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1.
FEMS Microbiol Lett ; 180(1): 7-14, 1999 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-10547438

RESUMO

Hemolysin II gene from Bacillus cereus VKM-B771 has been sequenced. The deduced primary translation product consists of 412 amino acid residues which corresponds to the protein with an M(r) of 45.6 kDa. The predicted mature Hly-II protein (residues 32 to 412) is of 42.3 kDa, which is in close agreement with the mini-cell electrophoresis analysis. Hly-II deletion variant lacking 96 C-terminal residues still has hemolytic activity. The protein primary structure analysis revealed no homology with any known Bacillus cytolysins. Significant general homology (31-28% identity) was found between the hemolysin II and Staphylococcus aureus alpha-toxin, gamma-hemolysin (HlgB), and leukocidins (LukF, LukF-R, LukF-PV). The data suggest that hemolysin II belongs to the group of beta-channel forming cytolysins.


Assuntos
Bacillus cereus/genética , Proteínas de Bactérias/genética , Genes Bacterianos , Sequência de Aminoácidos , Bacillus cereus/química , Sequência de Bases , Proteínas Hemolisinas , Leucocidinas/genética , Dados de Sequência Molecular , Alinhamento de Sequência , Staphylococcus aureus/química , Staphylococcus aureus/genética , Fosfolipases Tipo C/genética
2.
Arch Microbiol ; 161(3): 252-7, 1994.
Artigo em Inglês | MEDLINE | ID: mdl-8161285

RESUMO

To investigate the distribution of the hemolysin II determinant among strains of Bacillus cereus and Bacillus thuringiensis, thirteen strains of B. cereus and fourteen strains of B. thuringiensis strains were tested for hybridization of their chromosomal DNAs with a DNA probe containing the B. cereus hemolysin II gene. In addition, the production of hemolysin II, whose activity is not inhibited by cholesterol, was tested. The presence (absence) of the hydridization response in the microorganisms's genome correlated with the presence (absence) of cholesterol-unaffected hemolysin production. Only four out of thirteen B. cereus strains were found to give a positive response in hybridization experiments, whereas thirteen out of fourteen B. thuringiensis strains responded positively. DNAs from ten B. thuringiensis strains contained a 3.5 kb EcoRV fragment, which hybridized with the B. cereus hemolysin II gene probe. The 3.5 kb EcoRV DNA fragment from one of these strains (B. thuringiensis VKM-B1555) was cloned and expressed in Escherichia coli cells. The hemolysin encoded by the cloned DNA fragment was not inhibited by cholesterol and possessed all other properties of B. cereus hemolysin II. The obtained data clearly show limited distribution of hemolysin II among B. cereus strains and demonstrate that hemolysin II is more characteristic of B. thuringiensis than B. cereus.


Assuntos
Bacillus cereus/genética , Bacillus thuringiensis/genética , Proteínas Hemolisinas/genética , Bacillus cereus/química , Bacillus cereus/metabolismo , Bacillus thuringiensis/química , Bacillus thuringiensis/metabolismo , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/química , Colesterol , Clonagem Molecular , Sondas de DNA , DNA Bacteriano/genética , Genes Bacterianos , Proteínas Hemolisinas/biossíntese , Proteínas Hemolisinas/química , Peso Molecular , Hibridização de Ácido Nucleico , Especificidade da Espécie
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