RESUMO
Multimodal nonlinear microscopy combining third-harmonic generation (THG) with two- and three-photon-excited fluorescence (2PEF and 3PEF) is shown to provide a powerful resource for high-fidelity imaging of nucleoli and nucleolar proteins. We demonstrate that, with a suitably tailored genetically encoded fluorescent stain, the 2PEF/3PEF readout from specific nucleolar proteins can be reliably detected against the extranucleolar 2PEF/3PEF signal, enabling high-contrast imaging of the key nucleolar ribosome biogenesis components, such as fibrillarin. THG is shown to provide a versatile readout for unstained nucleolus imaging in a vast class of biological systems as different as neurons in brain slices and cultured HeLa cells.
Assuntos
Microscopia , Fótons , Encéfalo , Células HeLa , Humanos , Imagem ÓpticaRESUMO
We present brain imaging experiments on rat cortical areas, demonstrating that, when combined with a suitable high-brightness, cell-specific genetically encoded fluorescent marker, three-photon-excited fluorescence (3PEF), enables subcellular-resolution, cell-specific 3D brain imaging that is fully compatible and readily integrable with other nonlinear-optical imaging modalities, including two-photon-fluorescence and harmonic-generation microscopy. With laser excitation provided by sub-100-fs, 1.25-µm laser pulses, cell-specific 3PEF from astrocytes and their processes detected in parallel with a three-photon-resonance-enhanced third harmonic from blood vessels is shown to enable a high-contrast 3D imaging of gliovascular interfaces.
Assuntos
Astrócitos/citologia , Vasos Sanguíneos/diagnóstico por imagem , Encéfalo/citologia , Encéfalo/diagnóstico por imagem , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Neuroglia/citologia , Animais , Imageamento Tridimensional , RatosRESUMO
We demonstrate stain-free, high-contrast, subcellular-resolution imaging of astroglial cells using epi-detected third-harmonic generation (THG). The astrocyte-imaging capability of THG is verified by colocalizing THG images with fluorescence images of astrocytes expressing a genetically encodable fluorescent reporter. We show that THG imaging with an optimized point-spread function can reliably detect significant subcellular features of astrocytes, including cell nuclei, as well as the soma shape and boundaries.
RESUMO
Optically detected electron spin resonance in fiber-coupled nitrogen-vacancy (NV) centers of diamond is used to demonstrate a fiber-optic quantum thermometry of individual thermogenetically activated neurons. Laser-induced temperature variations read out from single neurons with the NV-diamond fiber sensor are shown to strongly correlate with the fluorescence of calcium-ion sensors, serving as online indicators of the inward Ca2+ current across the cell membrane of neurons expressing transient receptor potential (TRP) cation channels. Local laser heating above the TRP-channel activation threshold is shown to reproducibly evoke robust action potentials, visualized by calcium-ion-sensor-aided fluorescence imaging and detected as prominent characteristic waveforms in the time-resolved response of fluorescence Ca2+ sensors.
Assuntos
Tecnologia de Fibra Óptica/métodos , Termometria/métodos , Diamante , Elétrons , Fluorescência , Lasers , Neurônios , Nitrogênio , Fibras Ópticas , TemperaturaRESUMO
Redox processes play a key role in cells of all.organisms. These processes imply directed flows of electrons via so-called redox pairs: substances that exist in both reduced and oxidized states simultaneously within the cell. Examples of redox pairs are NAD+/NADH, NADP+/NADPH, GSSG/2GSH. Until recently, studies of redox processes in the living cells were challenged by the lack of suitable methods. Genetically encoded fluorescent biosensors provide a new way to study biological processes including redox ones. Biosensors allow real-time detection of messengers, metabolites and enzymatic activities in living systems of different complexity from cultured cells to transgenic animals. In this review, we describe the main types of known redox biosensors with examples of their use.
Assuntos
Técnicas Biossensoriais , Peróxido de Hidrogênio/isolamento & purificação , Oxirredução , Superóxidos/isolamento & purificação , Animais , Dissulfeto de Glutationa/química , Proteínas de Fluorescência Verde/química , Peróxido de Hidrogênio/química , NAD/química , NADP/química , Espécies Reativas de Oxigênio/química , Superóxidos/químicaRESUMO
Most of the living cells maintain the continuous flow of electrons, which provides them by energy. Many of the compounds are presented in a cell at the same time in the oxidized and reduced states, forming the active redox couples. Some of the redox couples, such as NAD+/NADH, NADP+/NADPH, oxidized/reduced glutathione (GSSG/GSH), are universal, as they participate in adjusting of many cellular reactions. Ratios of the oxidized and reduced forms of these compounds are important cellular redox parameters. Modern research approaches allow setting the new functions of the main redox couples in the complex organization of cellular processes. The following information is about the main cellular redox couples and their participation in various biological processes.
Assuntos
Glutationa/metabolismo , NADP/metabolismo , NAD/metabolismo , Animais , Humanos , OxirreduçãoRESUMO
The localization of signaling molecules close to their targets is the central principle of cell signaling. The colocalization of multicomponent signaling complexes is realized through protein scaffolds that provide better specificity than undirected diffusion ofthe same components. ROS-generating complexes have been suggested to follow this principle by specific intracellular localization of ROS production and the limitation of ROS diffusion distances. However, the lack of adequate methods did not allow direct detection of local ROS production to confirm the model ofredox signaling compartmentalization. Nevertheless, evidences of local ROS production and restriction of diffusion were provided by kinetic modeling and data on the subcellular localization of NADPH-oxidase isoforms, their adapter proteins and local restriction of ROS diffusion. Here we shall discuss the properties of antioxidant system which prevents uncontrolled ROS diffusion from the sites of generation to the adjacent subcellular compartments; the current data of the specific localization NADPH-oxidases activity and its influence on intracellular processes; the recent evidences of the ROS diffusion restriction.
Assuntos
Compartimento Celular/genética , Peróxido de Hidrogênio/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/genética , Apoptose/genética , Movimento Celular/genética , Proliferação de Células , Retículo Endoplasmático/metabolismo , NADPH Oxidases/genética , NADPH Oxidases/metabolismo , Estresse Oxidativo/genética , Proteínas/metabolismoRESUMO
AIM: The aim of the study is to assess the efficiency of early invasive treatment versus conservative treatment for patients with non-ST acute coronary syndrome (ACS), and to study immediate results of percutaneous coronary intervention (PCI) in terms of the risk of adverse events. MATERIAL AND METHODS: The investigation includes 112 patients with non-ST ACS having risk of adverse outcome determined by the GRACE system. More than half of the patients (77/68.8%) underwent diagnostic coronary angiography within 72 hours of admission, and the decision was made on a subsequent treatment strategy (early invasive or conservative approach). In-hospital outcomes (cardiac deaths, nonfatal MI, reMI), cumulative frequency of these events have been studied according to the risk assessment scale GRACE and treatment strategy. RESULTS: Low risk was determined in 33.9% of patients, intermediate--in 32.1%, and high--in 33.9%. On the whole, frequency of myocardial revascularization was assessed as 56.3%. Fifty-one (45.5%) patients received early invasive treatment, 12 (10.7%)--coronary artery bypass grafting (CABG). A conservative approach was used in 49 cases (43.8%). An early invasive treatment was given to 20 patients (52.6%) from each of the low- and high-risk groups, 11 (30.6%)--from the intermediate risk group. In-hospital cardiac deaths were observed only in the high-risk group, and this number was higher in case of conservative treatment versus early invasive treatment: 3 (30%) versus 1 (50%). Non-fatal MIs in the form of re(MI) were also diagnosed only in the high-risk group following conservative treatment: 2 (20.0%). Nevertheless, no reliable difference in the frequency of each complication was found (p = 0.095; p = 0.1). Significant differences in the high-risk group were proven while comparing the impact of early invasive and conservative approaches on the cumulative frequency of in-hospital death and (re)MI cases (50.0% versus 5.0%; p = 0.009). No benefits of an early invasive approach were identified for low-risk and intermediate risk groups. CONCLUSION: Early invasive approach compared with a conservative approach in high risk patients with non-ST acute coronary syndrome can improve clinical outcomes with a decrease in the total frequency of in-hospital death, reMI.
Assuntos
Síndrome Coronariana Aguda/tratamento farmacológico , Síndrome Coronariana Aguda/cirurgia , Revascularização Miocárdica/métodos , Terapia Trombolítica/métodos , Síndrome Coronariana Aguda/diagnóstico , Síndrome Coronariana Aguda/mortalidade , Adulto , Idoso , Idoso de 80 Anos ou mais , Angiografia Coronária , Eletrocardiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Revascularização Miocárdica/estatística & dados numéricos , Medição de Risco , Índice de Gravidade de Doença , Análise de Sobrevida , Fatores de Tempo , Resultado do TratamentoRESUMO
Until recently, the production of reactive oxygen species by NADPH oxidase has been considered only in the context of the oxidative damage to pathogens inside the phagosome. However, homologues of phagocytic NADPH oxidase have been found in almost all cell types, where they produce hydrogen peroxide and thereby regulate the initial intracellular stages of MAP kinase cascades. In the present work, the activation of two MAP kinase cascades, p38 and Erk1/2, during phagocytosis has been studied. It was found that phagocytosis activates both cascades. The activation of Erkl/2 is dependent, and the activation of p38 is not dependent, on the activity of NADPH oxidase. Thus, it can be stated that the activation of MAP kinases in phagocytes during phagocytosis occurs by a mechanism similar to that operating in nonphagocytizing cells, indicating the universality of the function of NADPH oxidases in different cell types.
Assuntos
Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , NADPH Oxidases/fisiologia , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Animais , Linhagem Celular , Ativação Enzimática , Sistema de Sinalização das MAP Quinases , Camundongos , Fagócitos/metabolismo , FagocitoseRESUMO
Using HyPer, a ratiometric GFP-based biosensor, the dynamics of H2O2 in living cells has been studied. It was found that activation of the receptor of the epidermal growth factor (EGF) in epithelial cells leads to sustained generation of intracellular H2O2, which is blocked by apocynin, an inhibitor of the assembly of plasma membrane NADPH oxidase. Apocynin also blocked HeLa cell proliferation induced by EGF, indicating that NADPH oxidase should be involved in the process. However, apocynin failed to alter the kinetics of the EGF-stimulated ERK1/2 activation. It was concluded that NADPH oxidase and intracellular H2O2 are the important downstream targets of the EGF receptor, which mediate the proliferation response by mechanisms distinct from the activation of the classical ERK1/2 MAP-kinase pathway.
Assuntos
Fator de Crescimento Epidérmico/fisiologia , Proteína Quinase 1 Ativada por Mitógeno/fisiologia , Proteína Quinase 3 Ativada por Mitógeno/fisiologia , NADPH Oxidases/fisiologia , Acetofenonas/farmacologia , Técnicas Biossensoriais , Linhagem Celular Tumoral , Proliferação de Células , Receptores ErbB/agonistas , Receptores ErbB/fisiologia , Humanos , Peróxido de Hidrogênio/metabolismo , Peróxido de Hidrogênio/farmacologia , NADPH Oxidases/antagonistas & inibidoresRESUMO
AIM: To evaluate basic parameters of lipid metabolism in patients with arterial hypertension (AH) stage I and II and normotensive subjects living in the far North of the Russian Federation (Khanty-Mansiysk Autonomic Region, Nyagan). MATERIAL AND METHODS: Lipid spectrum was studied in 70 males and females with AH stage I and II, 280 normotensive males and females matched for sex, age, time of residence in the North. RESULTS: Sex-related differences by concentrations of blood lipids become less pronounced with progression of AH while a concentration of atherogenic lipid fractions in AH patients rises. High prevalence of dyslipidemia, primarily hypercholesterolemia, in hypertensive and normotensive subjects is a prognostic factor of coronary atherosclerosis. Paired correlations indirectly confirm the effect of climatic factors on blood lipids. CONCLUSION: It is necessary to correct lipid spectrum and risk factors in hypertensive and normotensive subjects as a prophylactic measure.
Assuntos
Hipercolesterolemia/sangue , Hipercolesterolemia/epidemiologia , Hipertensão/sangue , Hipertensão/epidemiologia , Lipídeos/sangue , Adulto , Artérias/fisiopatologia , Pressão Sanguínea , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Federação Russa/epidemiologia , Sibéria/epidemiologiaRESUMO
We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.
RESUMO
An analysis of amino acid sequences of small GTPases of the Ras-dva family allowed us to determine the C-terminal prenylation motif, which could be responsible for the membrane localization of these proteins. We demonstrated using in vivo EGFP tracing that the Ras-dva small GTPases from Xenopus laevis embryo cells and NIH-3T3 fibroblasts are localized on both plasma membranes and endomembranes (the endoplasmic reticulum, the Golgi apparatus, and vesicles). At the same time, the replacement of the Cys residue, the SH group of which must be theoretically farnesylated, in the C-terminal prenylation motif of the Ras-dva small GTPase by the Ser residue prevented the membrane localization of the protein. These results indicate that the C-terminal prenylation site is critical for the membrane localization of small Ras-dva GTPases.
Assuntos
Membrana Celular/enzimologia , Membranas Intracelulares/enzimologia , Proteínas Monoméricas de Ligação ao GTP/metabolismo , Prenilação de Proteína , Proteínas de Xenopus/metabolismo , Sequência de Aminoácidos , Animais , Proteínas de Fluorescência Verde/análise , Proteínas de Fluorescência Verde/genética , Camundongos , Dados de Sequência Molecular , Proteínas Monoméricas de Ligação ao GTP/análise , Proteínas Monoméricas de Ligação ao GTP/genética , Células NIH 3T3 , Proteínas de Xenopus/análise , Proteínas de Xenopus/genéticaRESUMO
A gas-tight and ductile solid/liquid δ-Bi2O3-0.2 wt% B2O3 electrolyte for molten oxide fuel cells (MOFCs) is developed. The MOFCs are a new class of intermediate temperature fuel cells. The composite, consisting of solid (δ-Bi2O3) and liquid (molten Bi2O3 + B2O3) oxygen ion-conducting phases, demonstrates the promising application as an MOFC electrolyte with the highest oxygen ionic conductivity.
Assuntos
Bismuto/química , Compostos de Boro/química , Fontes de Energia Elétrica , Condutividade Elétrica , Eletrólitos/química , Oxigênio/químicaRESUMO
We studied efficacy of repeated courses of prostatilen in suppositories with dimexide in prostatic adenoma patients with normal micturition. Rectal suppositories contain 30 mg prostatilen and 90 mg dimexide. The course consisted of 15 suppositories. The treatment reduced clinical symptoms of infravesical obstruction, residual urine volume in administration of prostatilen in 15-day courses each 3 months. This suggests possibility of suppository prostatilen use not only as an alternative for expensive drugs but also in combination with them in treatment of initial prostatic adenoma.
Assuntos
Peptídeos/uso terapêutico , Hiperplasia Prostática/tratamento farmacológico , Administração Retal , Idoso , Idoso de 80 Anos ou mais , Dimetil Sulfóxido/administração & dosagem , Dimetil Sulfóxido/uso terapêutico , Combinação de Medicamentos , Humanos , Masculino , Pessoa de Meia-Idade , Peptídeos/administração & dosagem , Supositórios , Resultado do TratamentoRESUMO
The authors review the experience with prostatilen treatment gained in the urological clinic of the St-Petersburg State Medical University and analyze treatment outcomes for 96 patients with benign prostatic hyperplasia (BPH) aged from 51 to 89 years. Prostatilen proved its efficacy in relieving manifestations of infravesical obstruction in BPH, therefore, prostatilen administration in the treatment of patients with initial or moderate subjective and objective symptoms is justified. Dimexide suppositoria containing 30 mg of prostatilen are preferable.
Assuntos
Peptídeos/uso terapêutico , Hiperplasia Prostática/complicações , Hiperplasia Prostática/tratamento farmacológico , Transtornos Urinários/tratamento farmacológico , Idoso , Idoso de 80 Anos ou mais , Humanos , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento , Transtornos Urinários/etiologiaRESUMO
Thermal activation of transient receptor potential (TRP) cation channels is one of the most striking examples of temperature-controlled processes in cell biology. As the evidence indicating the fundamental role of such processes in thermosensation builds at a fast pace, adequately accurate tools that would allow heat receptor logic behind thermosensation to be examined on a single-cell level are in great demand. Here, we demonstrate a specifically designed fiber-optic probe that enables thermal activation with simultaneous online thermometry of individual cells expressing genetically encoded TRP channels. This probe integrates a fiber-optic tract for the delivery of laser light with a two-wire microwave transmission line. A diamond microcrystal fixed on the fiber tip is heated by laser radiation transmitted through the fiber, providing a local heating of a cell culture, enabling a well-controlled TRP-assisted thermal activation of cells. Online local temperature measurements are performed by using the temperature-dependent frequency shift of optically detected magnetic resonance, induced by coupling the microwave field, delivered by the microwave transmission line, to nitrogen--vacancy centers in the diamond microcrystal. Activation of TRP channels is verified by using genetically encoded fluorescence indicators, visualizing an increase in the calcium flow through activated TRP channels.
Assuntos
Tecnologia de Fibra Óptica/métodos , Fibras Ópticas , Temperatura , Termometria/métodos , Animais , Cálcio/metabolismo , Diamante/química , Tecnologia de Fibra Óptica/instrumentação , Humanos , Lasers , Lógica , Espectroscopia de Ressonância Magnética/instrumentação , Espectroscopia de Ressonância Magnética/métodos , Micro-Ondas , Reprodutibilidade dos Testes , Análise de Célula Única/instrumentação , Análise de Célula Única/métodos , Termometria/instrumentação , Canais de Potencial de Receptor Transitório/genética , Canais de Potencial de Receptor Transitório/metabolismoRESUMO
Rabbits were exposed to vertically directed UV-B radiation (280-320 nm) 5 days a week during 10 months with daily dose 6.5 J/m2 (biological effective daily dose 0.83 J/m2). Within the first week expressed photokeratoconjunctivitis was observed, but to the end of third week state of cornea and conjunctiva became practically normal. Transparency of intra-eye structures was unchanged, functional activity of retina underwent undulatory alterations. Amplitude changes of a- and b-waves of electroretinogram reached 30% of control values during the first, were negligible within the second, and exceed control by 15-20% between 8 and 10-th months of exposure. Critical frequency of flashing was unchanged.
Assuntos
Eletrorretinografia/efeitos da radiação , Olho/efeitos da radiação , Raios Ultravioleta/efeitos adversos , Adaptação Fisiológica/efeitos da radiação , Animais , Olho/fisiopatologia , Fusão Flicker/efeitos da radiação , Ceratoconjuntivite/etiologia , Ceratoconjuntivite/fisiopatologia , Coelhos , Lesões Experimentais por Radiação/etiologia , Lesões Experimentais por Radiação/fisiopatologia , Fatores de TempoRESUMO
No essential changes occurred in the morphology, viability and agglutinability of 212 Leptospira strains belonging to 20 serological groups during 2-year storage in ampoules at temperatures of -60 to -70 degrees C, and 18 to 24 degrees C. After storage lasting 3 and 4 years viability was retained, respectively, by 95.75 and 78.5% of the strains stored at a temperature of -60 to -70 degrees C and by 90.9 and 70% of the strains stored at room temperature. During 4 years of storage at room temperature the agglutinability of the strains decreased 2-8 times. Most of the strains retained their virulence unchanged for 2 years at a temperature of -60 to -70 degrees C.
Assuntos
Leptospira/patogenicidade , Preservação Biológica , Temperatura , Testes de Aglutinação , Animais , Cricetinae , Dose Letal Mediana , Mesocricetus , Fatores de Tempo , VirulênciaRESUMO
The eyes of rabbits were UV-irradiated in a range close to the natural spectrum at a dose of 23.2 kJ/m2 (biologically effective dose 1.3 kJ/m2). The condition of optic media was estimated, as well as the parameters of electroretinogram (ERG) induced by single light stimulus of varying intensity and paired light stimuli with different intervals between them. As a result a distinct photokeratoconjunctivitis developed, the amplitude of the ERG a-wave decreased by 30-50% and that of b-wave by 35%, while the latencies of the both waves and the critical frequency of flashes remained unchanged. The cornea transparency did not decrease markedly even during the period of maximum photokeratoconjunctivitis and the changes in the ERG parameters were mostly due to the retina damage by the indirect effect of UV irradiation.