RESUMO
Patterns of phenotypic and genic frequencies across hybrid zones provide insight into the origin and evolution of reproductive isolation. The Reunion grey white-eye, Zosterops borbonicus, exhibits parapatrically distributed plumage colour forms across the lowlands of the small volcanic island of Reunion (Mascarene archipelago). These forms meet and hybridize in regions that are natural barriers to dispersal (rivers, lava fields). Here, we investigated the relationship among patterns of differentiation at neutral genetic (microsatellite) markers, phenotypic traits (morphology and plumage colour) and niche characteristics across three independent hybrid zones. Patterns of phenotypic divergence revealed that these hybrid zones are among the narrowest ever documented in birds. However, the levels of phenotypic divergence stand in stark contrast to the lack of clear population neutral genetic structure between forms. The position of the hybrid zones coincides with different natural physical barriers, yet is not associated with steep changes in vegetation and related climatic variables, and major habitat transitions are shifted from these locations by at least 18 km. This suggests that the hybrid zones are stabilized over natural dispersal barriers, independently of environmental boundaries, and are not associated with niche divergence. A striking feature of these hybrid zones is the very low levels of genetic differentiation in neutral markers between forms, suggesting that phenotypic divergence has a narrow genetic basis and may reflect recent divergence at a few linked genes under strong selection, with a possible role for assortative mating in keeping these forms apart.
Assuntos
Passeriformes/genética , Animais , Frequência do Gene , Hibridização Genética , Ilhas , Repetições de Microssatélites , Passeriformes/anatomia & histologia , Fenótipo , Isolamento ReprodutivoRESUMO
Adaptation to local environmental conditions and the range dynamics of populations can influence evolutionary divergence along environmental gradients. Thus, it is important to investigate patterns of both phenotypic and genetic variations among populations to reveal the respective roles of these two types of factors in driving population differentiation. Here, we test for evidence of phenotypic and genetic structure across populations of a passerine bird (Zosterops borbonicus) distributed along a steep elevational gradient on the island of Réunion. Using 11 microsatellite loci screened in 401 individuals from 18 localities distributed along the gradient, we found that genetic differentiation occurred at two spatial levels: (i) between two main population groups corresponding to highland and lowland areas, respectively, and (ii) within each of these two groups. In contrast, several morphological traits varied gradually along the gradient. Comparison of neutral genetic differentiation (FST ) and phenotypic differentiation (PST ) showed that PST largely exceeds FST at several morphological traits, which is consistent with a role for local adaptation in driving morphological divergence along the gradient. Overall, our results revealed an area of secondary contact midway up the gradient between two major, cryptic, population groups likely diverged in allopatry. Remarkably, local adaptation has shaped phenotypic differentiation irrespective of population history, resulting in different patterns of variation along the elevational gradient. Our findings underscore the importance of understanding both historical and selective factors when trying to explain variation along environmental gradients.
Assuntos
Altitude , Variação Genética , Passeriformes/fisiologia , Seleção Genética , Adaptação Fisiológica/genética , Animais , Evolução Biológica , Ilhas , Repetições de Microssatélites/genética , Passeriformes/genética , FenótipoRESUMO
OBJECTIVES: Myotonic dystrophy type 1 (DM1) is the most common adult-onset muscular dystrophy. It is associated with motor symptoms but patients also display non-motor symptoms such as particular personality traits. Studies have reported mixed results about personality characteristics which may be attributable to small sample sizes, different disease severity of groups studied, and use of different questionnaires or method. This study aimed to describe the psychological characteristics of a large cohort of patients with DM1, to characterize those at risk of developing a psychiatric disorder, and to compare characteristics between two DM1 phenotypes, a mild and more severe adult-onset phenotype. METHODS: Two hundred patients with DM1 (152 adult-onset; 48 mild) were asked to complete questionnaires assessing personality traits, psychological symptoms, self-esteem, and suicidal risk. Neurological and neuropsychological assessments were performed to compare personality characteristics to clinical and cognitive measures. RESULTS: Patients with DM1 globally showed personality traits and psychological symptoms in the average range compared to normative data, with normal levels of self-esteem and suicidal ideation. However, 27% of patients were found to be at high risk of developing a psychiatric disorder. Moreover, psychological traits differed across phenotypes, with the most severe phenotype tending to show more severe psychological symptoms. The presence of higher phobic anxiety and lower self-esteem was associated with lower education, a higher number of CTG repeats, more severe muscular impairment, and lower cognitive functioning (P < 0.001). CONCLUSIONS: Different phenotypes should thus be taken into account in clinical settings for individual management of patients and optimizing therapeutic success.
Assuntos
Distrofia Miotônica/psicologia , Adulto , Feminino , Humanos , Masculino , Transtornos Mentais/etiologia , Pessoa de Meia-Idade , Distrofia Miotônica/complicações , Testes Neuropsicológicos , Fenótipo , Inquéritos e QuestionáriosRESUMO
The Réunion grey white-eye, Zosterops borbonicus, a passerine bird endemic to Réunion Island in the Mascarene archipelago, represents an extreme case of microgeographical plumage colour variation in birds, with four distinct colour forms occupying different parts of this small island (2512 km(2)). To understand whether such population differentiation may reflect low levels of dispersal and gene flow at a very small spatial scale, we examined population structure and gene flow by analysing variation at 11 microsatellite loci among four geographically close localities (<26 km apart) sampled within the distribution range of one of the colour forms, the brown-headed brown form. Our results revealed levels of genetic differentiation that are exceptionally high for birds at such a small spatial scale. This strong population structure appears to reflect low levels of historical and contemporary gene flow among populations, unless very close geographically (<10 km). Thus, we suggest that the Réunion grey white-eye shows an extremely reduced propensity to disperse, which is likely to be related to behavioural processes.
Assuntos
Aves/genética , Fluxo Gênico , Alelos , Animais , Análise por Conglomerados , Evolução Molecular , Feminino , Loci Gênicos , Variação Genética , Genética Populacional , Genótipo , Geografia , Endogamia , Ilhas , Masculino , Repetições de Microssatélites , MutaçãoRESUMO
The electrical Ca test was used to measure H2O vapor transmission through polyethylene naphthalate (PEN) polymer with a thickness of 200 µm. On the basis of the time required for the normalized conductance of the Ca film to reach zero, the H2O vapor transmission rate was determined versus H2O flux, temperature, and saturation of the PEN polymer with H2O. The H2O vapor transmission rate was proportional to the H2O flux and only weakly dependent on temperature at constant H2O flux. The transmission coefficient, Γ, for H2O through the PEN polymer at 70 °C was Γ â¼ 3.2 × 10(-10). The corresponding water vapor transmission rate (WVTR) at 70 °C/80% RH was 0.65 g/(m(2) day). The temperature dependence of the H2O vapor transmission rate through PEN at constant H2O flux yielded an activation barrier of E = 12.4 kJ/mol. There was no observable reservoir effect for H2O in the PEN polymer. The H2O vapor transmission rates for initially dry or H2O-saturated PEN polymer substrates were nearly identical at various temperatures. Although the time required for the normalized conductance of the Ca film to reach zero was inversely proportional to the H2O flux, the Ca film conductance did not decrease linearly versus H2O exposure. The Ca film conductance changed very little during initial H2O exposure. This behavior may be caused by the nonlinear oxidation kinetics of the Ca film.
RESUMO
BACKGROUND: It is unclear whether cognitive skill deficits during childhood carry risk for suicide attempt or mortality later in adulthood at the population level. We conducted a systematic review and meta-analysis of population-based studies examining the association between childhood cognitive skills and adult suicidal behavior, namely attempt and mortality. METHOD: We systematically searched databases for articles then extracted study characteristics and estimates on the association between childhood cognitive skills (i.e., IQ or school performance at age ≤ 18 years) and later suicide attempt and mortality. Random-effect meta-analysis was used to quantify this association across all studies with available data. RESULTS: Twenty-three studies met the inclusion criteria and suggest an association between lower childhood cognitive skills and increased risk of suicidal behavior. Meta-analysis of the adjusted estimates from 11 studies (N = 2,830,191) found the association to be small but statistically significant. Heterogeneity was significant but moderate, and results were unlikely to be influenced by publication bias. In subgroup analyses, associations were significant only for males. No difference in effect size was found between suicide attempt and suicide mortality. LIMITATIONS: Cognitive skills were measured with different cognitive subtests. Heterogeneity in the age of cognitive skills assessment. Meta-regression and subgroup analyses were based on a relatively low number of studies. CONCLUSIONS: Individuals with lower cognitive skills in childhood have a greater risk of suicidal behavior in adulthood, especially males. Although the association was small, interventions improving cognitive skills may yield large effects on suicide prevention at the population level if the association is causal.
Assuntos
Ideação Suicida , Tentativa de Suicídio , Masculino , Criança , Humanos , Adulto , Adolescente , Tentativa de Suicídio/psicologia , Prevenção do Suicídio , Comportamento Infantil , CogniçãoRESUMO
Late-life depression remains an underdiagnosed clinical entity, mainly because the presence of cognitive impairment in the elderly leads clinicians to suspect dementia rather than depression. Our objective was to analyze the cognitive abilities of elderly depressed patients using the Montreal Cognitive Assessment (MoCA) in relation to the presence or absence of suicidal ideation. The MoCA, Beck Scale of Suicidal Ideation, Hamilton Anxiety Scale, and Hamilton Depression Scale were administered to 72 patients with a recent history of late life depression: 43 with suicidal ideation and 29 non-suicidal controls. The results show that suicidal patients demonstrated significantly worse performance on the MoCA total score and the delayed recall subtest in comparison to non-suicidal controls. In addition, after adjusting for age and depression, poorer performance on the MoCA total score correlated to the presence of suicidal ideation. We found that the MoCA total score is able to predict the presence of suicidal ideation in depressed elderly patients in a fair-to-good manner. As late-life depression is already established as a potential prodrome of dementia, longitudinal follow-up may determine whether depressed individuals with suicidal ideation are at higher risk of converting to dementia.
Assuntos
Disfunção Cognitiva , Ideação Suicida , Idoso , Depressão , Humanos , Testes de Estado Mental e DemênciaRESUMO
We have shown previously that a maternal junk food diet during pregnancy and lactation plays a role in predisposing offspring to obesity. Here we show that rat offspring born to mothers fed the same junk food diet rich in fat, sugar and salt develop exacerbated adiposity accompanied by raised circulating glucose, insulin, triglyceride and/or cholesterol by the end of adolescence (10 weeks postpartum) compared with offspring also given free access to junk food from weaning but whose mothers were exclusively fed a balanced chow diet in pregnancy and lactation. Results also showed that offspring from mothers fed the junk food diet in pregnancy and lactation, and which were then switched to a balanced chow diet from weaning, exhibited increased perirenal fat pad mass relative to body weight and adipocyte hypertrophy compared with offspring which were never exposed to the junk food diet. This study shows that the increased adiposity was more enhanced in female than male offspring and gene expression analyses showed raised insulin-like growth factor-1 (IGF-1), insulin receptor substrate (IRS)-1, vascular endothelial growth factor (VEGF)-A, peroxisome proliferator-activated receptor-gamma (PPARgamma), leptin, adiponectin, adipsin, lipoprotein lipase (LPL), Glut 1, Glut 3, but not Glut 4 mRNA expression in females fed the junk food diet throughout the study compared with females never given access to junk food. Changes in gene expression were not as marked in male offspring with only IRS-1, VEGF-A, Glut 4 and LPL being up-regulated in those fed the junk food diet throughout the study compared with males never given access to junk food. This study therefore shows that a maternal junk food diet promotes adiposity in offspring and the earlier onset of hyperglycemia, hyperinsulinemia and/or hyperlipidemia. Male and female offspring also display a different metabolic, cellular and molecular response to junk-food-diet-induced adiposity.
Assuntos
Adiposidade , Dieta , Fenômenos Fisiológicos da Nutrição Pré-Natal , Caracteres Sexuais , Animais , Glicemia , Colesterol/sangue , Feminino , Perfilação da Expressão Gênica , Insulina/sangue , Lactação , Obesidade , Gravidez , Ratos , Transcrição Gênica , Triglicerídeos/sangue , DesmameRESUMO
BACKGROUND: Major depressive episode (MDE) has been associated with cognitive functioning alteration and hypovitaminosis D (hypoVD), but the relationship between hypoVD, depression, and cognition is not well understood. We aimed to compare patient with MDE with or without hypoVD in regard of cognitive functioning. METHODS: 91 patients (38.5 years old, 65.9% female) with MDE were included in a cross-sectional study and were evaluated with a complete cognitive battery. None of the participants were medicated at the time of the inclusion. Serum 25-hydroxyvitamin D was measured using LC-MS/MS method, and hypovitaminosis was defined as 25OHD < 50nmol/L. Covariates were gender, season of dosage, first MDE onset, age, body mass index and depression severity RESULTS: Patients with hypoVD demonstrated a higher stroop intereference index time underscoring that means low cognitive inhibition ability. Mutiple logistic regression confirmed that hypoVD was significantly associated with high stroop interference time index after controlling by gender, season of dosage, first MDE onset, age, body mass index and depression severity. CONCLUSION: Our results suggest that patient with MDE having hypoVD may be more prone to cognitive impairment.
Assuntos
Transtornos Cognitivos/etiologia , Transtorno Depressivo Maior/complicações , Deficiência de Vitamina D/etiologia , Adolescente , Adulto , Idoso , Cognição/fisiologia , Transtornos Cognitivos/psicologia , Estudos Transversais , Depressão/psicologia , Transtorno Depressivo Maior/psicologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estações do Ano , Espectrometria de Massas em Tandem , Vitamina D/análogos & derivados , Vitamina D/sangue , Adulto JovemRESUMO
The objective of this study was to determine if feeding carbohydrate supplements with faster degradation rates than corn to dairy cows grazing ryegrass would improve nitrogen capture, milk production, and components. Treatments were grain supplements based on: 1) corn (CORN), 2) barley and molasses (BM), or 3) citrus pulp and molasses (CM). For BM and CM, the diet composition was the same as that of CORN except that a portion of the corn was replaced with barley and molasses or citrus pulp and molasses, respectively, on a dry matter basis. Cows grazed ryegrass (Lolium multiflorum Lam.) pasture. Yield of milk, 3.5% fat-corrected milk, energy-corrected milk, and milk fat, as well as milk fat percentage, were not different among treatments. True milk protein percentage was higher for CORN (2.81%) compared with CM (2.70%), but was not different for BM (2.77%). However, true milk protein yield was not different among treatments. Milk urea N was higher for BM (11.43 mg/dL) compared with both CORN and CM (average: 9.95 mg/dL). There were no differences among CORN, BM, and CM treatments for overall BUN (average: 10.60 mg/dL). At 0400 h, however, cows on CORN had higher BUN than cows on CM (11.43 vs. 9.96 mg/dL), but there were no differences between CORN and BM (average: 11.21 mg/dL) or BM and CM (average: 10.48 mg/dL), and there were no differences among treatments at other time points. The CM diet might have shown more advantage if the pasture crude protein content was higher. Partial replacement of corn with citrus pulp for grazing cows should be further studied using pasture with higher crude protein content. Although cows receiving CM and BM did not produce more milk than cows on CORN, if barley or citrus pulp is less expensive than corn, they may be viable replacements for a portion of the corn supplement for grazing cows.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal , Bovinos/fisiologia , Carboidratos da Dieta/administração & dosagem , Carboidratos da Dieta/metabolismo , Nitrogênio/metabolismo , Animais , Citrus , Dieta , Gorduras/análise , Feminino , Frutas , Hordeum , Lactação , Lolium , Leite/química , Melaço , Nitrogênio/análise , Ureia/análise , Zea maysRESUMO
GSK-3beta is a regulatory serine/threonine kinase with a plethora of cellular targets. Consequently, selective small molecule inhibitors of GSK-3beta may have a variety of therapeutic uses including the treatment of neurodegenerative diseases, type II diabetes and cancer. In order to characterize the active site of GSK-3beta, we determined crystal structures of unphosphorylated GSK-3beta in complex with selective and non-selective ATP-mimetic inhibitors. Analysis of the inhibitors' interactions with GSK-3beta in the structures reveals how the enzyme can accommodate a number of diverse molecular scaffolds. In addition, a conserved water molecule near Thr138 is identified that can serve a functional role in inhibitor binding. Finally, a comparison of the interactions made by selective and non-selective inhibitors highlights residues on the edge of the ATP binding-site that can be used to obtain inhibitor selectivity. Information gained from these structures provides a promising route for the design of second-generation GSK-3beta inhibitors.
Assuntos
Trifosfato de Adenosina/metabolismo , Inibidores Enzimáticos/farmacologia , Quinase 3 da Glicogênio Sintase/química , Mimetismo Molecular , Antibióticos Antineoplásicos/farmacologia , Benzazepinas/farmacologia , Sítios de Ligação , Ligação Competitiva , Proteína Quinase CDC2/metabolismo , Cristalografia por Raios X , Quinase 3 da Glicogênio Sintase/antagonistas & inibidores , Quinase 3 da Glicogênio Sintase/metabolismo , Glicogênio Sintase Quinase 3 beta , Inibidores do Crescimento/farmacologia , Humanos , Indóis/farmacologia , Maleimidas/farmacologia , Fosforilação , Conformação Proteica , Receptores Proteína Tirosina Quinases/química , Receptores Proteína Tirosina Quinases/metabolismo , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos , Receptores de Fatores de Crescimento de Fibroblastos/química , Receptores de Fatores de Crescimento de Fibroblastos/metabolismo , Estaurosporina/farmacologia , Relação Estrutura-AtividadeRESUMO
UDP-N-acetylmuramoyl-l-alanine:d-glutamate (MurD) ligase catalyses the addition of d-glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-l-alanine (UMA). The crystal structures of Escherichia coli in the substrate-free form and MurD complexed with UMA have been determined at 2.4 A and 1.88 A resolution, respectively. The MurD structure comprises three domains each of a topology reminiscent of nucleotide-binding folds. In the two structures the C-terminal domain undergoes a large rigid-body rotation away from the N-terminal and central domains. These two "open" structures were compared with the four published "closed" structures of MurD. In addition the comparison reveals which regions are affected by the binding of UMA, ATP and d-Glu. Also we compare and discuss two structurally characterized enzymes which belong to the same ligase superfamily: MurD and folylpolyglutamate synthetase (FGS). The analysis allows the identification of key residues involved in the reaction mechanism of FGS. The determination of the two "open" conformation structures represents a new step towards the complete elucidation of the enzymatic mechanism of the MurD ligase.
Assuntos
Escherichia coli/enzimologia , Peptídeo Sintases/química , Peptídeo Sintases/metabolismo , Uridina Difosfato Ácido N-Acetilmurâmico/análogos & derivados , Trifosfato de Adenosina/metabolismo , Sítios de Ligação , Cristalografia por Raios X , Glucose/metabolismo , Modelos Moleculares , Movimento (Física) , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Relação Estrutura-Atividade , Uridina Difosfato Ácido N-Acetilmurâmico/metabolismoRESUMO
UDP -N- acetylmuramoyl- L -alanine: D -glutamate (MurD) ligase catalyses the addition of d -glutamate to the nucleotide precursor UDP -N- acetylmuramoyl- L -alanine (UMA). The crystal structures of three complexes of Escherichia coli MurD with a variety of substrates and products have been determined to high resolution. These include (1) the quaternary complex of MurD, the substrate UMA, the product ADP, and Mg2+, (2) the quaternary complex of MurD, the substrate UMA, the product ADP, and Mn2+, and (3) the binary complex of MurD with the product UDP - N- acetylmuramoyl- L -alanine- D -glutamate (UMAG). The reaction mechanism supported by these structures proceeds by the phosphorylation of the C-terminal carboxylate group of UMA by the gamma-phosphate group of ATP to form an acyl-phosphate intermediate, followed by the nucleophilic attack by the amino group of D-glutamate to produce UMAG. A key feature in the reaction intermediate is the presence of two magnesium ions bridging negatively charged groups.
Assuntos
Peptídeo Sintases/química , Peptídeo Sintases/metabolismo , Difosfato de Adenosina/metabolismo , Adenilil Imidodifosfato/química , Adenilil Imidodifosfato/metabolismo , Sequência de Aminoácidos , Cristalografia por Raios X , Dipeptídeos/química , Dipeptídeos/metabolismo , Hidrólise , Magnésio/química , Magnésio/metabolismo , Manganês/química , Manganês/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Conformação Proteica , Homologia de Sequência de Aminoácidos , Uridina Difosfato Ácido N-Acetilmurâmico/análogos & derivados , Uridina Difosfato Ácido N-Acetilmurâmico/química , Uridina Difosfato Ácido N-Acetilmurâmico/metabolismoRESUMO
Expanded clays bipillared with [Al(13)O(4)(OH)(24)(H(2)O)(12)](7+) ions and with hexameric Cu complexes such as M[(&mgr;-OH)Cu(&mgr;-OCH(2)CH(2)NEt(2))](6)(ClO(4))(3), or with M[(&mgr;-OH)Cu(&mgr;-OCH(2)CH(2)NEt(2))](6)(PF(6))(3) where M = Fe, Al, Ga, form microporous materials whose stability and microporosity depend mainly on the identity of the hexamer central metal atom. In fact, a general decrease in thermal stability, interlamellar heights, surface areas, and pore volumes was noted when, in the (Cu,M) hexamer, M changed from gallium to aluminum to iron. Mossbauer results have indicated that only Fe(3+) in octahedral coordination is present in the iron-containing bi-PILC samples (bi-PILC = bipillared interlayered clays). It is believed that metals such as Fe(3+) and Cu(2+) can interact with the interlamellar Keggin ions thereby decreasing the stability of the alumina pillars. In contrast, the intermediate Al(13)-PILC structure is least affected when the more stable Cr complex is used. Bi-PILC materials containing 2.7-3.4% Cr stable to 500 degrees C have been obtained. The low polarity of the chosen solvent (acetonitrile) appears to inhibit the back-exchange of the intermediate PILC's Keggin ions with the hexameric Cu complexes. Elemental analysis together with XRD results suggests that the primary intercalation pathway was diffusion or ion exchange when Cr[(&mgr;-OCH(3))(&mgr;-OCH(2)CH(2)NEt(2))CuCl](3) or M[(&mgr;-OH)Cu(&mgr;-OCH(2)CH(2)NEt(2))](6)(ClO(4))(3), respectively, was used. In all preparations, bi-PILC were produced containing complexes that suffered ligand losses during the synthesis reaction. Molecular scale AFM images have shown that these complexes can be found also outside the clay interlamellar space.
Assuntos
Testosterona/sangue , Sangue , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Radioimunoensaio , Fatores Sexuais , Cordão UmbilicalAssuntos
Radioimunoensaio , Testículo/anormalidades , Testosterona/sangue , Síndrome de Turner/sangue , Adolescente , Adulto , Animais , Criança , Pré-Escolar , Anormalidades Congênitas/sangue , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Métodos , Puberdade , Coelhos/imunologia , Fatores Sexuais , TrítioRESUMO
The objective of this study was to determine if the nutrient and gossypol contents and in vitro digestibility of 3 types of genetically modified whole cottonseed differed from traditional whole cottonseed. Samples of seed from traditional (no genetic modifications) and genetically modified varieties of cotton grown in 1999 and 2000 were analyzed. Genetic modifications included the insertion of genes to protect cotton from insect pests (Bt), and damage from glyphosate herbicides (RR), and from both (Bt/RR). Year effects were significant for in vitro dry matter (DM) digestibility, gossypol, DM, crude protein (CP), fat, neutral detergent fiber (NDF), acid detergent fiber (ADF), and ash. Higher rainfall resulted in higher CP, fat, and ash and lower NDF and gossypol. There were no differences among seed types for ground or whole seed digestibility, DM, CP, fat, NDF, ADF, ash, lignin, net energy for lactation, amino acids, total fatty acids, or seed index. Overall, the nutrient content and digestibility of varieties of genetically modified seed were similar to that of varieties of traditional whole cottonseed.
Assuntos
Bovinos/metabolismo , Óleo de Sementes de Algodão/química , Gossypium/genética , Gossipol/análise , Plantas Geneticamente Modificadas , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Óleo de Sementes de Algodão/administração & dosagem , Gorduras na Dieta/análise , Fibras na Dieta/análise , Digestão , Feminino , Gossipol/efeitos adversos , Lactação/metabolismo , Valor Nutritivo , Proteínas de Plantas/análise , ChuvaRESUMO
Objectives were to determine the effects of adding 3 g/d of Aspergillus oryzae fermentation extract to diets with or without 5.6% added tallow. Twenty-eight Holstein cows (mean = 98 d of lactation) were assigned to a randomized block experiment in a 2 x 2 factorial arrangement of treatments. Treatments were the basal diet 1) without tallow or extract, 2) with extract but no tallow, 3) with tallow but no extract, and 4) with tallow and extract. Milk production, dry matter intake, 3.5% fat corrected milk, digestibility of neutral detergent fiber in the total tract were depressed for cows fed tallow. Addition of fermentation extract did not stimulate fiber digestion or milk production of cows fed diets with or without fat. Addition of extract did not overcome depression of fiber digestibility by cows fed tallow.
Assuntos
Aspergillus oryzae/fisiologia , Bovinos/metabolismo , Bovinos/fisiologia , Dieta/veterinária , Gorduras na Dieta/farmacologia , Digestão/fisiologia , Gorduras/farmacologia , Análise de Variância , Animais , Temperatura Corporal/efeitos dos fármacos , Temperatura Corporal/fisiologia , Peso Corporal/efeitos dos fármacos , Peso Corporal/fisiologia , Dieta/normas , Fibras na Dieta/metabolismo , Digestão/efeitos dos fármacos , Feminino , Fermentação , Temperatura Alta , Lactação , Leite/metabolismo , Distribuição AleatóriaRESUMO
The objectives of this experiment were to determine whether ruminally protected amino acids (AA) increased milk protein when this content was depressed by the addition of whole cottonseeds in the diets of early lactation Jersey cows. Treatments were 1) a control diet, 2) a diet containing whole cottonseed, and 3) a diet containing whole cottonseed and ruminally protected lysine and methionine. Cows were assigned to treatments at a mean of 7 d postpartum and remained on the experiment for 18 wk. Dry matter intake and yields of milk, milk fat, fat-corrected milk, and energy-corrected milk were not affected by treatment. Milk fat content tended to decrease for cows fed diets containing whole cottonseed. However, the percentages of milk protein, total N, and casein N were depressed by the addition of whole cottonseed and were increased by the addition of ruminally protected AA. Plasma concentrations of methionine, but not lysine, were increased when ruminally protected AA were fed, suggesting that lysine was the most limiting.
Assuntos
Aminoácidos/administração & dosagem , Bovinos/fisiologia , Óleo de Sementes de Algodão , Lactação , Leite/química , Rúmen/metabolismo , Aminoácidos/metabolismo , Fenômenos Fisiológicos da Nutrição Animal , Animais , Feminino , Metabolismo dos Lipídeos , Lisina/administração & dosagem , Lisina/metabolismo , Metionina/administração & dosagem , Metionina/metabolismo , Proteínas do Leite/metabolismoRESUMO
Four nonlactating ruminally cannulated Holstein cows were used in a 4 x 4 Latin square experiment with 4 21-d periods to determine if the effects of dietary fat would be affected by hay particle length. Treatments consisted of two levels of tallow (0 and 5%) and two hay particle lengths (short-cut and long-cut) in a 2 x 2 factorial. Diets contained alfalfa hay, corn silage, and concentrate [1:1:2, dry matter (DM) basis] fed as a total mixed ration (TMR) once per day. Samples of the 0 and 5% tallow TMR were ground and incubated in situ in polyester bags for 24 and 48 h. Ruminal samples were taken on day 21 at 0800 h and at 2-h intervals until 1600 h. The total tract digestibilities of acid detergent fiber (ADF) and neutral detergent fiber (NDF) were not affected by tallow or by hay by tallow interactions. There was a trend for tallow to improve total tract digestibility of crude protein (CP) (70.2 vs. 74.7%). After 48 h of ruminal incubation, tallow significantly decreased the digestibilities of DM, ADF, and NDF. No hay length by tallow interactions for DM, NDF, ADF or CP digestibilities occurred after 24 or 48 h. Tallow increased concentrations of propionate and decreased concentrations of acetate and valerate and the acetate-to-propionate ratio. Total volatile fatty acids increased when tallow was added to diets with short-cut hay, which suggests that when unprotected fat is added to diets with a high level of hay, a short-cut hay length may be advantageous. This result may be due to shorter rumen retention time of feed particles, which reduces the time for fatty acids to exert antimicrobial effects. Or, it may because the increased surface area of the hay particle provides more area for microbial attachment and increased fermentation.