The effect of bone defect size on the 3D accuracy of alveolar bone augmentation performed with additively manufactured patient-specific titanium mesh.

OBJECTIVE: Additively manufactured (3D-printed) titanium meshes have been adopted in the dental field as non-resorbable membranes for guided bone regeneration (GBR) surgery. However, according to previous studies, inaccuracies between planned and created bone volume and contour are common, and many reasons have been speculated to affect its accuracy. The size of the alveolar bone defect can significantly increase patient-specific titanium mesh design and surgical difficulty. Therefore, this study aimed to analyze and investigate the effect of bone defect size on the 3D accuracy of alveolar bone augmentation performed with additively manufactured patient-specific titanium meshes. METHODS: Twenty 3D-printed patient-specific titanium mesh GBR surgery cases were enrolled, in which 10 cases were minor bone defect/augmentation (the planned bone augmentation surface area is less than or equal to 150 mm2 or one tooth missing or two adjacent front-teeth/premolars missing) and another 10 cases were significant bone defect/augmentation (the planned bone augmentation surface area is greater than 150 mm2 or missing adjacent teeth are more than two (i.e. ≥ three teeth) or missing adjacent molars are ≥ two teeth). 3D digital reconstruction/superposition technology was employed to investigate the bone augmentation accuracy of 3D-printed patient-specific titanium meshes. RESULTS: There was no significant difference in the 3D deviation distance of bone augmentation between the minor bone defect/augmentation group and the major one. The contour lines of planned-CAD models in two groups were basically consistent with the contour lines after GBR surgery, and both covered the preoperative contour lines. Moreover, the exposure rate of titanium mesh in the minor bone defect/augmentation group was slightly lower than the major one. CONCLUSION: It can be concluded that the size of the bone defect has no significant effect on the 3D accuracy of alveolar bone augmentation performed with the additively manufactured patient-specific titanium mesh.

Strain Monitoring of a Composite Drag Strut in Aircraft Landing Gear by Fiber Bragg Grating Sensors.

This work reports on the use of Fiber Bragg Grating (FBG) sensors integrated with innovative composite items of aircraft landing gear for strain/stress monitoring. Recently, the introduction of innovative structures in aeronautical applications is appealing with two main goals: (i) to decrease the weight and cost of current items; and (ii) to increase the mechanical resistance, if possible. However, the introduction of novel structures in the aeronautical field demands experimentation and certification regarding their mechanical resistance. In this work, we successfully investigate the possibility to use Fiber Bragg Grating sensors for the structural health monitoring of innovative composite items for the landing gear. Several FBG strain sensors have been integrated in different locations of the composite item including region with high bending radius. To optimize the localization of the FBG sensors, load condition was studied by Finite Element Method (FEM) numerical analysis. Several experimental tests have been done in range 0-70 kN by means of a hydraulic press. Obtained results are in very good agreement with the numerical ones and demonstrate the great potentialities of FBG sensor technology to be employed for remote and real-time load measurements on aircraft landing gears and to act as early warning systems.

Thermokinetic and Chemorheology of the Geopolymerization of an Alumina-Rich Alkaline-Activated Metakaolin in Isothermal and Dynamic Thermal Scans.

Alkaline sodium hydroxide/sodium silicate-activating high-purity metakaolin geopolymerization is described in terms of metakaolin deconstruction in tetrahedral hydrate silicate [O[Si(OH)3]]- and aluminate [Al(OH)4]- ionic precursors followed by their reassembling in linear and branched sialates monomers that randomly copolymerize into an irregular crosslinked aluminosilicate network. The novelty of the approach resides in the concurrent thermo-calorimetric (differential scanning calorimetry, DSC) and rheological (dynamic mechanical analysis, DMA) characterizations of the liquid slurry during the transformation into a gel and a structural glassy solid. Tests were run either in temperature scan (1 °C/min) or isothermal (20 °C, 30 °C, 40 °C) cure conditions. A Gaussian functions deconvolution method has been applied to the DSC multi-peak thermograms to separate the kinetic contributions of the oligomer's concurrent reactions. DSC thermograms of all tested materials are well-fitted by a combination of three overlapping Gaussian curves that are associated with the initial linear low-molecular-weight (Mw) oligomers (P1) formation, oligomers branching into alumina-rich and silica-rich gels (P2), and inter- and intra-molecular crosslinking (P3). The loss factor has been used to define viscoelastic behavioral zones for each DMA rheo-thermogram operated in the same DSC thermal conditions. Macromolecular evolution and viscoelastic properties have been obtained by pairing the deconvoluted DSC thermograms with the viscoelastic behavioral zones of the DMA rheo-thermograms. Two main chemorheological behaviors have been identified relative to pre- and post-gelation separation of the viscoelastic liquid from the viscoelastic solid. Each comprises three behavioral zones, accounting for the concurrently occurring linear and branching oligomerization, aluminate-rich and silica-rich gel nucleations, crosslinking, and vitrification. A "rubbery plateau" in the loss factor path, observed for all the testing conditions, identifies a large behavioral transition zone dividing the incipient gelling liquid slurry from the material hard setting and vitrification.

Geopolymer Materials for Bone Tissue Applications: Recent Advances and Future Perspectives.

With progress in the bone tissue engineering (BTE) field, there is an important need to develop innovative biomaterials to improve the bone healing process using reproducible, affordable, and low-environmental-impact alternative synthetic strategies. This review thoroughly examines geopolymers' state-of-the-art and current applications and their future perspectives for bone tissue applications. This paper aims to analyse the potential of geopolymer materials in biomedical applications by reviewing the recent literature. Moreover, the characteristics of materials traditionally used as bioscaffolds are also compared, critically analysing the strengths and weaknesses of their use. The concerns that prevented the widespread use of alkali-activated materials as biomaterials (such as their toxicity and limited osteoconductivity) and the potentialities of geopolymers as ceramic biomaterials have also been considered. In particular, the possibility of targeting their mechanical properties and morphologies through their chemical compositions to meet specific and relevant requirements, such as biocompatibility and controlled porosity, is described. A statistical analysis of the published scientific literature is presented. Data on "geopolymers for biomedical applications" were extracted from the Scopus database. This paper focuses on possible strategies necessary to overcome the barriers that have limited their application in biomedicine. Specifically, innovative hybrid geopolymer-based formulations (alkali-activated mixtures for additive manufacturing) and their composites that optimise the porous morphology of bioscaffolds while minimising their toxicity for BTE are discussed.

Bio-Resorption Control of Magnesium Alloy AZ31 Coated with High and Low Molecular Weight Polyethylene Oxide (PEO) Hydrogels.

Magnesium AZ31 alloy has been chosen as bio-resorbable temporary prosthetic implants to investigate the degradation processes in a simulating body fluid (SBF) of the bare metal and the ones coated with low and high-molecular-weight PEO hydrogels. Hydrogel coatings are proposed to control the bioresorption rate of AZ31 alloy. The alloy was preliminary hydrothermally treated to form a magnesium hydroxide layer. 2 mm discs were used in bioresorption tests. Scanning electron microscopy was used to characterize the surface morphology of the hydrothermally treated and PEO-coated magnesium alloy surfaces. The variation of pH and the mass of Mg2+ ions present in the SBF corroding medium have been monitored for 15 days. Corrosion current densities (Icorr) and corrosion potentials (Ecorr) were evaluated from potentiodynamic polarisation tests on the samples exposed to the SBF solution. Kinetics of cumulative Mg ions mass released in the corroding solution have been evaluated regarding cations diffusion and mass transport parameters. The initial corrosion rates for the H- and L-Mw PEO-coated specimens were similar (0.95 ± 0.12 and 1.82 ± 0.52 mg/cm2day, respectively) and almost 4 to 5 times slower than that of the uncoated system (6.08 mg/cm2day). Results showed that the highly swollen PEO hydrogel coatings may extend into the bulk solution, protecting the coated metal and efficiently controlling the degradation rate of magnesium alloys. These findings focus more research effort on investigating such systems as tunable bioresorbable prosthetic materials providing idoneous environments to support cells and bone tissue repair.

Chemorheology of a Si/Al > 3 Alkali Activated Metakaolin Paste through Parallel Differential Scanning Calorimetry (DSC) and Dynamic Mechanical Analysis (DMA).

Although geopolymers, as structural materials, should have superior engineering properties than traditional cementitious materials, they often need to improve their final characteristics' reproducibility due to the need for more control of the complex silico-aluminate decomposition and polymerisation stages. Thermosetting of a reactive geopolymeric paste involves tetrahedral Silicate and Aluminate precursor condensation into polyfunctional oligomers of progressively higher molecular weight, transforming the initial liquid into a gel and a structural solid. Viscosity and gelation control become particularly critical when the geopolymer is processed with 3D printing additive technology. Its physical state modification kinetics should match the flow and setting characteristics required by the deposition process. The reaction kinetics and the elastic and viscous characteristics preceding gelation and hardening have been investigated for an alkali-activated Metakaolin/Sodium Silicate-Sodium Hydroxide paste with a Si/Al ratio > 3. A chemoreological approach has been extended to these inorganic polymerisable systems, as already utilised for organic thermosetting polymers. Differential scanning calorimetry and Oscillatory DMA were carried out to monitor the advancement of the polymerisation reaction and the associated variations of the rheological viscoelastic properties. Dynamic thermal scans were run at 1 °C/min and a frequency of 10 Hz for the dynamic mechanical tests. The observed kinetics of polymerisation and variations of the elastic and viscous components of the complex viscosities and shear moduli are described in terms of polycondensation of linear and branched chains of oligomeric macromolecules of increasing complexity and molecular weight up to gelation (Gel1) and cross-linking of the gelled macrostructure (Gel2) and final glassy state. Geopolymerization can be allocated into two main behavioural zones: a viscoelastic liquid paste below 32.5% of reaction advancement and a viscoelastic solid above. Initial complex viscosities range from 2.3 ± 0.9 × 10-5 MPa*s to 6.8 ± 0.9 × 10-2 in the liquid-like state and from 1.9 ± 0.1 MPa to 9.6 ± 2.1 × 102 MPa in the solid-like state.

Geopolymer Materials for Extrusion-Based 3D-Printing: A Review.

This paper examines how extrusion-based 3D-printing technology is evolving, utilising geopolymers (GPs) as sustainable inorganic aluminosilicate materials. Particularly, the current state of 3D-printing geopolymers is critically examined in this study from the perspectives of the production process, printability need, mix design, early-age material features, and sustainability, with an emphasis on the effects of various elements including the examination of the fresh and hardened properties of 3D-printed geopolymers, depending on the matrix composition, reinforcement type, curing process, and printing configuration. The differences and potential of two-part and one-part geopolymers are also analysed. The applications of advanced printable geopolymer materials and products are highlighted, along with some specific examples. The primary issues, outlooks, and paths for future efforts necessary to advance this technology are identified.

Effectiveness of biomechanically stable pergola-like additively manufactured scaffold for extraskeletal vertical bone augmentation.

Objective: Extraskeletal vertical bone augmentation in oral implant surgery requires extraosseous regeneration beyond the anatomical contour of the alveolar bone. It is necessary to find a better technical/clinical solution to solve the dilemma of vertical bone augmentation. 3D-printed scaffolds are all oriented to general bone defect repair, but special bone augmentation design still needs improvement. Methods: This study aimed to develop a structural pergola-like scaffold to be loaded with stem cells from the apical papilla (SCAPs), bone morphogenetic protein 9 (BMP9) and vascular endothelial growth factor (VEGF) to verify its bone augmentation ability even under insufficient blood flow supply. Scaffold biomechanical and fluid flow optimization design by finite element analysis (FEA) and computational fluid dynamics (CFD) was performed on pergola-like additive-manufactured scaffolds with various porosity and pore size distributions. The scaffold geometrical configuration showing better biomechanical and fluid dynamics properties was chosen to co-culture for 2 months in subcutaneously into nude mice, with different SCAPs, BMP9, and (or) VEGF combinations. Finally, the samples were removed for Micro-CT and histological analysis. Results: Micro-CT and histological analysis of the explanted scaffolds showed new bone formation in the "Scaffold + SCAPs + BMP9" and the "Scaffold + SCAPs + BMP9 + VEGF" groups where the VEGF addition did not significantly improve osteogenesis. No new bone formation was observed either for the "Blank Scaffold" and the "Scaffold + SCAPs + GFP" group. The results of this study indicate that BMP9 can effectively promote the osteogenic differentiation of SCAPs. Conclusion: The pergola-like scaffold can be used as an effective carrier and support device for new bone regeneration and mineralization in bone tissue engineering, and can play a crucial role in obtaining considerable vertical bone augmentation even under poor blood supply.

Evaluation of surgical placement accuracy of customized CAD/CAM titanium mesh using screws-position-guided template: A retrospective comparative study.

BACKGROUND: Customized computer-aided-design/computer-aided-manufacturing (CAD/CAM) titanium meshes have been adopted for alveolar bone augmentation. But the inaccuracies between planned and created bone volume/contour are quite common, and the surgical placement of the customized mesh was considered as the first critical factor. However, the evaluation of surgical placement accuracy of customized mesh is currently lacking. PURPOSE: The aim of this study was to evaluate the accuracy of the surgical placement of customized meshes. METHODS: A total of 30 cases, 20 without the screws-position-guided template and 10 with the screws-position-guided template, were included in this study. The cone beam CT (CBCT) data sets of pre- and postoperative were converted into 3D models and digitally aligned. Then the actual placement of customized mesh and retainer titanium screws was compared to the virtual one to assess the surgical placement accuracy of customized mesh. At least 6 months after surgery, a new CBCT was taken and converted into 3D models. Planned bone volume, created bone volume, vertical bone augmentation, healing complications rate, pseudo-periosteum rate, exposure rate, and infection rate were all evaluated. RESULTS: The 3D digital reconstruction/registration analysis showed that the average difference between actual placement and planned one of customized mesh in positive and negative directions was 2.69 ± 0.70 mm and -1.41 ± 0.90 mm, respectively, without the screws-position-guided template. And the mean difference values between the actual and planned placement of the screws on the X and Y axes were 0.74 ± 0.85 mm and 0.89 ± 0.84 mm. In contrast, with the screws-position-guided template, the results were 2.38 ± 0.69 mm and -1.30 ± 1.13 mm. Accordingly, the mean difference values of screws were 0.76 ± 0.84 mm and 0.94 ± 0.72 mm. There was no statistical difference between the two groups, and the noninferiority of the control group compared to the test group was also confirmed by the comparative analysis. CONCLUSION: It can be concluded that there is a certain deviation between the planned surgical placement and actual one of customized mesh, and using screws-position-guided template is of limited help for its accurate placement. Further research is needed to achieve precise surgical placement of the customized mesh to achieve precise alveolar bone augmentation.

An innovative additively manufactured implant for mandibular injuries: Design and preparation processes based on simulation model.

Objective: For mandibular injury, how to utilize 3D implants with novel structures to promote the reconstruction of large mandibular bone defect is the major focus of clinical and basic research. This study proposed a novel 3D titanium lattice-like implant for mandibular injuries based on simulation model, which is designed and optimized by a biomechanical/mechanobiological approach, and the working framework for optimal design and preparation processes of the implant has been validated to tailored to specific patient biomechanical, physiological and clinical requirements. Methods: This objective has been achieved by matching and assembling different morphologies of a lattice-like implant mimicking cancellous and cortical bone morphologies and properties, namely, an internal spongy trabecular-like structure that can be filled with bone graft materials and an external grid-like structure that can ensure the mechanical bearing capacity. Finite element analysis has been applied to evaluate the stress/strain distribution of the implant and bone graft materials under physiological loading conditions to determine whether and where the implant needs to be optimized. A topological optimization approach was employed to improve biomechanical and mechanobiological properties by adjusting the overall/local structural design of the implant. Results: The computational results demonstrated that, on average, values of the maximum von-Mises stress in the implant model nodes could be decreased by 43.14% and that the percentage of optimal physiological strains in the bone graft materials can be increased from 35.79 to 93.36% since early regeneration stages. Metal additive manufacturing technology was adopted to prepare the 3D lattice-like implant to verify its feasibility for fabrication. Following the working framework proposed in this study, the well-designed customized implants have both excellent biomechanical and mechanobiological properties, avoiding mechanical failure and providing sufficient biomechanical stimuli to promote new bone regeneration. Conclusion: This study is expected to provide a scientific and feasible clinical strategy for repairing large injuries of mandibular bone defects by offering new insights into design criteria for regenerative implants.

Influence of abutment material on the fracture strength and failure modes of abutment-fixture assemblies when loaded in a bio-faithful simulation.

OBJECTIVES: The aim of the present study was to evaluate differences in the ultimate fracture resistance of titanium and zirconia abutments. MATERIAL AND METHODS: Twenty titanium fixtures were embedded in 20 resin mandible section simulators to mimic osseointegrated implants in the premolar area. The embedded implants were then randomly divided into two groups. Afterwards, specimens in group A (n=10) were connected to titanium abutments (TiDesign™ 3.5/4.0, 5.5, 1.5 mm), while specimens in group B (n=10) were connected to zirconia abutments (ZirDesign ™ 3.5/4.0, 5.5, 1.5 mm). Both groups were loaded to failure in a dynamometric testing machine. Fractured samples were then analyzed by scanning electron microscopy (SEM). RESULTS: Group A showed a significantly higher fracture strength than that observed in group B. Group A failures were observed at the screw that connects the abutment with the implant while the abutment connection hexagons were plastically bent by the applied load. Group B failures were a result of abutment fractures. SEM analysis showed that in group A the screw failure was driven by crack nucleation, coalescence and propagation, while in group B, the SEM analysis of failed surfaces showed the conchoidal fracture profile characteristic of brittle materials. CONCLUSIONS: The strength of both tested systems is adequate to resist physiologic chewing forces in the premolar area. Conversely, the titanium and zirconia failure modes evaluated here occurred at unphysiological loads. In addition, because the abutments were tested without crowns, the presented data have limited direct transfer to the clinical situation.

Biomechanically Tunable Nano-Silica/P-HEMA Structural Hydrogels for Bone Scaffolding.

Innovative tissue engineering biomimetic hydrogels based on hydrophilic polymers have been investigated for their physical and mechanical properties. 5% to 25% by volume loading PHEMA-nanosilica glassy hybrid samples were equilibrated at 37 °C in aqueous physiological isotonic and hypotonic saline solutions (0.15 and 0.05 M NaCl) simulating two limiting possible compositions of physiological extracellular fluids. The glassy and hydrated hybrid materials were characterized by both dynamo-mechanical properties and equilibrium absorptions in the two physiological-like aqueous solutions. The mechanical and morphological modifications occurring in the samples have been described. The 5% volume nanosilica loading hybrid nanocomposite composition showed mechanical characteristics in the dry and hydrated states that were comparable to those of cortical bone and articular cartilage, respectively, and then chosen for further sorption kinetics characterization. Sorption and swelling kinetics were monitored up to equilibrium. Changes in water activities and osmotic pressures in the water-hybrid systems equilibrated at the two limiting solute molarities of the physiological solutions have been related to the observed anomalous sorption modes using the Flory-Huggins interaction parameter approach. The bulk modulus of the dry and glassy PHEMA-5% nanosilica hybrid at 37 °C has been observed to be comparable with the values of the osmotic pressures generated from the sorption of isotonic and hypotonic solutions. The anomalous sorption modes and swelling rates are coherent with the difference between osmotic swelling pressures and hybrid glassy nano-composite bulk modulus: the lower the differences the higher the swelling rate and equilibrium solution uptakes. Bone tissue engineering benefits of the use of tuneable biomimetic scaffold biomaterials that can be "designed" to act as biocompatible and biomechanically active hybrid interfaces are discussed.

Nonlinear visco-elastic finite element analysis of porcelain veneers: a submodelling approach to strain and stress distributions in adhesive and resin cement.

PURPOSE: To assess under load the biomechanical behavior of the cementing system of feldspathic vs alumina porcelain veneers. MATERIALS AND METHODS: A 3D model of a maxillary central incisor, the periodontal ligament (PDL) and the alveolar bone was generated. Incisors restored with alumina and feldspathic porcelain veneers were compared to a natural sound tooth. Enamel, cementum, cancellous and cortical bone were considered isotropic elastic materials; conversely, dentin was designated as orthotropic. The nonlinear visco-elatic behavior of the PDL was considered. The adhesive layers were modelled using spring elements. A 50-N load at a 60-degree angle to the tooth's longitudinal axis was applied and validated. Stress concentration in the interfacial volumes of the main models was identified and submodelled in a new environment. RESULTS: Regarding tooth structure, strain concentrations were observed in the root dentin below the CEJ. As to the cement layer, tensile stresses concentrated in the palatal margin of the adhesive complex. CONCLUSION: Despite the effects on tooth deformation, the rigidity of the veneer did not affect the stress distributions in the cement layer or in the adhesive layers. In both cases, the palatal and cervical margins seemed to be the most stressed areas.

Effect of Porous Microstructures on the Biomechanical Characteristics of a Root Analogue Implant: An Animal Study and a Finite Element Analysis.

BACKGROUND: Full ceramic or metal custom-made root analogue implants (RAIs) are made by replicating the natural tooth geometry. However, it may lead to the stress shielding of the surrounding bone, and an RAI is unable to easily achieve primary stability. Therefore, to improve primary stability and reduce stress shielding, RAI porous structures are proposed. The purpose of this study was to evaluate the effect of porous microstructures on the biomechanical characteristics of the custom-made RAI. METHODS: Porous and bulk titanium cylinders and porous RAI and conventional implants for in vivo tests were fabricated using a selective laser melting (SLM) technology. The elastic modulus and the compressive strength of porous titanium cylinders were evaluated. These samples were then implanted into rabbit femurs (cylinders) and beagle dog mandibles (RAI and conventional implants). A simplified three-dimensional geometry of the anterior maxilla of a patient was constructed. Then, based on the extracted standard template library (STL) data, five different RAI models were constructed: (A) smooth surface, (B) pit surface, (C) bulb surface, (D) threaded surface, and (E) porous surface. A conventional implant model was also constructed. A static load of 100 N was applied to the crown in the multivectoral direction. RESULTS: The results of the in vivo experiment confirmed that the porous structure decreased the elastic modulus of Ti6Al4V. Additionally, the implantation of the porous custom-made RAIs resulted in increased new bone ingrowth and decreased bone resorption compared to conventional implants. Moreover, the 3D finite element analysis suggested that the bone surrounding porous custom-made RAIs was subjected to a more uniform stress distribution, and the strain values of the surrounding bone were more conducive to bone formation. CONCLUSION: Based on these findings, a custom-made RAI with a porous surface accelerates bone formation and might reduce the stress-shielding effect.

Implant-to-bone force transmission: a pilot study for in vivo strain gauge measurement technique.

The experimental determination of local bone deformations due to implant loading would allow for a better understanding of the biomechanical behavior of the bone-implant-prosthesis system as well as the influence of uneven force distribution on the onset of implant complications. The present study aimed at describing an innovative in vivo strain gauge measurement technique to evaluate implant-to-bone force transmission, assessing whether and how oral implants can transfer occlusal forces through maxillary bones. In vivo force measurements were performed in the maxillary premolar region of a male patient who had previously received a successful osseointegrated titanium implant. Three linear mini-strain gauges were bonded onto three different buccal cortical bone locations (i.e. coronal, middle, apical) and connected to strain measuring hardware and software. A customized screw-retained abutment was manufactured to allow for vertical and horizontal loading tests. As to the vertical load test, the patient was instructed to bite on a load cell applying his maximum occlusal force for 20 s and then recovering for 10 s to restore the bone unstrained state; the test was repeated 20 times consecutively. As regards the horizontal load test, the implant was subjected to a total of 20 load applications with force intensities of 5 and 10 kg. During the tests, the recorded signals were plotted in real time on a graph as a function of time by means of a strain analysis software. The described strain gauge measurement technique proved to be effective in recording the forces transmitted from osseointegrated implants to the cortical bone. Horizontal loads caused higher deformations of cortical bone than vertical biting forces; in both situations, the deformation induced by the force transferred from the implant to the bone progressively decreased from the coronal to the apical third of the alveolar ridge. At approximately 9 mm from the implant neck, the effect of occlusal force transmission through osseointegrated titanium implants was negligible if compared to the apical region.

Effect of simulated microgravity induced PI3K-nos2b signalling on zebrafish cardiovascular plexus network formation.

Local abnormal angiogenesis and cardiovascular system reorganization have been observed in embryos exposed to a simulated microgravity (SM) environment. In this study, changes in key molecular signals and pathways in cardiovascular development have been investigated under microgravity conditions. In particular, the caudal vein plexus (CVP) network, formed by sprouting angiogenesis has been chosen. Zebrafish embryos were exposed to SM using a ground-based microgravity bioreactor for 24 and 36 h. The SM was observed to have no effect on the zebrafish length, tail width and incubation time whereas it was observed to significantly reduce the heart rate frequency and to promote abnormal development of the CVP network in the embryos. Nitric oxide (NO) content demonstrated that the total proteins in zebrafish embryos were significantly higher in SM than in the control group grown under normal conditions. It was then preliminarily determined how NO signals were involved in SM regulated zebrafish CVP network formation. nos2b MO was injected and CVP network evolution was observed in 36 h post fertilization (hpf) under SM condition. The results showed that the CVP network formation was considerably decreased in the nos2b MO treated group. However, this inhibition of the CVP network development was not observed in control MO group, indicating that nos2b is involved in the SM-regulated vascular development process in zebrafish. Moreover, specific phosphoinositide 3-kinase (PI3K) inhibitors such as LY294002 were also tested on zebrafish embryos under SM condition. This treatment significantly inhibited the formation of zebrafish CVP network. Furthermore, overexpression of nos2b partly rescued the LY294002-caused CVP network failure. Therefore, it can be concluded that SM affects zebrafish CVP network remodeling by enhancing angiogenesis. Additionally, the PI3K-nos2b signaling pathway is involved in this process.

Non-linear viscoelastic finite element analysis of the effect of the length of glass fiber posts on the biomechanical behaviour of directly restored incisors and surrounding alveolar bone.

The study aimed at estimating the effect of insertion length of posts with composite restorations on stress and strain distributions in central incisors and surrounding bone. The typical, average geometries were generated in a FEA environment. Dentin was considered as an elastic orthotropic material, and periodontal ligament was coupled with nonlinear viscoelastic mechanical properties. The model was then validated with experimental data on displacement of incisors from published literature. Three post lengths were investigated in this study: root insertion of 5, 7, and 9 mm. For control, a sound incisor model was generated. Then, a tearing load of 50 N was applied to both sound tooth and simulation models. Post restorations did not seem to affect the strain distribution in bone when compared to the control. All simulated post restorations affected incisor biomechanics and reduced the root's deforming capability, while the composite crowns underwent a higher degree of deformation than the sound crown. No differences could be noticed in incisor stress and strain. As for the influence of post length, it was not shown to affect the biomechanics of restored teeth.

[A case of severe metformin-associated lactic acidosis treated with CVVHDF and regional anticoagulation with sodium citrate].

Metformin is an antidiabetic drug; used to treat type II diabetes mellitus, metformin associated lactic acidosis has an incidence of 2-9 cases / 100,000 patients / year with high mortality (30%). We have had the case of a 75-year-old woman with metabolic acidosis as a result of metformin assumption, treated by renal replacement therapy (CRRT) with continuous veno-venous hemodiafiltration (CVVHDF). RESULTS: after a short treatment period there was a reduction in Lactates (from 16.8 mmol/L to 12.6 mmol/L) and a progressive improvement of acidosis. In 72 hours the recovery of diuresis and subsequent suspension of CRRT was achieved. CONCLUSION: CRRT, in addition to ensuring support for renal failure and volume correction, allowed a rapid recovery from metformin-associated lactic acidosis.

Three-dimensional finite element analysis of strain and stress distributions in endodontically treated maxillary central incisors restored with different post, core and crown materials.

OBJECTIVES: The present comparative analysis aimed at evaluating which combination of restorative materials resulted in the most homogeneous stress and strain distributions. METHODS: A three-dimensional finite element analysis was performed. All the nodes on the external surface of the root were constrained in all directions. Eighteen experimental models with different material properties and configurations were simulated. An arbitrary load of 10N was applied at 60 degrees angle with tooth longitudinal axis on the palatal surface of the crown. Von Mises (equivalent stresses) energetic criterion was chosen. RESULTS: In all the models the values of both strain and stress recorded at the middle third of the buccal aspect of the root surface were at their maxima. On the contrary, the minimum values were noticed at level of both the apical portion of the post and the root apex. The maximum stresses were evidenced at level of the cemento-enamel junction (CEJ) on both the buccal and palatal aspects of root cement and dentin. Stress progressively decreased from the outer to the inner part of the root and from the CEJ towards the incisal margin of the crown as well. SIGNIFICANCE: The results of the present study would allow clinicians to make an informed choice from among available materials to restore endodontically treated teeth.

Three-dimensional finite element analysis of stress and strain distributions in post-and-core treated maxillary central incisors.

PURPOSE: To estimate which combination of restorative materials resulted in the most homogeneous stress and strain distributions in post-and-core treated teeth. MATERIALS AND METHODS: Eight experimental finite element models with different material configurations were simulated; both indirect and direct restorations were considered. An arbitrary load of 50 N was applied on the palatal surface of the crown at a 60-degree angle to the tooth's longitudinal axis to simulate tearing function. RESULTS: In all the models, the values of both strain and stress recorded in the middle third of the buccal aspect of the root surface were off the scale. In contrast, the minimum values were noticed at the level of both the apical portion of the post and the root apex. CONCLUSION: The mechanical properties of the crown and core materials influenced both the position of concentration areas and the level of stress and strain along the dentin/cement/post interfaces.