Prevalence of plasmid-mediated quinolone resistance genes in extended-spectrum beta-lactamase producing Klebsiella pneumoniae isolates in northern Iran.

Plasmid-mediated quinolone resistance (PMQR) in extended-spectrum ß-lactamase (ESBL)-producing Klebsiella pneumoniae (K. pneumoniae) contributes to treatment failures, extended hospital stays, and increased mortality percentages. We aimed to determine the prevalence of PMQR genes in ESBL-producing K. pneumoniae isolates from clinical samples in Babol, North of Iran region. This is the first study in this region to investigate this specific association. A total of 95 K. pneumoniae isolates were obtained from hospitalized patients with various clinical infections during March 2022 to February 2023. Disk diffusion and Combination disk method were performed to identification of antimicrobial resistance profiles and ESBL-producing strains. The presence of ESBL and PMQR genes among K. pneumoniae isolates was assessed using polymerase chain reaction (PCR) method. Of the isolates, 68 (71.57 %) were considered as ESBL-producers. The bla TEM, bla SHV and bla CTX-M genes were detected in 74.73 %, 57.89 %, and 41.05 % of K. pneumoniae isolates, respectively. Among the PMQR encoding genes, the highest and lowest frequency was associated to qepA (67.3 %) and qnrA (4.2 %), respectively. The frequency of qnrA, qnrB, qnrS, acc (6')-Ib-cr, qepA, oqxA, and oqxB genes in 26 MDR-Kp isolates was 11.53 % (n; 3), 69.23 % (n; 18), 65.38 % (n; 17), 73.07 % (n; 19), 80.76 % (n; 21), 84.61 % (n; 22), and 76.92 % (n; 20), respectively. Our result revealed of the 68 ESBL gene-positive isolates, 60 (88.23 %) were positive for the PMQR gene. The co-occurrence of these genes within resistant isolates suggests potential linkage on mobile genetic elements such as plasmids. These findings highlight the significant burden of PMQR determinants in ESBL-producing K. pneumoniae and underscore the urgent need for effective control measures. Implementing robust antimicrobial stewardship programs and strengthening drug-resistance surveillance and control protocols are crucial to prevent the spread of resistant isolates.

Ag NPs decorated on the magnetic rod-like hydroxyapatite/MIL-101(Fe) nanocomposite as an efficient catalyst for the reduction of some nitroaromatic compounds and as an effective antimicrobial agent.

A rod-like magnetic nanocomposite was successfully synthesized in this work by loading Ag and Fe3O4 nanoparticles onto the surface of the hydroxyapatite/MIL-101(Fe) metal-organic framework. Various techniques were used to investigate the crystalline nature, size, morphology, and magnetic and structural properties of the HAP/MIL-101(Fe)/Ag/Fe3O4 nanocomposite, including X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, Raman spectroscopy, field-emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), BET surface area measurements, and zeta potential analysis. The results indicate that the nanocomposite sample is composed of Ag and Fe3O4 nanoparticles adhered to rod-like hydroxyapatite/MIL-101(Fe). The catalytic and antibacterial abilities of the as-prepared HAP/MIL-101(Fe)/Ag/Fe3O4 were studied. This nanocomposite was utilized as a heterogeneous catalyst for the catalytic reduction of toxic pollutants, including 4-nitrophenol (4-NP), 2-nitrophenol (2-NP), 2,4-dinitrophenol (2,4-NP), 4-nitroaniline (4-NA), and 2-nitroaniline (2-NA) by NaBH4 in water and at room temperature. These compounds were converted to their amine derivatives within 8-18 min with rate constant values equal to 0.2, 0.3, 0.33, and 0.47 min-1, respectively. This quaternary magnetic catalyst can be easily separated from the reaction medium using an external magnetic field and reused. The synthesized nanocomposite maintained its efficiency in reducing nitroaromatic compounds after 5 runs, showing the high stability of the catalyst. Besides, the antibacterial activity of the nanocomposite against Gram-negative and Gram-positive bacteria was evaluated using the disk diffusion method. The inhibition zone diameter of the nanocomposite against Staphylococcus aureus, Staphylococcus saprophyticus, and Escherichia coli was measured to be 17, 14, and 12 mm, respectively.

Adsorptive removal of tetracycline and ciprofloxacin drugs from water by using a magnetic rod-like hydroxyapatite and MIL-101(Fe) metal-organic framework nanocomposite.

A novel porous nanocomposite composed of hydroxyapatite nanorods (HAP), a MIL-101(Fe) metal-organic framework, and Fe3O4 nanoparticles was successfully fabricated in this work. The magnetic HAP/MIL-101(Fe)/Fe3O4 ternary nanocomposite was identified by various techniques, namely FT-IR spectroscopy, XRD, Raman spectroscopy, SEM, EDX, TEM, BET specific surface area, zeta potential, and VSM measurements. Tetracycline (TC) and ciprofloxacin (CIP) aqueous solutions were used to evaluate the adsorption performance of the resulting HAP/MIL-101(Fe)/Fe3O4 composite. The adsorption rate and capacity of HAP/MIL-101(Fe)/Fe3O4 were increased as compared with HAP, MIL-101(Fe), and HAP/MIL-101(Fe) samples due to the increased attraction. The influence of initial drug concentration, adsorbent dosage, temperature, and pH on the adsorption process was investigated. The results showed that the removal efficiencies of HAP/MIL-101(Fe)/Fe3O4 for TC and CIP were 95% and 93%, under the determined optimum conditions: pH of 7, drug concentration of 50 mg L-1, adsorbent dosage of 30 mg, and temperature of 25 °C. The maximum adsorption capacities of HAP/MIL-101(Fe)/Fe3O4 for TC and CIP were 120.48 mg g-1 and 112.35 mg g-1, respectively. Reusability of the prepared nanocomposite was easily achieved up to three times without significant change in its structure. As a result, the synthesized magnetic nanocomposite can be reused as a suitable absorbent for TC and CIP removal from aqueous solutions.

Trend in Prevalence of Hepatitis B Virus Infection Among Blood Donor Individuals: An Eleven-year of Experience in Lorestan, Iran.

BACKGROUND: Hepatitis B virus is one of the transfusion transmissible infections. Despite the availability of hepatitis B virus (HBV) vaccine and screening tests but still danger of virus transmission via blood transfusion is high in some regions. The objective of this study was to determine the trend of seroprevalence of hepatitis B in over an 11-year period (2005-2015). METHODS: In this study, 355,083 blood donors were estimated for hepatitis B surface antigen (HBs Ag) seropositivity during 2005-2015 who referred to blood infusion centers of Lorestan province. Third-generation ELISA method was used to detect HBs Ag. RESULTS: The prevalence of HBs Ag in blood donors was 0.29% (1017). It was decreased steadily from 2005 to 2015 (0.68% to 0.12%) but increased in 2008 year. The trend prevalence of HBs Ag seropositivity significantly decreased over the study period (P < 0.001). The decline in HBV infection rates was more prominent in regular and repeated donor's groups compared to people who donated blood for the first time (P < 0.001). CONCLUSIONS: The result of present study was indicated, Lorestan city in west of Iran can be classified as a low-income region because the low prevalence of HBs Ag in blood donors. Also the prevalence of HBs Ag in first-time donors was higher than other groups.

Antimicrobial activity of endophytic bacterial populations isolated from medical plants of Iran.

BACKGROUND AND OBJECTIVES: Endophytic actinobacteria colonize inside the plant tissues without causing damages to the host plant. Since these microorganisms colonize in the different parts of plants and can stop plant disease, they have been applied as biological agents for controlling human diseases. The aim of this study was molecular identification and measuring the antimicrobial activity of endophytic Actinomycetes isolated from medicinal plants of Iran. MATERIALS AND METHODS: The total of 23 medicinal plant samples were collected, sterilized, and crushed. Small pieces of the crushed samples were then cultured directly on three selective media. Grown colonies were identified by 16S rRNA gene sequencing method. Each isolate was cultured in TSB medium and then antimicrobial compound was extracted using ethyl acetate and tested against the target bacteria. RESULTS: Sixteen out of 23 bacterial isolates (69%) exhibited antimicrobial activity against the selected pathogenic bacteria, such as Bacillus cereus, Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, Citrobacter freundii, Proteus mirabilis, Shigella flexneri and Escherichia coli. CONCLUSION: Our Study showed a high phylogenetic diversity and the potent antibiotic activity of endophytic bacteria in medicinal plants of Iran.

Identification of clinical isolates of Acinetobacter baumannii from Iran and study of their heterogeneity.

BACKGROUND: Acinetobacter baumannii has become one of the most serious causative agents of nosocomial infections due to its significant ability to survive on hospital surfaces. It is mainly an emerging opportunistic pathogen infecting patients in intensive care units. This study was aimed to identify the clinical isolates of A. baumannii and to investigate their heterogeneity using polymerase chain reaction (PCR)-based typing methods. METHODS: A total of 197 nonduplicate isolates recovered from a wide range of clinical samples were subjected to conventional cultural and biochemical tests. For those isolates that were preliminary identified as A. baumannii, rpoB-based PCR with subsequent restriction fragment length polymorphism (RFLP) using two restriction enzymes (TagI and HaeIII) was performed to investigate the genetic diversity of the strains and their presumptive relationships with different clinical presentation of the disease caused by this pathogen. RESULTS: In total, 50 isolates (25.4%) were identified as A. baumannii using conventional phenotypic methods with subsequent confirmation by rpoB sequencing. RFLP analysis demonstrated five different restriction enzyme patterns, designated as A-E clusters. Most A. baumannii isolates were categorized under Cluster A (32%). We found no significant relationship between clinical presentation and the clustering of the isolates. CONCLUSION: This study showed that the rpoB region possesses high discriminatory power to identify the isolates to the species level. This marker showed high interspecies variability that might be useful for strain typing. The results also suggest the possibility of the existence of a predominant clone of A. baumannii among infected patients in Iran.