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This study aimed to investigate the impact of bar velocity during the eccentric phase on subsequent concentric performance in the young and elderly. Twelve elderly women (65.2+4.2 years) and 16 young women (23.1+2.1 years) were randomly assigned to four experimental conditions that combined high-velocity eccentric actions (HVECC; maximum velocity) and moderate-velocity eccentric actions (MVECC; 2.0 s), using 30% and 60% of their 1-repetition maximum (1RM) on the Smith machine bench press. Measurements of mean propulsive velocity (MPV), peak velocity (PV), and mean power (MP) were recorded. Two-way ANOVA with repeated measures was applied for each variable, when needed, the Bonferroni post hoc was used. Statistical significance was set at p<0.05, and effect size (ES) was established by Cohen's d z. The results demonstrated that elderly women showed improved performance in MPV (+10.0%; ES=- 0.58; p=0.009) and MP (+11.7%; ES=- 0.14; p=0.045) when utilizing HVECC at 30% 1RM only. Moreover, young women exhibited superior performance for both 30% and 60% 1RM when using HVECC for all variables analyzed (p<0.05). The HVECC appears to have an impact on the subsequent performance of the concentric phase, particularly when lighter loads (30% 1RM) are utilized, regardless of the age group.
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Força Muscular , Levantamento de Peso , Humanos , Feminino , Idoso , Levantamento de Peso/fisiologia , Adulto Jovem , Força Muscular/fisiologia , Fatores Etários , Treinamento Resistido/métodos , Pessoa de Meia-Idade , Adulto , Músculo Esquelético/fisiologia , Desempenho Atlético/fisiologiaRESUMO
ABSTRACT: Cornejo-Daza, PJ, Sánchez-Valdepeñas, J, Páez-Maldonado, J, Rodiles-Guerrero, L, Boullosa, D, León-Prados, JA, Wernbom, M, and Pareja-Blanco, F. Acute responses to traditional and cluster-set squat training with and without blood flow restriction. J Strength Cond Res 38(8): 1401-1412, 2024-To compare the acute responses to different set configurations (cluster [CLU] vs. traditional [TRA]) under distinct blood flow conditions (free vs. restricted) in full-squat (SQ). Twenty resistance-trained males performed 4 protocols that differed in the set configuration (TRA: continuous repetitions; vs. CLU: 30 seconds of rest every 2 repetitions) and in the blood flow condition (FF: free-flow; vs. blood flow restriction [BFR]: 50% of arterial occlusion pressure). The relative intensity (60% 1RM), volume (3 sets of 8 repetitions), and resting time (2 minutes) were equated. Mean propulsive force (MPF), velocity (MPV) and power (MPP), and electromyography (EMG) parameters were recorded during each repetition. Tensiomyography (TMG), blood lactate, countermovement jump (CMJ) height, maximal voluntary isometric contraction, in SQ, and movement velocity against the load that elicited a 1 m·s -1 velocity at baseline (V1-load) in SQ were assessed at pre- and post-exercise. The CLU protocols allowed a better maintenance of MPF, MPV, MPP, and EMG median frequency during the exercise compared to TRA (clu-time interaction, p < 0.05). The TRA protocols experienced greater impairments post-exercise in TMG- and EMG-derived variables (clu-time interaction, p < 0.05) and SQ and CMJ performance (clu-time interaction, p = 0.08 and p < 0.05, respectively), as well as higher blood lactate concentrations (clu-time interaction, p < 0.001) than CLU. Moreover, BFR displayed decreases in TMG variables (bfr-time interaction, p < 0.01), but BFR-CLU resulted in the greatest reduction in twitch contraction time ( p < 0.001). Cluster sets reduced fatigue during and after the training session and BFR exacerbated impairments in muscle mechanical properties; however, the combination of both could improve contraction speed after exercise.
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Eletromiografia , Força Muscular , Músculo Esquelético , Treinamento Resistido , Humanos , Masculino , Treinamento Resistido/métodos , Adulto Jovem , Músculo Esquelético/fisiologia , Músculo Esquelético/irrigação sanguínea , Força Muscular/fisiologia , Adulto , Fluxo Sanguíneo Regional/fisiologia , Ácido Láctico/sangue , Levantamento de Peso/fisiologiaRESUMO
The objectives of this study were to evaluate the acute effects of the sequence order of drop jumps (DJ) and dynamic stretching (DS) on sprinting performances in competitive athletes and to investigate the relationships between post-activation performance enhancement (PAPE) in sprint performance and lower limb power. Thirteen male jumpers and sprinters participated in this study (19 ± 2 years; 177 ± 7 cm; 71.7 ± 5.6 kg). Through a randomized crossover design, the athletes were exposed to three different conditions after a standardized warm-up: DS+DJ, DJ+DS, and control. Sprinting performance over 40 m was analysed with consideration of initial (0 to 20 m) and final acceleration (20 to 40 m) phases. The effect of intervention was examined by two-way repeated-measures of ANOVA. Pearson's correlation test was used to determine the association between PAPE during sprinting and jump performance. There was no effect of any factor on 40-m sprint performance. Meanwhile, the performance at 20-40 m was higher after the DS+DJ condition when compared to baseline (8.79 ± 0.43 vs. 8.91 ± 0.35 m/s; p = 0.015). However, the initial acceleration was worsened in the DJ+DS condition when compared to baseline (6.26 ± 0.25 vs. 6.22 ± 0.26 m/s; p = 0.002). There was a negative correlation between CMJ height and the improvement in final acceleration (r = -0.741; p = 0.004). The use of DS prior to DJ is an effective strategy to improve performance in the final acceleration phase (20-40 m). The athletes with lower levels of lower limb power benefited the most from this PAPE strategy.
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PURPOSE: The aim of the present study was to analyze the effect of different eccentric tempos on eccentric kinetics and kinematics and the subsequent concentric performance when performing countermovement jumps against different loads. METHODS: After 1-repetition-maximum assessment and 2 familiarization sessions, 13 well-trained participants performed, in randomized order, 12 sets (4 tempos × 3 loads) of 4 repetitions of the loaded countermovement-jump exercise. The eccentric tempos analyzed were 5 and 2 seconds, as fast as possible, and accelerated (ie, without pause between repetitions), while the loads used were 30%, 50%, and 70% of 1-repetition maximum. Several kinetic and kinematic variables during both phases were recorded by linking a linear position transducer to the barbell. RESULTS: The eccentric work was greater in the accelerated condition despite no changes in the eccentric depth. The peak and mean propulsive velocities were greater in the as-fast-as-possible and accelerated conditions. Correlation analysis showed that, compared with the 5-second condition, the increased concentric performance in the accelerated condition was related to the difference in eccentric work performed in the last 100 milliseconds of the eccentric phase (r > .770). CONCLUSIONS: Contrary to current practices, the current study highlights the need for performing the eccentric phase of loaded countermovement jumps, a common exercise performed by athletes for both training and evaluation purposes, as fast as possible. This allows not only a greater eccentric work but also improved concentric performance.
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Atletas , Músculo Esquelético , Humanos , Exercício Físico , Fenômenos Biomecânicos , Força MuscularRESUMO
The aim of this study was to verify post-activation performance enhancement (PAPE) in jumping and sprinting after two endurance volume-equated running protocols with different intensities, in runners vs. active individuals. Nine recreational runners (age: 34.5 ± 9.3 years, body mass: 73.1 ± 11.9 kg, body height: 1.76 ± 0.06 m, 17.4 ± 4.4 %body fat; maximum aerobic speed [MAS]: 16.4 ± 1.0 kmâ¢h-1), and 9 active individuals (age: 34.1 ± 9.4 years; body mass: 83.2 ± 7.7 kg; body height: 1.79 ± 0.06 m; 25.6 ± 5.4 %body fat; MAS: 13.3 ± 1.2 kmâ¢h-1) volunteered for participation. The evaluations were performed over three days as follows: 1) anthropometric measures, physical fitness tests, and the University of Montreal Track Test (UMTT) to determine MAS and the distance to be covered in the running protocols; 2 and 3) the countermovement jump (CMJ) and the flying 20-m sprint (SPRINT) were assessed pre- and post-running at 70% of MAS or a time trial race (TTR), equated by volume and completed in random order. A three-way ANOVA (time*group*running) was performed to analyze the PAPE effects. The results showed a time effect (F = 10 .716; p < 0.01) and a group*running interaction (F = 12.094; p < 0.01) for the CMJ, indicating that active individuals demonstrated PAPE after running at 70% of MAS, while for runners both running interventions (70% of MAS and TTR) induced PAPE in CMJ performances. For the SPRINT, a time*group interaction (F = 4.790; p = 0.044) and a group effect were observed, with runners showing greater SPRINT performances than active individuals. From the current results, it can be suggested that training background and intensity can modulate PAPE responses in jumping and sprinting after volume-equated running protocols at different intensities.
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Purpose: This study examined how a low dose of an eccentric-oriented lunge exercise could induce the repeated-bout effect (RBE) and affect the subsequent post-activation performance enhancement (PAPE) in recreational runners. Methods: Twenty male recreational runners (32.1 ± 2.8 years; 173.4 ± 6.1 cm; 73.3 ± 11.5 kg; 57.8 ± 7.2 mL·kg-1·min-1) were divided into control (N = 10) and experimental (N = 10) groups. In the first and fourth weeks, the groups were assessed for jump capacity, dynamic balance, and submaximal running kinematics before and after an incremental shuttle-run test until exhaustion. The experimental group was also submitted to two sessions of the eccentric-oriented lunge exercise (3 sets of 10 repetitions with 2 min of passive recovery) in the second and third weeks. Results: We observed that the first session promoted muscle damage, which was significantly (p < .05) reduced after the second training session, thus indicating an RBE. Meanwhile, there was no effect of the RBE on dynamic balance and submaximal running kinematics in the post-intervention. However, there was a significant increase in countermovement jump height (p = .008) for the experimental group when compared to the control group, although no PAPE was observed. Conclusions: The current results demonstrate that a simple, low-dose eccentric-oriented exercise may induce an RBE, leading to reduced muscle damage and a possibly improved lower limbs' muscle power in recreational runners. However, the absence of PAPE effects suggests that the RBE may not directly influence the potentiation/fatigue balance after fatiguing running exercises.
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We investigated the influence of anaerobic speed reserve (ASR) on post-activation performance enhancement (PAPE). Twenty-two endurance runners and triathletes were evaluated for maximum sprinting speed (MSS) and countermovement jump (CMJ) before (non-fatigued) and after (fatigued) an incremental running test. They were allocated in LASR (low-ASR) and HASR (high-ASR) groups for comparisons between conditions. HASR showed greater CMJ and MSS (both p ≤ 0.005) performances, with enhanced CMJ in fatigued condition (p ≤ 0.008). Significant correlations were found between ASR, CMJ, and MSS in both conditions (p ≤ 0.01) for the entire sample, and between ∆CMJ and ∆MSS (p ≤ 0.001) in LASR. Our results show that ASR profile influences PAPE.
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PURPOSE: Postactivation performance enhancement (PAPE), which refers to the phenomena associated with the attainment of enhanced performance in sport-specific tasks after a conditioning activity, is an important objective of warming-up practices in many sports. This is even more relevant for sprinters, as potential increases in sprinting speed will directly influence their competitive results. This systematic review with meta-analysis evaluated the effects of different PAPE protocols (ie, using plyometrics, strength-power exercises, and resisted/assisted sprints) on the sprinting performance (ie, sprint time or sprint speed) of competitive sprinters. METHODS: Initially, 1205 records published until last December 18 were identified, using the following databases: PubMed/MEDLINE, Scopus, and Clarivate Web of Science. After removing duplicates and screening titles and abstracts, 14 high-quality studies met the inclusion criteria for the meta-analysis. RESULTS: Overall, there were no significant changes in sprint performance after implementing various types of conditioning activities (standardized mean difference [SMD] = 0.16 [95% CI, -0.02 to 0.33]; Z = 1.78; P = .08; I2 = 0%). In addition, when comparing prechanges and postchanges between experimental, control, and other conditions, no significant differences were found in sprint speed or time across all studies (SMD = 0.09 [95% CI, -0.10 to 0.28]; Z = 0.92; P = .36; I = 0%). CONCLUSIONS: Results revealed that different types of conditioning activities may not be capable of acutely enhancing the sprint speed of competitive sprinters. This aligns with previous observations indicating that sprinting is a highly stable physical capacity, a phenomenon that is even more consistent among elite sprinters. Coaches and sport scientists should collaborate to develop more efficient PAPE protocols for these highly specialized athletes, with special attention to study design and individualization, while considering their effects on acceleration versus top speed.
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Desempenho Atlético , Corrida , Humanos , Desempenho Atlético/fisiologia , Corrida/fisiologia , Condicionamento Físico Humano/métodos , Exercício de Aquecimento/fisiologia , Exercício Pliométrico , Treinamento Resistido/métodos , Comportamento Competitivo/fisiologiaRESUMO
The 2-minutes walking test (2-MWT) is a valid and reliable test that has a high correlation with the distance walked in the 6-minutes walking test (6-MWT). However, to date, no study has determined the relationship between 2-MWT performance and the aerobic fitness indices obtained during a maximal incremental test to confirm if this test is a valid surrogate of aerobic fitness in apparently healthy older adults. The main objective of this work was to identify the factors associated to the performance in the 2-MWT, including aerobic fitness, functional and spatial-temporal gait parameters. Seventeen elderly adults performed a maximal incremental cycling test to determine maximum oxygen consumption (VO2max) and ventilatory thresholds (VT1 and VT2), two static standing balance tests with open and close eyes, a 5-times sit-to-stand test (5-TSTS), a handgrip test, and a 2-MWT on three different days over 2 weeks. No correlations were found between aerobic fitness indices and the distance covered in 2-MWT, but significant moderate correlations were found between the distance covered in 2-MWT and the time to perform the 5-TSTS (rho = -0.49) and with stride length (rho = 0.52) during the test. In conclusion, the 2-MWT does not seem a good test to assess aerobic capacity while it showed to be associated to the 5-TSTS performance of the elderly.
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Background: High-intensity intermittent training has emerged as an option for treating major depressive disorder (MDD). However, short sprint training (sSIT), an efficient HIIT modality, has not been tested yet for this purpose. The sSIT has been proven to induce the same metabolic adaptations, with the advantage of promoting lower muscle fatigue than other HIIT protocols. Methods: Seventeen adult women diagnosed with moderate/severe MDD were randomly allocated into a sSIT group (n=9) or a control condition (n=8). The sSIT group completed, over two weeks, six 6-10-min sessions which consisted of 3-12 "all out" sprints of 5 s interspersed with low-intensity recovery of 30-45 s. The week before and after the intervention, both groups were evaluated with the Hamilton Depression Rating Scale of 21-itens (HAM-D21), and for physical fitness and incidental physical activity. Results: The sSIT group exhibited significant improvements for HAM-D21 scores (24.6±8.2 vs. 16.8±10.1), maximum aerobic power (140±15 vs. 155±15 W), countermovement jump (13.0±3.4 vs. 14.9±3.1 cm), % of body fatness (32.4±4.4 vs. 29.3±3.8%), and 4-days number of steps (13,626±11,309 vs. 16,643±15,371) after the training period when compared to the control group. Conclusion: Less than 1 hour of a sSIT protocol over two weeks have demonstrated to reduce depressive symptoms, while improving aerobic fitness and body composition, and increasing incidental physical activity in a sample of women diagnosed with MDD.
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INTRODUCTION: In distance running, pacing is characterized by changes in speed, leading to runners dropping off the leader's pace until a few remain to contest victory with a final sprint. Pacing behavior has been well studied over the last 30 years, but much remains unknown. It might be related to finishing position, finishing time, and dependent on critical speed (CS), a surrogate of physiologic capacity. We hypothesized a relationship between CS and the distance at which runners "fell behind" and "let go" from the leader or were "outsprinted" as contributors to performance. METHODS: 100-m split times were obtained for athletes in the men's 10,000-m at the 2008 Olympics (N = 35). Split times were individually compared with the winner at the point of "falling behind" (successive split times progressively slower than the winner), "letting go" (large increase in time for distance compared with winner), or "outsprinted" (falling behind despite active acceleration) despite being with the leader with 400 m remaining. RESULTS: Race times ranged between 26:55 and 29:23 (world record = 26:17). There were 3 groups who fell behind at â¼1000 (n = 11), â¼6000 (n = 16), and â¼9000 m (n = 2); let go at â¼4000 (n = 10), â¼7000 (n = 14), and â¼9500 m (n = 5); or were outkicked (n = 6). There was a moderate correlation between CS and finishing position (r = .82), individual mean pace (r = .79), "fell behind" distance (r = .77), and "let go" distance (r = .79). D' balance was correlated with performance in the last 400 m (r = .87). CONCLUSIONS: Athletes displayed distinct patterns of falling behind and letting go. CS serves as a moderate predictor of performance and final placing. Final placing during the sprint is related to preservation of D' balance.
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Desempenho Atlético , Comportamento Competitivo , Corrida , Humanos , Masculino , Corrida/fisiologia , Desempenho Atlético/fisiologia , Comportamento Competitivo/fisiologia , Aceleração , Adulto , Fatores de TempoRESUMO
ABSTRACT There are a plethora of studies that have analyzed the effects of different resistance training methods on muscle hypertrophy. Recent studies have pointed out some potential advantage of training using cluster sets (CS) compared with traditional sets. It is still unclear whether CS are an effective method. The objective of this review was to investigate and discuss the current knowledge about the effect of CS on muscle hypertrophy. Four studies investigating the effect of CS on muscle hypertrophy were found. These studies demonstrated that CS induced similar or lower muscle hypertrophy than traditional sets. Thus, CS may lead to muscle hypertrophy, but did not provide a superior stimulus when compared to traditional sets of equated load.
RESUMO Um conjunto de estudos que tem analisado o efeito de diferentes métodos de treinamento resistido na hipertrofia muscular. Estudos têm pontuado várias potenciais vantagens do treinamento usando séries em conglomerados (SC) quando comparado com séries tradicionais. Ainda não está claro se as SC é um método efetivo. O objetivo desta revisão foi investigar e discutir o conhecimento recente sobre o efeito das SC na hipertrofia muscular. Quatro estudos investigando o efeito das SC na hipertrofia muscular foram encontrados. Esses estudos demonstraram que as SC induziram similar ou menor hipertrofia muscular do que séries tradicionais. Portanto, as SC podem induzir hipertrofia, porém não fornecem um estímulo superior quando comparado às séries tradicionais com carga equiparada.
RESUMEN Muchos estudios han analizado los efectos de diferentes métodos de entrenamiento de la fuerza en la hipertrofia muscular. Algunos estudios han resaltado las ventajas de introducir períodos de recuperación intra-serie (series cluster, SC) al compararlo con las series tradicionales. No está todavía claro si las SC son un método efectivo. El objetivo de esta revisión fue investigar y discutir el conocimiento actual sobre el efecto de las SC en la hipertrofia muscular. Se encontraron cuatro estudios investigando el efecto de las SC en la hipertrofia. Estos estudios demuestran que las SC inducen igual o menor hipertrofia que las series tradicionales. Así, las SC podrían inducir hipertrofia muscular pero no proporcionan un estímulo superior al compararlas con las series tradicionales.
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Este estudo analisou as respostas de pressão arterial sistólica (PAS) e pressão arterial diastólica (PAD) após duas sessões de exercício concorrente realizado em diferentes ordens [aeróbio-força (AF), e força-aeróbio (FA)]. Quinze indivíduos normotensos foram submetidos a duas sessões de exercício realizadas em dias distintos na seguinte sequência AF e FA. A PAS e PAD foram medidas antes e a cada 15 min durante 60 min de recuperação pós-exercício. Houve hipotensão pós-exercício (HPE) para PAS, aos 30 min (-7,4 mmHg), 45 min (-12,14 mmHg) e 60 min (-15,14 mmHg) de recuperação na sessão AF. Já na FA houve HPE apenas aos 60 min (-8,34 mmHg) de recuperação. A variação da PAS e PAD entre as sessões revelou HPE maior aos 15 min, 45 min e 60 min na PAS; e aos 45 min na PAD comparando-se AF a FA. A realização de exercício aeróbio antes do de força resultou em maior HPE para adultos jovens.
The study analyzed systolic (SBP) and diastolic (DBP) blood pressure after two exercise bouts of concurrent exercise performed in a different order [aerobic-strength (AS), and strength-aerobic (SA)]. Fifteen normotensive subjects were submitted to two exercise bouts performed in different days in the following order: AS and SA. SBP and DBP were measured before and at each 15 min over a 60 min period of post-exercise recovery. Post-exercise hypotension (PEH) was found in SBP at the 30th (-7.4 mmHg), 45th (-12.14 mmHg) and 60th min (-15.14 mmHg) after AS when compared to rest. On SA, PEH was only observed at the 60th min (-8.34 mmHg) of recovery. The analysis of differences for SBP and DBP between both sessions showed greater PEH at the 15th, 45th, and 60th min in SBP; and at the 45th min in DBP after AS when compared to SA. Thus, performing aerobic before strength exercise elicited a higher PEH in young adults.
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Humanos , Masculino , Feminino , Adulto , Pressão Arterial , Exercício Físico/fisiologia , HipotensãoRESUMO
We tested the hypothesis that: basal cardiac autonomic modulation (i.e. heart rate variability, HRV) was associated with the parasympathetic withdrawal during, and overall Yo-Yo IR1 performance; and therefore HRV could be used as a simple tool for specific fitness assessment. Nine sub-elite soccer referees performed the Yo-Yo IR1 until exhaustion with simultaneous recording of heart rate with the autonomic response during exercise calculated as the area under the curve (AUC). In the same week but with at least 48 hrs between days, a night-time HR recording was obtained from participants for determination of basal HRV. Correlation analysis demonstrated strong (r>0.83) relationships among Yo-Yo IR1 performance, AUC and basal HRV. These results indicate that both nocturnal HRV and AUC can be utilised as simple and valid monitoring tools of soccer referees' fitness.
Nós testamos a hipótese de: que a modulação autonômica cardíaca (i.e. variabilidade da freqüência cardíaca, VFC) está associada com a retirada parassimpática durante a execução do Yo-Yo IR1; e que, portanto, a VFC poderia ser usada como uma ferramenta simples para a avaliação do desempenho físico específico. Nove árbitros sub-elite de futebol executaram o Yo-Yo IR1 até a exaustão com registro simultâneo da freqüência cardíaca e VFC para o cálculo da área sob a curva (ASC). Na mesma semana, mas com mínimo de 48 horas entre dias, obteve-se um registro noturno da FC dos participantes para a determinação da VFC basal. As análises de correlação demonstraram elevados coeficientes (r>0,83) entre o desempenho no Yo-Yo IR1, a ASC e a VFC basal. Estes resultados indicam que a VFC noturna e a ASC podem ser empregadas como ferramentas simples e válidas para o monitoramento do desempenho de árbitros de futebol.
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Humanos , Masculino , Adulto , Desempenho Atlético/fisiologia , Futebol/fisiologiaRESUMO
FUNDAMENTO: O gene da enzima conversora de angiotensina (gene ECA) tem sido amplamente estudado em relação a fenótipos de aptidão cardiorrespiratória, contudo a associação do genótipo da ECA com corridas de meia-distância tem sido pouco investigada. OBJETIVO: O presente estudo investigou a possível influência da enzima conversora de angiotensina (ECA) (I/D) sobre a aptidão cardiovascular e o desempenho em corridas de meia-distância por parte de brasileiros jovens do sexo masculino. A validade da previsão de VO2max em relação ao genótipo da ECA também foi analisada. MÉTODOS: Um grupo homogêneo de homens jovens moderadamente ativos foi avaliado em um teste de corrida (V1600 m; m.min-1) e em um teste adicional em esteira ergométrica para a determinação de VO2max. Posteriormente, o [(0,177*V1600m) + 8.101] VO2max real e previsto foi comparado com os genótipos da ECA. RESULTADOS: O VO2max e V1600m registrados para os genótipos DD, ID e II foram 45,6 (1,8); 51,9 (0,8) e 54,4 (1,0) mL.kg-1.min-1 e 211,2 (8,3); 249,1 (4,3) e 258,6 (5,4 ) m.min-1, respectivamente e foram significativamente mais baixos para os genótipos DD (p < 0,05). O VO2max real e previsto não diferiram entre si, apesar do genótipo da ECA, mas o nível de concordância entre os métodos de VO2max real e estimado foi menor para o genótipo DD. CONCLUSÃO: Concluiu-se que existe uma possível associação entre o genótipo da ECA, a aptidão cardiovascular e o desempenho em corridas de média distância de jovens do sexo masculino moderadamente ativos e que a precisão da previsão do VO2max também pode ser dependente do genótipo da ECA dos participantes.
BACKGROUND: The angiotensin I-converting enzyme gene (ACE gene) has been broadly studied as for cardiorespiratory fitness phenotypes, but the association of the ACE genotype to middle-distance running has been poorly investigated. OBJECTIVE: This study investigated the possible influence of Angiotensin-Converting Enzyme (ACE) genotype (I/D) on cardiovascular fitness and middle-distance running performance of Brazilian young males. The validity of VO2max to predict the ACE genotype was also analyzed. METHODS: A homogeneous group of moderately active young males were evaluated in a 1,600 m running track test (V1600m; m.min-1) and in an incremental treadmill test for VO2max determination. Subsequently, the actual and the predicted [(0.177*V1600m) + 8.101] VO2max were compared to ACE genotypes. RESULTS: The VO2max and V1600m recorded for DD, ID and II genotypes were 45.6 (1.8); 51.9 (0.8) and 54.4 (1.0) mL.kg-1.min-1 and 211.2 (8.3); 249.1 (4.3) and 258.6 (5.4) m.min-1 respectively, and were significantly lower for DD carriers (p< 0.05). The actual and predicted VO2max did not differ from each other despite ACE genotype, but the agreement between actual and estimated VO2max methods was lower for the DD genotype. CONCLUSION: It was concluded that there is a possible association between ACE genotype, cardiovascular fitness and middle-distance running performance of moderately active young males and that the accuracy of VO2max prediction may also depend on the ACE genotype of the participants.