RESUMO
It has been demonstrated that ß-endorphin stimulates the zymosan-induced secretion of reactive oxygen species and suppresses the spontaneous production of IL-1ß and IL-10 by murine peritoneal macrophages in vivo.
Assuntos
Interleucina-10/biossíntese , Interleucina-1beta/biossíntese , Macrófagos Peritoneais/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Fator de Necrose Tumoral alfa/biossíntese , beta-Endorfina/farmacologia , Animais , CamundongosRESUMO
Blockade of δ-receptors with naltrindole under conditions of systemic immunization abolished the stimulatory effect of ß-endorphin (0.0005 µg/kg) on the counts of antibody-producing cells and the titer of antierythrocyte antibodies. Injection of ß-endorphin to mice led to stimulation of concanavalin A-induced proliferative activity of splenocytes and IL-4 secretion by the naloxone-dependent mechanism. The peptide did not modify the production of IL-2 and IFN-γ.
Assuntos
Receptores Opioides delta/metabolismo , Baço/citologia , beta-Endorfina/farmacologia , Animais , Concanavalina A/farmacologia , Interferon gama/metabolismo , Interleucina-2/metabolismo , Interleucina-4/metabolismo , Masculino , Camundongos , Naloxona/farmacologia , Naltrexona/análogos & derivados , Naltrexona/farmacologia , Receptores Opioides delta/antagonistas & inibidores , Baço/efeitos dos fármacosRESUMO
Glycolipid biosurfactant synthesized by nonpathogenic strain Rhodococcus ruber IEGM231 modulated the production of ROS and IL-8 by peripheral blood neutrophils in spontaneous and stimulated cultures. Secretion of IL-1ß Ð¸ TNF-α by neutrophils in the presence of biosurfactant changed insignificantly.
Assuntos
Glicolipídeos/farmacologia , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Rhodococcus/química , Tensoativos/farmacologia , Células Cultivadas , Glicolipídeos/química , Humanos , Interleucina-8/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Fator de Necrose Tumoral alfa/metabolismoRESUMO
Intraperitoneal injection of ß-endorphin in doses of 1, 0.01, and 0.0005 µg/kg under conditions of systemic immunization increased the count of antibody-producing cells in the spleen and the titer of anti-erythrocyte antibodies in the plasma of experimental animals. Intraperitoneal ß-endorphin stimulated proliferative activity of splenocytes in mice in the presence of both B- and T-cell mitogen, did not change the production of IFN-γ, reduced the level of IL-2, and stimulated the secretion of IL-4, the main Th2-polarizing factor.
Assuntos
Neurotransmissores/administração & dosagem , Células Th1/efeitos dos fármacos , Equilíbrio Th1-Th2/efeitos dos fármacos , Células Th2/efeitos dos fármacos , beta-Endorfina/administração & dosagem , Animais , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Concanavalina A/farmacologia , Relação Dose-Resposta a Droga , Imunidade Humoral , Injeções Intraperitoneais , Interferon gama/biossíntese , Interferon gama/imunologia , Interleucina-2/biossíntese , Interleucina-2/imunologia , Interleucina-4/biossíntese , Interleucina-4/imunologia , Lipopolissacarídeos/farmacologia , Ativação Linfocitária/efeitos dos fármacos , Ativação Linfocitária/imunologia , Masculino , Camundongos , Baço/citologia , Baço/efeitos dos fármacos , Baço/imunologia , Células Th1/citologia , Células Th1/imunologia , Células Th2/citologia , Células Th2/imunologiaRESUMO
Endogenous opioid peptides comprise a group of bioregulatory factors involved in regulation of functional activity of various physiological systems of an organism. One of most important functions of endogenous opioids is their involvement in the interaction between cells of the nervous and immune systems. Summary data on the effects of opioid peptides on regulation of functions of innate immunity cells are presented.
Assuntos
Sistema Imunitário/citologia , Sistema Imunitário/imunologia , Imunidade Inata , Peptídeos Opioides/imunologia , Sequência de Aminoácidos , Animais , Humanos , Sistema Imunitário/metabolismo , Dados de Sequência Molecular , Peptídeos Opioides/química , Receptores Opioides/metabolismo , Transdução de Sinais/imunologia , Estresse Fisiológico/imunologiaRESUMO
The glycolipid biosurfactant complex from Rhodococcus ruber IEGM 231 had a stimulatory effect on the production of IL-12, IL-18, and reactive oxygen species by cells of the innate immunity. This effect depended on the composition of cell cultures and presence of LPS. It was primarily observed in non-stimulated cultures. The glycolipid biosurfactant complex had little effect on IL-10 secretion by monocytes and mononuclear cells.
Assuntos
Citocinas/metabolismo , Imunidade Inata , Oxirredução , Rhodococcus/fisiologia , Tensoativos/farmacologiaAssuntos
Ativação Linfocitária/efeitos dos fármacos , Monócitos/fisiologia , Receptores Opioides delta/agonistas , Receptores Opioides mu/agonistas , beta-Endorfina/farmacologia , Adulto , Proliferação de Células , Ala(2)-MePhe(4)-Gly(5)-Encefalina/farmacologia , Leucina Encefalina-2-Alanina/farmacologia , Humanos , Linfócitos/imunologia , Masculino , Naloxona , Neurotransmissores/farmacologia , Receptores Opioides delta/antagonistas & inibidores , Receptores Opioides mu/antagonistas & inibidores , alfa-MSH/farmacologiaRESUMO
The biosurfactant glycolipid complex synthesized by Rhodococcus ruber actinobacteria is not toxic and exhibits no appreciable effect on proliferative activity of peripheral blood leukocytes. In the monocyte fraction, the biosurfactant activates the production of IL-1beta and TNF-alpha cytokines without modifying the production of IL-6. In the mononuclear fraction, the glycolipid biosurfactant exhibited no effects on the production of IL-1beta, TNF-alpha, and IL-6. These results indicate good prospects for further studies of immunomodulating and antitumor activities of biosurfactant drug.
Assuntos
Glicolipídeos/imunologia , Fatores Imunológicos/imunologia , Rhodococcus/química , Tensoativos/metabolismo , Humanos , Interleucina-1beta/imunologia , Interleucina-6/imunologia , Leucócitos/citologia , Leucócitos/imunologia , Monócitos/citologia , Monócitos/imunologia , Fator de Necrose Tumoral alfa/imunologiaRESUMO
beta-Endorphin in vivo produced different effects on antibody production depending on the administered dose. Blockade of opioid receptors with naloxone (but not naltrindole) abolished the effects of beta-endorphin. The peptide activated IL-4 production in vitro via activation of delta-opioid receptors.