Production of Biosurfactants by Ascomycetes.

Surfactants are utilized to reduce surface tension in aqueous and nonaqueous systems. Currently, most synthetic surfactants are derived from petroleum. However, these surfactants are usually highly toxic and are poorly degraded by microorganisms. To overcome these problems associated with synthetic surfactants, the production of microbial surfactants (called biosurfactants) has been studied in recent years. Most studies investigating the production of biosurfactants have been associated mainly with bacteria and yeasts; however, there is emerging evidence that those derived from fungi are promising. The filamentous fungi ascomycetes have been studied for the production of biosurfactants from renewable substrates. However, the yield of biosurfactants by ascomycetes depends on several factors, such as the species, nutritional sources, and environmental conditions. In this review, we explored the production, chemical characterization, and application of biosurfactants by ascomycetes.

MLST reveals a clonal population structure for Cryptococcus neoformans molecular type VNI isolates from clinical sources in Amazonas, Northern-Brazil.

Cryptococcosis is considered endemic in Amazonas state, occurring more frequently in individuals with AIDS, who are predominantly infected by Cryptococcus neoformans molecular type VNI. Infections by Cryptococcus gattii VGII predominate in immunocompetent hosts from the American continent and are associated with outbreaks in North America, particularly the subtypes VGIIa and VGIIb, which are also present in the Brazilian Amazon region. Despite few environmental studies, several aspects of the molecular epidemiology of this disease in Amazonas remain unclear, including the limited use of multilocus sequence typing (MLST) to evaluate the genetic population structure of clinical isolates, mainly C. neoformans. Therefore, we used MLST to identify the sequence types of 38 clinical isolates of C. neoformans VNI and C. gattii VGII and used phylogenetic analysis to evaluate their genetic relationship to global isolates. Records of 30 patients were analyzed to describe the current scenario of cryptococcosis in the region and their associations with the different subtypes. Broth microdilution was also performed to determine the susceptibility profile to the antifungals amphotericin B, fluconazole and itraconazole. MLST identified that patients with HIV (n = 26) were exclusively affected by VNI strains with ST93, and among the VGII strains (n = 4), three STs (ST5, ST172 and the new ST445) were identified. An in-hospital lethality of 54% was observed in the HIV group, and there were no significant differences in the clinical aspects of the disease between the HIV and non-HIV groups of patients. In addition, all isolates were susceptible to the antifungals tested. Therefore, in Amazonas state, VNI isolates are a genetically monotypic group, with ST93 being highly important in HIV individuals.