Experimental efficacy of a vaccine against Rhipicephalus australis.

Despite several decades of chemical use the cattle tick, Rhipicephalus australis, still represents an important threat for breeders of tick susceptible cattle under tropical conditions. The intense use of acaricides has lead to the development of multi-resistant strains of ticks and alternative means of control need to be developed to maintain control. A vaccine against ticks, based on the Boophilus microplus Bm86 protein, is one of the promising alternative means of tick control. In this investigation, an experimental vaccine was developed based on the orthologous R. australis Bm86 sequence identified from local R. australis strains and a recombinant protein expressed in Escherichia coli. The efficacy of this vaccine against a local strain of R. australis was assessed under controlled conditions. Vaccinated cattle presented 40.3% fewer ticks than unvaccinated cattle and tick reproductive performance was also affected by the vaccine. Ticks that engorged on vaccinated animals laid 51.2% less egg mass and tick fertility decreased by 18.8% compared to the control group. The use of the vaccine reduced the tick population by 74.2% at each generation. The tick vaccine can be used in an integrated tick control program.

Evaluation of three adjuvants with respect to both adverse effects and the efficacy of antibody production to the Bm86 protein.

Cattle tick infestations remain an important burden for farmers in tropical area like in New Caledonia. With the development of acaricide resistance, tick vaccines should be an attractive alternative to control ticks but their efficacy needs to be improved. In this study three adjuvants were studied in an experimental tick vaccine with a Bm86 protein to assess their performance in terms of antibody productions and adverse reactions following vaccinations. The water-in-oil adjuvant ISA 61 VG led to higher antibody titers compared to a water-in-oil-in-water adjuvant ISA 201 VG and an aqueous polymeric adjuvant Montanide Gel 01. Vaccinations with these three adjuvants did not produce severe general reaction but an increase in skin thickness was observed especially with both oil-based emulsions. These results indicated that the water-in-oil adjuvant is the most interesting to use for this vaccine but local adverse reactions remain an issue.