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Fouling is a major concern for solid/liquid interfaces of materials used in different applications. One approach of fouling control is the use of hydrophilic polymer coatings made from poly-anions and poly-cations using the layer-by-layer (LBL) method. The authors hypothesized that the poly-anionic properties and the poly-phosphate backbone of DNA would provide anti-biofouling and anti-scaling properties. To this end, poly(ethyleneimine)/DNA LBL coatings against microbial and inorganic fouling were developed, characterized and evaluated. DNA LBL coatings reduced inorganic fouling from tap water by 90% when incubated statically or under flow conditions mimicking surfaces in heat exchangers. The coatings also impaired biofilm formation by 93% on stainless steel from tap water, and resulted in a 97% lower adhesion force and reduced initial attachment of the human pathogens Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa on glass. This study demonstrates a proof of concept that LBL coatings with poly-anions harboring phosphate groups can address fouling in several applications.
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Incrustação Biológica , DNA/análise , Polímeros/química , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus epidermidis/efeitos dos fármacos , Infecções Bacterianas/prevenção & controle , Biofilmes , Carbonato de Cálcio/química , Cátions , Interações Hidrofóbicas e Hidrofílicas , Aço Inoxidável , Propriedades de SuperfícieRESUMO
We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.
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Proteínas Imobilizadas/metabolismo , Molécula 1 de Adesão Intercelular/metabolismo , Monócitos/citologia , Nanoestruturas/química , Podossomos/metabolismo , Adesão Celular , Linhagem Celular , Humanos , Proteínas Imobilizadas/análise , Molécula 1 de Adesão Intercelular/análise , Monócitos/metabolismoRESUMO
Self-assembly provides an effective approach for the fabrication of supramolecular complexes or heterojunction materials, which have unique properties and potential applications in many fields. In this study, the self-assembled structures of stearic acid (SA) and nucleic acid base, guanine (G), are formed at the liquid-solid interface. Two main configurations, namely SA-G-SA and SA-G-G-SA, are observed and the intermolecular recognition mechanism between G and SA is proposed from the hydrogen-bonding point of view.
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Guanina/química , Ácidos Esteáricos/química , Ligação de Hidrogênio , Microscopia de Tunelamento , Conformação Molecular , TermodinâmicaRESUMO
The ability to 'see' down to nanoscale has always been one of the most challenging obstacles for researchers to address fundamental questions. For many years, researchers have been developing scanning probe microscopy techniques to improve imaging capability at nanoscale. Among them, atomic force microscopy (AFM) has received considerable attention, which allows probing topography of biological species at real space under physiological environment. Importantly, force measurements in AFM enable researchers to reveal not only the topography but also the relevant physical-chemical properties. AFM-based dynamic nanomechanical mapping (DNM) provides insights into the functions of biological systems by the interpretation of 'force', which are inaccessible by most of the other analytic techniques. This review is aiming to shed light on these recently developed AFM-based DNM techniques for biomolecular imaging, and discuss the relative applications in biological research from the nanomechanical point of view.
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Microscopia de Força Atômica/métodos , Animais , Fenômenos Biomecânicos , Desenho de Equipamento , Humanos , Microscopia de Força Atômica/instrumentação , Ácidos Nucleicos/análise , Proteínas/análiseRESUMO
Staphylococcus epidermidis causes some of the most hard-to-treat clinical infections by forming biofilms: Multicellular communities of bacteria encased in a protective matrix, supporting immune evasion and tolerance against antibiotics. Biofilms occur most commonly on medical implants, and a key event in implant colonization is the robust adherence to the surface, facilitated by interactions between bacterial surface proteins and host matrix components. S. epidermidis is equipped with a giant adhesive protein, extracellular matrix-binding protein (Embp), which facilitates bacterial interactions with surface-deposited, but not soluble fibronectin. The structural basis behind this selective binding process has remained obscure. Using a suite of single-cell and single-molecule analysis techniques, we show that S. epidermidis is capable of such distinction because Embp binds specifically to fibrillated fibronectin on surfaces, while ignoring globular fibronectin in solution. S. epidermidis adherence is critically dependent on multivalent interactions involving 50 fibronectin-binding repeats of Embp. This unusual, Velcro-like interaction proved critical for colonization of surfaces under high flow, making this newly identified attachment mechanism particularly relevant for colonization of intravascular devices, such as prosthetic heart valves or vascular grafts. Other biofilm-forming pathogens, such as Staphylococcus aureus, express homologs of Embp and likely deploy the same mechanism for surface colonization. Our results may open for a novel direction in efforts to combat devastating, biofilm-associated infections, as the development of implant materials that steer the conformation of adsorbed proteins is a much more manageable task than avoiding protein adsorption altogether.
A usually harmless bacterium called Staphylococcus epidermidis lives on human skin. Sometimes it makes its way into the bloodstream through a cut or surgical procedure, but it rarely causes blood infections. It can, however, cause severe infections when it attaches to the surface of a medical implant like a pacemaker or an artificial replacement joint. It does this by forming a colony of bacteria on the implant's surface called a biofilm, which protects the bacteria from destruction by the immune system or antibiotics. Understanding how Staphylococcus epidermidis implant infections start is critical to preventing them. This information may help scientists develop infection-resistant implants or new treatments for implant infections. Scientists suspect that Staphylococcus epidermidis attaches to implants by binding to a human protein called fibronectin, which coats medical implants in the human body. Another protein on the surface of the bacteria, called Embp, facilitates the connection. But why the bacteria attach to fibronectin on implants, and not fibronectin molecules in the bloodstream, is unclear. Now, Khan, Aslan et al. show that Embp forms a Velcro-like bond with fibronectin on the surface of implants. In the experiments, Khan and Aslan et al. used powerful microscopes to create 3-dimensional images of the interactions between Embp and fibronectin. The experiments showed that Embp's attachment site is hidden on the globe-shaped form of fibronectin circulating in the blood. But when fibronectin covers an implant surface, it forms a fibrous network, and Embp can attach to it with up to 50 Velcro-like individual connections. These many weak connections form a strong bond that withstands the force of blood pumping past. The experiments show that the fibrous coating of fibronectin on implants makes them a hotspot for Staphylococcus epidermidis infections. Finding ways to block Embp from attaching to fibronectin on implants, or altering the form fibronectin takes on implants, may help prevent these infections. Many bacteria that form biofilms have an Embp-like protein. As a result, these discoveries may also help scientists develop prevention or treatment strategies for other bacterial biofilm infections.
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Proteínas de Transporte , Infecções Estafilocócicas , Proteínas de Bactérias/metabolismo , Biofilmes , Proteínas de Transporte/metabolismo , Fibronectinas/metabolismo , Humanos , Infecções Estafilocócicas/microbiologia , Staphylococcus epidermidisRESUMO
Listeria monocytogenes is a foodborne pathogen which can survive in harsh environmental conditions. It responds to external stimuli through an array of two-component systems (TCS) that sense external cues. Several TCS, including LisRK, have been linked to Listeria's ability to grow at slightly elevated antibiotic levels. The aim of this study was to determine if the TCS LisRK is also involved in acquiring the high antibiotic tolerance that is characteristic of persister cells. LisRK activates a response that leads to remodeling of the cell envelope, and we therefore hypothesized that activation of LisRK could also increase in the cells' adhesiveness and initiate the first step in biofilm formation. We used a ΔlisR mutant to study antibiotic tolerance in the presence and absence of LisRK, and a GFP reporter strain to visualize the activation of LisRK in L. monocytogenes LO28 at a single-cell level. LisRK was activated in most cells in stationary phase cultures. Antimicrobial susceptibility tests showed that LisRK was required for the generation of ampicillin tolerance under these conditions. The wildtype strain tolerated exposure to ampicillin at 1,000 × inhibitory levels for 24 h, and the fraction of surviving cells was 20,000-fold higher in the wildtype strain compared to the ΔlisR mutant. The same protection was not offered to other antibiotics (vancomycin, gentamicin, tetracycline), and the mechanism for antibiotic tolerance is thus highly specific. Furthermore, quantification of bacterial attachment rates and attachment force also revealed that the absence of a functional LisRK rendered the cells less adhesive. Hence, LisRK TCS promotes multiple protective mechanisms simultaneously.
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Locally administrable drugs with controllable release on external cues hold great promise for antitumor therapy. Here, we report an injectable, supramolecular hydrogel (SHG), where the drug release can be controllably driven by near infrared (NIR) irradiation. The SHGs are formed by electrostatic interactions with LAPONITE® (XLG), in which upconverting nanoparticles (UCNPs) modified with α-cyclodextrin (α-CD) are used as the core, and azobenzene quaternary ammonium salts (E-azo) are further assembled through host-guest interactions. The hydrogel demonstrates reversible phase transition between gel and sol states and photothermal conversion capability. In detailed in vitro and vivo trials, drug-loaded SHGs successfully suppressed invasion by cancer cells. Phase transitions that are regulated by NIR light and promote drug release using photothermal effects, highlighting the considerable potential of supramolecular hydrogels in anticancer therapies, especially for treatments requiring long-term, on-demand drug supply in clinics.
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Doxorrubicina/química , Portadores de Fármacos/química , Hidrogéis/química , Elementos da Série dos Lantanídeos/química , Substâncias Luminescentes/química , Nanopartículas Metálicas/química , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , Compostos Azo/química , Doxorrubicina/farmacologia , Liberação Controlada de Fármacos , Feminino , Humanos , Raios Infravermelhos , Camundongos Endogâmicos BALB C , Camundongos Nus , Imagem Óptica , Transição de Fase , Terapia Fototérmica , Compostos de Amônio Quaternário/química , Dióxido de Silício/química , Eletricidade Estática , alfa-Ciclodextrinas/químicaRESUMO
LiNbO3 (LN) crystal has been widely used as a pyroelectric material due to its spontaneous electric polarization, which could be recharged easily and can directly convert heat energy into electricity. LN crystal's heat-resistant, low-cost, and low dielectric loss properties make it possible for its applications in room-temperature pyroelectric devices and thermal sensors. However, LN crystal suffers from fragility, inflexibility, and other mechanical properties, which limit its suitability for many applications in various fields. In this study, the LN modified flexible pyroelectric films, composed of LN micro-particles, polypropylene (PP) matrix, and multiwalled carbon nanotubes (MWCNTs), are successfully fabricated. The pyroelectric effects of LN crystal and LN/PP/MWCNT composite films are characterized by monitoring the patterned self-assembly of nanoparticles and the output pyroelectric currents. The excellent pyroelectric properties of the composites have potential applications in energy harvesters or sensors.
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Bioelectricity generation, by Shewanella oneidensis (S. oneidensis) MR-1, has become particularly alluring, thanks to its extraordinary prospects for energy production, pollution treatment, and biosynthesis. Attempts to improve its technological output by modification of S. oneidensis MR-1 remains complicated, expensive and inefficient. Herein, we report on the augmentation of S. oneidensis MR-1 with carbon dots (CDs). The CDs-fed cells show accelerated extracellular electron transfer and metabolic rate, with increased intracellular charge, higher adenosine triphosphate level, quicker substrate consumption and more abundant extracellular secretion. Meanwhile, the CDs promote cellular adhesion, electronegativity, and biofilm formation. In bioelectrical systems the CDs-fed cells increase the maximum current value, 7.34 fold, and power output, 6.46 fold. The enhancement efficacy is found to be strongly dependent on the surface charge of the CDs. This work demonstrates a simple, cost-effective and efficient route to improve bioelectricity generation of S. oneidensis MR-1, holding promise in all relevant technologies.
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Fontes de Energia Bioelétrica , Carbono/metabolismo , Shewanella/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Materiais Biocompatíveis , Biotecnologia , Eletricidade , Técnicas Eletroquímicas , Transporte de Elétrons , Shewanella/genética , Shewanella/ultraestruturaRESUMO
Transition metal selenides (TMSs) with excellent electrochemical activity and high intrinsic electrical conductivity have attracted considerable attention owing to their potential use in energy storage devices. However, the low energy densities of the reported TMSs, which originate from the small active surface area and poor electrolyte ion mobility, substantially restrict their application potential. In this work, porous ultrathin nickel selenide nanosheet networks (NiSe NNs) on nickel foam are fabricated by using a novel, facile method, that is, selenylation/pickling of the pre-formed manganese-doped α-Ni(OH)2 . Removal of Mn resulted in NNs with a highly porous structure. The 3D framework of the NNs and the inherent nature of the NiSe affords high ion mobility, abundant accessible activated sites, vigorous electrochemical activity, and low resistance. One of the highest specific capacities of TMSs ever reported, that is, 443â mA h g-1 (807â µAh cm-2 ) at 3.0â A g-1 , is achieved with the NNs as electrodes. The assembled NiSe NNs//porous carbon hybrid supercapacitor delivers a high energy density of 66.6â Wh kg-1 at a power density of 425â W kg-1 , with excellent cycling stability. This work provides a new strategy for the production of novel electrode materials that can be applied in high-performance hybrid supercapacitors, and a fresh pathway towards commercial applications of hybrid supercapacitors based on TMS electrodes.
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Waterborne health issues continue to grow despite the large number of available solutions. Current sterilization techniques to fight with waterborne diseases struggle to meet the demands on cost, efficiency and reach. Effective alternatives are pressingly required. Here we introduce Prussian blue coated ferroferric oxide (Fe3O4@PB) composites for water sterilization. The composites exhibit superior photothermal inactivation of bacteria under solar-light irradiation, with nearly complete inactivation of bacterial cells in only 15â¯min. Even for the mixed bacteria in authentic water matrices, the composites show excellent bacterial inactivation performance. Moreover, the highly magnetized iron core of the Fe3O4@PB enables magnetic separation and recycling. Multiple cycle runs reveal that Fe3O4@PB composites have exceptional stability and reusability. This work demonstrates a scalable, low-cost, high-efficiency and reusable sterilization method to improve water quality and safety.
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Members of the candidate phylum Acetothermia are globally distributed and detected in various habitats. However, little is known about their physiology and ecological importance. In this study, an operational taxonomic unit belonging to Acetothermia was detected at high abundance in four full-scale anaerobic digesters by 16S rRNA gene amplicon sequencing. The first closed genome from this phylum was obtained by differential coverage binning of metagenomes and scaffolding with long nanopore reads. Genome annotation and metabolic reconstruction suggested an anaerobic chemoheterotrophic lifestyle in which the bacterium obtains energy and carbon via fermentation of peptides, amino acids, and simple sugars to acetate, formate, and hydrogen. The morphology was unusual and composed of a central rod-shaped cell with bipolar prosthecae as revealed by fluorescence in situ hybridization combined with confocal laser scanning microscopy, Raman microspectroscopy, and atomic force microscopy. We hypothesize that these prosthecae allow for increased nutrient uptake by greatly expanding the cell surface area, providing a competitive advantage under nutrient-limited conditions.
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Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Bactérias/metabolismo , DNA Bacteriano , Fermentação , Metagenoma , FilogeniaRESUMO
Finegoldia magna, a Gram-positive anaerobic coccus, is an opportunistic pathogen, associated with medical device-related infections. F. magna is the only described species of the genus Finegoldia. We report the analysis of 17 genomes of Finegoldia isolates. Phylogenomic analyses showed that the Finegoldia population can be divided into two distinct clades, with an average nucleotide identity of 90.7%. One clade contains strains of F. magna, whereas the other clade includes more heterogeneous strains, hereafter tentatively named "Finegoldia nericia". The latter species appears to be more abundant in the human microbiome. Surface structure differences between strains of F. magna and "F. nericia" were detected by microscopy. Strain-specific heterogeneity is high and previously identified host-interacting factors are present only in subsets of "F. nericia" and F. magna strains. However, all genomes encode multiple host factor-binding proteins such as albumin-, collagen-, and immunoglobulin-binding proteins, and two to four copies of CAMP (Christie-Atkins-Munch-Petersen) factors; in accordance, most strains show a positive CAMP reaction for co-hemolysis. Our work sheds new light of the genus Finegoldia and its ability to bind host components. Future research should explore if the genomic differences identified here affect the potential of different Finegoldia species and strains to cause opportunistic infections.
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Heterogeneidade Genética , Genoma Bacteriano , Genótipo , Cocos Gram-Positivos/classificação , Cocos Gram-Positivos/genética , Composição de Bases , Ordem dos Genes , Loci Gênicos , Tamanho do Genoma , Genômica/métodos , Infecções por Bactérias Gram-Positivas/microbiologia , Cocos Gram-Positivos/patogenicidade , Cocos Gram-Positivos/ultraestrutura , Interações Hospedeiro-Patógeno , Filogenia , Fatores de Virulência/genéticaRESUMO
Pathogenic microbes cause infections and are excellent at adapting to the disinfection strategies that address their biochemistry. The growth in number of drug resistant superbugs is alarming, and has attracted attention to alternative solutions. In recent years, researchers have put effort into designing nanomaterials with activatable antimicrobial properties initiated by light irradiation. The underlying mechanisms of these nanomaterials' photoactivatable antimicrobial effect may vary from reactive oxygen species generation, to heat production or pH variation in procedures like photocatalysis, photodynamic therapy, photothermal lysis and photoinduced acidification. In this article, we review the photoactive nanomaterial solutions for fighting against microbial diseases, especially bacterial infections. This Review shines light on the fundamental principles, important developments, promising applications, and limitations of current technologies as well as open questions that these methods may answer or help to answer.
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A spatial arrangement of proteins provides structural and functional advantages in vast technological applications as well as fundamental research. Most protein patterning procedures employ complicated, time consuming and very costly nanofabrication techniques. As an alternative route, we developed a fully biomolecular self-assembly method using DNA Origami Frames (DOF) as a template for both small and large scale protein patterning. We employed a triangular DOF (tDOF) to arrange the Bovine Serum Albumin (BSA) protein. Our in situ protein patterning strategy provides a novel, fully organic platform using a fast and low-cost surface approach with possible utilization in fundamental science and technological applications.
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DNA/química , Conformação Proteica , Soroalbumina Bovina/química , Animais , BovinosRESUMO
The discovery of a microbial ecosystem in ocean sediments has evoked interest in life under extreme energy limitation and its role in global element cycling. However, fundamental parameters such as the size and the amount of biomass of sub-seafloor microbial cells are poorly constrained. Here we determined the volume and the carbon content of microbial cells from a marine sediment drill core retrieved by the Integrated Ocean Drilling Program (IODP), Expedition 347, at Landsort Deep, Baltic Sea. To determine their shape and volume, cells were separated from the sediment matrix by multi-layer density centrifugation and visualized via epifluorescence microscopy (FM) and scanning electron microscopy (SEM). Total cell-carbon was calculated from amino acid-carbon, which was analyzed by high-performance liquid chromatography (HPLC) after cells had been purified by fluorescence-activated cell sorting (FACS). The majority of microbial cells in the sediment have coccoid or slightly elongated morphology. From the sediment surface to the deepest investigated sample (~60 m below the seafloor), the cell volume of both coccoid and elongated cells decreased by an order of magnitude from ~0.05 to 0.005 µm(3). The cell-specific carbon content was 19-31 fg C cell(-1), which is at the lower end of previous estimates that were used for global estimates of microbial biomass. The cell-specific carbon density increased with sediment depth from about 200 to 1000 fg C µm(-3), suggesting that cells decrease their water content and grow small cell sizes as adaptation to the long-term subsistence at very low energy availability in the deep biosphere. We present for the first time depth-related data on the cell volume and carbon content of sedimentary microbial cells buried down to 60 m below the seafloor. Our data enable estimates of volume- and biomass-specific cellular rates of energy metabolism in the deep biosphere and will improve global estimates of microbial biomass.
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MIL-68 (In) nano-rods were prepared by a facile solvothermal synthesis using NaOAc as modulator agent at 100°C for 30 min. The BET test showed that the specific surface area and pore volume of MIL-68 (In) nanorods were 1252 m(2) g(-1) and 0.80 cm(3) g(-1), respectively. The as-prepared MIL-68 (In) nanorods showed excellent adsorption capacity and rapid adsorption rate for removal of Congo red (CR) dye from water. The maximum adsorption capacity of MIL-68 (In) nanorods toward CR reached 1204 mg g(-1), much higher than MIL-68 (In) microrods and most of the previously reported adsorbents. The adsorption process of CR by MIL-68 (In) nano-rods was investigated and found to be obeying the Langmuir adsorption model in addition to pseudo-second-order rate equation. Moreover, the MIL-68 (In) nanorods showed an acceptable reusability after regeneration with ethanol. All information gives an indication that the as-prepared MIL-68 (In) nanorods show their potential as the adsorbent for highly efficient removal of CR in wastewater.
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Corantes/isolamento & purificação , Vermelho Congo/isolamento & purificação , Nanotubos/química , Compostos Organometálicos/química , Águas Residuárias/análise , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos , AdsorçãoRESUMO
BACKGROUND: Exosomes have been implicated in tumour progression and metastatic spread. Little is known of the effect of mechanical and innate immune interactions of malignant cell-derived exosomes on endothelial integrity, which may relate to increased extravasation of circulating tumour cells and, therefore, increased metastatic spread. METHODS: Exosomes isolated from non-malignant immortalized HCV-29 and isogenic malignant non-metastatic T24 and malignant metastatic FL3 bladder cells were characterized by nanoparticle tracking analysis and quantitative nanomechanical mapping atomic force microscopy (QNM AFM) to determine size and nanomechanical properties. Effect of HCV-29, T24 and FL3 exosomes on human umbilical vein endothelial cell (HUVEC) monolayer integrity was determined by transendothelial electrical resistance (TEER) measurements and transport was determined by flow cytometry. Complement activation studies in human serum of malignant and non-malignant cell-derived exosomes were performed. RESULTS: FL3, T24 and HCV-29 cells produced exosomes at similar concentration per cell (6.64, 6.61 and 6.46×10(4) exosomes per cell for FL3, T24 and HCV-29 cells, respectively) and of similar size (120.2 nm for FL3, 127.6 nm for T24 and 117.9 nm for HCV-29, respectively). T24 and FL3 cell-derived exosomes exhibited a markedly reduced stiffness, 95 MPa and 280 MPa, respectively, compared with 1,527 MPa with non-malignant HCV-29 cell-derived exosomes determined by QNM AFM. FL3 and T24 exosomes induced endothelial disruption as measured by a decrease in TEER in HUVEC monolayers, whereas no effect was observed for HCV-29 derived exosomes. FL3 and T24 exosomes traffic more readily (11.6 and 21.4% of applied exosomes, respectively) across HUVEC monolayers than HCV-29 derived exosomes (7.2% of applied exosomes). Malignant cell-derived exosomes activated complement through calcium-sensitive pathways in a concentration-dependent manner. CONCLUSIONS: Malignant (metastatic and non-metastatic) cell line exosomes display a markedly reduced stiffness and adhesion but an increased complement activation compared to non-malignant cell line exosomes, which may explain the observed increased endothelial monolayer disruption and transendothelial transport of these vesicles.
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Production of one dimensional nanomaterials with secondary morphology exhibiting unique functions is challenging. Here we report for the first time that a nanoscale immiscible polymer blend solution electrojet can assemble into ultraporous interweaving microfibers. This intriguingly novel morphology originated from a blend of polycaprolactone (PCL) and polyethylene oxide (PEO) in a DCM-DMF mixed solution when the ratio between each component reached a threshold and when the electrospinning parameters were delicately controlled. The morphology, crystallinity, surface chemistry and wettabilities were characterized to understand the mechanism of formation. The interplay of the two semi-crystalline polymers and the pair of solvents/non-solvents with the electrospinning processing parameters was found to be critical for the formation of the unique structure. Furthermore, the interesting combination of biocompatible, biodegradable PCL with protein-resistant PEO motivated us to assess its inflammation responses on the RAW 264.7 macrophage cell line. All fibers were found to be biocompatible with low inflammation potential upon incubation, while compared with pure PCL nanofibers; the unique interweaving microfibers induced a slightly higher inflammatory reaction.