Correlation between intracellular pH and lactate levels in the rat brain during potassium cyanide induced metabolism blockade: a combined 31P-1H in vivo nuclear magnetic spectroscopy study.

Female Sprague-Dawley rats were chronically implanted with nuclear magnetic resonance (NMR) surface coils tuned to both 31P and 1H NMR frequencies. Alternated 31P and 1H NMR spectra were recorded at 2.5 min intervals in waking rats or rats under pentobarbital or chloral hydrate anesthesia. After a reference period, the metabolic changes were observed following intraperitoneal injection of potassium cyanide (KCN, 5 mg/kg). Among previously observed changes typical of cellular anoxia, attention was specifically paid to the relationship between the intracellular pH values and the lactate levels. The results show a strong lactate-pH correlation in waking rats, a partial decoupling under nembutal anesthesia and a complete decoupling under chloral hydrate anesthesia.

[Biochemical modifications in a case of analbuminemia (author's transl)]. / Modifications biochimiques dans un cas d'analbuminémie

A new case of analbuminemia is described for a six month old child of Algerian origin. The serum albumin concentration was 64 mg/l and its immunochemical action was identical to that of normal albumin. The system reacted by an increase of the synthesis of globulins. For the subject, the alpha1-antitrypsin, ceruloplasmin, haptoglobin, alpha2-macroglobulin, transferrin and immunoglobulins M contents were three times higher than the standard figures. However, it was possible to show that the presence of free bilirubin independent from proteins could be detected at a concentration of 17 mumol/l.

[Biochemical study on the 1st case of analbuminemia in France]. / Etude biochimique du premier cas d'analbuminémie en France

A new case of analbuminemia was described as follows for a six month's old child of Algerian origin. The discovery of the disease was made by chance, the clinical signs were limited to small oedema. The serum albumin concentration was 64 mg/1 and its immunochemical action was identical to that of normal albumin. The system reacted by an increase of the synthesis of globulins. For the subject, the alpha1-antitrypsin, ceruleoplasmin, hatoglobin, alpha2-macroglobulin, transferrin, immunoglobulins M contents were three times higher than the standard figures. The analysis of the distribution of non esterified fatty acids ususally carried by albumin was normal. On the other hand, it was possible to show that the presence of free bilirubin independant from proteins could be detected for a concentration of 17 micronmol/l. A study of the family showed a standard repartition of albumin and globulins. The genetic origin observed in the symptoms was confirmed by the consanguinity of the parents.

Urate binding to serum albumin: lack of influence on renal clearance of uric acid.

Fractional excretion of uric acid (Curate/Ccreat X 100) was determined in 1 4-year-old analbuminemic child and an age-matched control. Fractional excretion of urate was 19% in the analbuminemic child and 24% in the control subject. These values are within the range reported for normal children of this age. We conclude from this study that urate binding to serum albumin is not an important determinant of the renal clearance of uric acid, and by inference there is no significant binding of urate to serum albumin in vivo.

[Immotile cilia syndrome]. / Syndrome d'immotilité ciliaire chez un enfant de 10 ans.

Immotile cilia syndrome was diagnosed in a 10 year-old boy presenting with Kartagener syndrome: chronic otitis, sinusitis, recurrent respiratory disease and situs inversus. Electronmicroscopic examination of biopsies of the nasal mucosa showed the lack of interne dynein arms, explaining the immotile cilia syndrome in that boy.

[A case of analbuminaemia (author's transl)]. / Un cas d'analbuminémie.

A new case of analbuminaemia is described in a 6 month old child of algerian origin. The condition was discovered fortuitously, the clinical manifestations consisting only of slight oedema. Serum albumin concentration was 64 mg/l and its immunochemical behavior identical to that of normal albumin. The body react by an increase in the synthesis of globulins. In the propositus, levels of alpha, antitrypsin, caeruloplasmin, haptoglobin, jalpha2 macroglobulin, transferrin, immunoglobulin M were more than 3 times normal. Analysis of non-esterified fatty acids normally carried by albumin was normal. By contrast, it was shown that the presence of free bilirubin not bound to proteins was detected from 17 micronmol/l. Study of the family showed a normal distribution of albumin and globulins. The genetic origin seen in previously reported cases was confirmed by the co-sanguinity of the parents.