacterial strains, Arthrobacter oxydans ITRH49, Pseudomonas sp. ITRI73 and Pseudomonas sp. MixRI75. Bacterial inoculation enhanced plant growth and hydrocarbon degradation. Between two cultivars, MMRI Yellow showed better growth and hydrocarbon degradation in the presence and absence of bacterial inoculation. Maximum hydrocarbon degradation (80%) was observed in the soil having minimum concentration of diesel (1.5 g kg-1 soil), and vegetated with bacterial inoculated MMRI Yellow maize cultivar. Furthermore, more bacterial colonization, and abundance and expression of the alkane hydroxylase gene (alkB) were observed in the root interior than in the rhizosphere and shoot interior of the plants. The bacteria-mediated phytoremediation of soil contaminated with hydrocarbons suggested that the collective use of plants and bacteria was the most beneficial approach for the reclamation of diesel-contaminated soil in comparison with vegetation alone.
Assuntos
Ecossistema , Poluentes do Solo , Bactérias , Biodegradação Ambiental , Expressão Gênica , Hidrocarbonetos , Micrococcaceae , SoloRESUMO
At present, the levels of cadmium (Cd) and zinc (Zn) in arable land are high and affect the growth and development of important food crops, including rice and maize. However, the application of silicon (Si) in contaminated areas increases the metal tolerance potential of these plants. This work aimed to study the variations in the distribution pattern of endogenous Si in various tissue regions in roots and leaves of rice and maize exposed to cadmium (Cd) and zinc (Zn) stresses. For these experiments, 45 day-old rice (var. Varsha) and maize (var. CoHM6) seedlings were treated with 1.95 g Zn and 0.45 g Cd kg-1 soil. Under Cd stress, the distribution of Si was high in the cortical region of the root, but under Zn stress, the highest Si deposition was found in the endodermis. In leaves, Si deposition was high in both the mesodermis and stelar regions of Cd-treated plants but more Si was deposited in the mesodermis tissue of Zn-treated plants. Heavy metal (Cd and Zn) accumulation and Si deposition showed a strong negative correlation in the roots of rice and maize plants. Complexation with metal ions and redistribution of Si were considered the major mechanisms in Si-mediated mitigation of Cd and Zn stress. Cd- and Zn-induced anatomical changes, such as endodermal thickening, deposits in the xylary elements and aerenchyma formation in the roots of rice and maize, were also associated with the Si distribution.
Assuntos
Oryza , Poluentes do Solo , Cádmio/toxicidade , Folhas de Planta/química , Raízes de Plantas/química , Silício/farmacologia , Poluentes do Solo/toxicidade , Zea mays , Zinco/toxicidadeRESUMO
Thiamin, an important member of the vitamin B family, is believed to play a significant role in mitigating environmental stresses including drought stress. In turnip, drought stress causes a reduced growth, biomass yield, pigment content, total phenolics and ascorbic acid (AsA), particularly at 50% field capacity (F.C.) in the two cultivars (cv) studied. However, a significant enhancement was observed in the contents of leaf proline, glycinebetaine (GB), malondialdehyde (MDA), hydrogen peroxide (H2 O2 ) and the activities of catalase (CAT) and superoxide dismutase (SOD) as well as root proline, GB, total phenolics, AsA, H2 O2 , MDA and the activities of peroxidase (POD) and SOD. However, foliar-applied thiamin significantly improved (particularly 100 mM) all the growth attributes, photosynthetic pigments, leaf and root osmoprotectants (GB and proline), AsA, total phenolics and the activities of enzymatic antioxidants such as SOD and POD as well as root CAT in both turnip cultivars under drought stress conditions. Foliar application of thiamin was effective in decreasing the leaf and root H2 O2 and MDA content in both cultivars particularly at 50% F.C. Thiamin-induced growth of both turnip cultivars, particularly of cv. Purple Top, was found to be associated with increased photosynthetic pigments, proline and GB contents and antioxidant capacity, but reduced levels of reactive oxygen species (ROS) under water deficit conditions. So, it is suggested that exogenous application of thiamin can be effective in improving drought tolerance of plants.
Assuntos
Brassica napus , Brassica rapa , Antioxidantes , Secas , Folhas de Planta , TiaminaRESUMO
Soil salinity is one of the major environmental stresses faced by the plants. Sodium chloride is the most important salt responsible for inducing salt stress by disrupting the osmotic potential. Due to various innate mechanisms, plants adapt to the sodic niche around them. Genes and transcription factors regulating ion transport and exclusion such as salt overly sensitive (SOS), Na+ /H+ exchangers (NHXs), high sodium affinity transporter (HKT) and plasma membrane protein (PMP) are activated during salinity stress and help in alleviating cells of ion toxicity. For salt tolerance in plants signal transduction and gene expression is regulated via transcription factors such as NAM (no apical meristem), ATAF (Arabidopsis transcription activation factor), CUC (cup-shaped cotyledon), Apetala 2/ethylene responsive factor (AP2/ERF), W-box binding factor (WRKY) and basic leucine zipper domain (bZIP). Cross-talk between all these transcription factors and genes aid in developing the tolerance mechanisms adopted by plants against salt stress. These genes and transcription factors regulate the movement of ions out of the cells by opening various membrane ion channels. Mutants or knockouts of all these genes are known to be less salt-tolerant compared to wild-types. Using novel molecular techniques such as analysis of genome, transcriptome, ionome and metabolome of a plant, can help in expanding the understanding of salt tolerance mechanism in plants. In this review, we discuss the genes responsible for imparting salt tolerance under salinity stress through transport dynamics of ion balance and need to integrate high-throughput molecular biology techniques to delineate the issue.
Assuntos
Arabidopsis , Tolerância ao Sal , Arabidopsis/genética , Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas , Homeostase , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Salinidade , Tolerância ao Sal/genética , Estresse FisiológicoRESUMO
This study was carried out to assess the influence of trehalose, a non-reducing disaccharide involved in improving plant stress tolerance, on two cultivars (Hysun 33 and FH 598) of sunflower (Helianthus annuus L.) grown under control and drought stress conditions. At pre-flowering stage, varying concentrations (10, 20 and 30 mM) of trehalose were applied to the foliage. Drought stress significantly suppressed the plant growth, total soluble proteins, chlorophyll, achene yield per plant, oil percentage, organic contents, as well as oil palmitic and linoleic acids in both sunflower cultivars. External application of trehalose significantly reduced RMP (relative membrane permeability), and the accumulation of H2 O2 (hydrogen peroxide), while a considerable improvement was recorded in shoot fresh and shoot and root dry weights, total soluble proteins, glycinebetaine, AsA (ascorbic acid), total phenolics, achene yield per plant, oil contents, inorganic and organic contents, and the activities of catalase (CAT), superoxide dismutase (SOD) and peroxidase (POD) enzymes under water-limited regimes. The cultivar Hysun 33 was superior to the other cultivar in plant growth, RMP, glycinebetaine, proline, achene yield per plant, oil contents, and palmitic and linoleic acids. Overall, foliar-applied trehalose improved plant growth, oxidative defense system, yield and oil composition of sunflower under drought stress conditions.
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Helianthus , Antioxidantes , Catalase , Secas , Folhas de Planta , TrealoseRESUMO
Salinity is a potential abiotic stress and globally threatens crop productivity. However, the molecular mechanisms underlying salt stress tolerance with respect to cytoplasmic effect, gene expression, and metabolism pathway under salt stress have not yet been reported in isonuclear kenaf genotypes. To fill this knowledge gap, growth, physiological, biochemical, transcriptome, and cytoplasm changes in kenaf cytoplasmic male sterile (CMS) line (P3A) and its iso-nuclear maintainer line (P3B) under 200 mM sodium chloride (NaCl) stress and control conditions were analyzed. Salt stress significantly reduced leaf soluble protein, soluble sugars, proline, chlorophyll content, antioxidant enzymatic activity, and induced oxidative damage in terms of higher MDA contents in both genotypes. The reduction of these parameters resulted in a reduced plant growth compared with control. However, P3A was relatively more tolerant to salt stress than P3B. This tolerance of P3A was further confirmed by improved physio-biochemical traits under salt stress conditions. Transcriptome analysis showed that 4256 differentially expressed genes (DEGs) between the two genotypes under salt stress were identified. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that photosynthesis, photosynthesis antenna-protein, and plant hormone signal transduction pathways might be associated with the improved NaCl stress tolerance in P3A. Conclusively, P3A cytoplasmic male sterile could be a potential salt-tolerant material for future breeding program of kenaf and can be used for phytoremediation of salt-affected soils. These data provide a valuable resource on the cytoplasmic effect of salt-responsive genes in kenaf and salt stress tolerance in kenaf.
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Hibiscus , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Genótipo , Estresse Salino , Tolerância ao Sal/genética , Estresse Fisiológico , TranscriptomaRESUMO
The unpredictable precipitation and water deficit conditions in semiarid regions significantly reduce the yield of summer maize. The exogenous application of plant growth regulators can be used as a strategy to enhance plant stress tolerance and improve the growth and yield of maize under semiarid conditions. Here, we studied the protective role of melatonin application on maize yield using grain filling rate and hormonal crosstalk in maize grains. In the first field experiment, seeds were soaked with melatonin at a concentration of 0 (SM0 ), 25 (SM1 ), 50 (SM2 ), and 75 µM (SM3 ) µM. In contrast, in the second experiment, melatonin was applied on the foliage at the ninth leaf stage at a concentration of 0 (FM0 ), 25 (FM1 ), 50 (FM2 ), and 75 (FM3 ) µM. Our findings showed that melatonin treatments as seed soaking significantly increased single seed weight, seed filling rate in superior, medium and inferior seeds by regulating the hormone levels compared to foliar application. Application of melatonin significantly increased the zeatin+zeatin riboside (Z+ZR), indole-3-acetic acid (IAA), and gibberellic acid (GA) contents. However, it significantly inhibited the contents of abscisic acid (ABA) during the seed filling period. The content of Z+ZR, IAA, and GA was positively correlated with the maximum seed filling rate, seed weight, and mean filling rate in middle, superior and lower seeds, while the ABA was negatively correlated. The ABA content in inferior seeds was positively correlated with the maximum and mean seed filling rate. In semiarid regions, melatonin treatment of SM2 and FM2 significantly increased the dry matter per plant, 100-grain weight, seed filling rate, IAA, Z+ZR, GA contents, ear characteristics, and maize yield.