RESUMO
OBJECTIVES: The aim of this study was to evaluate the ability of four calcium channel blockers (CCBs), namely verapamil, diltiazem, nicardipine (NIC) and nifedipine (NIF), to enhance the susceptibility of Candida glabrata strains to fluconazole (FLC). METHODS: Synergistic antifungal effects of the CCBs with FLC were examined by the chequerboard method, and fractional inhibitory concentration indices (FICIs) were determined. The time-kill curve method was used for the most promising combination to further evaluate the synergetic effects. RESULTS: NIC showed an additive effect with FLC against FLC-resistant and FLC-susceptible-dose-dependent strains (DSY 565 and CBS 138) known to express efflux pumps, but not against FLC-susceptible strains. NIF exhibited an additive effect with FLC both by the chequerboard method (0.5Assuntos
Bloqueadores dos Canais de Cálcio/farmacologia
, Candida glabrata/efeitos dos fármacos
, Farmacorresistência Fúngica/efeitos dos fármacos
, Fluconazol/farmacologia
, Bloqueadores dos Canais de Cálcio/química
, Diltiazem/farmacologia
, Relação Dose-Resposta a Droga
, Sinergismo Farmacológico
, Testes de Sensibilidade Microbiana
, Viabilidade Microbiana/efeitos dos fármacos
, Nicardipino/farmacologia
, Nifedipino/farmacologia
, Verapamil/farmacologia