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1.
Osteoarthritis Cartilage ; 18(9): 1183-91, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20633683

RESUMO

OBJECTIVE: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-beta1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. METHODS: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200 ng of TGF-beta1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-beta1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. RESULTS: Complete filling of the defects with regenerated tissue was observed in both the TGF-beta1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-beta1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-beta1-pretreated rabbits, 121 microm (94-149), compared to controls, 74 microm (52-96), after 6 weeks. CONCLUSIONS: This study demonstrates that in situ pretreatment of periosteum with TGF-beta1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.


Assuntos
Cartilagem Articular/efeitos dos fármacos , Cartilagem Articular/lesões , Condrogênese/efeitos dos fármacos , Periósteo/efeitos dos fármacos , Periósteo/patologia , Engenharia Tecidual/métodos , Fator de Crescimento Transformador beta1/farmacologia , Animais , Cartilagem Articular/fisiopatologia , Condrogênese/fisiologia , Modelos Animais de Doenças , Coelhos , Transplante de Tecidos , Transplante Autólogo , Cicatrização/efeitos dos fármacos , Cicatrização/fisiologia
2.
Proc Inst Mech Eng H ; 223(2): 133-42, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19278191

RESUMO

While researchers have suggested that joint mobility would probably be affected by age and gender, research findings often present discrepancies. Little research has been performed on the factors which effect mobility of the trapeziometacarpal (TMC) joint. The purpose of this study was to address the effects of age and gender on the ranges of motion of the normal TMC joint. Eighty normal subjects divided into four age groups participated in this study. The TMC joint motions were recorded using an electromagnetic tracking system. In order to achieve a maximal range of TMC joint motion which was defined as the maximal workspace, each subject was asked to perform actively maximal circumduction, flexion-extension, and abduction-adduction of the TMC joint. Numerical and statistical methods were used to compute the TMC workspace and to detect significant differences. A workspace-to-length ratio was determined as an index to examine the effects of the age and gender on the joint mobility. The results demonstrated that age and gender had significant influences on the TMC workspace among the groups studied. The understanding of TMC joint mobility under different age and gender conditions is achieved through this study. The findings can be used to report clinical measures in the determination of the extent of impairment of osteoarthritis as well as the outcomes between pre- and post-surgical (or non-surgical) interventions.


Assuntos
Envelhecimento/fisiologia , Articulações dos Dedos/fisiologia , Ossos Metacarpais/fisiologia , Movimento/fisiologia , Amplitude de Movimento Articular/fisiologia , Polegar/fisiologia , Trapézio/fisiologia , Adolescente , Adulto , Fatores Etários , Idoso , Criança , Simulação por Computador , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Biológicos , Fatores Sexuais , Análise e Desempenho de Tarefas , Adulto Jovem
3.
Bone Joint Res ; 8(6): 232-245, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31346451

RESUMO

OBJECTIVES: Re-rupture is common after primary flexor tendon repair. Characterization of the biological changes in the ruptured tendon stumps would be helpful, not only to understand the biological responses to the failed tendon repair, but also to investigate if the tendon stumps could be used as a recycling biomaterial for tendon regeneration in the secondary grafting surgery. METHODS: A canine flexor tendon repair and failure model was used. Following six weeks of repair failure, the tendon stumps were analyzed and characterized as isolated tendon-derived stem cells (TDSCs). RESULTS: Failed-repair stump tissue showed cellular accumulation of crumpled and disoriented collagen fibres. Compared with normal tendon, stump tissue had significantly higher gene expression of collagens I and III, matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), and insulin-like growth factor (IGF). The stump TDSCs presented both mesenchymal stem and haematopoietic cell markers with significantly increased expression of CD34, CD44, and CD90 markers. Stump TDSCs exhibited similar migration but a lower proliferation rate, as well as similar osteogenic differentiation but a lower chondrogenic/adipogenic differentiation capability, compared with normal TDSCs. Stump TDSCs also showed increasing levels of SRY-box 2 (Sox2), octamer-binding transcription factor 4 (Oct4), tenomodulin (TNMD), and scleraxis (Scx) protein and gene expression. CONCLUSION: We found that a failed repair stump had increased cellularity that preserved both mesenchymal and haematopoietic stem cell characteristics, with higher collagen synthesis, MMP, and growth factor gene expression. This study provides evidence that tendon stump tissue has regenerative potential.Cite this article: C-C. Lu, T. Zhang, R. L. Reisdorf, P. C. Amadio, K-N. An, S. L. Moran, A. Gingery, C. Zhao. Biological analysis of flexor tendon repair-failure stump tissue: A potential recycling of tissue for tendon regeneration. Bone Joint Res 2019;8:232-245. DOI: 10.1302/2046-3758.86.BJR-2018-0239.R1.

4.
J Orthop Surg (Hong Kong) ; 16(3): 326-32, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19126900

RESUMO

PURPOSE: To evaluate the effect of prosthesis design on stress profile in the proximal femur after hip resurfacing. METHODS: The von Mises stress profile of the native femur was simulated and compared with that of resurfaced femurs using various prosthetic materials (titanium, cobalt-chrome, ceramic), stem lengths (normal, half, short, and no stem), and femoral head coverage (shell size) [260 degrees, 220 degrees, 180 degrees, and 140 degrees]. RESULTS: Hip resurfacing altered the stress profile of the cancellous (but not cortical) bone of the femoral neck. Maximal cortical stresses were observed at the posterior half of the medial femoral neck. The stress profile of the native femur was most similar to that of the resurfaced femur made of titanium, with a short or no stem and 260 degrees of femoral head coverage (shell size). CONCLUSION: Optimising prosthesis design by minimising biomechanical alterations seems a valid approach to achieving favourable long-term outcomes. Cadaveric and in vivo studies are needed to confirm the clinical relevance and feasibility.


Assuntos
Cabeça do Fêmur/fisiopatologia , Cabeça do Fêmur/cirurgia , Análise de Elementos Finitos , Prótese de Quadril , Desenho de Prótese , Estresse Mecânico , Artroplastia de Quadril/instrumentação , Análise de Falha de Equipamento , Humanos , Modelos Biológicos , Suporte de Carga/fisiologia
5.
Bone Joint Res ; 6(3): 179-185, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28360084

RESUMO

OBJECTIVES: The present study describes a novel technique for revitalising allogenic intrasynovial tendons by combining cell-based therapy and mechanical stimulation in an ex vivo canine model. METHODS: Specifically, canine flexor digitorum profundus tendons were used for this study and were divided into the following groups: (1) untreated, unprocessed normal tendon; (2) decellularised tendon; (3) bone marrow stromal cell (BMSC)-seeded tendon; and (4) BMSC-seeded and cyclically stretched tendon. Lateral slits were introduced on the tendon to facilitate cell seeding. Tendons from all four study groups were distracted by a servohydraulic testing machine. Tensile force and displacement data were continuously recorded at a sample rate of 20 Hz until 200 Newton of force was reached. Before testing, the cross-sectional dimensions of each tendon were measured with a digital caliper. Young's modulus was calculated from the slope of the linear region of the stress-strain curve. The BMSCs were labeled for histological and cell viability evaluation on the decellularized tendon scaffold under a confocal microscope. Gene expression levels of selected extracellular matrix tendon growth factor genes were measured. Results were reported as mean ± SD and data was analyzed with one-way ANOVAs followed by Tukey's post hoc multiple-comparison test. RESULTS: We observed no significant difference in cross-sectional area or in Young's modulus among the four study groups. In addition, histological sections showed that the BMSCs were aligned well and viable on the tendon slices after two-week culture in groups three and four. Expression levels of several extracellular matrix tendon growth factors, including collagen type I, collagen type III, and matrix metalloproteinase were significantly higher in group four than in group three (p < 0.05). CONCLUSION: Lateral slits introduced into de-cellularised tendon is a promising method of delivery of BMSCs without compromising cell viability and tendon mechanical properties. In addition, mechanical stimulation of a cell-seeded tendon can promote cell proliferation and enhance expression of collagen types I and III in vitro.Cite this article: J. H. Wu, A. R. Thoreson, A. Gingery, K. N. An, S. L. Moran, P. C. Amadio, C. Zhao. The revitalisation of flexor tendon allografts with bone marrow stromal cells and mechanical stimulation: An ex vivo model revitalising flexor tendon allografts. Bone Joint Res 2017;6:179-185. DOI: 10.1302/2046-3758.63.BJR-2016-0207.R1.

6.
J Orthop Res ; 24(3): 569-75, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16463363

RESUMO

The role of transforming growth factor beta (TGF-beta) in tendon healing is still not clearly established. TGF-beta affects gene expression primarily through the activation of the Smad signaling pathway. The first step in the Smad pathway is the expression of TGF-beta inducible early gene (TIEG). Recently, a TIEG knockout mouse has been developed. The purpose of this study was to examine the healing potential of flexor tendons in mice lacking the TIEG gene, and to further examine what role the TIEG pathway plays in flexor tendon repair. Twenty-two mice, consisting of 11 normal wild-type mice and 11 TIEG knockout mice, were euthanized at 8 to 12 weeks of age. The second through fifth FDL tendons of both hind feet were transected and repaired in zone 2. The repaired tendons were removed from the mice and placed into tissue culture. Tendons were then examined at days 3, 7, 14, 21, and 42 after surgery. Hematoxylin and eosin (HE) staining and immunohistochemical staining for TGF-beta, collagen type I, and collagen type III were performed. Reverse transcriptase polymerase chain reaction (RT-PCR) was performed to examine expression of TGF-beta1, beta2, beta3, and collagen type I and III. At 42 days after surgery, HE staining showed coaptation of lacerated tendon ends in both groups. Both groups showed healing of the lacerated tendon, but the chronologic expression pattern of TGF-beta was different between the knockout and normal tendons. TIEG deficient tendons had delayed expression of TGF-beta when compared with control tendons. The collagen mRNA expression pattern was similar with both groups, but the expression level was different, with TIEG knockout tendons having a lower expression of collagen type I mRNA (p < 0.001). TGF-beta is thought to play a major role in tendon healing. Healing of tendons in the TIEG knockout mouse suggests the possibility of tendon healing in the absence of the Smad pathway. The knockout mouse model described in the present study provides a novel means for further understanding of the tendon healing process through isolated deletion of specific growth factors.


Assuntos
Traumatismos dos Tendões , Traumatismos dos Tendões/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Cicatrização/fisiologia , Animais , Colágeno Tipo I/metabolismo , Colágeno Tipo III/metabolismo , Proteínas de Ligação a DNA/deficiência , Proteínas de Ligação a DNA/genética , Técnica Indireta de Fluorescência para Anticorpo , Expressão Gênica , Regulação da Expressão Gênica , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Traumatismos dos Tendões/patologia , Tendões/patologia , Fatores de Transcrição/deficiência , Fatores de Transcrição/genética , Fator de Crescimento Transformador beta/genética
7.
Clin Biomech (Bristol, Avon) ; 21(6): 554-9, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16530305

RESUMO

Background. The treatment of radial head fractures that are not amenable to an open reduction and internal fixation, remains to be a difficult issue. A potential problem with prosthetic replacement of the radial head is the shape of current radial head prostheses. The purpose of this study was to determine the effect of the shape of the radial head on kinematics and load transfer of the elbow. Methods. Kinematics of the elbow and radiocapitellar force transmission were measured in 6 fresh frozen upper extremities. The effect of radial head shape was tested by rotating the head 90 degrees , with a custom-made 'native' radial head prosthesis. 3-D spatial orientation of the ulna showed an average difference in ulnohumeral laxity, between the nominal and 90 degrees conditions, of 0.1 degrees throughout the arc of motion with neutral forearm rotation (maximum: 2 degrees ). Findings. We found an average difference in ulnar axial rotation, of 0.1 degrees (maximum: 1.9 degrees ). No differences showed statistical significance. Radiohumeral joint force was measured and maximally showed a 32 times increase of force in the altered shape conditions. Interpretation. Our results show that the kinematics of the elbow was not affected by altering the shape of the radial head, but it did adversely affect the forces in the radiohumeral joint. This could possibly generate degenerative changes in the elbow.


Assuntos
Articulação do Cotovelo/anatomia & histologia , Articulação do Cotovelo/fisiologia , Movimento/fisiologia , Rádio (Anatomia)/anatomia & histologia , Rádio (Anatomia)/fisiologia , Amplitude de Movimento Articular/fisiologia , Fenômenos Biomecânicos/métodos , Cadáver , Humanos , Úmero/anatomia & histologia , Úmero/fisiologia , Técnicas In Vitro , Rotação , Estresse Mecânico , Ulna/anatomia & histologia , Ulna/fisiologia
8.
Biorheology ; 43(3,4): 337-45, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16912406

RESUMO

Knowledge of the adaptation of the soft tissue to mechanical factors and biomolecules would be essential to better understand the mechanism of tendon injury and to improve the outcome of tendon repair. The responses to these factors could be different for the distinct types of cells in the tendon: cells from the tendon sheath, fibroblasts from the epitenon surface, or fibroblasts from the internal endotenon. In this study, we examined the mechanical and histological characteristics of the rate of contraction of the collagen gel seeded with epitenon and endotenon fibroblasts. The rate of contraction and the mechanical property of the contracted construct depend on the gel concentration and also the treatment of TGF-beta1.


Assuntos
Colágeno/metabolismo , Mecanotransdução Celular/fisiologia , Tendões/citologia , Animais , Células Cultivadas , Cães , Relação Dose-Resposta a Droga , Fibroblastos/efeitos dos fármacos , Fibroblastos/fisiologia , Géis , Estresse Mecânico , Tendões/efeitos dos fármacos , Tendões/metabolismo , Fator de Crescimento Transformador beta/farmacologia , Fator de Crescimento Transformador beta1
9.
Bone Joint Res ; 5(10): 453-460, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27729312

RESUMO

OBJECTIVES: The bony shoulder stability ratio (BSSR) allows for quantification of the bony stabilisers in vivo. We aimed to biomechanically validate the BSSR, determine whether joint incongruence affects the stability ratio (SR) of a shoulder model, and determine the correct parameters (glenoid concavity versus humeral head radius) for calculation of the BSSR in vivo. METHODS: Four polyethylene balls (radii: 19.1 mm to 38.1 mm) were used to mould four fitting sockets in four different depths (3.2 mm to 19.1mm). The SR was measured in biomechanical congruent and incongruent experimental series. The experimental SR of a congruent system was compared with the calculated SR based on the BSSR approach. Differences in SR between congruent and incongruent experimental conditions were quantified. Finally, the experimental SR was compared with either calculated SR based on the socket concavity or plastic ball radius. RESULTS: The experimental SR is comparable with the calculated SR (mean difference 10%, sd 8%; relative values). The experimental incongruence study observed almost no differences (2%, sd 2%). The calculated SR on the basis of the socket concavity radius is superior in predicting the experimental SR (mean difference 10%, sd 9%) compared with the calculated SR based on the plastic ball radius (mean difference 42%, sd 55%). CONCLUSION: The present biomechanical investigation confirmed the validity of the BSSR. Incongruence has no significant effect on the SR of a shoulder model. In the event of an incongruent system, the calculation of the BSSR on the basis of the glenoid concavity radius is recommended.Cite this article: L. Ernstbrunner, J-D. Werthel, T. Hatta, A. R. Thoreson, H. Resch, K-N. An, P. Moroder. Biomechanical analysis of the effect of congruence, depth and radius on the stability ratio of a simplistic 'ball-and-socket' joint model. Bone Joint Res 2016;5:453-460. DOI: 10.1302/2046-3758.510.BJR-2016-0078.R1.

10.
J Bone Joint Surg Am ; 87(9): 2019-27, 2005 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16140818

RESUMO

BACKGROUND: Many unlinked total elbow replacement designs with radically differing articular geometries exist, suggesting that there is no consensus regarding an optimal design. A feature inherent to the articular design is the intrinsic constraint afforded to the joint by the implant. Our aim was to compare the intrinsic constraints of unlinked implants with that of the normal ulnotrochlear joint. METHODS: We tested twelve cadaveric ulnotrochlear joints with a custom-made multiple-axis materials testing machine. With compressive loads ranging from 10 to 100 N, the joints were moved in either valgus or varus directions at 90 degrees of flexion. The ulnotrochlear components from a single example of five medium-sized unlinked elbow replacements (Ewald, Kudo, Pritchard ERS, Sorbie-Questor, and Souter-Strathclyde) were also tested. The recorded measurements included the torques and forces, angular displacement, and axial displacement of the humerus relative to the ulna. RESULTS: In general, the peak torque and the constraint ratio significantly increased with increasing compressive load for the implants as well as for the normal elbow. In valgus displacement, the Souter-Strathclyde implant had the highest and the Sorbie-Questor had the smallest peak torque and the Souter-Strathclyde had the highest and the Ewald had the smallest constraint ratio. In varus displacement, the Kudo had the highest and the Ewald had the smallest peak torque and constraint ratio. CONCLUSIONS: The constraint ratio is a characteristic that is useful for describing elbow joint behavior and for comparing the behavior of implants with that of the human elbow. Of the unlinked implants tested, the Souter-Strathclyde and Kudo prostheses most closely approximated the behavior of the human elbow joint. Implants that resemble the human elbow in appearance do not replicate normal behavior consistently, whereas other implants that do not resemble the human elbow closely do not deviate markedly from human behavior. Thus, much basic information about elbow form and function is needed to improve the performance of total elbow prostheses.


Assuntos
Artroplastia de Substituição , Articulação do Cotovelo , Prótese Articular , Idoso , Idoso de 80 Anos ou mais , Fenômenos Biomecânicos , Cadáver , Feminino , Humanos , Masculino , Teste de Materiais , Pessoa de Meia-Idade , Desenho de Prótese
11.
J Biomech ; 38(3): 503-7, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15652548

RESUMO

Adhesion between the tendon and tendon sheath after primary flexor tendon repair is seen frequently, and postoperative finger function is occasionally unsatisfactory. A reduction of the friction may facilitate tendon mobilization, which in turn may reduce the risk of the adhesion and restriction of range of motion. We considered the possibility of utilizing the hyaluronic acid (HA) as a lubricant. To evaluate the effect of HA, the gliding resistance between the canine flexor digitorum profundus tendon repaired by a modified Kessler suture technique with running epitendinous suture and the annular pulley located on the proximal phalanx (corresponding to the A2 pulley in humans) was evaluated and compared before and after administration of HA. The HA solution measurement groups were identified as follows; intact tendon as a control; repaired tendon; tendon soaked in 0.1, 1, and 10 mg/ml HA. The resistance increased after repairing, then it decreased after soaking in 10 mg/ml HA solution. The results of this study revealed that HA diminishes the excursion resistance after flexor tendon repair. We believe that some style of administration of the HA might reduce the excursion resistance and prevent adhesion until the synovial surface is fully developed.


Assuntos
Ácido Hialurônico/farmacologia , Traumatismos dos Tendões/patologia , Tendões/patologia , Aderências Teciduais/prevenção & controle , Animais , Cães , Relação Dose-Resposta a Droga , Membro Anterior , Fricção , Ácido Hialurônico/uso terapêutico , Lubrificação , Modelos Animais
12.
Med Eng Phys ; 27(4): 336-42, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15823475

RESUMO

Comminuted radial head fractures can be treated with a radial head implant. The effects of lengthening (2.5 mm, 5 mm) and shortening (2.5 mm, 5 mm) of the radial neck, were compared to the nominal length in six human upper extremity cadavers. Total varus-valgus laxity and ulnar rotation were recorded. We hypothesized that restoring the exact length of the radius is important to maintain normal kinematics in the elbow joint. Lengthening or shortening of more than 2.5 mm significantly changed elbow kinematics. Lengthening caused a significant decrease (p < 0.001) in varus-valgus laxity, with the ulna tracking in varus and external rotation. Shortening caused a significant increase in varus-valgus laxity (p < 0.001) and ulnar rotation (p < 0.001), with the ulna tracking in valgus and internal rotation. Our study suggests that a restoration of radial length is important and that axial understuffing or overstuffing the radiohumeral joint by 2.5 mm or more, will alter elbow kinematics.


Assuntos
Articulação do Cotovelo/fisiopatologia , Articulação do Cotovelo/cirurgia , Análise de Falha de Equipamento/métodos , Prótese Articular , Ajuste de Prótese/métodos , Fraturas do Rádio/fisiopatologia , Fraturas do Rádio/cirurgia , Idoso , Idoso de 80 Anos ou mais , Fenômenos Biomecânicos/métodos , Cadáver , Feminino , Humanos , Técnicas In Vitro , Masculino , Pessoa de Meia-Idade , Desenho de Prótese , Amplitude de Movimento Articular , Resultado do Tratamento
13.
Clin Biomech (Bristol, Avon) ; 20(5): 491-7, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15836936

RESUMO

BACKGROUND: An experiment has recently been conducted to evaluate and compare the differences in tendon excursions between the flexor digitorum profundus and superficialis using three mobilization techniques. No previous studies deal with the total joint excursions with constant tendon length. The purpose of this study was to investigate the coordinated motion between the finger and wrist joints resulting from passive tension of the muscles while performing synergistic wrist motion. METHODS: The relative joint positions of the hand and wrist were measured using a three-dimensional motion analysis system with external retroreflective markers 2 mm in diameter placed on the dorsal surface of the hand. Fifty normal subjects, with a 1:1 gender ration, ranging in age from 20 to 40 years, and with no previous history of upper extremity injury, were recruited for the experiment. FINDINGS: The relationships of synergistic motion between the wrist and finger joints due to passive tension in the muscles were approximately linear. The ranges of wrist motion averaged 60 degrees extension and 60 degrees flexion. Moving the wrist from flexion into extension induced synergistic finger joint motion as follows: the distal interphalangeal joint angles changed from an average of 12 degrees of flexion to 31 degrees; proximal-interphalangeal joint angles changed from 19 degrees to 70 degrees; and metacarpal phalangeal joints changed from 27 degrees to 63 degrees of flexion. INTERPRETATION: The relationships of synergistic motion between the wrist and finger joints were systematically documented. Such a relationship could be considered in optimizing the design of dynamic splints used for rehabilitation in post-surgical tendon repair, as well as providing useful information about potential diagnoses of problems with the integrity of the flexor and extensor mechanisms.


Assuntos
Articulações dos Dedos/fisiologia , Modelos Biológicos , Movimento/fisiologia , Equilíbrio Postural/fisiologia , Amplitude de Movimento Articular/fisiologia , Articulação do Punho/fisiologia , Adulto , Simulação por Computador , Feminino , Humanos , Masculino
14.
J Hand Surg Br ; 30(6): 626-32, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16131467

RESUMO

Flexor tendon repair remains one of the more difficult technical tasks facing the hand surgeon. A good repair must be both strong and able to glide smoothly through the tendon sheath. The purpose of this study is to present a model that allows surgeons to improve their technique of flexor tendon repair by receiving feedback on these important biomechanical parameters. The set-up requires testing equipment found in most biomechanical laboratories and should be available in many academic medical centres. Preliminary data suggest that receiving feedback about the strength and smoothness of a flexor tendon repair may be a very useful tool in helping surgeons improve the overall quality of their tendon repair technique.


Assuntos
Cirurgia Geral/educação , Ensino/métodos , Traumatismos dos Tendões , Tendões/cirurgia , Humanos , Conhecimento Psicológico de Resultados , Lacerações/cirurgia , Suturas , Tendões/fisiopatologia , Resistência à Tração , Transdutores
15.
Neurology ; 55(8): 1135-43, 2000 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-11071491

RESUMO

BACKGROUND: Immunoglobulin (Ig) administration induces remyelination in the Theiler's virus model of MS. METHODS: A randomized, double-blinded, placebo-controlled trial of IV immunoglobulin (IVIg) was performed in patients with MS who had persistent muscle weakness that had been stable for between 4 and 18 months to determine whether this would improve muscle strength (primary outcome: isometric muscle strength). Patients received either IVIg (0.4 g/kg) or placebo daily for 5 days, then single infusions every 2 weeks for 3 months (total, 11 infusions). Muscle groups identified by clinical measures to have unchanging significant weakness were the major targets for therapeutic response (targeted neurologic deficit [TND]). RESULTS: IVIg was well tolerated. An interim analysis after 67 patients were enrolled indicated no difference in the degree of change in strength between treatment groups in either the TND or non-TND muscle groups at 6 months, and the trial was terminated. There was no apparent benefit in relapse behavior or impairment measures during the 6-month observation period. Nor was there apparent benefit in either patients who remained clinically stable or in those with evidence of disease activity. Patients with active MS during the trial worsened in both TND and non-TND muscle groups. This worsening was seen regardless of whether the clinical manifestations of disease activity involved the TND muscle groups. CONCLUSIONS: IVIg does not reverse established weakness in MS. Measurements of isometric muscle strength were reliable (reproducible) indices of strength and may be sensitive, objective methods to document functional changes in impairment in future MS trials.


Assuntos
Imunoglobulinas Intravenosas/uso terapêutico , Distrofias Musculares/tratamento farmacológico , Adulto , Método Duplo-Cego , Feminino , Humanos , Imunoglobulinas Intravenosas/administração & dosagem , Contração Isométrica/efeitos dos fármacos , Contração Isométrica/fisiologia , Masculino , Pessoa de Meia-Idade , Músculos/efeitos dos fármacos , Músculos/fisiopatologia , Distrofias Musculares/fisiopatologia , Prognóstico
16.
Neuroscience ; 40(3): 793-804, 1991.
Artigo em Inglês | MEDLINE | ID: mdl-2062442

RESUMO

The successful application of a physiological model of the musculoskeletal system capable of accounting for nonequilibrium dynamic loading and predicting individual muscle forces in the knee is presented. The model incorporates rigid-body mechanics and musculoskeletal physiology. Unknown muscle and joint contact forces outnumber the equilibrium equations resulting in an indeterminant problem. Mathematical optimization is utilized to resolve the indeterminacy. The model is used to estimate individual muscle forces during isokinetic exercise. Five subjects were tested at speeds of 60 degrees/s and 180 degrees/s. A newly proposed optimal criterion, minimizing muscular activation, results in muscle force predictions which have significantly higher correlations with myoelectric activity than other linear and nonlinear optimal criteria. The results demonstrate that properly constrained linear programming methods do not limit the number of active muscles and allow for uniform recruitment of the active muscles.


Assuntos
Articulação do Joelho/fisiologia , Modelos Biológicos , Contração Muscular , Adulto , Fenômenos Biomecânicos , Eletromiografia , Humanos , Masculino , Estresse Mecânico
17.
Mayo Clin Proc ; 73(6): 508-15, 1998 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9621856

RESUMO

OBJECTIVE: To study the superior-inferior stabilizing functions of the coracohumeral ligament (CHL) and the rotator interval capsule (RIC) with use of a material testing machine. MATERIAL AND METHODS: The axial translations of the humerus with the superior-inferior translation force of 30 N applied were recorded under the following joint capsule conditions: (1) intact, (2) vented, (3) the CHL sectioned, and (4) the RIC incised in six cadaver shoulders. The order of sectioning was changed for conditions 3 and 4 in six other cadaver shoulders. RESULTS: With the arm in internal and neutral rotations, venting the capsule significantly increased the superior-inferior translation, which was unaffected by further sectioning of the CHL and the RIC. With the arm in external rotation, only the CHL contributed significantly to inferior stability, whereas both this ligament and the RIC contributed to superior stability to a lesser degree. CONCLUSION: The CHL is a stabilizer in superior inferior directions with the arm in external rotation, and the intra-articular pressure that is maintained by the intact RIC is a stabilizer in superior-inferior directions with the arm in internal and neutral rotations. These findings may provide a scientific background to support closure of the interval space to stabilize the shoulder and may explain part of the superior instability observed in shoulders with rotator cuff tears.


Assuntos
Instabilidade Articular/fisiopatologia , Ligamentos Articulares/fisiopatologia , Manguito Rotador/fisiopatologia , Articulação do Ombro/fisiopatologia , Idoso , Fenômenos Biomecânicos , Feminino , Humanos , Ligamentos Articulares/lesões , Masculino , Pessoa de Meia-Idade , Amplitude de Movimento Articular/fisiologia , Lesões do Manguito Rotador , Lesões do Ombro
18.
Tissue Eng ; 6(5): 531-7, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11074939

RESUMO

In biomedical research, agarose gel is widely used in tissue culture systems because it permits growing cells and tissues in a three-dimensional suspension. This is especially important in the application of tissue engineering concepts to cartilage repair because it supports the cartilage phenotype. Mechanical loading, especially compression, plays a fundamental role in the development and repair of cartilage. It would be advantageous to develop a system where cells and tissues could be subjected to compression so that their responses can be studied. There is currently no information on the pressure response of agarose gel when pressure is applied to the gas phase of a culture system. To understand the transmission of pressure through the gel, we set up an apparatus that would mimic an agarose suspension tissue culture system. This consisted of a sealed metal cylinder containing air as well as a layer of agarose submerged in culture medium. Pressure responses were recorded in the air, fluid, gel center, and gel periphery using various frequencies, pressures, gel volumes, and viscosities. Regression analyses show an almost perfect linear relation between gas and gel pressures (r(2) = 0.99987, p < 0.0001, f(x) = 0.9982 x - 0.0286). The pressure transmission was complete and immediate, throughout the range of the applied pressures, frequencies, volumes, and viscosities tested. Applying dynamic pressure to the gas phase results in reproducible pressure in the agarose and, therefore, validates the use of agarose tissue culture systems in studies employing dynamic pressurization in cartilage tissue engineering.


Assuntos
Géis/química , Sefarose/química , Pressão
19.
J Appl Physiol (1985) ; 92(4): 1494-8, 2002 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11896015

RESUMO

As there is evidence that ligamentous laxity is affected by the female hormones, we hypothesized that hormonal changes occurring during pregnancy could have a therapeutic role in preventing the development of a joint contracture. Knee joint contractures were created in pregnant and nonpregnant rats. After 2 wk of immobilization, the degree of contracture was measured with structural properties of the medial collateral and anterior cruciate ligaments and the pubic symphysis. Although not statistically significant, there was a general trend toward reduced contracture in pregnant compared with nonpregnant rats. Cutting the posterior capsule significantly decreased contracture for both the pregnant and nonpregnant groups, confirming the contribution of capsular structures to contracture. Ultimate loads of the medial collateral and anterior cruciate ligaments significantly decreased after immobilization compared with control, but there was no significant effect due to pregnancy. Stiffness and ultimate load of the pubic symphysis were not significantly different between pregnant and nonpregnant groups. The trend toward reduced contracture with pregnancy points toward a possible therapeutic role for female hormones in the prevention of postoperative and/or posttraumatic joint contracture.


Assuntos
Contratura/fisiopatologia , Imobilização/efeitos adversos , Articulação do Joelho/fisiopatologia , Prenhez/fisiologia , Animais , Ligamento Cruzado Anterior/fisiopatologia , Fenômenos Biomecânicos , Contratura/etiologia , Contratura/prevenção & controle , Estrogênios/metabolismo , Feminino , Imobilização/fisiologia , Ligamento Colateral Médio do Joelho/fisiopatologia , Gravidez , Ratos , Ratos Sprague-Dawley , Relaxina/metabolismo
20.
J Orthop Res ; 18(5): 829-34, 2000 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-11117307

RESUMO

The subscapularis muscle is an important mover and stabilizer of the glenohumeral joint. The purpose of this study was to measure regional variations in the structural properties of the subscapularis tendon in two joint positions. Subscapularis tendons from cadaveric shoulders were divided into four sections superiorly to inferiorly and tested to failure at 0 or 60 degrees of glenohumeral abduction. Arm position had a significant influence on stiffness in the inferior and superior portions (p < 0.05). The inferior region showed a higher stiffness in the hanging-arm position (0 degrees) than at 60 degrees of abduction (27.4+/-17.7 compared with 9.5+/-5.9 N/mm). Meanwhile, stiffness of the superior portion was higher at 60 degrees of abduction than in the hanging-arm position (208.7+/-60.9 compared with 147.2+/-32.3 N/mm). In the hanging-arm position (0 degrees) and at 60 degrees of abduction, the superior and midsuperior portions failed at significantly higher loads (superior: 623.2+/-198.6 and 478.2+/-206.6 N at 0 and 60 degrees of abduction, respectively; midsuperior: 706.2+/-164.6 and 598.4+/-268.4 N, respectively) than did the inferior portion (75.1+/-54.2 and 30.3+/-13.0 N, respectively). Likewise, stiffness of the superior and midsuperior portions was significantly higher than that of the inferior region in both positions. Higher stiffness and ultimate load in the superior tendon region may explain the infrequent extension of rotator cuff tears into the subscapularis tendon. Conversely, the significantly lower ultimate load and stiffness in the inferior tendon region could facilitate anterior dislocation of the humeral head when this portion stabilizes the joint in a dislocated position. Therefore, repair of torn inferior portions of the subscapularis tendon should be considered in surgery for glenohumeral instability.


Assuntos
Articulação do Ombro , Tendões/fisiologia , Idoso , Idoso de 80 Anos ou mais , Cadáver , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Maleabilidade , Estresse Mecânico , Suporte de Carga
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