RESUMO
Fine-needle aspiration cytology is the standard technique to diagnose thyroid pathologies. However, this method has a high percentage of inconclusive and false-negative results for benign and malignant lesions. Hence, it is important to search for a new method to assist medical evaluation during these surgical procedures. The use of time-resolved fluorescence techniques to detect biochemical composition and tissue structure alterations could help to develop a portable, minimally invasive, and non-destructive method to assist medical evaluation. In this study, we investigated 17 human thyroid samples by absorbance, fluorescence, excitation, and time-resolved fluorescence measurements. This initial investigation has demonstrated that thyroid fluorescence originates from many endogenous fluorophores and culminates in several bands. The fluorescence lifetimes of benign and malignant lesions were significantly different, as attested by analysis of variance using Tukey test with individual confidence level of 98.06%. Our results suggest that fluorescence lifetimes of benign and malignant lesions can potentially assist diagnosis. After further investigations, fluorescence methods could become a tool for the surgeon to identify differences between normal and pathological thyroid tissues.
Assuntos
Carcinoma/patologia , Bócio/patologia , Fenômenos Ópticos , Glândula Tireoide/citologia , Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/patologia , Carcinoma Papilar , Estudos de Casos e Controles , Humanos , Projetos Piloto , Espectrometria de Fluorescência , Câncer Papilífero da TireoideRESUMO
A case of neonatal hemoperitoneum secondary to umbilical artery catheterization is presented. The only sign of arterial laceration was a rapid accumulation of intraperitoneal fluid following placement of the catheter.