Environmental and socioeconomic impacts of utilizing waste for biochar in rural areas in Indonesia--a systems perspective.

Biochar is the product of incomplete combustion (pyrolysis) of organic material. In rural areas, it can be used as a soil amendment to increase soil fertility. Fuel-constrained villagers may however prefer to use biochar briquettes as a higher-value fuel for cooking over applying it to soils. A systems-oriented analysis using life cycle assessment (LCA) and cost benefit analysis (CBA) was conducted to analyze these two alternative uses of biochar, applying the study to a rural village system in Indonesia. The results showed soil amendment for enhanced agricultural production to be the preferential choice with a positive benefit to the baseline scenario of -26 ecopoints (LCA) and -173 USD (CBA) annually pr. household. In this case, the positive effects of carbon sequestration to the soil and the economic value of the increased agricultural production outweighed the negative environmental impacts from biochar production and the related production costs. Use of biochar in briquettes for cooking fuel yielded negative net effects in both the LCA and CBA (85 ecopoints and 176 USD), even when positive health effects from reduced indoor air pollution were included. The main reasons for this are that emissions during biochar production are not compensated by carbon sequestration and that briquette making is labor-intensive. The results emphasize the importance of investigating and documenting the carbon storage effect and the agricultural benefit in biochar production-utilization systems for a sustainable use. Further research focus on efficient production is necessary due to the large environmental impact of biochar production. In addition, biochar should continue to be used in those soils where the agricultural effect is most beneficial.

Phylogenetic and functional diversity of alkane degrading bacteria associated with Italian ryegrass (Lolium multiflorum) and Birdsfoot trefoil (Lotus corniculatus) in a petroleum oil-contaminated environment.

Twenty-six different plant species were analyzed regarding their performance in soil contaminated with petroleum oil. Two well-performing species, Italian ryegrass (Lolium multiflorum var. Taurus), Birdsfoot trefoil (Lotus corniculatus var. Leo) and the combination of these two plants were selected to study the ecology of plant-associated, culturable alkane-degrading bacteria. Hydrocarbon degrading bacteria were isolated from the rhizosphere, root interior and shoot interior and subjected to the analysis of 16S rRNA gene, the 16S and 23S rRNA intergenic spacer region and alkane hydroxylase genes. Furthermore, we investigated whether alkane hydroxylase genes are plasmid located. Higher numbers of culturable, alkane-degrading bacteria were associated with Italian ryegrass, which were also characterized by a higher diversity, particularly in the plant interior. Only half of the isolated bacteria hosted known alkane hydroxylase genes (alkB and cytochrome P153-like). Degradation genes were found both on plasmids as well as in the chromosome. In regard to application of plants for rhizodegradation, where support of numerous degrading bacteria is essential for efficient break-down of pollutants, Italian ryegrass seems to be more appropriate than Birdsfoot trefoil.

Expression of alkane monooxygenase (alkB) genes by plant-associated bacteria in the rhizosphere and endosphere of Italian ryegrass (Lolium multiflorum L.) grown in diesel contaminated soil.

For phytoremediation of organic contaminants, plants have to host an efficiently degrading microflora. To assess the role of endophytes in alkane degradation, Italian ryegrass was grown in sterile soil with 0, 1 or 2% diesel and inoculated either with an alkane degrading bacterial strain originally derived from the rhizosphere of Italian ryegrass or with an endophyte. We studied plant colonization of these strains as well as the abundance and expression of alkane monooxygenase (alkB) genes in the rhizosphere, shoot and root interior. Results showed that the endophyte strain better colonized the plant, particularly the plant interior, and also showed higher expression of alkB genes suggesting a more efficient degradation of the pollutant. Furthermore, plants inoculated with the endophyte were better able to grow in the presence of diesel. The rhizosphere strain colonized primarily the rhizosphere and showed low alkB gene expression in the plant interior.