RESUMO
Three young patients with glutaric aciduria type I (age 6-23â¯years) of different ethnic origins, treated for their metabolic disease since early childhood, presented with malignant central nervous system tumors. We recommend continuing clinical follow-up, including monitoring of neurological manifestations and neuroradiological findings, in all patients with glutaric aciduria type I beyond early childhood, especially if adherence to diet is poor or the treatment was not started neonatally.
Assuntos
Erros Inatos do Metabolismo dos Aminoácidos/genética , Encefalopatias Metabólicas/genética , Neoplasias Encefálicas/genética , Encéfalo/metabolismo , Glioblastoma/genética , Glutaril-CoA Desidrogenase/deficiência , Adulto , Erros Inatos do Metabolismo dos Aminoácidos/complicações , Erros Inatos do Metabolismo dos Aminoácidos/diagnóstico por imagem , Erros Inatos do Metabolismo dos Aminoácidos/fisiopatologia , Encéfalo/diagnóstico por imagem , Encéfalo/patologia , Encefalopatias Metabólicas/complicações , Encefalopatias Metabólicas/diagnóstico por imagem , Encefalopatias Metabólicas/fisiopatologia , Neoplasias Encefálicas/complicações , Neoplasias Encefálicas/diagnóstico por imagem , Neoplasias Encefálicas/fisiopatologia , Criança , Pré-Escolar , Feminino , Glioblastoma/complicações , Glioblastoma/diagnóstico por imagem , Glioblastoma/patologia , Glutaratos/metabolismo , Glutaril-CoA Desidrogenase/genética , Humanos , Masculino , Adulto JovemRESUMO
BACKGROUND: In recent years it has become clear that newborn screening (NBS) programmes using tandem mass spectrometry identify "patients" with "classical" inborn errors of metabolism who are asymptomatic. This observation raises issues regarding medicalization of "non-diseases," potentially unnecessary treatment and unnecessary anxiety to parents. AIMS: This study aims to identify possible markers that may assist in predicting the need for treatment of infants with "classical" organic acidaemias (OA) and urea cycle disorders (UCD) diagnosed through NBS. METHODS: Medical records of all patients with classical OA and UCD detected through the Victorian NBS programme from February 2002 to January 2014, or diagnosed clinically between 1990 and January 2002 were retrospectively reviewed. RESULTS: Neonatal presentation did not always predict the need for on-going strict treatment. Blood concentrations of amino acids and acyl-carnitines and the changes thereof in follow-up samples correlated with severity in citrullinaemia-I, possibly isovaleric acidaemia but not in argininosuccinic aciduria or propionic acidaemia. Some specific mutations correlate with "attenuated" citrullinaemia-I. Gender may affect clinical outcome in propionic acidaemia. CONCLUSIONS: Changes in blood concentration of certain metabolites (amino acids, acyl-carnitines) in the first weeks of life may be predictive of the need for treatment in some disorders but not in others. Mutation analysis may be predictive in some disorders but whether or not this should be considered as second-tier testing in NBS should be discussed separately.
Assuntos
Triagem Neonatal , Distúrbios Congênitos do Ciclo da Ureia/genética , Distúrbios Congênitos do Ciclo da Ureia/metabolismo , Biomarcadores/metabolismo , Pré-Escolar , Feminino , Seguimentos , Humanos , Incidência , Lactente , Recém-Nascido , Masculino , Mutação , Triagem Neonatal/métodos , Estudos Retrospectivos , Distúrbios Congênitos do Ciclo da Ureia/diagnóstico , Distúrbios Congênitos do Ciclo da Ureia/epidemiologia , Distúrbios Congênitos do Ciclo da Ureia/terapiaRESUMO
Propionic acidaemia (PA) results from propionyl-CoA carboxylase deficiency. During metabolic decompensation, the accumulation of propionyl-CoA causes secondary hyperammonaemia through N-acetylglutamate synthetase inactivation. Carglumic acid, a structural analogue of N-acetylglutamate, was given to patients with PA (n=3) during episodes of metabolic decompensation (n=8; age range: birth to 4years), in addition to high energy/low protein intake and carnitine. Plasma ammonia concentrations normalised within 6-19h. Carglumic acid was well tolerated with no side effects noted.
Assuntos
Glutamatos/administração & dosagem , Hiperamonemia/tratamento farmacológico , Metilmalonil-CoA Descarboxilase/genética , Acidemia Propiônica/tratamento farmacológico , Erros Inatos do Metabolismo dos Aminoácidos , Amônia/sangue , Carnitina/sangue , Pré-Escolar , Feminino , Glutamatos/efeitos adversos , Humanos , Hiperamonemia/complicações , Hiperamonemia/patologia , Lactente , Recém-Nascido , Masculino , Metilmalonil-CoA Descarboxilase/metabolismo , Acidemia Propiônica/complicações , Acidemia Propiônica/patologiaRESUMO
Cobalamin C (cblC) disease, an inborn error of vitamin B(12) metabolism, results in neurometabolic, neurochemical and neuroanatomical changes. Little is known of the long-term effects of the disorder on cognition and behaviour in children. Here, the complete neuropsychological profiles of two 12-year-old girls with cblC disease are presented. The two girls were tested longitudinally with standardized neuropsychological tests including intellectual ability, attention and memory, as well as executive, adaptive and behavioural function. The results indicate the presence of intellectual dysfunction, attention problems, and concerns with behavioural aspects of executive function. Both patients demonstrated a pattern of decreasing intellectual function over time, which may reflect a growing developmental gap in comparison with their same age peers. These impairments are in contrast to the relatively spared verbal expression and comprehension abilities, as well as strengths in sociability. The findings highlight a pattern of neuropsychological strengths and weaknesses that may distinguish cblC disease from other inborn errors of metabolism. Overt sociability such as observed in these two patients may actually mask underlying cognitive deficits because the patients appear to function at a more advanced level than that reflected by quantitative assessment of intellectual and cognitive functioning. This is of clinical and functional importance and suggests that accurate determination of cognitive, adaptive and social abilities necessitates an in-depth and broad evaluation. The presence of significant intellectual and cognitive deficits also underscores the need to document and monitor cognitive development in children with cblC disease and to consider remediative and adaptive learning strategies.
Assuntos
Erros Inatos do Metabolismo/metabolismo , Erros Inatos do Metabolismo/psicologia , Vitamina B 12/metabolismo , Adaptação Psicológica , Criança , Comportamento Infantil , Transtornos do Comportamento Infantil/etiologia , Pré-Escolar , Cognição , Transtornos Cognitivos/etiologia , Feminino , Humanos , Lactente , Erros Inatos do Metabolismo/complicações , Testes Neuropsicológicos , Comportamento SocialRESUMO
BACKGROUND: Glutaric aciduria type I (GA I) is an autosomal recessive disorder of lysine and tryptophan metabolism due to a deficiency in glutaryl-CoA dehydrogenase activity. Recent reports suggest that early diagnosis through newborn screening and initiation of preventive therapy result in improved functional outcome; however, detailed neuropsychological profiles of children with GA I are seldom reported and thus the impact of the disease on cognition, motor abilities and behaviour remains uncertain. METHOD: We present detailed neuropsychological profiles of three children who were diagnosed with GA I through newborn screening and treated from early age, and one asymptomatic patient diagnosed through cascade screening. A comprehensive battery of standardized tests was administered including measures of intellectual function, attention/memory, executive function, motor skills, speech/language, as well as behavioural and adaptive skills. RESULTS: The results reveal overall average cognitive outcomes; however, subtle, but significant, fine motor and articulation deficits were observed. The results are discussed with regard to potential links between fine motor deficits and speech impairments in children with GA I. Such difficulties can impact on the child's ability to engage in academic, leisure and daily activities. CONCLUSIONS: These findings highlight the importance of in-depth assessments of all aspects of neuropsychological function in patients with GA I and provide a basis for future neuropsychological assessment in similar groups of children. In spite of relatively preserved overall functioning, using a broad range of sensitive cognitive and motor measures facilitates the detection of subtle deficits, and allows for planning of early and adequate therapeutic interventions.
Assuntos
Erros Inatos do Metabolismo dos Aminoácidos/complicações , Erros Inatos do Metabolismo dos Aminoácidos/psicologia , Encefalopatias Metabólicas/complicações , Encefalopatias Metabólicas/psicologia , Adaptação Psicológica , Erros Inatos do Metabolismo dos Aminoácidos/fisiopatologia , Transtornos da Articulação/etiologia , Encefalopatias Metabólicas/fisiopatologia , Criança , Comportamento Infantil , Pré-Escolar , Cognição , Glutaril-CoA Desidrogenase/deficiência , Humanos , Recém-Nascido , Masculino , Destreza Motora , Transtornos das Habilidades Motoras/etiologia , Triagem Neonatal , Testes NeuropsicológicosRESUMO
Non-ketotic hyperglycinaemia (NKH) is a devastating neurometabolic disorder leading, in its classical form, to early death or severe disability and poor quality of life in survivors. Affected neonates may need ventilatory support during a short period of respiratory depression. The transient dependence on ventilation dictates urgency in decision-making regarding withdrawal of therapy. The occurrence of patients with apparent transient forms of the disease, albeit rare, adds uncertainty to the prediction of clinical outcome and dictates that the current practice of withholding or withdrawing therapy in these neonates be reviewed. Both bioethics and law take the view that treatment decisions should be based on the best interests of the patient. The medical-ethics approach is based on the principles of non-maleficence, beneficence, autonomy and justice. The law relating to withholding or withdrawing life-sustaining treatment is complex and varies between jurisdictions. Physicians treating newborns with NKH need to provide families with accurate and complete information regarding the disease and the relative probability of possible outcomes of the neonatal presentation and to explore the extent to which family members are willing to take part in the decision making process. Cultural and religious attitudes, which may potentially clash with bioethical and juridical principles, need to be considered.
Assuntos
Tomada de Decisões , Ética Médica , Hiperglicinemia não Cetótica/terapia , Neonatologia/ética , Neonatologia/legislação & jurisprudência , Humanos , Hiperglicinemia não Cetótica/diagnóstico , Recém-Nascido , Responsabilidade Legal , Cuidados para Prolongar a Vida , Suspensão de Tratamento/legislação & jurisprudênciaRESUMO
OBJECTIVE: To determine the frequency, type, and severity of cardiac involvement in pediatric patients with oxidative phosphorylation (OXPHOS) disorders. STUDY DESIGN: Retrospective review of clinical and laboratory records of all patients with definitive OXPHOS disorders diagnosed and treated at the Royal Children's Hospital in Melbourne between 1984 and 2005. RESULTS: Of a total of 89 patients (male:female ratio 1.5:1) 29 (33%) had cardiac involvement: 9 as presenting symptoms, 9 developing on follow-up, and 11 with subclinical cardiac findings. Leigh or Leigh-like syndrome and complex I and combined complex I, III, and IV deficiencies were the most common clinical and laboratory diagnoses, respectively. Clinically symptomatic patients had hypertrophic cardiomyopathy (5 patients), dilated cardiomyopathy (4 patients), combined ventricular hypertrophy and systolic dysfunction (3 patients), and left ventricular noncompaction (3 patients) at first assessment. A change in the type of cardiomyopathy was noted on follow-up in 2 patients. Conduction and rhythm abnormalities were present in 7 symptomatic patients. CONCLUSIONS: Cardiac assessment in children with OXPHOS disorders may reveal subclinical abnormalities of cardiac function. Patients who present with primary cardiac features have a poor prognosis. OXPHOS disorders should be considered in the differential diagnosis of children presenting with otherwise unexplained cardiomyopathy.
Assuntos
Cardiopatias/epidemiologia , Doenças Mitocondriais/epidemiologia , Adolescente , Idade de Início , Causalidade , Criança , Pré-Escolar , Estudos de Coortes , Comorbidade , Feminino , Seguimentos , Humanos , Lactente , Recém-Nascido , Masculino , Doenças Mitocondriais/classificação , Prevalência , Estudos Retrospectivos , Distribuição por Sexo , Análise de Sobrevida , Vitória/epidemiologiaRESUMO
Inhibition of protein kinase C [PK-C] activity by sphingosine and its derivatives has been suggested to play a role in the pathogenesis of sphingolipidoses. In the present study, PK-C activity and PK-C-mediated phosphorylation of endogenous substrates were studied in skin fibroblasts from patients with mucolipidosis type 4 [ML-4], in which there is accumulation of the phospholipids phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine as well as gangliosides. Cytosolic PK-C activity in 5 ML-4 cell lines was comparable to that in control cells. PK-C activity in the particulate fraction of these cells was 84 +/- 14 pmol 32P/mg protein per min compared with 267 +/- 26 in control cells. Increasing the concentrations of the activating lipids in the reaction mixture did not enhance PK-C activity in ML-4 cells, suggesting a non-competitive inhibition of the kinase. Following partial purification of the enzyme from the particulate fraction PK-C activity increased to 288 +/- 14 and 339 +/- 12 pmol 32P/mg protein per min in ML-4 and control cells, respectively. The phosphorylation pattern of endogenous substrates in the particulate fraction of ML-4 cells differed from that in control cells both in the absence and in the presence of calcium and activating lipids. We suggest that PK-C may be involved in the pathogenesis of sphingolipidoses and that this may represent an example for a new type of pathogenetic mechanisms in inborn errors of metabolism.
Assuntos
Mucolipidoses/enzimologia , Proteína Quinase C/antagonistas & inibidores , Humanos , Pessoa de Meia-Idade , Modelos Biológicos , Mucolipidoses/metabolismo , Fosforilação , Esfingolipidoses/etiologiaRESUMO
We studied the effect of fatty acids and their acyl-CoA esters on protein kinase C (PK-C) activity in human skin fibroblasts. Butyrate, octanoate, palmitate and oleate did not alter PK-C activity in either cytosolic or particulate fraction. In the presence of calcium, phosphatidylserine and diacylglycerol, both palmitoyl-CoA (Pal-CoA) and oleoyl-CoA (Ole-CoA) enhanced particulate PK-C activity by approx. 70% and octanoyl-CoA (Oct-CoA) by approx. 35%. Partially purified cytosolic PK-C activity was enhanced by 60-70% by 13.5 microM of either Pal-CoA or Ole-CoA. Basal histone phosphorylation (i.e., PK-C-independent phosphorylation) was decreased in the particulate fraction in the presence of these esters in a concentration-dependent manner. Both Pal-CoA and Ole-CoA fully substituted diacylglycerol in activating the kinase in both the cytosolic and particulate fractions, whereas Oct-CoA had a moderate effect. The pattern of endogenous cytosolic and particulate protein phosphorylation was altered in the presence of either Pal-CoA or Ole-CoA. We conclude that long-chain fatty acyl-CoA esters may activate PK-C in non-stimulated fibroblasts, i.e., in the absence of physiological diacylglycerol formation. Activation of PK-C in stimulated fibroblasts, i.e., in the presence of an elevated diacylglycerol concentration, is less pronounced. These results support the hypothesis that activation of PK-C and alteration of endogenous protein phosphorylation may play a role in the pathogenesis of diseases in which there is intracellular accumulation of fatty acyl-CoA esters, such as in inborn fatty-acid oxidation defects.
Assuntos
Acil Coenzima A/farmacologia , Ácidos Graxos não Esterificados/farmacologia , Fosfoproteínas/metabolismo , Proteína Quinase C/metabolismo , Pele/enzimologia , Análise de Variância , Cálcio/farmacologia , Células Cultivadas , Citosol/enzimologia , Diglicerídeos/farmacologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/enzimologia , Humanos , Cinética , Fosfatidilserinas/farmacologia , Fosforilação , Proteína Quinase C/isolamento & purificação , Relação Estrutura-AtividadeRESUMO
Calcium-activated, phospholipid-dependent protein kinase (protein kinase C) has been implicated in the regulation of transport processes in a variety of tissues and cell lines. To establish whether protein kinase C participates in the regulation of renal phosphate transport, we examined the effect of phorbol myristate acetate (PMA), a potent activator of protein kinase C, on phosphate uptake in fresh preparations of mouse renal tubules, and we correlated the changes in transport activity with protein kinase C activation and phosphorylation of endogenous proteins. PMA inhibited Na+-dependent phosphate transport, elicited a rapid translocation of protein kinase C from the cytosolic to the particulate fraction and stimulated the phosphorylation of endogenous substrates in the cytosolic and brush border membrane fractions. Effects of PMA were maximal after a 10 min incubation of the tubules with the activator. 4 alpha-Phorbol, an inert analogue of PMA, did not elicit any of these effects. The present results demonstrate a temporal correlation between inhibition of Na+-dependent phosphate transport, translocation and activation of protein kinase C, and phosphorylation of endogenous proteins in mouse renal tubules. These data suggest that protein kinase C may play a regulatory role in phosphate transport in mammalian kidney.
Assuntos
Túbulos Renais/enzimologia , Fosfatos/metabolismo , Fosfoproteínas/metabolismo , Proteína Quinase C/metabolismo , Acetato de Tetradecanoilforbol/farmacologia , Animais , Transporte Biológico/efeitos dos fármacos , Citosol/enzimologia , Citosol/metabolismo , Ativação Enzimática/efeitos dos fármacos , Córtex Renal , Túbulos Renais/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Microvilosidades/enzimologia , Microvilosidades/metabolismo , FosforilaçãoRESUMO
Although calcium-activated, phospholipid-dependent protein kinase (protein kinase C) has been implicated in the regulation of various steroidogenic pathways, comparatively little is known of its role in the metabolism of vitamin D. The present study was undertaken to determine whether protein kinase C is involved in the regulation of renal mitochondrial 25-hydroxyvitamin D3-24-hydroxylase (24-hydroxylase), the first enzyme in the C-24 oxidation pathway, a major catabolic pathway for vitamin D metabolites in kidney and other target tissues. We examined the effect of phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, on 24-hydroxylase activity in fresh mouse renal tubules and correlated the changes in 24,25-dihydroxyvitamin D3 [24,25-(OH)2D3] production with translocation of protein kinase C and phosphorylation of mitochondrial proteins. PMA stimulated 24,25-(OH)2D3 synthesis, protein kinase C translocation from the cytosolic to the mitochondrial fraction, and phosphorylation of 30-35 K, 40 K, and 50 K mitochondrial proteins derived from 32P-labeled tubules. 4 alpha-Phorbol 12,13 didecanoate, an insert analog of PMA, did not elicit any of these effects. The synthetic diacylglycerol, oleoylacetyl glycerol, also stimulated 24,25-(OH)2D3 synthesis, whereas the protein kinase C inhibitors, H-7 and staurosporine, inhibited 24-hydroxylase activity. PMA did not further stimulate 24,25-(OH)2D3 production in tubules derived from mutant (Hyp) mice in which 24-hydroxylase and protein kinase C activities are elevated relative to normal. However, after treatment with H-7, 24-hydroxylase activity was reduced in both strains, and genotype differences were no longer apparent. Finally, H-7 failed to inhibit the induced renal 24-hydroxylase in tubules isolated from 1,25-dihydroxyvitamin D3-treated mice. These findings suggest a role for protein kinase C in the regulation of constitutive renal 24-hydroxylase and implicate the kinase in the aberrant expression of the hydroxylase in the Hyp mouse.
Assuntos
Sistema Enzimático do Citocromo P-450 , Córtex Renal/enzimologia , Túbulos Renais/enzimologia , Proteína Quinase C/metabolismo , 1-(5-Isoquinolinasulfonil)-2-Metilpiperazina , 25-Hidroxivitamina D3 1-alfa-Hidroxilase , Animais , Calcifediol/metabolismo , Calcitriol/farmacologia , AMP Cíclico/metabolismo , Citosol/enzimologia , Diglicerídeos/farmacologia , Indução Enzimática , Homeostase , Isoquinolinas/farmacologia , Cinética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias/enzimologia , Fosforilação , Piperazinas/farmacologia , Esteroide Hidroxilases/biossíntese , Esteroide Hidroxilases/metabolismo , Acetato de Tetradecanoilforbol/farmacologia , Vitamina D3 24-HidroxilaseRESUMO
We report the successful use of raw cornstarch to maintain normoglycemia in two children with nesidioblastosis. Despite subtotal distal pancreatectomy both children required frequent daytime feedings and continuous nocturnal intragastric polycose administration to prevent symptomatic hypoglycemia. Raw cornstarch administration consistently maintained blood glucose levels in the normal range. Both children still need frequent daily feedings but no hypoglycemic episodes are reported. They have normal development and have maintained satisfactory growth during the 22 mo since initiation of treatment.
Assuntos
Pancreatopatias/tratamento farmacológico , Amido/uso terapêutico , Glicemia/metabolismo , Criança , Pré-Escolar , Glucanos/uso terapêutico , Humanos , Insulina/sangue , Pancreatectomia , Pancreatopatias/sangue , Pancreatopatias/cirurgiaRESUMO
BACKGROUND: Respiratory chain (RC) disorders are clinically, biochemically, and molecularly heterogeneous. The lack of standardized diagnostic criteria poses difficulties in evaluating diagnostic methodologies. OBJECTIVE: To assess proposed adult RC diagnostic criteria that classify patients into "definite," "probable," or "possible" categories. METHODS: The authors applied the adult RC diagnostic criteria retrospectively to 146 consecutive children referred for investigation of a suspected RC disorder. Data were collected from hospital, genetics, and laboratory records, and the diagnoses predicted by the adult criteria were compared with the previously assigned assessments. RESULTS: The authors identified three major difficulties in applying the adult criteria:lack of pediatric-specific criteria; difficulty in segregating continuous data into circumscribed major and minor criteria; and lack of additivity of clinical features or enzyme tests. They therefore modified the adult criteria to allow for pediatric clinical and histologic features and for more sensitive coding of RC enzyme and functional studies. Reanalysis of the patients' data resulted in congruence between the diagnostic certainty previously assigned by the authors' center and that defined by the new general RC diagnostic criteria in 99% of patients. CONCLUSIONS: These general diagnostic criteria appear to improve the sensitivity of the adult criteria. They need further assessment in prospective clinical and epidemiologic studies.
Assuntos
Transporte de Elétrons , Doenças Mitocondriais/metabolismo , Doenças Mitocondriais/patologia , Adulto , Biópsia , Células Cultivadas , Criança , DNA Mitocondrial/genética , Esclerose Cerebral Difusa de Schilder/genética , Esclerose Cerebral Difusa de Schilder/metabolismo , Esclerose Cerebral Difusa de Schilder/patologia , Fibroblastos/citologia , Humanos , Doença de Leigh/genética , Doença de Leigh/metabolismo , Doença de Leigh/patologia , Síndrome MELAS/genética , Síndrome MELAS/metabolismo , Síndrome MELAS/patologia , Doenças Mitocondriais/genética , Fibras Musculares Esqueléticas/enzimologia , Fibras Musculares Esqueléticas/patologia , Músculo Esquelético/metabolismo , Músculo Esquelético/patologia , Mutação , Prostaglandina-Endoperóxido Sintases/metabolismo , Sensibilidade e EspecificidadeRESUMO
We measured the production of two eicosanoids, prostaglandin E2 and thromboxane-B2, by rat glial cell cultures under basal conditions, following stimulation with phorbol-12-myristate-13-acetate and the bacterial endotoxin lipopolysaccharide, and following treatment with synthetic glucocorticoids. Stimulation of rat glial cells in culture with either phorbol-12-myristate-13-acetate or lipopolysaccharide caused a 1.5-5.0-fold increase in prostaglandin E2 production, but did not affect thromboxane production. Pretreatment of the cultures with dexamethasone markedly inhibited the stimulated production of prostaglandin E2 but had only a modest effect on basal production. Dexamethasone did not affect the activity of the enzyme protein kinase C, a putative regulator of eicosanoid synthesis. Our findings show that glucocorticoids have the potential to modulate central nervous system eicosanoid production particularly under conditions of stimulated production, such as inflammatory and demyelinating disorders. This mechanism may explain, at least in part, the therapeutic benefit of glucocorticoids in patients with multiple sclerosis.
Assuntos
Dinoprostona/biossíntese , Glucocorticoides/fisiologia , Neuroglia/metabolismo , Tromboxano B2/biossíntese , Animais , Dexametasona/farmacologia , Lipopolissacarídeos/farmacologia , Neuroglia/citologia , Fosfolipases A/metabolismo , Proteína Quinase C/metabolismo , Ratos , Acetato de Tetradecanoilforbol/farmacologiaRESUMO
We describe a female infant who presented with severe intracranial bleeding and was found to have a hereditary deficiency of vitamin K-dependent coagulation factors. She also had mild stippling of the left femoral epiphysis and shortness of the distal phalanges of the fingers. We studied the possible relationship between these abnormalities and a peroxisomal defect and followed their responses to treatment with vitamin K. The level of vitamin K-dependent clotting factors returned to near-normal following treatment with pharmacological doses of vitamin K, but there was no effect on the skeletal abnormalities.
Assuntos
Transtornos da Coagulação Sanguínea/genética , Osso e Ossos/anormalidades , Erros Inatos do Metabolismo , Deficiência de Vitamina K/genética , Feminino , Humanos , LactenteRESUMO
Two forms of X-linked hypophosphatemia occur in the mouse. One form, caused by the Hyp gene, is a counterpart of human X-linked hypophosphatemic "vitamin D-resistant rickets". The other, recently characterized, is caused by a different gene (Gy) closely linked to Hyp on the mouse X-chromosome. The Gy mutation also impairs cochlear function in the mouse. We measured hearing in 22 patients with X-linked hypophosphatemia; five, including 2 mother-son pairs, had sensorineural hearing deficits due to cochlear dysfunction. We suggest the disease in these persons may be the human counterpart of the Gy phenotype in the mouse, which implies there are 2 forms of X-linked hypophosphatemia in humans.
Assuntos
Ligação Genética , Perda Auditiva Neurossensorial/genética , Hipofosfatemia Familiar/genética , Cromossomo X , Adulto , Animais , Audiometria de Tons Puros , Cóclea/fisiopatologia , Feminino , Perda Auditiva Neurossensorial/complicações , Perda Auditiva Neurossensorial/fisiopatologia , Humanos , Hipofosfatemia Familiar/complicações , Doenças do Labirinto/genética , Doenças do Labirinto/fisiopatologia , Masculino , Camundongos , Pessoa de Meia-IdadeRESUMO
Molybdenum cofactor deficiency is a rare inborn error of metabolism with generally severe symptoms, most often including neonatal seizures and severe developmental delay. We describe a patient with an unusually mild form of the disease. Two mutations in MOCS2A (molybdenum cofactor synthesis enzyme 2A) were identified: a single base change, 16C > T, that predicts a Q6X substitution on one allele and a 19G > T transversion that predicts a valine to phenylalanine substitution, V7F, on the second. It is postulated that the milder clinical symptoms result from a low level of residual molybdopterin synthase activity derived from the 19G > T allele.
Assuntos
Coenzimas , Metaloproteínas/deficiência , Mutação , Sulfurtransferases/genética , Alelos , Sequência de Bases , Encéfalo/patologia , Pré-Escolar , Análise Mutacional de DNA , DNA Complementar/metabolismo , Éxons , Feminino , Glutamina/química , Heterozigoto , Humanos , Íntrons , Imageamento por Ressonância Magnética , Modelos Químicos , Dados de Sequência Molecular , Cofatores de Molibdênio , Fenilalanina/química , PteridinasRESUMO
Holocarboxylase synthetase (HLCS) deficiency (HLCSD) is a rare autosomal recessive disorder of biotin metabolism. HLCS catalyzes the biotinylation of the four human biotin-dependent carboxylases. Using the newly available human genomic sequence, we report the map of HLCS genomic structure and the predicted exon/intron boundaries. Moreover, the molecular studies of four patients (two Italians, one Iranian, and one Australian) affected by HLCS deficiency are here reported. The clinical findings, the age of onset, and response to biotin treatment differed between our patients. The diagnosis was made by organic acid analysis and confirmed by enzymatic analysis in three patients. Six mutations in the HLCS gene were identified, including two novel (N511K and G582R) and four known missense mutations (L216R, R508W, V550M, and G581S). Five of the mutations are localized within the HLCS biotin-binding domain, whereas the L216R amino acid change is located in the N-terminal region outside of the putative biotin-binding domain. This mutation, previously reported in a heterozygous state, was detected for the first time in a patient with homozygous status. The patient's severe clinical phenotype and partial responsiveness to biotin support a genotype-phenotype correlation through the involvement of residues of the N-terminal region in a substrate specificity recognition or regulation of the HLCS enzyme.
Assuntos
Carbono-Nitrogênio Ligases/genética , Deficiência de Holocarboxilase Sintetase/genética , Acidose/enzimologia , Acidose/genética , Ácidos/urina , Idade de Início , Substituição de Aminoácidos , Sítios de Ligação , Biotina/uso terapêutico , Biotinilação , Células Cultivadas , Análise Mutacional de DNA , DNA Complementar/genética , Éxons/genética , Evolução Fatal , Genes , Genes Recessivos , Genótipo , Deficiência de Holocarboxilase Sintetase/sangue , Deficiência de Holocarboxilase Sintetase/tratamento farmacológico , Deficiência de Holocarboxilase Sintetase/patologia , Humanos , Lactente , Deficiência Intelectual/enzimologia , Deficiência Intelectual/genética , Íntrons/genética , Masculino , Mutação de Sentido Incorreto , Fenótipo , Estrutura Terciária de Proteína , Mapeamento por Restrição , Pele/patologia , Especificidade por SubstratoRESUMO
A 16-month old baby developed severe respiratory failure because of acute laryngitis and required mechanical ventilation. Intubation was complicated by aspiration and development of chemical pneumonia. Following 4 days of treatment the child was successfully extubated. Thirty six hours after extubation the patient again developed respiratory failure and on chest X-ray pneumomediastinum was seen and later evidence of a mediastinal abscess. Conservative treatment, with antibiotics, effected complete cure.
Assuntos
Abscesso/etiologia , Mediastinite/etiologia , Infecções Pneumocócicas , Feminino , Humanos , Lactente , Enfisema Mediastínico/etiologia , Mediastinite/complicações , Insuficiência Respiratória/etiologiaRESUMO
The effect of sphingosine on lipopolysaccharide (LPS)-mediated protein kinase C (PKC) activation and prostaglandin E2 (PGE2) and nitric oxide (NO) production was studied in primary cultures of rat glial cells. Incubation of cells with LPS elicited translocation of PKC from the cytosolic to the membranous compartment, as shown by measuring PKC activity and by immunoblotting. Under these conditions, a sustained increase in both PGE2 and NO production was measured. Thus, PGE2 levels were 259 +/- 28 (n = 8) and 230 +/- 48 (n = 4) (control levels 11.4 +/- 5.2 (n = 5) and 13 +/- 7.5 (n = 3)) pg/ml, at 24 and 48 h, respectively. NO levels were 9.3 +/- 0.9 (n = 10) and 11.6 +/- 0.8 (n = 9) (control levels 0.4 +/- 0.18 and 1.0 +/- 0.44) nmol/ml, at 24 and 48 h, respectively. Sphingosine, a naturally occurring compound, which inhibits PKC activity, elicited a concentration-dependent decrease in LPS-mediated PGE2 production. This inhibition was more pronounced after 48 h than after 24 h of incubation (IC50 = 8 and 20 microg sphingosine, respectively). By contrast, sphingosine did not inhibit NO production under the same conditions. We conclude that sphingosine may be involved in modulation of the local inflammatory response in glial cells, at least in part. We also surmise that LPS-mediated PGE2 production and NO production are probably regulated by different mechanisms, i.e., a PKC-dependent and a PKC-independent mechanism.