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European forests are threatened by increasing numbers of invasive pests and pathogens. Over the past century, Lecanosticta acicola, a foliar pathogen predominantly of Pinus spp., has expanded its range globally, and is increasing in impact. Lecanosticta acicola causes brown spot needle blight, resulting in premature defoliation, reduced growth, and mortality in some hosts. Originating from southern regions of North American, it devastated forests in the USA's southern states in the early twentieth century, and in 1942 was discovered in Spain. Derived from Euphresco project 'Brownspotrisk,' this study aimed to establish the current distribution of Lecanosticta species, and assess the risks of L. acicola to European forests. Pathogen reports from the literature, and new/ unpublished survey data were combined into an open-access geo-database (http://www.portalofforestpathology.com), and used to visualise the pathogen's range, infer its climatic tolerance, and update its host range. Lecanosticta species have now been recorded in 44 countries, mostly in the northern hemisphere. The type species, L. acicola, has increased its range in recent years, and is present in 24 out of the 26 European countries where data were available. Other species of Lecanosticta are largely restricted to Mexico and Central America, and recently Colombia. The geo-database records demonstrate that L. acicola tolerates a wide range of climates across the northern hemisphere, and indicate its potential to colonise Pinus spp. forests across large swathes of the Europe. Preliminary analyses suggest L. acicola could affect 62% of global Pinus species area by the end of this century, under climate change predictions. Although its host range appears slightly narrower than the similar Dothistroma species, Lecanosticta species were recorded on 70 host taxa, mostly Pinus spp., but including, Cedrus and Picea spp. Twenty-three, including species of critical ecological, environmental and economic significance in Europe, are highly susceptible to L. acicola, suffering heavy defoliation and sometimes mortality. Variation in apparent susceptibility between reports could reflect variation between regions in the hosts' genetic make-up, but could also reflect the significant variation in L. acicola populations and lineages found across Europe. This study served to highlight significant gaps in our understanding of the pathogen's behaviour. Lecanosticta acicola has recently been downgraded from an A1 quarantine pest to a regulated non quarantine pathogen, and is now widely distributed across Europe. With a need to consider disease management, this study also explored global BSNB strategies, and used Case Studies to summarise the tactics employed to date in Europe.
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BACKGROUND: Limited information is available about patterns of surgical management of early breast cancer by ethnicity of women in England, and any potential inequalities in the treatment received for breast cancer. METHODS: National Cancer Registration and Analysis Service data for women diagnosed with early invasive breast cancer (ICD-10 C50) during 2012-2017 were analysed. Multivariable logistic regression was used to estimate odds ratios (ORs) and 95 per cent confidence intervals for the risk of mastectomy versus breast-conserving surgery by ethnicity (black African, black Caribbean, Indian, Pakistani and white), adjusting for age, region, deprivation, year of diagnosis, co-morbidity and stage at diagnosis. RESULTS: Data from 164 143 women were included in the analysis. The proportion of women undergoing mastectomy fell by approximately 5 per cent between 2012 and 2017 across all the ethnic groups examined. In unadjusted analyses, each ethnic minority group had a significantly higher odds of mastectomy than white women; however, in the fully adjusted model, there were no significantly increased odds of having mastectomy for women of any ethnic minority group examined. For example, compared with white women, the unadjusted and fully adjusted ORs for mastectomy were 1·14 (95 per cent c.i. 1·05 to 1·20) and 1·04 (0·96 to 1·14) respectively for Indian women, and 1·45 (1·30 to 1·62) and 1·00 (0·89 to 1·13) for black African women. This attenuation in OR by ethnicity was largely due to adjustment for age and stage. CONCLUSION: Allowing for different patterns of age and stage at presentation, the surgical management of early breast cancer is similar in all women, regardless of ethnicity.
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Neoplasias da Mama/cirurgia , Carcinoma Ductal de Mama/cirurgia , Mastectomia/estatística & dados numéricos , Grupos Raciais/estatística & dados numéricos , Adulto , Distribuição por Idade , Idoso , Idoso de 80 Anos ou mais , Neoplasias da Mama/patologia , Carcinoma in Situ/patologia , Carcinoma in Situ/cirurgia , Carcinoma Ductal de Mama/patologia , Detecção Precoce de Câncer , Inglaterra/epidemiologia , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
The genus Calonectria includes many important plant pathogens with a wide global distribution. In order to better understand the reproductive biology of these fungi, we characterised the structure of the mating type locus and flanking genes using the genome sequences for seven Calonectria species. Primers to amplify the mating type genes in other species were also developed. PCR amplification of the mating type genes and multi-gene phylogenetic analyses were used to investigate the mating strategies and evolution of mating type in a collection of 70 Calonectria species residing in 10 Calonectria species complexes. Results showed that the organisation of the MAT locus and flanking genes is conserved. In heterothallic species, a novel MAT gene, MAT1-2-12 was identified in the MAT1-2 idiomorph; the MAT1-1 idiomorph, in most cases, contained the MAT1-1-3 gene. Neither MAT1-1-3 nor MAT1-2-12 was found in homothallic Calonectria (Ca.) hongkongensis, Ca. lateralis, Ca. pseudoturangicola and Ca. turangicola. Four different homothallic MAT locus gene arrangements were observed. Ancestral state reconstruction analysis provided evidence that the homothallic state was basal in Calonectria and this evolved from a heterothallic ancestor.
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The native 'ohi'a lehua (Metrosideros polymorpha) has cultural, biological and ecological significance to Hawai'i, but it is seriously threatened by a disease commonly referred to as rapid 'ohi'a death (ROD). Preliminary investigations showed that a Ceratocystis species similar to C. fimbriata s.lat. was the cause of the disease. In this study, we used a combination of the phylogenetic, morphological and biological species concepts, as well as pathogenicity tests and microsatellite analyses, to characterise isolates collected from diseased 'ohi'a trees across Hawai'i Island. Two distinct lineages, representing new species of Ceratocystis, were evident based on multigene phylogenetic analyses. These are described here as C. lukuohia and C. huliohia. Ceratocystis lukuohia forms part of the Latin American clade (LAC) and was most closely associated with isolates from Syngonium and Xanthosoma from the Caribbean and elsewhere, including Hawai'i, and C. platani, which is native to eastern USA. Ceratocystis huliohia resides in the Asian-Australian clade (AAC) and is most closely related to C. uchidae, C. changhui and C. cercfabiensis, which are thought to be native to Asia. Morphology and interfertility tests support the delineation of these two new species and pathogenicity tests show that both species are aggressive pathogens on seedlings of M. polymorpha. Characterisation of isolates using microsatellite markers suggest that both species are clonal and likely represent recently-introduced strains. Intensive research is underway to develop rapid screening protocols for early detection of the pathogens and management strategies in an attempt to prevent the spread of the pathogens to the other islands of Hawai'i, which are currently disease free.
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BACKGROUND: There is limited information about participation in organised population-wide screening programmes by people with disabilities. METHODS: Data from the National Health Service routine screening programmes in England were linked to information on disability reported by the Million Women Study cohort participants. RESULTS: Of the 473 185 women offered routine breast or bowel cancer screening, 23% reported some disability. Women with disabilities were less likely than other women to participate in breast cancer screening (RR=0.64, 95% CI: 0.62-0.65) and in bowel cancer screening (RR=0.75, 0.73-0.76). Difficulties with self-care or vision were associated with the greatest reduction in screening participation. CONCLUSION: Participation in routine cancer screening programmes in England is reduced in people with disabilities and participation varies by type of disability.
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Neoplasias da Mama/diagnóstico , Neoplasias Colorretais/diagnóstico , Pessoas com Deficiência , Detecção Precoce de Câncer/estatística & dados numéricos , Participação do Paciente , Idoso , Inglaterra , Feminino , Humanos , Pessoa de Meia-Idade , Estudos ProspectivosRESUMO
Genera of Phytopathogenic Fungi (GOPHY) is introduced as a new series of publications in order to provide a stable platform for the taxonomy of phytopathogenic fungi. This first paper focuses on 21 genera of phytopathogenic fungi: Bipolaris, Boeremia, Calonectria, Ceratocystis, Cladosporium, Colletotrichum, Coniella, Curvularia, Monilinia, Neofabraea, Neofusicoccum, Pilidium, Pleiochaeta, Plenodomus, Protostegia, Pseudopyricularia, Puccinia, Saccharata, Thyrostroma, Venturia and Wilsonomyces. For each genus, a morphological description and information about its pathology, distribution, hosts and disease symptoms are provided. In addition, this information is linked to primary and secondary DNA barcodes of the presently accepted species, and relevant literature. Moreover, several novelties are introduced, i.e. new genera, species and combinations, and neo-, lecto- and epitypes designated to provide a stable taxonomy. This first paper includes one new genus, 26 new species, ten new combinations, and four typifications of older names.
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BACKGROUND: Anal cancer incidence increases with age and is higher in women than men. Risk factors in this group other than high-risk human papillomavirus infection are unclear. METHODS: In all, 1.3 million women were recruited in 1996-2001 and followed for incident anal cancer. Cox regression models were used to calculate relative risks (RRs) for anal cancer by various potential risk factors. RESULTS: Five hundred and seventeen incident anal cancers were registered over 13 years of follow-up. The largest RR was associated with a history of cervical intraepithelial neoplasia grade 3 (CIN 3; RR=4.03, 95% CI 2.59-6.28). Other factors associated with significantly increased risks in multivariate analyses were: ever smoking (RR=1.49, 1.24-1.80); previous use of oral contraceptives (RR=1.51, 1.24-1.83); nulliparity (RR=1.61, 1.24-2.07); tubal ligation (RR=1.39, 1.13-1.70) and not living with a partner (RR=1.82, 1.40-2.38). The association with smoking was significantly greater for squamous cell carcinoma than adenocarcinoma of the anus (RR 1.66 vs 0.89, P for heterogeneity=0.04). CONCLUSIONS: History of CIN 3, smoking, past oral contraceptive use, nulliparity, tubal ligation and not living with a partner are risk factors for anal cancer in women. There was a significant increase in risk associated with smoking for squamous cell anal cancers but not adenocarcinomas.
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Adenocarcinoma/epidemiologia , Neoplasias do Ânus/epidemiologia , Carcinoma de Células Escamosas/epidemiologia , Estilo de Vida , Infecções por Papillomavirus/complicações , Displasia do Colo do Útero/complicações , Neoplasias do Colo do Útero/complicações , Adenocarcinoma/virologia , Adulto , Idoso , Neoplasias do Ânus/virologia , Carcinoma de Células Escamosas/virologia , Inglaterra/epidemiologia , Feminino , Seguimentos , Humanos , Incidência , Pessoa de Meia-Idade , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/transmissão , Infecções por Papillomavirus/virologia , Prognóstico , Estudos Prospectivos , História Reprodutiva , Fatores de Risco , Neoplasias do Colo do Útero/virologia , Displasia do Colo do Útero/virologiaRESUMO
Novel species of fungi described in the present study include the following from Malaysia: Castanediella eucalypti from Eucalyptus pellita, Codinaea acacia from Acacia mangium, Emarcea eucalyptigena from Eucalyptus brassiana, Myrtapenidiella eucalyptorum from Eucalyptus pellita, Pilidiella eucalyptigena from Eucalyptus brassiana and Strelitziana malaysiana from Acacia mangium. Furthermore, Stachybotrys sansevieriicola is described from Sansevieria ehrenbergii (Tanzania), Phacidium grevilleae from Grevillea robusta (Uganda), Graphium jumulu from Adansonia gregorii and Ophiostoma eucalyptigena from Eucalyptus marginata (Australia), Pleurophoma ossicola from bone and Plectosphaerella populi from Populus nigra (Germany), Colletotrichum neosansevieriae from Sansevieria trifasciata, Elsinoë othonnae from Othonna quinquedentata and Zeloasperisporium cliviae (Zeloasperisporiaceae fam. nov.) from Clivia sp. (South Africa), Neodevriesia pakbiae, Phaeophleospora hymenocallidis and Phaeophleospora hymenocallidicola on leaves of a fern (Thailand), Melanconium elaeidicola from Elaeis guineensis (Indonesia), Hormonema viticola from Vitis vinifera (Canary Islands), Chlorophyllum pseudoglobossum from a grassland (India), Triadelphia disseminata from an immunocompromised patient (Saudi Arabia), Colletotrichum abscissum from Citrus (Brazil), Polyschema sclerotigenum and Phialemonium limoniforme from human patients (USA), Cadophora vitícola from Vitis vinifera (Spain), Entoloma flavovelutinum and Bolbitius aurantiorugosus from soil (Vietnam), Rhizopogon granuloflavus from soil (Cape Verde Islands), Tulasnella eremophila from Euphorbia officinarum subsp. echinus (Morocco), Verrucostoma martinicensis from Danaea elliptica (French West Indies), Metschnikowia colchici from Colchicum autumnale (Bulgaria), Thelebolus microcarpus from soil (Argentina) and Ceratocystis adelpha from Theobroma cacao (Ecuador). Myrmecridium iridis (Myrmecridiales ord. nov., Myrmecridiaceae fam. nov.) is also described from Iris sp. (The Netherlands). Novel genera include (Ascomycetes): Budhanggurabania from Cynodon dactylon (Australia), Soloacrosporiella, Xenocamarosporium, Neostrelitziana and Castanediella from Acacia mangium and Sabahriopsis from Eucalyptus brassiana (Malaysia), Readerielliopsis from basidiomata of Fuscoporia wahlbergii (French Guyana), Neoplatysporoides from Aloe ferox (Tanzania), Wojnowiciella, Chrysofolia and Neoeriomycopsis from Eucalyptus (Colombia), Neophaeomoniella from Eucalyptus globulus (USA), Pseudophaeomoniella from Olea europaea (Italy), Paraphaeomoniella from Encephalartos altensteinii, Aequabiliella, Celerioriella and Minutiella from Prunus (South Africa). Tephrocybella (Basidiomycetes) represents a novel genus from wood (Italy). Morphological and culture characteristics along with ITS DNA barcodes are provided for all taxa.
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The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Amplification efficiencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across > 1 500 species (1 931 strains or specimens) and the outcomes of almost twenty thousand (19 577) polymerase chain reactions were evaluated. We tested several well-known primer pairs that amplify: i) sections of the nuclear ribosomal RNA gene large subunit (D1-D2 domains of 26/28S); ii) the complete internal transcribed spacer region (ITS1/2); iii) partial ß -tubulin II (TUB2); iv) γ-actin (ACT); v) translation elongation factor 1-α (TEF1α); and vi) the second largest subunit of RNA-polymerase II (partial RPB2, section 5-6). Their PCR efficiencies were compared with novel candidate primers corresponding to: i) the fungal-specific translation elongation factor 3 (TEF3); ii) a small ribosomal protein necessary for t-RNA docking; iii) the 60S L10 (L1) RP; iv) DNA topoisomerase I (TOPI); v) phosphoglycerate kinase (PGK); vi) hypothetical protein LNS2; and vii) alternative sections of TEF1α. Results showed that several gene sections are accessible to universal primers (or primers universal for phyla) yielding a single PCR-product. Barcode gap and multi-dimensional scaling analyses revealed that some of the tested candidate markers have universal properties providing adequate infra- and inter-specific variation that make them attractive barcodes for species identification. Among these gene sections, a novel high fidelity primer pair for TEF1α, already widely used as a phylogenetic marker in mycology, has potential as a supplementary DNA barcode with superior resolution to ITS. Both TOPI and PGK show promise for the Ascomycota, while TOPI and LNS2 are attractive for the Pucciniomycotina, for which universal primers for ribosomal subunits often fail.
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BACKGROUND: Thyroid cancer incidence is increasing worldwide, but with large variations in incidence that may reflect either diagnostic bias or true ethnic differences. We sought to determine the effect of ethnicity on the incidence of thyroid cancer in England, a multiethnic population with a single health-care system. METHODS: We analysed 11,263 thyroid cancer registrations with ethnicity obtained by linkage to the Hospital Episodes Statistics database. Incidence rate ratios (RRs) adjusted for age, sex and income were calculated for the six main non-White ethnic groups in England compared with Whites and to each other. RESULTS: Thyroid cancer incidence was higher in all ethnic groups, except Indians, compared with Whites: in Pakistanis (RR 1.79, 99% floating confidence interval (FCI) 1.47-2.19); Bangladeshis (RR 1.99, 99% FCI 1.46-2.71); Black Africans (RR 1.69, 99% FCI 1.34-2.13); Black Caribbeans (RR 1.56, 99% FCI 1.25-1.93); and Chinese (RR 2.14, 99% FCI 1.63-2.80). CONCLUSION: The risk of thyroid cancer in England varies significantly by ethnicity. The elevated incidence in most ethnic minorities is unlikely to be due to diagnostic bias and warrants further investigation.
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Neoplasias da Glândula Tireoide/etnologia , Neoplasias da Glândula Tireoide/epidemiologia , Inglaterra/epidemiologia , Etnicidade , Feminino , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , RiscoRESUMO
The genus Ceratocystis was established in 1890 and accommodates many important fungi. These include serious plant pathogens, significant insect symbionts and agents of timber degradation that result in substantial economic losses. Virtually since its type was described from sweet potatoes, the taxonomy of Ceratocystis has been confused and vigorously debated. In recent years, particulary during the last two decades, it has become very obvious that this genus includes a wide diversity of very different fungi. These have been roughly lumped together due to their similar morphological structures that have clearly evolved through convergent evolution linked to an insect-associated ecology. As has been true for many other groups of fungi, the emergence of DNA-based sequence data and associated phylogenetic inferences, have made it possible to robustly support very distinct boundaries defined by morphological characters and ecological differences. In this study, DNA-sequence data for three carefully selected gene regions (60S, LSU, MCM7) were generated for 79 species residing in the aggregate genus Ceratocystis sensu lato and these data were subjected to rigorous phylogenetic analyses. The results made it possible to distinguish seven major groups for which generic names have been chosen and descriptions either provided or emended. The emended genera included Ceratocystis sensu stricto, Chalaropsis, Endoconidiophora, Thielaviopsis, and Ambrosiella, while two new genera, Davidsoniella and Huntiella, were described. In total, 30 new combinations have been made. This major revision of the generic boundaries in the Ceratocystidaceae will simplify future treatments and work with an important group of fungi including distantly related species illogically aggregated under a single name.
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During 2010 and 2011, Dothistroma needle blight (DNB), also known as red band needle blight, was observed for the first time in Cass and Pembina counties in North Dakota (ND). In Pembina Co., DNB was observed in two sites in the Jay V. Wessels Wildlife Management Area (JWWMA). In September 2009, yellow spots on green needles were observed on some trees along the western edge of one planting. By June 2010, DNB was found on third- and fourth-year needles in both JWWMA plantings. Symptoms had developed into dark brown bands or spots on necrotic needles that contained erumpent black acervuli. In June 2011, similar DNB symptoms were observed on Pinus nigra, P. flexilis, P. ponderosa, P. cembra, and P. albicaulis in the Dale E. Herman Research Arboretum, Cass Co., ND. DNB was collected in July 2011 in Brookings Co., South Dakota (SD), from a seed source provenance planting of P. ponderosa. To identify the species causing the infections, symptomatic needles were collected in 2010 from both sites in JWWMA and then again from all four locations in 2011 on all pine species infected. Needles of P. nigra from a private residence near Fairland in Shelby County, Indiana (IN), were also included in the sample set. The rDNA-ITS was PCR-amplified either directly from conidia obtained from acervuli on the needles or from cultures obtained from isolations. Amplicons were sequenced and a BLAST search was performed in GenBank. The sequences of samples obtained from P. nigra, P. flexilis, P. cembra, and P. albicaulis in ND, P. ponderosa in SD, and P. nigra from IN showed 100% sequence homology with Dothistroma pini (Accession No. AY808302). These isolates were identical to all previously assayed isolates of D. pini from Nebraska, Minnesota, and Michigan in the United States. The P. ponderosa isolates from all three sites in ND differed from the other isolates and contained a 1-bp point mutation from a C to a T at site 72 (sequence deposited in GenBank, accession KJ933441). Mating type was determined using species-specific mating type primers for D. pini (3). All 26 samples from ND and SD were of the MAT-1 idiomorph, while the sample from IN contained the MAT-2 idiomorph. All cultures are maintained at FABI, University of Pretoria, South Africa. The two species that cause DNB, D. septosporum (G. Dorog.) M. Morelet and D. pini Hulbary, are morphologically indistinguishable and molecular characterization remains essential for correct species identification (1). Host and geographical distribution range determinations of Dothistroma spp. made without molecular methods are not valid. To date, species confirmed using DNA sequences in the United States include D. septosporum in the Pacific Northwest states of Oregon and Idaho on P. ponderosa, in Montana on P. contorta v. latifolia, and D. pini in the North Central states of Nebraska, Minnesota, and Michigan on P. nigra (1). This study documents the presence of D. pini in three additional states, including a first report of DNB in ND and SD. It also includes new records of D. pini infecting P. flexilis, P. cembra, P. albicaulis, and P. ponderosa. Results of this study have expanded the documented host range of D. pini in the United States from one (P. nigra) to five species. Globally, D. pini is now known to infect a total of 10 pine hosts (2,4,5). References: (1) I. Barnes et al. Stud. Mycol. 50:551, 2004. (2) I. Barnes et al. For. Pathol. 41:361, 2011. (3) M. Groenewald et al. Phytopathology 97:825, 2007. (4) D. Piou et al. Plant Dis. 98:841, 2014. (5) B. Piskur et al. For. Pathol. 43:518, 2013.
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Product analyses of the NO3 radical-initiated oxidation of ortho-, meta-, and para-cresol have been performed in large-volume chamber systems at the University of Wuppertal (1080 L quartz glass reactor: QUAREC) and the European Photoreactor (EUPHORE), Valencia, Spain. The reaction of O3 with NO2 was used for the in situ generation of NO3 radicals in both QUAREC and EUPHORE. In the QUAREC experiments the gas-phase reaction of ortho-cresol isomer with NO3 yielded (11.5 ± 0.8) % 6-methyl-2-nitrophenol (6M2NP), (4.4 ± 0.3) % methyl-1,4-benzoquinone (MQUIN) and (77.2 ± 6.3) % HNO3. The reaction of NO3 radicals with meta-cresol yielded (21.2 ± 1.4) % 3-methyl-2-nitrophenol (3M2NP), (22.8 ± 1.8) % 3-methyl-4-nitrophenol (3M4NP), (23.5 ± 1.8) % 5-methyl-2-nitrophenol (5M2NP), (4.2 ± 0.7) % MQUIN and (72.3 ± 6.4) % HNO3. In the reaction of NO3 radicals with para-cresol, 4-methyl-2-nitrophenol (4M2NP) and HNO3 were identified as products with yields of (41.3 ± 3.7) % and (85.0 ± 10.2) %, respectively. In the EUPHORE chamber not all products were formed at levels above the detection limit, however, in cases where detection was possible similar product yields were observed. The product formation yields determined in both chambers are compared with available literature data and a gas-phase mechanism is proposed to explain the formation of the products observed from the reaction of NO3 and with cresol isomers.
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Cresóis/química , Nitratos/química , Isomerismo , Limite de Detecção , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Dothistroma needle blight (DNB) is caused by two ascomycete fungi, Dothistroma septosporum with a worldwide distribution and D. pini found in the United States, Russia, Ukraine, Hungary, and France (1). DNB has been known in Greece since the early 1980s (3) and the species responsible for the disease was reported as D. pini. In December 2011, needles were collected from three trees in Lagada, Thessaloniki regional unit of Central Macedonia (northern Greece), where the disease was first recorded. DNB infection seems to be limited to a valley in this area in Pinus nigra and P. brutia plantations established more than 50 years ago in an originally deciduous oak forest. Infections were observed over an area of 50 to 60 ha of pine plantations. Although the majority of pine trees were infected by the pathogen, the severity of the disease was relatively low and mortality of infected trees was not observed. Infections were limited to the lower branches in the 50-year-old trees, while on a limited number of younger trees of P. brutia (10 to 15 years old), infection was more severe, extending to the entire crowns of the trees. DNB does not appear to be very common in Greece. Infected needles had reddish-brown bands, usually with necrotic tips or entirely necrotic needles. Black sub-epidermal fruiting bodies (acervuli) were observed with the needle epidermis split and raised. Isolations were made from fruiting structures on needles of P. nigra and P. brutia (50-year-old trees) and P. brutia (10-year-old trees) after surface disinfection with 70% ethanol. Conidia from single fruiting bodies were transferred onto 3% malt extract agar (MEA) in petri dishes and incubated at 20°C. Colonies on MEA had a radial growth rate of 1.3 to 1.6 mm per week, were crustose, brown to grey-brown, and partly covered with slimy masses of conidia. The agar surrounding the colonies had a reddish color. Conidia from acervuli on the needles and the cultures were similar in shape and size, filiform, hyaline, 2 to 4 septate, and 1.8 to 2.5 (3.3) × 22 to 47 µm long. DNA was extracted from three cultures: one from P. nigra (CMW 37966) and two from P. brutia (CMW 37965, CMW 37967) using a standard phenol/chloroform method. The internal transcribed spacer (ITS) region was amplified and sequenced (1). Sequences were 100% identical to D. septosporum isolates in GenBank (e.g., AY808291). In addition, up to 12 randomly selected acervuli were excised directly from the needles of each of the three trees and DNA was extracted using PrepMan solution (Applied Biosystems). The mating types of these samples were determined using species-specific mating type primers for D. pini and D. septosporum (2). All acervuli were confirmed to be those of D. septosporum. Both mating types were found on needles from P. brutia and P. nigra. However, the teleomorph of D. septosporum was not detected on infected needles. References: (1) I. Barnes et al. For. Pathol. 41:361, 2011. (2) M. Groenewald et al. Phytopathology 97:825, 2007. (3) D. S. Kailidis and S. Markalas. Dasika Chronika 24:257, 1981 (in Greek).
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The genus Elsinoe includes many aggressive plant pathogens that infect various economically important agricultural, horticultural and forestry plants. Significant diseases include citrus scab caused by E. fawcettii and E. australis, grapevine spot anthracnose by E. ampelina, and the emerging Eucalyptus scab and shoot malformation disease caused by the recently described E. necatrix. Despite their importance as plant pathogens, little is known regarding the biology of many Elsinoe spp. To gain insights into the reproductive biology of these fungi, we characterized the mating-type loci of seven species using whole genome sequence data. Results showed that the MAT1 locus organization and its flanking genes is relatively conserved in most cases. All seven species manifested a typical heterothallic mating system characterized by having either the MAT1-1 or MAT1-2 idiomorph present in an isolate. These idiomorphs were defined by the MAT1-1-1 or the MAT1-2-1 gene, respectively. A unique MAT1-1 idiomorph containing a truncated MAT1-2-1 gene, and a MAT1-1-1 gene, was identified in E. necatrix and E. fawcettii genomes. Additionally, two idiomorph-specific proteins were found in the MAT1-1 and MAT1-2 idiomorphs of E. australis. Universal mating-type markers confirmed heterothallism across 21 Elsinoe spp., are poised to advance future studies regarding the biology of these fungi.
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Ascomicetos , Genes Fúngicos Tipo Acasalamento , Ascomicetos/genética , Reprodução/genéticaRESUMO
The NICE and PRECOG guidelines are based on systematic reviews of risk factors for pre-eclampsia to identify mothers at risk before 20 weeks' gestation. Cases (64) and controls (112) were classified retrospectively as screen positive or negative as recommended by the two guidelines The NICE guideline had a higher sensitivity rate of 77% (95% CI 65-87%) vs 59% (95% CI 46-71%) but a lower specificity of 54% (95% CI 44-64%) vs 81% (95% CI 73-88%) with the PRECOG guideline. Based on an incidence of pre-eclampsia of 4% the positive predictive values of PRECOG and NICE guidelines were estimated at only 11% and 7%, respectively. The most discriminatory risk factor was history of pre-eclampsia in a previous pregnancy. Neither guideline has a reasonable performance and cannot be recommended for use in clinical practice. Resources should rather be focussed on development of new strategies to identify women at risk of pre-eclampsia.
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Guias de Prática Clínica como Assunto , Pré-Eclâmpsia/diagnóstico , Diagnóstico Pré-Natal , Adulto , Feminino , Humanos , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Gravidez , Sensibilidade e EspecificidadeRESUMO
The giant deer, or 'Irish elk', has featured extensively in debates on adaptation, sexual selection, and extinction. Its huge antlers--the largest of any deer species, living or extinct--formed a focus of much past work. Yet the phylogenetic position of the giant deer has remained an enigma. On the basis of its flattened antlers, the species was previously regarded as closely related to the living fallow deer. Recent morphological studies, however, have challenged that view and placed the giant deer closer to the living red deer or wapiti. Here we present a new phylogenetic analysis encompassing morphological and DNA sequence evidence, and find that both sets of data independently support a sister-group relationship of giant and fallow deer. Our results include the successful extraction and sequencing of DNA from this extinct species, and highlight the value of a joint molecular and morphological approach.
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Cervos/classificação , Cervos/genética , Fósseis , Filogenia , Animais , Chifres de Veado/anatomia & histologia , Teorema de Bayes , Osso e Ossos/anatomia & histologia , DNA Mitocondrial/análise , DNA Mitocondrial/genética , DNA Mitocondrial/isolamento & purificação , Cervos/anatomia & histologia , Laringe/anatomia & histologia , Masculino , Dados de Sequência Molecular , Polimorfismo Genético/genética , Fatores de TempoRESUMO
BACKGROUND: Incidence rates for many cancers are lower in India than in Britain and it is therefore of interest to compare rates in British Indians to British whites, as well as to rates in India. We present estimates for Leicester, which has the largest population of Indian origin in Britain, and also has virtually complete, self-assigned, ethnicity data. METHODS: We obtained data on all cancer registrations from 2001 to 2006 for Leicester with ethnicity data obtained by linkage to the Hospital Episode Statistics database. Age-standardised incidence rates were calculated for British Indians and British whites as well as incidence rate ratios, adjusted for age and income. RESULTS: Incidence rate ratios for British Indians compared with British whites were significantly less than 1.0 for all cancers combined (0.65) and for cancer of the breast (0.72), prostate (0.76), colon (0.46), lung (0.30), kidney (0.36), stomach (0.54), bladder (0.48) and oesophagus (0.64), but higher than 1.0 for liver cancer (1.95). CONCLUSION: These results are likely to be the most accurate estimate of cancer incidence in British Indians to date and confirm that cancer incidence in British Indians is lower than in British whites in Leicester, particularly for cancer of the breast, prostate, colon and lung (and other smoking-related cancers), but much higher than in India.
Assuntos
Neoplasias/etnologia , Adulto , Idoso , Feminino , Humanos , Incidência , Índia , Masculino , Pessoa de Meia-Idade , Neoplasias/epidemiologia , Sistema de Registros , Fatores de Tempo , Reino Unido/epidemiologia , População BrancaRESUMO
Ceratocystis accommodates many important pathogens of agricultural crops and woody plants. Ceratocystis fimbriata, the type species of the genus is based on a type that is unsuitable for a precise application and interpretation of the species. This is because no culture or DNA data exist for the type specimen. The aim of this study was to select a reference specimen that can serve to stabilize the name of this important fungus. We selected a strain, CBS 114723, isolated from sweet potato in North Carolina, USA, in 1998 for this purpose. The strain was selected based on the availability of a living culture in a public depository. A draft genome sequence is also available for this strain. Its morphological characteristics were studied and compared with the existing and unsuitable type specimen as well as with the original descriptions of C. fimbriata. The selected strain fits the existing concept of the species fully and we have consequently designated it as an epitype to serve as a reference specimen for C. fimbriata.