Is otitis media with effusion associated with oxidative stress? Evaluation of thiol/disulfide homeostasis.

BACKGROUND: We evaluated the relationship between otitis media with effusion and thiol/disulfide homeostasis using a novel marker of oxidative stress. METHODS: The study group consisted of 30 patients (mean age 8.33 ±â€¯3.30 years) with bilateral otitis media with effusion admitted to our hospital. The control group consisted of 35 (mean age 7.40 ±â€¯3.97 years) age-, sex-, and body mass index-matched healthy subjects. Thiol/disulfide homeostasis was measured using a newly developed method. RESULTS: Native and total thiol levels were lower in the study than the control group (native thiols 421.37 ±â€¯72 µmol/L vs. 464.46 ±â€¯46.42 µmol/L, p < 0.05; total thiols 468.42 ±â€¯77.89 µmol/L vs. 501.32 ±â€¯50.30 µmol/L, respectively). Disulfide levels and the disulfide/native thiol and disulfide/total thiol ratios were higher in the study group (disulfides 23.56 ±â€¯4.68 µmol/L vs. 18.43 ±â€¯4.94 µmol/L; disulfide/native thiol ratio 5.65 ±â€¯1.05 vs. 3.97 ±â€¯1.03%; disulfide/total thiol ratio 5.06 ±â€¯0.83 vs. 3.66 ±â€¯0.88%, respectively). CONCLUSION: Oxidative stress may be the major cause of the increase in oxidized thiols in patients with bilateral otitis media with effusion, however, this relationship requires further investigation.

Thiol disulphide homeostasis in patients with acute myocardial infarction (AMI).

OBJECTIVE: To investigate novel oxidative stress marker thiol disulphide homeostasis in patients with acute myocardial infarction.. METHODS: The case-control study was conducted at Yildirim Beyazit University, Ankara, Turkey, between October 26, 2015 and January 26, 2016. It comprised patients of ST elevation myocardial infarction, and healthy individuals. Troponin levels, native thiol, total thiol, and disulphide were compared among the groups. Results: Of the 128 subjects, 98(76.5%) were patients and 30(23.43%) were controls. Disulphide levels were lower in the patients compared to the controls (p<0.001).As troponin levels increased, native thiol, total thiol and disulphide levels in patients decreased (p<0.05). RESULTS: Of the 128 subjects, 98(76.5%) were patients and 30(23.43%) were controls. Disulphide levels were lower in the patients compared to the controls (p<0.001).As troponin levels increased, native thiol, total thiol and disulphide levels in patients decreased (p<0.05). CONCLUSIONS: Native thiol and total thiol levels may be used as a novel oxidative stres marker in patients with acute myocardial infarction.

Thiol/disulfide homeostasis in patients with idiopathic recurrent pregnancy loss assessed by a novel assay: Report of a preliminary study.

AIM: To evaluate the relationship between idiopathic recurrent pregnancy loss (RPL) and oxidative stress (OS) by means of thiol/disulfide homeostasis via a novel technique. METHODS: Thirty-nine pregnant women diagnosed with idiopathic RPL were compared with 50 healthy pregnant women without a history of abortion. Idiopathic RPL was defined as experiencing two or more consecutive miscarriages prior to 20 weeks of gestation with the presence of normal karyotypes of couple and/or abortus materials, negative maternal screening for anticardiolipin, anti ß 2 glycoprotein antibodies and lupus anticoagulant, normal thyroid stimulating hormone, prolactin and hemoglobin A1C levels and normal pelvic sonography and/or hysterosalpingography. A new and fully automated method was used to measure plasma native thiol, total thiol and disulfide levels, based on the reduction of dynamic disulfide bonds to functional thiol groups by sodium borohydrate. RESULTS: Women with idiopathic RPL had significantly lower plasma levels of native thiol (341.89 ± 50.0 µmol/L vs. 390.84 ± 38.5 µmol/L, P < 0.001) and total thiol (386.18 ± 51.7 µmol/L vs. 435.78 ± 42.3 µmol/L, P < 0.001). Disulfide/thiol and disulfide/total thiol ratios were significantly higher in the study group. The native thiol/total thiol ratio was significantly lower in patients with idiopathic RPL. No difference was measured in disulfide, albumin and total protein plasma levels. CONCLUSIONS: The main outcome of our study indicates a relation between idiopathic RPL and OS. More importantly, the new method used in our study proposes a promising, practical and daily applicable test for evaluating patients with idiopathic RPL.

Thiol/Disulfide Balance in Patients with Familial Hypercholesterolemia.

OBJECTIVE: Herein, we investigated the balance of thiol/disulfide, with the hypothesis that the balance between disulfides and thiols, which are natural antioxidants, might be disrupted in patients with familial hypercholesterolemia, which eventually leads to endothelial damage. METHODS: In this study, we evaluated 51 patients diagnosed with familial hypercholesterolemia and 81 healthy subjects. Blood samples were taken from the patients after a minimum of 12 hours of fasting; samples were immediately centrifuged, stored in Eppendorf tubes, and preserved at -80°C. RESULTS: This study found that thiol levels are significantly lower in patients with familial hypercholesterolemia, whereas disulfide levels are higher (independent of age, gender, and body mass index). This means that in such patients, the thiol/disulfide balance changes in favour of disulfide. CONCLUSIONS: In this study, we found that the thiol/disulfide balance in patients with familial hypercholesterolemia is disrupted in favour of disulfide.

A novel method for determining the relation between nasal polyposis and oxidative stress: the thiol/disulphide homeostasis.

CONCLUSION: It is thought that oxidative stress may be the major cause of the increase in the oxide thiol form in the study group. The relationship between oxidative stress status and dynamic thiol/disulphide in nasal polyposis now needs to be investigated. OBJECTIVES: The purpose of this study was to evaluate the relation between nasal polyposis and thiol/disulphide homeostasis, used as a marker of oxidative stress, by measuring that exchange using a novel technique. MATERIALS AND METHODS: The study group consisted of 40 patients (mean age = 46.75 ± 13.92 years) with bilateral nasal polyposis patients admitted to the hospital. The control group consisted of 31 (mean age = 43.20 ± 5.68 years) age, sex, and body mass index matched healthy subjects. Thiol/disulphide homeostasis concentrations were measured using a newly-developed method (Erel & Neselioglu). RESULTS: Native thiol and total thiol levels were lower in the study group compared to the control group (native thiol = 415.8 ± 69.1 µmol/L vs 448.7 ± 37.5 µmol/L, p < 0.05; total thiol = 449.02 ± 72.0 µmol/L vs 477.28 ± 44.5 µmol/L, p < 0.05, respectively). Disulphide level and the disulphide/native thiol and disulphide/total thiol ratios were higher in the study group compared to the control group (disulphide = 16.58 ± 5.04 µmol/L vs 14.28 ± 5.3 µmol/L, p < 0.05; disulphide/native thiol ratio = 4.07 ± 1.52% vs 3.14 ± 1.04%, p < 0.05, disulphide/total thiol ratio = 3.73 ± 1.23% vs 2.94 ± 0.92%, p < 0.05, respectively).