Evaluation of bronchoalveolar lavage fluid from donkeys using four different cytological stains: A pilot study.

Bronchoalveolar lavage fluid (BALF) cytology is used for the diagnosis of non-infectious lower airway inflammation in equids. Discrepancies have been reported in the differential cell count when different staining methods were used both in humans and horses. The objective of this study was to compare the results of BALF cytology in donkeys using four different staining methods: modified May-Grunwald Giemsa (mMGG), Diff-Quick (DQ), Toluidine blue (TB) and Perls Prussian blue (PPB). Nine healthy Amiata female donkeys were enrolled. The BAL procedure was performed as previously described and pairs of cytocentrifuged BALF slides were stained with each method. No differences between mMGG and DQ were found for macrophages, neutrophils, and eosinophils, while differences were found in mast cell count between DQ vs.TB, but not between mMGG vs. DQ or mMGG vs. TB. Finally, no differences were obtained in the differential count for hemosiderophages comparing mMGG, DQ and PPB. The mMGG appears to be an excellent stain for the identification of all possible cell types, including mast cells in the BALF of donkeys. DQ, if used alone, may lead to inappropriate identification of mast cells. These results are consistent with the literature on BALF staining methods in horses.

Early versus delayed laparoscopic cholecystectomy for acute cholecystitis.

AIM: Laparoscopic cholecystectomy, currently the gold standard treatment for cholelithiasis, has been extended to treating acute cholecystitis as well. However, operation timing remains controversial. The aim of this retrospective study was to compare our data on the timing of surgery for early and delayed laparoscopic cholecystectomy for acute cholecystitis. METHODS: From January 1, 2006 to December 31, 2010, 508 laparoscopic cholecystectomy procedures were performed, 149 of which for acute cholecystitis: 122 operations were defined as early (performed within 72 hours of symptom onset) and 27 as delayed (72 hours to 9 days from symptom onset). RESULTS: There were no statistically significant differences in operating time, conversion or complications rates between early and delayed procedures. The total length of hospital stay was longer for patients who had undergone a delayed procedure. The success rates were similar irrespective of the surgeon's level of experience. CONCLUSION: Patients operated on for acute cholelithiasis between 72 hours and up to 9 days after symptom onset may benefit similarly as from an earlier operation. Delayed laparoscopic cholecystectomy for acute cholelithiasis is a feasible and safe procedure that compares favorably with early laparoscopic cholecystectomy.

[Major burn: proposal of synthetic prehospital and hospital protocols]. / Ustione maggiore: proposte di sintetici protocolli pre-ospedalieri e ospedalieri.

The Authors describe the utility of comprehensive protocols for the treatment of major burns and the adoption of these for the initial and correct management of burns by prehospital and hospital emergency staff.

Pacing thresholds of temporary epicardial electrodes: variation with electrode type, time, and epicardial position.

We compared the variation in pacing thresholds of two widely used temporary pacing electrodes at different epicardial sites in 67 patients after coronary artery operations performed with either cardioplegia or ventricular fibrillation. In 33 patients, a bare, braided pacing wire (DW) was placed on the right ventricle and a Medtronic localized epicardial electrode (MED), on each ventricle. In the other 34 patients, the DW wire was placed on the right atrium and a MED electrode, on each atrium. Pacing thresholds were measured at the time of placement; at 1 hour, 6 hours, and 12 hours postoperatively; and daily for 4 days. The pacing thresholds (mean +/- standard error of the mean) at implantation were as follows: DW wire = 0.93 +/- 0.08 V and MED electrode = 0.63 +/- 0.1 V in the ventricles and DW = 1.28 +/- 0.18 V and MED = 0.65 +/- 0.09 V in the atria. On the fourth postoperative day, the pacing thresholds were DW = 2.08 +/- 0.21 V and MED = 1.19 +/- 0.22 V in the ventricles and DW = 2.33 +/- 0.29 V and MED = 1.04 +/- 0.09 V in the atria. The pacing thresholds of both types of wire increased significantly over time, but this deterioration was more pronounced with the braided wire both on the ventricle and on the atrium. The pacing threshold patterns were not affected by chamber side or mode of myocardial preservation. The braided ventricular wire failed to capture after 24 hours in 9 of 30 patients, whereas the localized epicardial electrode captured in all instances (p < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

[Removal of tattoos by CO2 laser and acetic acid]. / Rimozione dei tatuaggi con laser CO2 ed acido acetico.

The Authors pay attention to small tattoo removal by means of the utilization of the CO2 laser. Moreover, the Authors emphasize the drawback of double treatment which, usually, the patient suffers in tattoo removal by CO2 laser. Then, the pressure of the Authors is small sized tattoo removal in only one sitting achieving so an excellent esthetic result. Besides, the Authors, in this medical study, explains two methods for tattoo removal. In the study's results, the Authors describes the manner and the time of the two lesion recovery by the different manners of treatment. Finally, the Authors affirms the great consequence of the surgical CO2 laser, they don't fail, however, to affirm that the laser and acetic acid combination is an excellent procedure for small tattoo removal.

Occlusion of coronary vein grafts secondary to non-penetrating chest injury.

Eighteen months after graft surgery to the posterior descending, circumflex, and left anterior descending coronary arteries, it seems that blunt chest trauma caused by a car accident resulted in occlusion of the grafts.

The effect of pulsed irrigation on the incidence of heterotopic ossification after total hip arthroplasty.

Heterotopic ossification (HO) is a common complication of total hip arthroplasty (THA). Pulsed lavage is being used with increasing frequency for THA. A prospective randomized, double-blind trial was initiated to determine if pulsed lavage affected the incidence of HO. A total of 94 THAs in 91 patients were analyzed. No significant difference in the incidence of HO was found between the 2 groups. Hypertrophic osteoarthritis was found to be a significant risk factor for HO. The findings suggest that the osteogenic precursor cells thought to be involved in the pathogenesis of HO possibly are derived from within the local soft tissues in the proximity of the hip joint.