An intronic single-nucleotide polymorphism (rs13217795) in FOXO3 is associated with asthma and allergic rhinitis: a case-case-control study.

BACKGROUND: Asthma and allergic rhinitis are respiratory diseases with a significant global burden. Forkhead box O3 (FOXO3) is a gene involved in the etiology of a number of respiratory diseases. The objective of this study is to assess the association of rs13217795, an intronic FOXO3 single-nucleotide polymorphism, with asthma and allergic rhinitis. METHODS: In this case-case-control genetic association study, genotyping was conducted using the PCR-RFLP method. Genotype-based associations were investigated under the general, recessive, and dominant models of disease penetrance using binomial logistic regression; and, allele-based associations were tested using Pearson's chi-squared test. RESULTS: The final study population consisted of 94 controls, 124 asthmatics, and 110 allergic rhinitis patients. The general and recessive models of disease penetrance were statistically significant for both case-control comparisons. Under the general model, the odds of the asthma phenotype were 1.46 (0.64 to 3.34) and 3.42 (1.37 to 8.57) times higher in heterozygotes and derived allele homozygotes, respectively, compared to ancestral allele homozygotes. The corresponding odds ratios for the allergic rhinitis phenotype were 1.05 (0.46 to 2.40) and 2.35 (0.96 to 5.73), respectively. The dominant model of disease penetrance was not statistically significant. The minor allele in all study groups was the ancestral allele, with a frequency of 0.49 in controls. There was no deviation from Hardy-Weinberg equilibrium in controls. Both case-control allele-based associations were statistically significant. CONCLUSIONS: Herein we present the first report of the association between rs13217795 and allergic rhinitis, and the first independent verification of the association between rs13217795 and asthma. Marker selection in future genetic association studies of asthma and allergic rhinitis should include functional polymorphisms in linkage disequilibrium with rs13217795.

Prevalence and Configuration of the Second Mesiobuccal Canal in the Permanent Maxillary First Molar in Jordanian Population Sample.

Introduction: Missing a root canal during endodontic treatment implicates the persistence of microbial infection within the root canal system. This study aims to evaluate the incidence and morphology of the second mesiobuccal canal in the maxillary first molars in the Jordanian population. Methods and Materials: Consecutive cone-beam computed tomography (CBCT) scans taken at Jordan University Hospital were assessed in this retrospective study. A total of 200 scans that were examined, 111 were included in this study. The scans that were included had to have a full view of the maxilla with at least one permanent maxillary first molar. The following data were collected: the presence of a second mesiobuccal canal, the configuration of the mesiobuccal canals, the status of the apical area and the mesiobuccal inter-orifice distance, if applicable. The prevalence and morphology of the second mesiobuccal canal was determined and its association with biological sex and right or left sidedness was measured using the Chi-Square test. Results: The prevalence of the second mesiobuccal canal in our sample of the Jordanian population was 87%, with the most common canal configuration being Vertucci type II. The average inter-orifice distance between first and second mesiobuccal canals was 1.9 ± 0.4 mm. Conclusion: This retrospective study is the first in Jordan to document the prevalence of the second mesiobuccal canal using CBCT, and it shows that the vast majority of teeth have a second mesiobuccal canal. The proper location and negotiation of this canal is of dire importance for endodontic therapy.