Animal origin of 13th-century uterine vellum revealed using noninvasive peptide fingerprinting.

Tissue-thin parchment made it possible to produce the first pocket Bibles: Thousands were made in the 13th century. The source of this parchment, often called "uterine vellum," has been a long-standing controversy in codicology. Use of the Latin term abortivum in many sources has led some scholars to suggest that the skin of fetal calves or sheep was used. Others have argued that it would not be possible to sustain herds if so many pocket Bibles were produced from fetal skins, arguing instead for unexpected alternatives, such as rabbit. Here, we report a simple and objective technique using standard conservation treatments to identify the animal origin of parchment. The noninvasive method is a variant on zooarchaeology by mass spectrometry (ZooMS) peptide mass fingerprinting but extracts protein from the parchment surface by using an electrostatic charge generated by gentle rubbing of a PVC eraser on the membrane surface. Using this method, we analyzed 72 pocket Bibles originating in France, England, and Italy and 293 additional parchment samples that bracket this period. We found no evidence for the use of unexpected animals; however, we did identify the use of more than one mammal species in a single manuscript, consistent with the local availability of hides. These results suggest that ultrafine vellum does not necessarily derive from the use of abortive or newborn animals with ultrathin hides, but could equally well reflect a production process that allowed the skins of maturing animals of several species to be rendered into vellum of equal quality and fineness.

The York Gospels: a 1000-year biological palimpsest.

Medieval manuscripts, carefully curated and conserved, represent not only an irreplaceable documentary record but also a remarkable reservoir of biological information. Palaeographic and codicological investigation can often locate and date these documents with remarkable precision. The York Gospels (York Minster Ms. Add. 1) is one such codex, one of only a small collection of pre-conquest Gospel books to have survived the Reformation. By extending the non-invasive triboelectric (eraser-based) sampling technique eZooMS, to include the analysis of DNA, we report a cost-effective and simple-to-use biomolecular sampling technique for parchment. We apply this combined methodology to document for the first time a rich palimpsest of biological information contained within the York Gospels, which has accumulated over the 1000-year lifespan of this cherished object that remains an active participant in the life of York Minster. These biological data provide insights into the decisions made in the selection of materials, the construction of the codex and the use history of the object.

Excavating the history of ancient veterinary practices.

People have lived alongside animals for many centuries, and it can be assumed that they also treated them when they were sick. There are some texts that describe early veterinary care, but details are lacking and medical instruments are hard to come by in archaeological sites. However, as ANNELISE BINOIS: explains, archaeological findings can provide a lot more information than might be thought, and can start to give a glimpse of the vast history of veterinary medicine.

Dental development pathology in wild artiodactyls: Two prehistoric case studies from France.

In this paper we report two unique cases of dental development anomalies observed on prehistoric faunal material from France. The first is a severely malformed first incisor from a red deer, dated to the 13th-12th millennium BC, which is interpreted as a composite odontoma, a rare pseudo-tumor of odontogenic origin. The second is a Mesolithic (9th-8th millennium BC) wild boar skull presenting an anomalous tooth row including a duplication of the upper left second premolar. Both pathologies are discussed in terms of diagnosis and etiology, and comparable archeological cases are sought. We conclude by stressing that the occurrence of these two developmental anomalies appears to have a strong spontaneous component, and that caution should be exercised when considering such defects in terms of populational significance.

A dog's life: Multiple trauma and potential abuse in a medieval dog from Guimps (Charente, France).

Rescue excavations carried out around Guimps (Charente, France) in 2011 unearthed several medieval structures, including a silo containing a single dog burial. The animal, a young adult, exhibits numerous skeletal lesions. The excellent preservation of the remains allowed us to carry out a retrospective diagnosis and to demonstrate the presence of two independent pathologies, a radius-curvus and a medial patellar dislocation. These conditions are of traumatic origin, as are the many fractures the animal also displays. The possible causes of such multiple injuries are discussed and the chronology of the lesions and their skeletal distribution are examined in light of modern data. This leads us to suggest animal abuse as a probable cause and, as almost no comparable cases were found in the bibliographical record, raise the profile of the identification of animal abuse in archeology.