RESUMO
Hedgehog (Hh) proteins comprise a family of secreted signaling molecules essential for patterning a variety of structures in animal embryogenesis. During biosynthesis, Hh undergoes an autocleavage reaction, mediated by its carboxyl-terminal domain, that produces a lipid-modified amino-terminal fragment responsible for all known Hh signaling activity. Here it is reported that cholesterol is the lipophilic moiety covalently attached to the amino-terminal signaling domain during autoprocessing and that the carboxyl-terminal domain acts as an intramolecular cholesterol transferase. This use of cholesterol to modify embryonic signaling proteins may account for some of the effects of perturbed cholesterol biosynthesis on animal development.
Assuntos
Colesterol/metabolismo , Proteínas de Drosophila , Indução Embrionária , Proteínas/metabolismo , Transativadores , Animais , Linhagem Celular , Células Cultivadas , Ditiotreitol/farmacologia , Drosophila , Desenvolvimento Embrionário e Fetal , Proteínas Hedgehog , Humanos , Processamento de Proteína Pós-Traducional , Proteínas/genética , Transdução de SinaisRESUMO
Veratrum alkaloids and distal inhibitors of cholesterol biosynthesis have been studied for more than 30 years as potent teratogens capable of inducing cyclopia and other birth defects. Here, it is shown that these compounds specifically block the Sonic hedgehog (Shh) signaling pathway. These teratogens did not prevent the sterol modification of Shh during autoprocessing but rather inhibited the response of target tissues to Shh, possibly acting through the sterol sensing domain within the Patched protein regulator of Shh response.
Assuntos
Sistema Nervoso Central/embriologia , Colesterol/metabolismo , Proteínas/metabolismo , Teratogênicos/farmacologia , Transativadores , Fatores de Transcrição , Alcaloides de Veratrum/farmacologia , Anormalidades Induzidas por Medicamentos/etiologia , Animais , Membrana Celular/metabolismo , Sistema Nervoso Central/efeitos dos fármacos , Sistema Nervoso Central/metabolismo , Embrião de Galinha , Colesterol/biossíntese , Técnicas de Cultura , Proteínas de Ligação a DNA/biossíntese , Retículo Endoplasmático/metabolismo , Proteínas Hedgehog , Fator 3-beta Nuclear de Hepatócito , Holoprosencefalia/induzido quimicamente , Proteínas de Homeodomínio/biossíntese , Proteínas com Homeodomínio LIM , Proteínas de Membrana/metabolismo , Proteínas Musculares/biossíntese , Proteínas do Tecido Nervoso/biossíntese , Proteínas Nucleares/biossíntese , Fator de Transcrição PAX7 , Receptores Patched , Receptores de Superfície Celular , Transdução de Sinais/efeitos dos fármacos , Tomatina/análogos & derivados , Tomatina/farmacologia , Triparanol/farmacologia , Dicloridrato de trans-1,4-Bis(2-clorobenzaminometil)ciclo-hexano/farmacologiaRESUMO
Extracellular signaling proteins encoded by the hedgehog (hh) multigene family are responsible for the patterning of a variety of embryonic structures in vertebrates and invertebrates. The Drosophila hh gene has now been shown to generate two predominant protein species that are derived by an internal autoproteolytic cleavage of a larger precursor. Mutations that reduced the efficiency of autoproteolysis in vitro diminished precursor cleavage in vivo and also impaired the signaling and patterning activities of the HH protein. The two HH protein species exhibited distinctive biochemical properties and tissue distribution, and these differences suggest a mechanism that could account for the long- and short-range signaling activities of HH in vivo.
Assuntos
Proteínas de Drosophila , Drosophila/metabolismo , Embrião não Mamífero/metabolismo , Processamento de Proteína Pós-Traducional , Proteínas/metabolismo , Transdução de Sinais , Sequência de Aminoácidos , Animais , Linhagem Celular , Drosophila/embriologia , Drosophila/genética , Indução Embrionária , Regulação da Expressão Gênica no Desenvolvimento , Genes de Insetos , Proteínas Hedgehog , Modelos Biológicos , Dados de Sequência Molecular , Mutação , Precursores de Proteínas/química , Precursores de Proteínas/genética , Precursores de Proteínas/metabolismo , Proteínas/química , Proteínas/genética , Serina Endopeptidases/químicaRESUMO
One of the most dominant influences in the patterning of multicellular embryos is exerted by the Hedgehog (Hh) family of secreted signaling proteins. Here, we identify a segment polarity gene in Drosophila melanogaster, skinny hedgehog (ski), and show that its product is required in Hh-expressing cells for production of appropriate signaling activity in embryos and in the imaginal precursors of adult tissues. The ski gene encodes an apparent acyltransferase, and we provide genetic and biochemical evidence that Hh proteins from ski mutant cells retain carboxyl-terminal cholesterol modification but lack amino-terminal palmitate modification. Our results suggest that ski encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of Hh, and further demonstrate that this lipid modification is required for the embryonic and larval patterning activities of the Hh signal.
Assuntos
Aciltransferases/genética , Aciltransferases/metabolismo , Proteínas de Drosophila , Drosophila melanogaster/genética , Proteínas de Insetos/metabolismo , Ácido Palmítico/metabolismo , Transdução de Sinais , Acilação , Aciltransferases/química , Sequência de Aminoácidos , Substituição de Aminoácidos , Animais , Padronização Corporal , Colesterol/metabolismo , Drosophila melanogaster/embriologia , Drosophila melanogaster/crescimento & desenvolvimento , Drosophila melanogaster/metabolismo , Expressão Gênica , Genes de Insetos , Proteínas Hedgehog , Proteínas de Insetos/química , Proteínas de Insetos/genética , Dados de Sequência Molecular , Mutação , Estrutura Terciária de Proteína , TransgenesRESUMO
A Sonic hedgehog (Shh) response element was identified in the chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) promoter that binds to a factor distinct from Gli, a gene known to mediate Shh signaling. Although this binding activity is specifically stimulated by Shh-N (amino-terminal signaling domain), it can also be unmasked with protein phosphatase treatment in the mouse cell line P19, and induction by Shh-N can be blocked by phosphatase inhibitors. Thus, Shh-N signaling may result in dephosphorylation of a target factor that is required for activation of COUP-TFII-, Islet1-, and Gli response element-dependent gene expression. This finding identifies another step in the Shh-N signaling pathway.
Assuntos
Proteínas de Ligação a DNA/genética , Regulação da Expressão Gênica , Fosfoproteínas Fosfatases/metabolismo , Proteínas/genética , Proteínas/metabolismo , Receptores de Esteroides , Transativadores , Fatores de Transcrição/genética , Animais , Fator II de Transcrição COUP , Fatores de Transcrição COUP , Linhagem Celular , DNA/metabolismo , Proteínas de Ligação a DNA/metabolismo , Inibidores Enzimáticos/farmacologia , Proteínas Hedgehog , Toxinas Marinhas , Camundongos , Ácido Okadáico/farmacologia , Oxazóis/farmacologia , Fosfoproteínas Fosfatases/antagonistas & inibidores , Fosforilação , Regiões Promotoras Genéticas , Transdução de Sinais , Fatores de Transcrição/metabolismoRESUMO
The bithorax complex in Drosophila melanogaster is a cluster of homeotic genes that specify developmental pathways for many of the body segments of the fly. The DNA of the bithorax complex has been isolated, and a region of 195,000 base pairs that covers the left half of the complex is described here. The lesions associated with many of the bithorax complex mutants have been identified, and most are due to DNA rearrangements. Most of the spontaneous mutants have insertions of a particular mobile element named "gypsy." This element affects the functions of sequences removed from the site of insertion. Mutant lesions for a given phenotypic class are distributed over large DNA distances of up to 73,000 base pairs.
RESUMO
Midbrain dopaminergic neurons, whose loss in adults results in Parkinson's disease, can be specified during embryonic development by a contact-dependent signal from floor plate cells. Here we show that the amino-terminal product of Sonic hedgehog autoproteolysis (SHH-N), an inductive signal expressed by floor plate cells, can induce dopaminergic neurons in vitro. We show further that manipulations to increase the activity of cyclic AMP-dependent protein kinase A, which is known to antagonize hedgehog signaling, can block dopaminergic neuron induction by floor plate cells. Our results and those of other studies indicate that SHH-N can function in a dose-dependent manner to induce different cell types within the neural tube. Our results also provide the basis for a potential cell transplantation therapy for Parkinson's disease.
Assuntos
Dopamina/fisiologia , Indução Embrionária/fisiologia , Mesencéfalo/citologia , Neurônios/metabolismo , Proteínas/metabolismo , Transativadores , Animais , Células Cultivadas/metabolismo , Meios de Cultura , Proteínas Quinases Dependentes de AMP Cíclico/agonistas , Proteínas Hedgehog , Hibridização In Situ , Mesencéfalo/metabolismo , Camundongos , Fragmentos de Peptídeos/metabolismo , Proteínas/isolamento & purificação , Proteínas/farmacologia , Ratos , Proteínas Recombinantes/metabolismoRESUMO
The disease holoprosencephaly is the basis of the most common structural anomaly of the developing forebrain in humans. Numerous teratogens when administered during early gastrulation, have been associated with this condition. Recent studies have characterized molecules expressed in the prechordal plate which are critical for normal brain formation. Perturbation of signaling pathways involving these molecules have been shown to cause holoprosencephaly in humans and other organisms.
Assuntos
Holoprosencefalia/embriologia , Holoprosencefalia/genética , Prosencéfalo/embriologia , Animais , Desenvolvimento Embrionário e Fetal , Holoprosencefalia/induzido quimicamente , Humanos , Morfogênese , Transdução de Sinais , TeratogênicosRESUMO
BACKGROUND: Sonic hedgehog (Shh), a vertebrate homolog of the Drosophila segment polarity gene hedgehog (hh), has been implicated in patterning of the developing chick limb. Such a role is suggested by the restricted expression of Shh along the posterior limb bud margin, and by the observation that heterologous cells expressing Shh have limb-polarizing activity resembling that of cells from the polarizing region of the posterior limb bud margin. It has not been demonstrated, however, that the Sonic hedgehog protein (SHH) alone is sufficient for limb patterning. SHH has been shown to undergo autoproteolytic cleavage in vitro, yielding two smaller products. It is of interest, therefore, to determine whether processing of SHH occurs in the developing limb and how such processing influences the function of SHH. RESULTS: We demonstrate that SHH is proteolytically processed in developing chick limbs. Grafts of cells expressing SHH protein variants that correspond to individual cleavage products demonstrate that the ability to induce patterned gene expression and to impose morphological pattern upon the limb bud is limited to the amino-terminal product (SHH-N) of SHH proteolytic cleavage. We also demonstrate that bacterially synthesized and purified SHH-N, released from implanted beads, is sufficient for limb-patterning activity. Finally, we show that the endogenous amino-terminal cleavage product is tightly localized to the posterior margin of the limb bud. CONCLUSIONS: Our data show that, of the two cleavage products resulting from SHH autoproteolysis, SHH-N expressed in grafted heterologous cells or supplied in purified form is sufficient to impose pattern upon the developing limb. Moreover, the restricted localization of the endogenous amino-terminal SHH cleavage product to the posterior border of the chick limb bud makes it unlikely that its patterning activity results from it being distributed in a broad gradient across the antero-posterior axis. More consistent with the observed localization is a model in which the amino-terminal SHH cleavage product exerts its patterning effects by local induction in or near the polarizing region, initiating a cascade of gene expression that ultimately extends across the developing limb.
Assuntos
Indução Embrionária/fisiologia , Proteínas/fisiologia , Transativadores , Animais , Linhagem Celular , Embrião de Galinha , Indução Embrionária/genética , Extremidades/embriologia , Proteínas Hedgehog , Processamento de Proteína Pós-Traducional , Proteínas/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismoRESUMO
BACKGROUND: The hedgehog (hh) family of secreted signaling proteins is responsible for developmental patterning in a variety of systems, including the neural tube, limbs and somites. Within the neural tube, at the level of the spinal cord, products of the vertebrate gene sonic hedgehog (shh) are proposed to function as a ventral patterning influence, with the capability of inducing floor plate and motor neurons. RESULTS: We report the isolation of tiggy-winkle hedgehog (twhh), a novel member of the zebrafish hh gene family. Both twhh and shh are expressed in the ventral midline of the embryonic zebrafish neural tube and brain, but twhh expression becomes limited to the neural tube, whereas shh is also expressed in the notochord. Both genes are expressed in the developing brain, in domains that include a discrete region in the floor of the diencephalon, located between the sites of the future optic stalks. Using pax-2 and pax-6 as markers of proximo-distal fate within the developing eye, we found that ectopic expression of either hh gene promoted proximal fates and suppressed distal fates. In contrast, proximal fates were lost in cyclops mutant embryos, which lack twhh- and shh-expressing forebrain cells. Both twhh and shh proteins undergo autoproteolytic processing in vivo; a fragment corresponding to the amino-terminal cleavage product was sufficient to carry out all signaling activities associated with twhh in eye and brain development. CONCLUSIONS: These findings suggest that secreted signals encoded by members of the hedgehog gene family, emanating from the ventral midline of the neural tube, not only play important roles in dorso-ventral patterning of the brain but also appear to constitute an early patterning activity along the proximo-distal axis of the developing eyes.
Assuntos
Encéfalo/embriologia , Proteínas de Drosophila , Olho/embriologia , Regulação da Expressão Gênica no Desenvolvimento , Proteínas/metabolismo , Transativadores , Sequência de Aminoácidos , Animais , Sequência de Bases , Encéfalo/metabolismo , Primers do DNA , Drosophila/genética , Indução Embrionária/genética , Olho/metabolismo , Proteínas Hedgehog , Mesoderma , Dados de Sequência Molecular , Notocorda/embriologia , Notocorda/metabolismo , Proteínas/genética , Homologia de Sequência de Aminoácidos , Peixe-Zebra/genética , Proteínas de Peixe-ZebraRESUMO
The pioneering work of E.B. Lewis drew attention early on to the developmental role of Ultrabithorax (Ubx), one of a group of homeotic genes that specify the distinguishing features of Drosophila segments. Recent molecular work sheds light on the genetic complexity discovered by Lewis and demonstrates that Ubx executes its developmental role by producing a family of closely related DNA-binding proteins that can function as transcription factors.
Assuntos
Drosophila/genética , Genes Homeobox , Animais , Mapeamento Cromossômico , DNA/metabolismo , Proteínas de Ligação a DNA/genética , Regulação da Expressão Gênica , Fatores de Transcrição/fisiologiaRESUMO
Cooperativity in binding of regulatory proteins to multiple DNA sites can heighten the sensitivity and specificity of the transcriptional response. We report here the cooperative DNA-binding properties of a developmentally active regulatory protein encoded by the Drosophila homeotic gene Ultrabithorax (Ubx). We show that naturally occurring binding sites for the Ubx-encoded protein contain clusters of multiple individual binding site sequences. Such sites can form complexes containing a dozen or more Ubx-encoded protein molecules, with simultaneous cooperative interactions between adjacent and distant DNA sites. The distant mode of interaction involves a DNA looping mechanism; both modes appear to enhance transcriptional activation in a simple yeast assay system. We found that cooperative binding is dependent on sequences outside the homeodomain, and we have identified regions predicted to form coiled coils carboxy terminal to the homeodomains of the Ubx-encoded protein and several other homeotic proteins. On the basis of our findings, we propose a multisite integrative model of homeotic protein action in which functional regulatory elements can be built from a few high-affinity sites, from many lower-affinity sites, or from sites of some intermediate number and affinity. An important corollary of this model is that even small differences in binding of homeotic proteins to individual sites could be summed to yield large overall differences in binding to multiple sites. This model is consistent with reports that homeodomain protein targets contain multiple individual binding site sequences distributed throughout sizable DNA regions. Also consistent is a recent report that sequences carboxy terminal to the Ubx homeodomain can contribute to segmental specificity.
Assuntos
Proteínas de Ligação a DNA/metabolismo , DNA/metabolismo , Proteínas de Drosophila , Drosophila/metabolismo , Genes Homeobox , Proteínas de Homeodomínio , Fatores de Transcrição , Animais , Sequência de Bases , Sítios de Ligação , Clonagem Molecular , DNA/ultraestrutura , Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/ultraestrutura , Drosophila/genética , Cinética , Microscopia Eletrônica , Modelos Estruturais , Dados de Sequência Molecular , Oligodesoxirribonucleotídeos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/ultraestrutura , Saccharomyces cerevisiae , Transcrição GênicaRESUMO
The vertebrate forebrain is derived from the anterior neural plate, where anteroposterior, dorsoventral and local patterning mechanisms specify regional identify. The recent identification of genetic regulators of these processes has opened the way to elucidating how the major forebrain regions (i.e. cerebral cortex, basal ganglia, thalamus, and hypothalamus) are formed, and how molecular lesions in these processes cause human birth defects.
Assuntos
Prosencéfalo/embriologia , Animais , Feminino , Humanos , Gravidez , Prosencéfalo/fisiologia , Transdução de Sinais/fisiologiaRESUMO
The demonstration over 30 years ago that inhibitors of cholesterol biosynthesis disrupt animal development suggested an intriguing connection between fundamental cellular metabolic processes and the more global processes of embryonic tissue patterning. Adding a new dimension to this relationship is the more recent finding that the Hedgehog family of tissue patterning factors are covalently modified by cholesterol. Here we review the mechanism of the Hedgehog autoprocessing reaction that results in this modification, and compare this reaction to that undergone by other autoprocessing proteins. We also discuss the biological consequences of cholesterol modification, in particular the use of cholesterol as a molecular handle in the spatial deployment of the protein signal in developing tissues. Finally, the developmental consequences of chemical and genetic disruption of cholesterol homeostasis are summarized, along with the potential importance of cholesterol-rich lipid rafts in production of and response to the Hh signal.
Assuntos
Colesterol/química , Colesterol/metabolismo , Proteínas/química , ATPases Translocadoras de Prótons , Proteínas de Saccharomyces cerevisiae , Transativadores , Sequência de Aminoácidos , Animais , Proteínas de Transporte/química , Proteínas de Transporte/metabolismo , Drosophila , Indução Embrionária , Endodesoxirribonucleases/química , Proteínas Hedgehog , Glicoproteínas de Membrana/química , Glicoproteínas de Membrana/metabolismo , Modelos Químicos , Dados de Sequência Molecular , Nematoides , Proteínas/antagonistas & inibidores , Proteínas/metabolismo , Transdução de Sinais , Teratogênicos/farmacologia , Alcaloides de Veratrum/farmacologiaRESUMO
Many proteins involved in signal transduction and cell adhesion are characterized by the presence of an extracellular domain with repeated copies of a leucine-rich motif (LRR). Here we report the isolation and characterization of a novel gene, tlr (for Toll-like receptor), which encodes a protein containing multiple LRRs in its presumed extracellular domain, a single transmembrane segment and homology to the cytoplasmic domain of the interleukin 1 receptor in its presumed intracellular domain. The pattern of tlr expression at the extended germ band stage is characterized by 15 transverse stripes in the gnathal and trunk segments, with four patches of expression corresponding to head segments and an additional patch of expression in the presumptive hindgut. The segmentally repeated tlr stripes in the trunk overlap both the wingless and engrailed stripes and thus span the parasegment boundary. The tlr stripes require pair rule gene function for their establishment and later become dependent upon segment-polarity gene function for their maintenance. Segmental modulation of tlr expression later in the tracheal system is dependent upon the function of the homeotic genes of the bithorax complex. The tlr gene also is prominently expressed in the imaginal discs. In the eye disc, this expression occurs in two stripes at the anterior and posterior margins of the morphogenetic furrow; this expression is consistent with a genetic interaction between a tlr mutation and an eye-specific allele of hedgehog. All of these data combine to suggest a role for tlr in interactions between cells at critical boundaries during development.
Assuntos
Envelhecimento/metabolismo , Drosophila/genética , Olho/metabolismo , Sequência de Aminoácidos , Animais , Expressão Gênica , Código Genético , Leucina/análise , Dados de Sequência Molecular , Estrutura Terciária de Proteína , Sequências Repetitivas de Ácido Nucleico , Traqueia/metabolismoRESUMO
The Wnt and Hedgehog (Hh) signalling pathways have long been known to direct growth and patterning during embryonic development. Recent evidence also implicates these pathways in the postembryonic regulation of stem-cell number in epithelia such as those of the skin and intestine, which undergo constant renewal. A pathological role for the Wnt and Hh pathways has emerged from studies showing a high frequency of specific human cancers associated with mutations that constitutively activate the transcriptional response of these pathways. This article focuses on Hh and Wnt signal transduction and reviews evidence suggesting that tumorigenesis associated with pathway activation may result from mis-specification of cells towards stem-cell or stem cell-like fates.
Assuntos
Neoplasias/patologia , Neoplasias/fisiopatologia , Proteínas/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Transdução de Sinais , Transativadores , Proteínas de Peixe-Zebra , Animais , Padronização Corporal , Neoplasias do Colo/genética , Neoplasias do Colo/patologia , Neoplasias do Colo/fisiopatologia , Proteínas Hedgehog , Humanos , Mutação/genética , Neoplasias/genética , Proteínas/genética , Proteínas Proto-Oncogênicas/genética , Células-Tronco/metabolismo , Células-Tronco/patologia , Proteínas WntRESUMO
The Ubx and bxd transcription units comprise a single functional domain in the bithorax complex of Drosophila melanogaster. The segmental distributions and nuclear localization of proteins encoded by the Ubx unit have been determined by immunofluorescence staining with antibodies raised against a fusion protein containing Ubx coding sequences. Wild-type and mutant distributions are consistent with a model in which the protein-coding functions of the domain derive from the Ubx unit and are regulated by the bxd unit.
Assuntos
Drosophila melanogaster/crescimento & desenvolvimento , Regulação da Expressão Gênica , Fatores de Transcrição/análise , Animais , Anticorpos/análise , Sequência de Bases , Núcleo Celular/análise , Drosophila melanogaster/embriologia , Drosophila melanogaster/genética , Imunofluorescência , Larva , Modelos Genéticos , Morfogênese , Fatores de Transcrição/genética , Fatores de Transcrição/imunologia , Transcrição GênicaRESUMO
Cell-type specification in the Drosophila compound eye begins at the morphogenetic furrow. The furrow sweeps across the developing eye epithelium and is coincident with four classes of cellular events: coordinated changes in cell shape, changes in gene expression, synchronization of the cell cycle, and the specification of a regular array of ommatidial founder cells. The molecular mechanisms that induce these events in the developing eye have hitherto been unknown. We identify here a gene specifically required for furrow progression, hedgehog (hh). We show that hh expression posterior to the morphogenetic furrow is continuously required for its progression. We propose that forward diffusion of hh protein induces anterior cells to enter the furrow.
Assuntos
Proteínas de Drosophila , Drosophila melanogaster/embriologia , Células Fotorreceptoras de Invertebrados/embriologia , Proteínas/fisiologia , Animais , Olho/embriologia , Feminino , Expressão Gênica , Genes Dominantes , Genes de Insetos , Genes Supressores , Proteínas Hedgehog , Hormônios de Inseto/fisiologia , Proteínas de Membrana/fisiologia , Morfogênese , Mosaicismo , RNA Mensageiro/genética , Receptores de Superfície Celular , Retina/embriologiaRESUMO
Ci/Gli zinc finger proteins mediate the transcriptional effects of Hedgehog protein signals. In Drosophila, Ci action as transcriptional repressor or activator is contingent upon Hedgehog-regulated, PKA-dependent proteolytic processing. We demonstrate that PKA-dependent processing of vertebrate Gli3 in developing limb similarly generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The resulting anterior/posterior Gli3 repressor gradient can be perturbed by mutations of Gli3 in human genetic syndromes or by misregulation of Gli3 processing in the chicken mutant talpid2, producing a range of limb patterning malformations. The high relative abundance and potency of Gli3 repressor suggest specialization of Gli3 and its products for negative Hedgehog pathway regulation.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Botões de Extremidades/embriologia , Proteínas do Tecido Nervoso , Processamento de Proteína Pós-Traducional , Proteínas/metabolismo , Proteínas Repressoras , Transativadores , Fatores de Transcrição/metabolismo , Proteínas de Xenopus , Animais , Anticorpos/imunologia , Sequência de Bases , Padronização Corporal , Células COS , Embrião de Galinha , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , DNA Complementar , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/imunologia , Regulação da Expressão Gênica no Desenvolvimento , Proteínas Hedgehog , Humanos , Fatores de Transcrição Kruppel-Like , Camundongos , Dados de Sequência Molecular , Proteínas Nucleares , Fosforilação , Fatores de Transcrição/genética , Fatores de Transcrição/imunologia , Transcrição Gênica , Proteína Gli2 com Dedos de Zinco , Proteína Gli3 com Dedos de ZincoRESUMO
We have identified a novel Drosophila homeodomain gene, unplugged (unp), whose function is required for formation of the tracheal branches that penetrate the CNS. In unp mutant embryos the segmentally repeated ganglionic branches stall and fail to penetrate the CNS and the segment-specific cerebral branch and associated cerebral anastomosis fail to form. Expression of unp in the founder cells for the cerebral branch within the first tracheal metamere is repressed in posterior segments by Ubx and other bithorax complex genes. This pattern of expression and homeotic gene regulation is reproduced by an unusual 2.6 kb cis-regulatory sequence located downstream of the unp transcription unit. Since the unp protein is localized to the nucleus of tracheal precursor cells as they migrate and extend, unp protein appears to play a regulatory role in neural branching of the tracheae, and the segment-specific aspects of these neural branching patterns appear to be generated by homeotic regulation of unp expression.