Assuntos
Antígenos de Diferenciação/biossíntese , Proteínas de Ligação ao Cálcio/biossíntese , Moléculas de Adesão Celular/biossíntese , Rejeição de Enxerto/patologia , Transplante de Rim/imunologia , Macrófagos/patologia , Doença Aguda , Antígenos de Diferenciação/análise , Antígenos de Diferenciação Mielomonocítica/biossíntese , Biomarcadores/análise , Biópsia , Proteínas de Ligação ao Cálcio/análise , Calgranulina A , Calgranulina B , Moléculas de Adesão Celular/análise , Doença Crônica , Quimioterapia Combinada , Selectina E , Rejeição de Enxerto/imunologia , Humanos , Imunossupressores/uso terapêutico , Molécula 1 de Adesão Intercelular/biossíntese , Transplante de Rim/patologia , Macrófagos/imunologia , Molécula-1 de Adesão Celular Endotelial a PlaquetasAssuntos
Moléculas de Adesão Celular/metabolismo , Rejeição de Enxerto/fisiopatologia , Transplante de Rim/fisiologia , Glicoproteínas da Membrana de Plaquetas/metabolismo , Doença Aguda , Anticorpos Monoclonais , Capilares/imunologia , Capilares/patologia , Moléculas de Adesão Celular/análise , Doença Crônica , Endotélio Vascular/imunologia , Endotélio Vascular/patologia , Rejeição de Enxerto/imunologia , Rejeição de Enxerto/patologia , Humanos , Transplante de Rim/imunologia , Transplante de Rim/patologia , Glicoproteínas da Membrana de Plaquetas/análise , Circulação Renal , TrombospondinasRESUMO
Our knowledge of adhesion molecules has exploded over the last 5 years and has swamped most fields of medicine including nephrology. This is not surprising because adhesion molecules play a pivotal role in all aspects of cell to cell contact. Thus, they are involved in important issues, such as fetal development, in any kind of inflammatory or immune response including allograft rejection, as well as thrombus formation, and in tumor growth and metastasis (1-3). This short overview briefly reports some aspects of the biology of relevant adhesion molecules and their significance in inflammatory kidney diseases and in hemodialysis and renal allograft rejection. Finally, new therapeutic opportunities that arise by blocking adhesion molecule function are discussed.
Assuntos
Selectina E/biossíntese , Molécula 1 de Adesão Intercelular/biossíntese , Nefropatias/metabolismo , Molécula-1 de Adesão Celular Endotelial a Plaquetas/biossíntese , Molécula 1 de Adesão de Célula Vascular/biossíntese , Selectina E/efeitos adversos , Selectina E/sangue , Glomerulonefrite/metabolismo , Glomerulonefrite/terapia , Rejeição de Enxerto/metabolismo , Rejeição de Enxerto/terapia , Humanos , Molécula 1 de Adesão Intercelular/efeitos adversos , Molécula 1 de Adesão Intercelular/sangue , Transplante de Rim , Neutropenia/etiologia , Molécula-1 de Adesão Celular Endotelial a Plaquetas/efeitos adversos , Molécula-1 de Adesão Celular Endotelial a Plaquetas/sangue , Diálise Renal/efeitos adversos , Regulação para Cima , Molécula 1 de Adesão de Célula Vascular/efeitos adversos , Molécula 1 de Adesão de Célula Vascular/sangueRESUMO
Recently, we have demonstrated that acute cellular rejection is correlated with a massive infiltration of 27E10-positive macrophages. To examine the distribution of macrophage differentiation markers in the infiltrate in the very early post-transplantation period, two biopsies were taken intraoperatively, approximately 1 h following reperfusion, in each of 16 renal transplant recipients. One biopsy was taken for conventional histology and the other biopsy was snap-frozen. The sections were stained using an ABC indirect immunoperoxidase technique. A panel of monoclonal antibodies against three macrophage differentiation markers (27E10, 25F9 and RM3/1) was used to stain the sections. Using the early inflammation macrophage marker 27E10, there was an unexpected strong staining in 3 out of 16 biopsies. This severe infiltration of 27E10-positive macrophages with 10-20 macrophages per high power field (compared to 0-2 in others) was correlated in all cases with a poor outcome of the graft. All seven kidneys with no 27E10-positive infiltration showed a good function 6 weeks post-transplantation. The other macrophage markers, 25F9 and RM3/1, showed a less marked correlation with graft outcome. In conclusion, a massive infiltration of renal allografts with 27E10-positive macrophages 1 h post-transplantation may be a very early predictor of poor graft outcome.