Should early weightbearing be allowed after intramedullary fixation of trochanteric femur fractures? A finite element study.

BACKGROUND: This study aims to investigate the effects of early weightbearing after intramedullary fixation of trochanteric fractures. METHODS: Femurs with different types of trochanteric fractures were modeled according to AO/OTA classification. Fractures were ideally reduced with one mm gap between fragments and fixed with intramedullary nails. Forces were applied simulating single- (Body weight: 60 kg, joint reaction force: 1999.2 N, abductor muscle force:1558.8 N) and double-leg standing positions (Joint reaction force: 196 N). In another model, a 500 Nm rotational force was applied as a simulation of a fall. RESULTS: A higher level of stress was determined at the calcar femorale, the fracture site, the holes for the lag screws, and the hole for the proximal locking screw on the nail, the threadless parts of the lag screws, and the mid-portion of the nail. During the single-leg stance, up to 3 mm displacement was observed with the reverse oblique type of fractures. In the simulation of the fall, 1.5 mm displacement occurred at the fracture site. No displacement was measured at stabile and type 31A2 fracture models. In addition, higher levels of stress were measured at the body of the nail (up to 133 MPa), proximal screws (up to 133 MPa) and at the bone distal to the nail (up to 84.3 MPa), but all values were under the limit of the yield stress of the bone and the titanium. CONCLUSION: Full weightbearing after intramedullary fixation of trochanteric femur fractures may be allowed except in obese patients and patients with 31A3 type fractures according to the AO/OTA classification. The use of support is recommended in order to prevent complications. Implant removal can be discussed with patients after fracture union in order to prevent possible periprosthetic fractures.

Platelet-rich plasma for enhancing surgical rotator cuff repair: evaluation and comparison of two application methods in a rat model.

PURPOSE: Platelet-rich plasma (PRP) is a natural concentrate of autologous growth factors now being widely tested in different fields of medicine for its potential in enhancing the regeneration of tissue with low healing potential. However, studies of PRP in enhancing rotator cuff repair have been contradictory, perhaps because of how PRP is administered. The purpose of this study is to evaluate the effect of PRP and compare two different application methods of PRP on rotator cuff healing. METHODS: The supraspinatus tendons of 48 mature, male Wistar-Albino rats were detached from their insertion on the humerus. The animals were divided into four groups: (1) no repair, (2) primary repair, (3) repair plus PRP injections into the tendon-bone interface, and (4) repair plus PRP absorbed from a sponge carrier to the tendon-bone interface. The tendons were evaluated biomechanically and histologically at week 8. RESULTS: Cuffs repaired with PRP had significantly greater mean (SD) load-to-failure rates [11.1 (6.5) and 11.6 (3.9) N; P < 0.05] and stiffness [3.5 (2.3) and 1.6 (0.75) N; P < 0.05] than did cuffs repaired without PRP. The groups receiving PRP did not differ significantly on these variables. Histological evaluation showed no significant differences among the four groups. CONCLUSIONS: The application of PRP, independent of the application method, significantly improved biomechanical properties at the rotator cuff tendon-bone interface. The type of application, injection or absorption from a sponge did not influence the effect of PRP on rotator cuff healing.

Horizontal suture placement influences meniscal repair fixation strength.

PURPOSE: This in vitro biomechanical study investigated the influence of horizontal suture placement distance from the medial meniscal lesion repair site on fixation characteristics during submaximal cyclic and load to failure test conditions. METHODS: Eighteen cadaveric (20-45 years of age) medial menisci with intact joint capsules were harvested within 24-48 h after death and divided into two groups of 9 specimens each for biomechanical testing. A 2.0-cm-long antero-posterior vertical longitudinal lesion was created with a #15 scalpel 2.0-3.0 mm from the outer edge of each meniscus. Menisci were repaired using #2-0 suture material with two horizontal suture loops placed either 1.0 mm (Group 1) or 3.0 mm (Group 2) from the lesion site. Following repair, the lesion was extended completely through the meniscal horns so that no tissue secured the repair, only the two horizontal suture loops representing a "worst-case" testing scenario. Following repair, specimens were placed in a servo hydraulic device using a pair of 1.2-mm-diameter steel wire loops and underwent submaximal cyclic loading between 5 and 50 N (1 Hz) for 500 cycles prior to load to failure testing (5 mm/min crosshead speed, 20 Hz data collection). An alpha level of P < 0.05 was selected to indicate statistical significance. RESULTS: Five of nine (55.6 %) Group 1 specimens did not complete submaximal cyclic testing. All Group 2 specimens completed submaximal cyclic testing (Fisher's exact test P = 0.029). Statistically significant mean group differences were not observed for displacement during submaximal cyclic loading (Group 1 = 5.0 ± 1.5 mm and Group 2 = 5.7 ± 1.6 mm) or for construct stiffness during load to failure testing (Group 1 = 50.1 ± 6.3 N/mm and Group 2 = 52.6 ± 11.9 N/mm). Group 2 displayed greater mean load at failure (112.1 ± 40.8 N vs. 72.7 ± 11.2 N, P = 0.02) and mean displacement at failure (11.1 ± 2.2 mm vs. 7.6 ± 1.4 mm, P = 0.03) than Group 1. CONCLUSIONS: Horizontal sutures placed slightly farther away from the meniscus lesion displayed superior repair fixation than sutures placed closer to the lesion. The superior biomechanical meniscal repair fixation provided by capturing greater tissue volume may enable safe earlier participation in functional exercise activities. Studies are needed to verify these findings in vivo.

Tendon-grasping strength of various suture configurations for rotator cuff repair.

PURPOSE: The purpose of the present study is to evaluate the mechanical performance and initial strength of the arthroscopic Mason-Allen, double mattress, inclined Mason-Allen, and lasso loop stitch configurations. METHODS: Using 36 sheep infraspinatus tendons, tendon widths and thicknesses were measured with a digital caliper to confirm standardization of the tendons. Four different stitch configurations (Mason-Allen, inclined Mason-Allen, double mattress, and lasso loop) were biomechanically tested with cyclic loading followed by load to failure testing. The cyclic elongation, peak-to-peak displacement, ultimate tensile strength, stiffness, and mode of failure were recorded. RESULTS: Mean tendon widths and thicknesses were statistically the same. The lasso loop (0.7 ± 0.1 mm) demonstrated a mean cyclic elongation greater than the Mason-Allen (0.5 ± 0.3 mm) and double mattress (0.5 ± 0.3 mm) groups (P = 0.011; P = 0.013). No differences were found in ultimate failure load, stiffness, and peak-to-peak displacement for the Mason-Allen (mean 99 ± 42 N, 39 ± 9 N/mm, 0.6 ± 0,1 mm), inclined Mason-Allen (113 ± 52 N, 44 ± 14 N/mm, 0.5 ± 0.1 mm), double mattress stitch (119 ± 68 N, 45 ± 10 N/mm, 0.5 ± 0.1 mm), or lasso loop (100 ± 38 N, 42 ± 7 N/mm, 0.5 ± 0.1 mm) groups (n.s.). Each specimen failed at the suture-tendon interface. Three specimens (two Mason-Allen and one inclined Mason-Allen) failed during cyclic testing. CONCLUSION: Conventional Mason-Allen configuration can be applied with double-loaded suture anchor safely. Recent modifications of the configurations offer no biomechanical advantage.

The impact of frenulum height on strains in maxillary denture bases.

PURPOSE: The midline fracture of maxillary complete dentures is a frequently encountered complication. The purpose of this study was to assess the effect of frenulum height on midline strains of maxillary complete dentures. MATERIALS AND METHODS: A removable maxillary complete denture was fabricated and duplicated seven times. Four different labial frenulum heights were tested for stresses occurring on the palatal cameo surface. The strains were measured with strain gauges placed on 5 different locations and the stresses were calculated. To mimic occlusal forces bilaterally 100 N of load was applied from the premolar and molar region. RESULTS: A statistically significant association between the height of the labial frenulum and the calculated stresses and strains was shown (P<.05) predominantly on the midline and especially on the incisive papilla. The results showed that stress on the anterior midline of the maxillary complete denture increases with a higher labial frenulum. CONCLUSION: Within the limitations of this in vitro study, it can be concluded that the stress on the anterior midline of the maxillary complete denture increases with a higher labial frenulum. Surgical or mechanical precautions should be taken to prevent short-term failure of maxillary complete dentures due to stress concentration and low cycle fatigue tendency at the labial frenulum region.

Cisplatin loaded PMMA: mechanical properties, surface analysis and effects on Saos-2 cell culture.

OBJECTIVE: Despite wide resection and systemic chemotherapy, bone tumors may present with local recurrences, metastases and pathological fractures. Application of bone cement containing antineoplastic drug to fill the defect after resection of metastatic lesions and to support implants has been suggested to prevent local tumor growth and implant failures. In this study, we aimed to demonstrate the effects of the addition of cisplatin which is a widely used antineoplastic drug for osteosarcoma, on the mechanical properties of bone cement, and to evaluate the cytotoxic effects of eluted cisplatin on Saos-2 cell culture. METHODS: Two cement samples were prepared by mixing 100 mg and 300 mg of cisplatin powder with 40 g cement powder. The bone cement of the control group did not contain cisplatin. Mechanical analyses included 4-point bending, compression and shear testing. For cytotoxicity analysis, samples were incubated in Dulbecco's Modified Eagle's medium for 15 days. Mediums were applied to Saos-2 cell culture and cell viability was measured. Surface analyses were performed by scanning electron microscope (SEM). RESULTS: The addition of cisplatin did not alter the mechanical properties of bone cement. It was observed that the eluted cisplatin had cytotoxic effects on Saos-2 cells. SEM analyses demonstrated cisplatin granules on the surface of cement samples. CONCLUSION: Cisplatin maintains its cytotoxic property when released from bone cement without compromising the mechanical stability. Application of cisplatin loaded bone cement may help local control of tumor growth. We believe that our study will shed light on to these new practices for the treatment of bone cancers and will encourage future studies.

Effect of platelet-rich plasma on tendon-to-bone healing after rotator cuff repair in rats: an in vivo experimental study.

OBJECTIVE: The purpose of this experimental study was to analyze the effects of local autologous platelet-rich plasma (PRP) injection on tendon-to-bone healing in a rotator cuff repair model in rats. METHODS: Rotator cuff injury was created in 68 left shoulders of rats. PRP was obtained from the blood of an additional 15 rats. The 68 rats were divided into 4 groups with 17 rats in each group; PRP group (Week 2), control group (Week 2), PRP group (Week 4), and control group (Week 4). Platelet-rich plasma or saline was injected to the repair area intraoperatively. Rats were sacrificed 2 and 4 weeks after the surgery. Histological analysis using a semiquantitative scoring was performed on 7 rats per group. Tendon integrity and increases in vascularity and inflammatory cells and the degree of new bone formation were evaluated and compared between the groups. The remaining tendons (n=10) were mechanically tested. RESULTS: Degree of inflammation and vascularity were less in the study group at both time intervals (p<0.05). Tendon continuity was better in the study group at 2 weeks (p<0.05). Obvious new bone formation was detected in the control group at 4 weeks (p<0.05). Biomechanically, platelet-rich plasma-treated specimens were stronger at 2 weeks (p<0.05). CONCLUSION: Local autologous PRP injection may have beneficial effects on initial rotator cuff tendon-to-bone healing and enhance initial tendon-to-bone healing remodeling. This may represent a clinically important improvement in rotator cuff repair.