Bending the curve: Simple but massive conservation action leads to landscape-scale recovery of amphibians.

Success stories are rare in conservation science, hindered also by the research-implementation gap, where scientific insights rarely inform practice and practical implementation is rarely evaluated scientifically. Amphibian population declines, driven by multiple stressors, are emblematic of the freshwater biodiversity crisis. Habitat creation is a straightforward conservation action that has been shown to locally benefit amphibians, as well as other taxa, but does it benefit entire amphibian communities at large spatial scales? Here, we evaluate a landscape-scale pond-construction program by fitting dynamic occupancy models to 20 y of monitoring data for 12 pond-breeding amphibian species in the Swiss state Aargau, a densely populated area of the Swiss lowlands with intensive land use. After decades of population declines, the number of occupied ponds increased statewide for 10 out of 12 species, while one species remained stable and one species further declined between 1999 and 2019. Despite regional differences, in 77% of all 43 regional metapopulations, the colonization and subsequent occupation of new ponds stabilized (14%) or increased (63%) metapopulation size. Likely mechanisms include increased habitat availability, restoration of habitat dynamics, and increased connectivity between ponds. Colonization probabilities reflected species-specific preferences for characteristics of ponds and their surroundings, which provides evidence-based information for future pond construction targeting specific species. The relatively simple but landscape-scale and persistent conservation action of constructing hundreds of new ponds halted declines and stabilized or increased the state-wide population size of all but one species, despite ongoing pressures from other stressors in a human-dominated landscape.

Climate regulation processes are linked to the functional composition of plant communities in European forests, shrublands, and grasslands.

Terrestrial ecosystems affect climate by reflecting solar irradiation, evaporative cooling, and carbon sequestration. Yet very little is known about how plant traits affect climate regulation processes (CRPs) in different habitat types. Here, we used linear and random forest models to relate the community-weighted mean and variance values of 19 plant traits (summarized into eight trait axes) to the climate-adjusted proportion of reflected solar irradiation, evapotranspiration, and net primary productivity across 36,630 grid cells at the European extent, classified into 10 types of forest, shrubland, and grassland habitats. We found that these trait axes were more tightly linked to log evapotranspiration (with an average of 6.2% explained variation) and the proportion of reflected solar irradiation (6.1%) than to net primary productivity (4.9%). The highest variation in CRPs was explained in forest and temperate shrubland habitats. Yet, the strength and direction of these relationships were strongly habitat-dependent. We conclude that any spatial upscaling of the effects of plant communities on CRPs must consider the relative contribution of different habitat types.

Building pondscapes for amphibian metapopulations.

The success of ponds constructed to restore ecological infrastructure for pond-breeding amphibians and benefit aquatic biodiversity depends on where and how they are built. We studied effects of pond and landscape characteristics, including connectivity, on metapopulation dynamics of 12 amphibian species in Switzerland. To understand the determinants of long-term occupancy (here summarized as incidence), environmental effects on both colonization and persistence should be considered. We fitted dynamic occupancy models to 20 years of monitoring data on a pond construction program to quantify effects of pond and landscape characteristics and different connectivity metrics on colonization and persistence probabilities in constructed ponds. Connectivity to existing populations explained dynamics better than structural connectivity metrics, and simple metrics (distance to the nearest neighbor population, population density) were useful surrogates for dispersal kernel-weighted metrics commonly used in metapopulation theory. Population connectivity mediated the persistence of conservation target species in new ponds, suggesting source-sink dynamics in newly established populations. Population density captured this effect well and could be used by practitioners for site selection. Ponds created where there were 2-4 occupied ponds within a radius of ∼0.5 km had >3.5 times higher incidence of target species (median) than isolated ponds. Species had individual preferences regarding pond characteristics, but breeding sites with larger (≥100 m2) total water surface area, that temporarily dried, and that were in surroundings with maximally 50% forest benefitted multiple target species. Pond diversity will foster amphibian diversity at the landscape scale.

Construcción de estanques para meta poblaciones de anfibios Resumen El éxito de los estanques construidos para restaurar la infraestructura ecológica para los anfibios que allí se reproducen y para beneficiar la biodiversidad acuática depende de en dónde y cómo se construyen. Estudiamos los efectos de las características de los estanques y el paisaje, incluida la conectividad, sobre la dinámica de las meta poblaciones de 12 especies de anfibios en Suiza. Se deben considerar los efectos ambientales sobre la colonización y la persistencia para entender las determinantes de la ocupación a largo plazo (resumida aquí como incidencia). Ajustamos los modelos dinámicos de ocupación a datos de 20 años de monitoreo de un programa de construcción de estanques para cuantificar los efectos de las características del estanque y el paisaje y las diferentes medidas de conectividad para las probabilidades de colonización y persistencia en los estanques construidos. La conectividad con las poblaciones existentes explicó mejor la dinámica que las medidas de conectividad estructural, mientras que las medidas simples (distancia a la población vecina más cercana, densidad poblacional) fueron sustitutos útiles para las medidas de dispersión ponderadas al núcleo que se usan con frecuencia en la teoría de meta poblaciones. La conectividad poblacional medió la persistencia de las especies a conservar en los estanques nuevos, lo que sugiere que hay dinámicas fuente­sumidero en las poblaciones recién establecidas. La densidad poblacional capturó muy bien este efecto y podría usarse para que los practicantes seleccionen sitios. Los estanques construidos en un radio de ≈0.5 km de dos a cuatro estanques ocupados tuvieron >3.5 más incidencia de las especies a conservar (mediana) que los estanques aislados. Las especies tuvieron preferencias individuales con respecto a las características de los estanques, aunque los sitios de reproducción con una mayor superficie total de agua (≥100 m2), que se secaban temporalmente y que estaban rodeados con un máximo de 50% de bosque beneficiaron a muchas especies a conservar. Por esto, la diversidad de estanques promoverá la diversidad de anfibios a escala de paisaje.

Estimation of breeding probability can make monitoring data more revealing: a case study of amphibians.

Monitoring programs serve to detect trends in the distribution and abundance of species. To do so, monitoring programs often use static state variables. Dynamic state variables that describe population dynamics might be more valuable because they allow for a mechanistic understanding of the processes that lead to population trends. We fit multistate occupancy models to data from a country-wide multispecies amphibian occupancy monitoring program and estimated occupancy and breeding probabilities. If breeding probabilities are determinants of occupancy dynamics, then they may serve in monitoring programs as state variables that describe dynamic processes. The results showed that breeding probabilities were low and that a large proportion of the populations had to be considered to be non-breeding populations (i.e., populations where adults are present but no breeding occurs). For some species, the majority of populations were non-breeding populations. We found that non-breeding populations have lower persistence probabilities than populations where breeding occurs. Breeding probabilities may thus explain trends in occupancy but they might also explain other ecological phenomena, such as the success of invasive species, which had high breeding probabilities. Signs of breeding, i.e., the presence of eggs and larvae, were often hard to detect. Importantly, non-breeding populations also had low detection probabilities, perhaps because they had lower abundances. We suggest that monitoring programs should invest more in the detection of life history stages indicative of breeding, and also into the detection of non-breeding populations. We conclude that breeding probability should be used as a state variable in monitoring programs because it can lead to deeper insights into the processes driving occupancy dynamics.

Unravelling the impact of soil data quality on species distribution models of temperate forest woody plants.

Soil properties influence plant physiology and growth, playing a fundamental role in shaping species niches in temperate forest ecosystems. Here, we investigated the impact of soil data quality on the performance of species distribution models (SDMs) of 41 woody plant species in Swiss forests. We compared models based on measured soil properties with those based on digitally mapped soil properties on regional (Swiss Forest Soil Maps) and global scales (SoilGrids). We first calibrated topo-climatic SDMs with measured soil data and plant species presences and absences from mature temperate forest stand plots. We developed further models using the same soil predictors, but with values extracted from digital soil maps at the nearest neighbouring plots of the Swiss National Forestry Inventory. The predictive power of SDMs without soil information compared to those with soil information, as well as measured soil information vs digitally mapped, was evaluated with metrics of model performance and variable contribution. On average, models with measured and digitally mapped soil properties performed significantly better than those without soil information. SDMs based on measured and Swiss Forest Soil Maps showed higher performance, especially for species with an 'extreme' niche position (e.g., preference for high or low pH), compared to those using SoilGrids. Nevertheless, if no regional soil maps are available, SoilGrids should be tested for their potential to improve SDMs. Moreover, among the tested soil predictors, pH, and clay content of the topsoil layers most improved the predictive power of SDMs for forest woody plants. In conclusion, we demonstrate the value of regional soil maps for predicting the distribution of woody species across strong environmental gradients in temperate forests. The improved accuracy of SDMs and insights into drivers of distribution may support forest managers in strategies supporting e.g. biodiversity conservation, or climate adaptation planning.

Agricultural practices and biodiversity: Conservation policies for semi-natural grasslands in Europe.

Europe's semi-natural grasslands support notably high levels of temperate biodiversity across multiple taxonomic groups. However, these ecosystems face unique conservation challenges. Contemporary agricultural practices have replaced historical traditional low-intensity agriculture in many regions, resulting in a spectrum of management intensities within these ecosystems, ranging from highly intensive methods to complete abandonment. Paradoxically, both extremes along this spectrum of management intensity can be detrimental to biodiversity of semi-natural grasslands. Moreover, while anthropogenic climate change is an overarching threat to these ecosystems, rapid changes in land use and its intensity often present more immediate pressures. Often occurring at a faster rate than climate change itself, these land-use changes have the potential to rapidly impact the biodiversity of these grasslands. Here, we divide the ecological processes, threats, and developments to semi-natural grasslands into three sections. First, we examine the different impacts of agricultural intensification and abandonment on these ecosystems, considering their different consequences for biodiversity. Second, we review seminal works on various evidence-based management practices and offer a concise summary that provides support for various conservation and management strategies. However, the socio-economic factors that drive both abandonment and intensification in semi-natural grasslands can also be used to develop solutions through strategic governmental and non-governmental interventions. Accordingly, we conclude with a way forward by providing several key policy recommendations. By synthesizing existing knowledge and identifying research gaps, this essay aims to provide valuable insights for advancing the sustainable management of semi-natural grasslands.

Characterization of nuclear microsatellite loci in the calcareous fen specialist Scorpidium cossonii (Calliergonaceae).

PREMISE OF THE STUDY: Nuclear microsatellite markers were developed in the fen specialist moss, Scorpidium cossonii, to study genetic diversity and genetic structure of this species in relation to land-use types, habitat fragmentation, and habitat conservation measures. METHODS AND RESULTS: The polymorphisms of 14 microsatellite markers were characterized. All primers were tested on 140 gametophytes collected from four populations in Switzerland. The primers amplified di- and trinucleotide repeats with three to 26 alleles per locus. The primers worked also in the two other species of the genus: 14 and 12 primers successfully amplified in S. revolvens and S. scorpioides, respectively. CONCLUSIONS: In future studies, our primers have the potential to provide valuable information on genetic diversity, genetic structure, and on historical and recent gene flow in S. cossonii; they should also enable related research in other Scorpidium species.

The distribution of climbing chalk on climbed boulders and its impact on rock-dwelling fern and moss species.

Rock climbing is popular, and the number of climbers rises worldwide. Numerous studies on the impact of climbing on rock-dwelling plants have reported negative effects, which were mainly attributed to mechanical disturbances such as trampling and removal of soil and vegetation. However, climbers also use climbing chalk (magnesium carbonate hydroxide) whose potential chemical effects on rock-dwelling species have not been assessed so far. Climbing chalk is expected to alter the pH and nutrient conditions on rocks, which may affect rock-dwelling organisms. We elucidated two fundamental aspects of climbing chalk. (a) Its distribution along nonoverhanging climbing routes was measured on regularly spaced raster points on gneiss boulders used for bouldering (ropeless climbing at low height). These measurements revealed elevated climbing chalk levels even on 65% of sampling points without any visual traces of climbing chalk. (b) The impact of climbing chalk on rock-dwelling plants was assessed with four fern and four moss species in an experimental setup in a climate chamber. The experiment showed significant negative, though varied effects of elevated climbing chalk concentrations on the germination and survival of both ferns and mosses. The study thus suggests that along climbing routes, elevated climbing chalk concentration can occur even were no chalk traces are visible and that climbing chalk can have negative impacts on rock-dwelling organisms.

Evidence for a possible extinction debt in Swiss wetland specialist plants.

Habitat loss leading to smaller patch sizes and decreasing connectivity is a major threat to global biodiversity. While some species vanish immediately after a change in habitat conditions, others show delayed extinction, that is, an extinction debt. In case of an extinction debt, the current species richness is higher than expected under present habitat conditions.We investigated wetlands of the canton of Zürich in the lowlands of Eastern Switzerland where a wetland loss of 90% over the last 150 years occurred. We related current species richness to current and past patch area and connectivity (in 1850, 1900, 1950, and 2000). We compared current with predicted species richness in wetlands with a substantial loss in patch area based on the species-area relationship of wetlands without substantial loss in patch area and studied relationships between the richness of different species groups and current and historical area and connectivity of wetland patches.We found evidence of a possible extinction debt for long-lived wetland specialist vascular plants: in wetlands, which substantially lost patch area, current species richness of long-lived specialist vascular plants was higher than would have been expected based on current patch area. Additionally and besides current wetland area, historical area also explained current species richness of these species in a substantial and significant way. No evidence for an extinction debt in bryophytes was found.The possible unpaid extinction debt in the wetlands of the canton of Zürich is an appeal to nature conservation, which has the possibility to prevent likely future extinctions of species through specific conservation measures. In particular, a further reduction in wetlands must be prevented and restoration measures must be taken to increase the number of wetlands.

Epiphytes in wooded pastures: Isolation matters for lichen but not for bryophyte species richness.

Sylvo-pastoral systems are species-rich man-made landscapes that are currently often severely threatened by abandonment or management intensification. At low tree densities, single trees in these systems represent habitat islands for epiphytic cryptogams. Here, we focused on sycamore maple (Acer pseudoplatanus) wooded pastures in the northern European Alps. We assessed per tree species richness of bryophytes and lichens on 90 sycamore maple trees distributed across six study sites. We analysed the effects of a range of explanatory variables (tree characteristics, environmental variables and isolation measures) on the richness of epiphytic bryophytes and lichens and various functional subgroups (based on diaspore size, habitat preference and red list status). Furthermore, we estimated the effect of these variables on the occurrence of two specific bryophyte species (Tayloria rudolphiana, Orthotrichum rogeri) and one lichen species (Lobaria pulmonaria) of major conservation concern. Bryophytes and lichens, as well as their subgroups, were differently and sometimes contrastingly affected by the variables considered: tree diameter at breast height had no significant effect on bryophytes but negatively affected many lichen groups; tree phenological age positively affected red-listed lichens but not red-listed bryophytes; increasing isolation from neighbouring trees negatively affected lichens but not bryophytes. However, the high-priority bryophyte species T. rudolphiana was also negatively affected by increased isolation at small spatial scales. Orthotrichum rogeri was more frequent on young trees and L. pulmonaria was more frequent on trees with thin stems and large crowns. The results indicate that local dispersal is important for lichens, whereas long distance dispersal seems to be more important for colonisation by bryophytes. Furthermore, our study highlights that different conservation measures need to be taken depending on the taxonomic and functional species group or the individual species that is addressed. In practice, for the conservation of a high overall richness in sylvo-pastoral systems, it is crucial to sustain not only old and large trees but rather a wide range of tree sizes and ages.