RESUMO
In the original version of this Data Descriptor the word "Gulf" was incorrectly spelled in the affiliation "Ocean College, Beibu Gulf University, Qinzhou, 535011, Guangxi, China". This has now been corrected in both the HTML and PDF versions.
RESUMO
Chinese horseshoe crabs (Tachypleus tridentatus), ancient marine arthropods dating back to the mid-Palaeozoic Era, have provided valuable resources for the detection of bacterial or fungal contamination. However, excessive exploitation for the amoebocyte lysate of Tachypleus has dramatically decreased the population of the Chinese horseshoe crabs. Thus, we present sequencing, assembly and annotation of T. tridentatus, with the hope of understanding the genomic feature of the living fossil and assisting scientists with the protection of this endangered species. The final genome contained a total size of 1.943 Gb, covering 90.23% of the estimated genome size. The transcriptome of three larval stages was constructed to investigate the candidate gene involved in the larval development and validate annotation. The completeness of the genome and gene models was estimated by BUSCO, reaching 96.2% and 95.4%, respectively. The synonymous substitution distribution of paralogues revealed that T. tridentatus had undergone two rounds of whole-genome duplication. All genomic and transcriptome data have been deposited in public databases, ready to be used by researchers working on horseshoe crabs.
Assuntos
Genoma , Caranguejos Ferradura/genética , Transcriptoma , Animais , Espécies em Perigo de Extinção , Anotação de Sequência MolecularRESUMO
After seedlings of Aeluropus sinensis var. sinensis Debeaux were treated with different NaCl concentrations (0-200 mmol/L) for 14 days, some physiological indexes were measured. The higher the NaCl concentration, the more the growth of A. sinensis was inhibited. The increase in root/shoot ratio suggests that the shoots are more sensitive to salinity than the roots. The diminished leaf area may reduce the transpiration rate, and the root mainly grew longitudinally, which may help the root to reach the water source under the high salinity conditions. Chl a contents increased, so did Chl b, but the Chl a/Chl b ratio declined, which implies the stimulation of Chl a accepted from NaCl is smaller than that of Chl b. After salt treatment, the organic solute contents increased (P<0.05), the proline, amino acids and soluble sugar contents increased more than organic acids, the increase in soluble carbohydrate may inhibit photosynthesis in feedback. The percentage of sucrose in soluble carbohydrates increased too. Although the organic dry weight of whole plant declined, the proportion of organic dry weight in total dry weight increased, and the osmotic potential of plant cell juice declined, which implies that the contribution of organic matter to osmotic adjustment increased with salinity. That is, A. sinensis had ability to tolerate salinity to a certain degree.