Assuntos
Genes de Protozoários , Família Multigênica , Proteínas de Protozoários , Trypanosoma cruzi/crescimento & desenvolvimento , Trypanosoma cruzi/genética , Glicoproteínas Variantes de Superfície de Trypanosoma/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Clonagem Molecular , Regulação da Expressão Gênica , Genoma de Protozoário , Estágios do Ciclo de Vida , Glicoproteínas de Membrana/genética , Dados de Sequência Molecular , Alinhamento de Sequência , Trypanosoma cruzi/metabolismo , Glicoproteínas Variantes de Superfície de Trypanosoma/metabolismo , Glicoproteínas Variantes de Superfície de Trypanosoma/farmacologiaRESUMO
We report the molecular characterization of a novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Steady-state level of transcripts of this sequence family appeared to be developmentally regulated, since only in the replicative forms the parasite showed expression of related sequences with a major band around 3 kb. The presence of frame shifts or premature stop codons predicts that transcripts are not translated. The sequence family also contains truncated forms of retrotransposons elements that may become potential hot spots for retroelement insertion. Sequences homologous to this family are interspersed at many chromosomes including the subtelomeric regions.
Assuntos
DNA de Protozoário/genética , Genoma de Protozoário , Sequências Repetitivas Dispersas/genética , Trypanosoma cruzi/genética , AnimaisRESUMO
We report the molecular characterization of a novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Steady-state level of transcripts of this sequence family appeared to be developmentally regulated, since only in the replicative forms the parasite showed expression of related sequences with a major band around 3 kb. The presence of frame shifts or premature stop codons predicts that transcripts are not translated. The sequence family also contains truncated forms of retrotransposons elements that may become potential hot spots for retroelement insertion. Sequences homologous to this family are interspersed at many chromosomes including the subtelomeric regions