Assessment of awake bruxism and oral mucosa indentation in adolescents.

BACKGROUND: Oral mucosa indentations can be signs of awake bruxism (AB) in adults, but this association has not yet been verified in adolescents. OBJECTIVES: To evaluate the frequency of AB in adolescents and determine whether there is an association between AB and oral mucosa indentation. METHODS: This study enrolled 66 high school students, mean age of 16.9 (±0.54) years. Clinical inspection was performed to assess the presence or absence of tongue, cheek and lip mucosa indentation. AB was assessed by the Ecological Momentary Assessment method using the WhatsApp mobile app. Messages were sent 15 times a day, 7 days, between 8:00 AM and 7:00 PM at random times to choose one of the five oral behaviours: teeth contact, teeth clenching, teeth grinding, mandible bracing and relaxed jaw muscles. The non-parametric Mann-Whitney U-test for independent samples, Friedman test for paired samples, Friedman pairwise multiple comparisons non-parametric test, Pearson's chi-squared tests, and z-test of comparisons between two proportions were performed (p < .05). RESULTS: During the week the frequency of AB behaviours was 56.20%, teeth contact was the most frequent (37.68% ± 22.26%), significantly more frequent than other AB behaviours; there was a greater frequency of cheek indentation (27.27%) and no difference between genders in oral behaviours and indentations (p > .05). A higher frequency of AB behaviours was observed in individuals with a greater frequency of cheek indentation (p < .05). CONCLUSIONS: Teeth contact and cheek indentation were the most frequent conditions among adolescents and AB behaviours are associated with this indentation.

The apoptotic effect of midazolam on the salivary gland of rats and the reversal effect by pilocarpine.

To evaluate the apoptosis in parotid glands of rats treated with midazolam associated or not with pilocarpine, 60 Wistar rats were assigned to 6 groups: control groups received saline solution for 30 days (S30) and 60 days (S60) and the other groups received pilocarpine for 60 days (P60), midazolam for 30 days (M30), midazolam for 30 days and 30 days of saline (M30 + S30), and finally midazolam for 30 days and 30 days of midazolam and pilocarpine (M30 + MP30). Histological sections were subjected to the TdT-mediated dUTP-biotin nick and labeling technique. The number of positive and negative cells was quantified, calculating the apoptotic index. ANOVA at 2 criteria and Tukey's test were used. A greater apoptotic index was observed in the M30 (52.79 ± 9.01) and M30 + S30 (62.43 ± 8.52) groups when compared with the S30 (37.94 ± 5.94) and S60 (31.85 ± 9.18) groups, respectively (p < 0.05). There was no difference between M30 + MP30 (30.98 ± 6.19) and S60 (31.85 ± 9.18) groups regarding apoptotic index. Chronic administration of midazolam has been shown to increase the number of apoptotic cells in the parotid glands of rats. However, pilocarpine inhibited this effect, thus inhibiting the apoptosis.

FasL expression in articular discs of human temporomandibular joint and association with osteoarthrosis.

BACKGROUND: Apoptosis is a programme of cell death which does not induce an inflammatory response. Recent previous research has suggested a correlation between temporomandibular internal derangement and apoptosis. Fas ligand (FasL) is an apoptosis-inducing factor, known to trigger apoptosis through distinct signal pathways. This study aims to examine, by immunohistochemistry, the expression of FasL in temporomandibular joint (TMJ) articular discs of patients with anterior disc displacement with reduction (ADDwR) and without reduction (ADDwoR) in patients with and without osteoarthrosis (OA). METHODS: Forty-two (n = 42) TMJ articular discs were divided into two cut-offs: (i) 8 control, 17 ADDwR, 17 ADDwoR, and (ii) without OA (n = 25) and with OA (n = 17). The area of immunostaining was compared statistically between groups (P < 0.05). RESULTS: Statistically significant differences were found in the expression of FasL in TMJ discs between the three groups (P = 0.001). ADDwR presented significant higher FasL expression when compared with ADDwoR (P < 0.001). Significant higher FasL expression was observed in the group without OA (P = 0.001). All patients without OA presented ADDwR, while all the patients with OA presented ADDwoR. CONCLUSION: A higher area of in situ immunostaining of FasL was found in temporomandibular discs with reduction, which is the less severe condition. Moreover, a reduced expression of FasL in the discs of patients with osteoarthrosis was found, suggesting that some aspects of apoptosis might underlie the progression of TMJ disorders.

Auricular acupuncture in TMD - A sham-controlled, randomized, clinical trial.

This study evaluated the effect of auricular acupuncture (AA) on physical (PA) and emotional (EA) aspects of patients with temporomandibular disorders (TMDs) and compared the effect of AA with those of Sham and occlusal splints (OS). In accordance with the CONSORT guidelines, patients with TMDs were evaluated by axes I and II of the RDC-TMD. The patients were allocated among three groups: OS, Sham and AA; and followed for 8 weeks. Both intragroup and intergroup evaluation for quantitative variables were analyzed with ANOVA, one-way, followed by the Tukey test. Qualitative variables were analyzed with the Kruskal-Wallis, and Dunn tests (intergroup evaluation) and for intragroup analyses, the Friedman and Wilcoxon tests were applied. At the end of the experiment, the chi-squared test was applied to compare the three groups with respect to the number of variables that had shown improved scores. The adopted level of significance for all tests was α = 5%. Intragroup analysis showed that, in the Sham group, no improvement in performance was seen in either EA and significant was improvements in 5 variables for 9 for PA, while in the AA group, significant improvements were observed in 2 variables for EA and in 9 for PA. In the OS group at the end of this study, significant improvements were observed in 8 and 8 variables for EA and PA, respectively. The analysis of therapies used revealed that the number of variables exhibiting significantly improved outcomes after treatment was similar between the AA and OS groups, whereas the sham group showed statistically significant differences when compared with the other two groups. In conclusion, this study demonstrated that AA applied at a specific TMJ ear point was able to bring about improvements in patients with TMDs, with outcomes similar to those in patients treated with an OS. CLINICAL TRIAL REGISTRATION: RBR-69ynnw (Brazilian Registry of Clinical Trials).

Fresh-frozen bone allografts in maxillary ridge augmentation: histologic analysis.

Bone allograft has become an alternative to autogenous bone due to its decreased operative trauma and the almost unlimited supply of reconstructive material. The aim of the present study was to histologically evaluate the suitability of fresh-frozen bone graft (test group) used in maxillary ridge augmentation, comparing it to autogenous bone (native maxilla: control group). During the re-entry procedures, 9 months after the fresh-frozen allogeneic bone blocks were placed in the atrophic maxillary ridges, bone cores were removed with a trephine bur from test and control treatments in the same patient. Routine histologic processing using hematoxylin and eosin and Picrosirius staining was performed. Mature and immature collagen area and density analysis were carried out for both groups under polarization. The results of Student's t test for paired samples (P > .05) showed no statistically significant difference in mature and immature collagen area or density percentage between test and control groups. Histologically similar bone formation patterns were observed in both groups. We concluded that fresh-frozen bone allograft is a biologically acceptable alternative for augmentation of the deficient alveolar ridge, showing a similar collagen pattern to that of autogenous bone.

Histochemical analysis of collagen fibers in giant cell fibroma and inflammatory fibrous hyperplasia.

OBJECTIVE: The aim was to investigate collagen fibers in giant cell fibroma, inflammatory fibrous hyperplasia, and oral normal mucosa. MATERIALS AND METHODS: Sixty-six cases were stained with picrosirius red. The slides were observed under polarization, followed by the measurement of the area and the percentage of the type I and type III collagens. The age and gender were obtained from the clinical records. RESULTS: No differences could be observed in both the area and percentage of the type I and type III collagens within the categories of lesions and normal mucosa. In the giant cells fibroma, a greater area and percentage of type I collagen could be identified in individuals of less than 41.5 years (p<0.05). CONCLUSION: The distribution of type I and type III collagen fibers in the studied lesions followed a similar pattern to that observed in the normal mucosa, indicating a normal collagen maturation process of type III to I. The study supports that multinucleated and stellate cells of the giant cell fibroma appear to be functional within collagen types III and I turnover. The greater amount of type I collagen identified in giant cell fibroma in individuals of less than 41.5 years reinforce the neoplastic nature of lesion.

MMP-1 and MMP-8 expression in giant-cell fibroma and inflammatory fibrous hyperplasia.

The aim of this study is to compare the immunoexpression of metalloproteinases 1 and 8 in giant-cell fibroma, inflammatory fibrous hyperplasia and normal mucosa. Twenty-two cases of giant-cell fibroma, inflammatory fibrous hyperplasia and oral mucosa (control) each were subjected to immunohistochemistry using anti-metalloproteinase-1 and anti-metalloproteinase-8 antibodies. Eight images of each case were captured and analysed through the a) application of a count grid to count the number of positive neutrophils, macrophages, lymphocytes, plasma cells, fibroblasts and blood vessels to obtain the percentage of staining and b) semi-automated segmentation quantifying the stained area in square micrometres. Statistical tests included ANOVA Two-way, Kruskal Wallis and Games-Howell, with a significance level of 5%. An increased percentage of metalloproteinase-1-immunopositive blood vessels were observed in giant-cell fibroma (26.6±22.4; p=0.02) and inflammatory fibrous hyperplasia (34.3±31.5; p=0.01) compared with the control group (19.6±9.2). No significant differences in inflammatory cells, fibroblasts and total area of metalloproteinase-1 and -8 were noted among the three groups. Metalloproteinase-1 apparently acts within the pathogenesis of giant-cell fibroma and inflammatory fibrous hyperplasia.

Evaluation of the bone anatomy of the anterior region of the mandible using cone beam computed tomography / Avaliação da anatomia da região anterior da mandíbula por meio de tomografia de feixe cônico

Background: Hemorrhages, mouth floor edema and tongue elevation are complications related to surgical procedures in the anterior region of the mandible. Objective: The objectives of this study were to evaluate the presence and location of the lingual foramen in the anterior region of the mandible and to evaluate mandibular morphology using cone beam computerized tomography (CBCT). Material and method: The mandible's morphology and the location, diameter and height of the lingual foramina were analyzed using the midline and the mental foramen as references, in 278 CBCT. Result: 88% of the sample had a midline lingual foramen, totaling 408 foramina, with a mean diameter of 0.93 mm. Foramina in the lingual region between the midline and mental foramina were detected in 75% of the sample, with a mean diameter of 0.807 mm. There was no positive correlation between the presence of lingual foramina in the lateral or in the midline regions (r = -0.149; p = 0.013). In the midline region, the type I mandibular shape was predominant (96%), and type III was predominant in the lateral regions. Conclusion: Considering the prevalence of these structures and their clinical relevance in potential surgical complications, it is important to carefully analyze the anterior region of the mandible during surgical planning.

Introdução: Hemorragias, edema no assoalho bucal e elevação da língua são complicações relacionadas a procedimentos cirúrgicos na região anterior da mandíbula. Objetivo: Os objetivos deste estudo foram avaliar a presença e localização do forame lingual na região anterior da mandíbula e avaliar a morfologia mandibular utilizando tomografia computadorizada com feixe de cone (CBCT). Material e método: A morfologia da mandíbula e a localização, diâmetro e altura do forame lingual foram analisados utilizando a medula e o forame mental como referências em 278 CBCT. Resultado: 88% da amostra tinha um forame lingual da linha média, totalizando 408 forames, com um diâmetro médio de 0,93 mm. Na região lingual entre a linha média e forames mentais foram detectados em 75% da amostra, com um diâmetro médio de 0,807 mm. Não houve correlação positiva entre a presença de forames lingual nas regiões lateral ou na região média (r = -0,149; p = 0,013). Na região da linha média, a forma mandibular do tipo I era predominante (96%) e o tipo III predominava nas regiões laterais. Conclusão: Considerando a prevalência dessas estruturas e sua relevância clínica em possíveis complicações cirúrgicas, é importante analisar cuidadosamente a região anterior da mandíbula durante o planejamento cirúrgico.

The effects of antidepressants and pilocarpine on rat parotid glands: an immunohistochemical study.

OBJECTIVES: To evaluate the effects of antidepressants and pilocarpine on the quantity of myoepithelial cells and on the proliferation index of the epithelial cells of rat parotid glands. INTRODUCTION: Hyposalivation, xerostomia, and alterations in saliva composition are important clinical side effects related to the use of antidepressants. METHODS: Ninety male Wistar rats were allocated to nine groups. The control groups received saline for 30 (group C30) or 60 days (group C60) or pilocarpine for 60 days (group Pilo). The experimental groups were administered fluoxetine (group F30) or venlafaxine for 30 days (group V30); fluoxetine (group FS60) or venlafaxine (group VS60) with saline for 60 days; or fluoxetine (group FP60) or venlafaxine (group VP60) with pilocarpine for 60 days. Parotid gland specimens were processed, and the immunohistochemical expression of calponin and proliferating cell nuclear anti-antigen on the myoepithelial and parenchymal cells, respectively, was evaluated. Analysis of variance (ANOVA), Tukey HSD and Games-Howell tests were applied to detect differences among groups (p<0.05). RESULTS: Compared with the controls, chronic exposure to antidepressants was associated with an increase in the number of positively stained cells for calponin. In addition, venlafaxine administration for 30 days was associated with an increase in the number of positively stained cells for proliferating cell nuclear anti-antigen. Fluoxetine and pilocarpine (group FP60) induced a significant decrease in the number of positively stained cells for calponin compared with all other groups. CONCLUSIONS: The number of positively stained cells for calponin increased after chronic administration of antidepressants. The proliferation index of the epithelial cells of rat parotid glands was not altered by the use of antidepressants for 60 days.

Avaliação da efetividade do laser de baixa potência na redução da dor pós-operatória em cirurgia de terceiros molares inferiores inclusos / Evaluation of the effectiveness of low power laser in reducing postoperative pain in surgery of mandibular third molar

Avaliar a efetividade do laser de baixa intensidade (LLLT) no controle da dor pós-operatória em cirurgia de terceiros molares inferiores inclusos, comparando-o com o lado oposto, que não foi exposto ao LLLT, no mesmo indivíduo.Metodologia: participaram do estudo 16 indivíduos, que apresentaram os dentes 38 e 48 inclusos, em posição similar, e que realizaram a remoção dos dentes no mesmo tempo cirúrgico. O laser foi aplicado imediatamente após a remoção do 48 (lado direito - LD), intra-alvéolo, de forma pontual e sobre a região da sutura, em varredura. O mesmo procedimento foi realizado no 38 (lado esquerdo - LE), com o aparelho desligado garantindo o desconhecimento do paciente sobre qual lado recebeu o laser. Todos foram medicados e orientados quanto aos cuidados pós-operatórios e receberam as escalas visuais análogas (VAS) que variavam de 0-10 cm, anotando a ausência ou presença da dor durante 5 dias do lado direito e esquerdo. Os dados foram tabulados e submetidos ao teste de Wilcoxon (p<0,05) comparando-se as médias da VAS de 1 a 5 dias. Resultados: no 3° dia LD e LE, houve diferença estatística significante (p= 0,0284) e do LD a dor foi menor. No 1° e 5° dia houve redução da dor em ambos os lados (LD:p=0,007; LE:p=0,001).Conclusão: dentro dos limites do presente estudo o laser de baixa intensidade mostrou efetividade no controle da dor pós-operatória...

The purpose of the present study was to analyze the effectiveness of LLLT in pain control after impacted third molar removal, comparing it with the opposite side, note exposed to LLLT in the same patient.Methodology: 16 patients who presented both impacted lower third molar in similar position and accepted to remove them in the same operative time were select to this study. LLLT was used immediately after lower right third molar (R3M), intra-socket and after wound closure. The same procedure was done in the opposite side (L3M) but this time the LLLT was not turned on, warrating thus the patient didn´t no which side had received the irradiation. All patients received visual analogue scale (VAS), where they were instructed to write down the presence or absence of pain in the first five postoperative days. Statistical analysis comprised Wilcoxon test (p<0.05), comparing the VAS average data in the first to the fifth postoperative day.Results: the third postoperative day resulted in a statistically significant (p=0.0284) reduction of pain. In the first and fifth day there was pain reduction in both sides (R3m: p=0.0071; L3M: p=0.0010).Conclusion: within the limits of this study LLLT was effective in postoperative pain control...

The effects of antidepressants and pilocarpine on rat parotid glands: an immunohistochemical study

OBJECTIVES: To evaluate the effects of antidepressants and pilocarpine on the quantity of myoepithelial cells and on the proliferation index of the epithelial cells of rat parotid glands. INTRODUCTION: Hyposalivation, xerostomia, and alterations in saliva composition are important clinical side effects related to the use of antidepressants. METHODS: Ninety male Wistar rats were allocated to nine groups. The control groups received saline for 30 (group C30) or 60 days (group C60) or pilocarpine for 60 days (group Pilo). The experimental groups were administered fluoxetine (group F30) or venlafaxine for 30 days (group V30); fluoxetine (group FS60) or venlafaxine (group VS60) with saline for 60 days; or fluoxetine (group FP60) or venlafaxine (group VP60) with pilocarpine for 60 days. Parotid gland specimens were processed, and the immunohistochemical expression of calponin and proliferating cell nuclear anti-antigen on the myoepithelial and parenchymal cells, respectively, was evaluated. Analysis of variance (ANOVA), Tukey HSD and Games-Howell tests were applied to detect differences among groups (p<0.05). RESULTS: Compared with the controls, chronic exposure to antidepressants was associated with an increase in the number of positively stained cells for calponin. In addition, venlafaxine administration for 30 days was associated with an increase in the number of positively stained cells for proliferating cell nuclear anti-antigen. Fluoxetine and pilocarpine (group FP60) induced a significant decrease in the number of positively stained cells for calponin compared with all other groups. CONCLUSIONS: The number of positively stained cells for calponin increased after chronic administration of antidepressants. The proliferation index of the epithelial cells of rat parotid glands was not altered by the use of antidepressants for 60 days.