Different litter compositions influence broiler chicken locomotion.

Background: Bedding material must absorb moisture, reduce impacts, and allow chicken to express their natural behavior reducing the occurrence of injuries in the footpad and joints, and improving carcass quality and performance. Aims: This study evaluated different bedding materials with different levels of inclusion of dried grass (Zoysia japonica) on the development of lesions in the locomotor system of broiler chickens. Methods: One thousand eight-day-old male chicks of the Cobb 500® were distributed in a completely randomized design with a 3 × 2 factorial scheme: three levels of grass inclusion and two bedding materials (100% wood shavings; 100% rice husks; 25% grass and 75% wood shavings; 25% grass and 75% rice husks; 50% grass and 50% wood shavings; 50% grass and 50% rice husks). The birds at 21, 28, 35, and 42 days of age were evaluated for pododermatitis. Ten birds from each repetition were identified and evaluated weekly from 21 to 42 days for Gait score, latency to lie and leg angle (valgus and varus). At 43 days of age, 60 birds of each treatment were slaughtered and evaluated for femoral degeneration and tibial dyschondroplasia. Results: Different litter compositions did not affect the incidence of lameness, tibial dyschondroplasia and spondylolisthesis. Pododermatitis scores increased after 28 days when including 50% of grass. Conclusion: Age contributes more to the development of locomotion injuries than does bedding material. It is recommended to use inclusions of dried grass only as bedding for young broilers.

Parâmetros zootécnicos e desenvolvimento da moela de frangos de corte em dietas de sorgo grão e a inclusão de carotenoides / Zootechnical parameters and gizzard development of broilers fed with sorghum grain and carotenoid supplementation

Objetivou-se avaliar a substituição do milho pelo sorgo grão, moído ou inteiro, e a inclusão de carotenoides sintéticos em rações de frangos de corte, sobre parâmetros zootécnicos e desenvolvimento da moela. Utilizaram-se 960 frangos, machos e fêmeas, da linhagem Cobb 500, que foram submetidos a quatro dietas: à base de milho (M), à base de sorgo moído (SM) ou ainda acrescido de carotenoides sintéticos (SMC) e à base de sorgo inteiro (SI). Foram avaliados peso vivo, consumo de ração, conversão alimentar, rendimento de carcaça e cortes, peso de moela. A ração de SI proporcionou maior desenvolvimento de moela, embora, aos sete dias, não tenha havido diferenças no peso vivo e na conversão alimentar entre as dietas. SM e SMC proporcionaram maiores valores de peso vivo aos 28 dias do que SI. As conversões alimentares real e tradicional aos 40 dias foram semelhantes para as rações de sorgo, com um melhor rendimento de carcaça em SM e SMC. O sorgo moído pode substituir integralmente o milho nas rações para frangos de corte, sem comprometimento de desempenho e rendimento de carcaça, e, na forma grão inteiro, é zootecnicamente viável.(AU)

This study aimed to evaluate the replacement of corn with ground or whole grain sorghum and the inclusion of carotenoids in broiler feed on zootechnical parameters and gizzard development. A total of 960 Cobb 500 broilers, half of each sex, were submitted to four feeds: ground corn (M), ground sorghum (SM) ground sorghum with carotenoid supplementation (SMC) and whole grain sorghum (SI). Body weight, feed intake and feed conversion, carcass and cuts yield, gizzard weight were evaluated. SI feed provided a greater development of gizzard, however at 7 days there were no differences in body weight and feed conversions between diets. SM and SMC provided higher values of body weight at 28 days than SI feed. Feed conversion at 40 days were the same for the sorghum feeds with a better carcass yield in SM and SMC. Ground sorghum can completely replace corn in broiler diets without compromising performance and carcass yield, and whole grain sorghum is zootecnaly viable.(AU)

Antibody response to the 43 kDa glycoprotein of Paracoccidioides brasiliensis as a marker for the evaluation of patients under treatment.

Sera of patients with paracoccidioidomycosis contained IgG-, IgA-, and IgM-specific antibodies to a 43 kDa antigen contained in the filtrate of a culture of Paracoccidioides brasiliensis. IgG- and IgA-specific antibodies were present in all observed patients. The IgM response was more frequent in acute cases, and the mean titers of IgG- and IgM-specific antibodies were higher in the acute forms. By the fourth month of chemotherapy, there was a decay of IgG, IgA, and IgM antibody titers to this antigen in acute cases, correlating with clinical improvement. The detection of IgG and IgA antibodies and the sequential determination of antibodies to the 43 kDa glycoprotein may be useful tools for serodiagnosis and evaluation of therapeutic efficacy.

Efeito de diferentes tempos de exposição ao calor e de linhagens sobre o rendimento de carcaça e a composição química de peito de frangos de corte / Effect of different timing of exposure to heat and strains on the carcass yield and breast chemical composition of broiler

Investigaram-se os efeitos de diferentes tempos de exposição ao calor sobre o rendimento de carcaça, cortes e vísceras comestíveis e a composição química da carne de peito de frangos de corte de duas linhagens. Foram alojados 560 pintos de corte da linhagem Cobb 500 Slow® e 560 da Hubbard Flex® em 32 boxes. A partir do 14º dia, eles foram divididos nos ambientes térmicos: controle, uma hora, duas horas ou três horas diárias de estresse por calor. O rendimento de carcaça, peito sem osso, coxa e sobrecoxa com osso, asas, pés, fígado, moela e coração foi avaliado em seis aves de cada linhagem em cada ambiente térmico. Amostras foram obtidas para analisar matéria seca, extrato etéreo, proteína bruta e matéria mineral. O estresse cíclico por calor por até três horas diárias não influenciou o rendimento de carcaça, de peito sem osso, de coxa e sobrecoxa, de asa, de coração, de moela, de fígado e a composição química do peito. A linhagem Cobb 500 Slow® apresentou maior rendimento de peito. A Hubbard Flex® apresentou peito com maior percentual de matéria mineral e proteína. A linhagem Cobb é mais indicada à produção de peito, e a linhagem Hubbard à produção de frango inteiro.(AU)

The effects of different exposure periods under heat on the carcass yield, cuts and edible organs and breast chemical composition of broilers from two strains were investigated. Five hundred and sixty Cobb 500 Slow® and 560 Hubbard Flex® broiler chicks were housed in 32 pens and from the 14th day on, they were divided in thermal environments: control, 1 hour, 2 hours or 3 hours a day under heat stress. Carcass yield, boneless breast, thigh and drumstick with bone, wings, feet, liver, gizzard and heart were evaluated in six birds of each strain in each thermal environment. Samples were obtained to assess dry matter, ether extract, crude protein and mineral matter. The cyclic heat stress for up to three hours a day did not influence 33 the carcass yield, boneless breast, thigh and drumstick, wing, heart, gizzard, liver, and the breast chemical composition. Cobb 500 Slow® strain had higher breast yield. Hubbard Flex® had breast with higher percentage of mineral matter and protein. Cobb strain is best designed to breast production, and Hubbard is best for whole chicken production.(AU)

Detection of the 43,000-molecular-weight glycoprotein in sera of patients with paracoccidioidomycosis.

The 43,000-molecular-weight (43K) soluble glycoprotein was detected in sera of patients with paracoccidioidomycosis by the immunoblot technique by using as the probe rabbit monospecific antisera to this fraction. The 43K antigen was present before treatment in sera of patients with the acute (juvenile) form; it started to disappear from circulation after 10 months of chemotherapy, and it was undetectable after 2 years of treatment. In the chronic cases, the 43K antigen was detected in patients without treatment, and it was absent in the healed cases. The detection of the 43K protein specific to Paracoccidioides brasiliensis may be important for its diagnostic value as well as for modulation of the host immune response.

Monocyte adherence to Paracoccidioides brasiliensis, zymosan-C3b and erythrocyte-hemolysin in patients with paracoccidioidomycosis.

Independent and dependent (C3b/Fc receptors) opsonic adherence ability of monocytes from thirty-three patients with acute or chronic paracoccidioidomycosis and from 13 healthy individuals were studied in the presence of Paracoccidioides brasiliensis (Pb), Paracoccidioides brasiliensis opsonized by patient's serum (PbPS) or normal serum (PbNS), zymosan opsonized by fresh sera from healthy donors (ZyNS) and erythrocytes opsonized by hemolysin (EA). Statistically significant differences concerning the percentage of adhered monocytes to PbPS (number of adhered monocytes/total number of monocytes) were detected between control and chronic (active and inactive) groups. Significant differences in relationship to the mean number of PbPS (number of fungi in monocytes/total number of monocytes) were also observed between control and chronic active mycosis. Present data suggest that patients with chronic disease have more ability in the first step of phagocytic activity, considered as the main effector mechanism to control the dissemination and severity of paracoccidiodomycosis.

IgG, IgM and IgA antibody response for the diagnosis and follow-up of paracoccidioidomycosis: comparison of counterimmunoelectrophoresis and complement fixation.

IgG, IgM and IgA antibodies to GP43 (glycoprotein fraction of Paracoccidioides brasiliensis) were measured by ELISA in 63 samples from 23 patients with paracoccidioidomycosis before and twice after chemotherapy was started. Antibodies against P. brasiliensis were detected by indirect immunofluorescence (IF) (IgG, IgM and IgA isotypes), counterimmunoelectrophoresis (CIE) and complement fixation. Two control groups composed of 19 healthy individuals and 12 patients with other diseases (six with histoplasmosis, three with tuberculosis and three with other mycoses). The highest efficiency percentages were found with IgG and IgA-ELISA (100%), IgG-IF (96.2%), CIE (94.4%) and the lowest with CF (75.9%). Highest positive and negative predictive values (100%) were observed for IgG and IgA ELISA. IgG and IgM-ELISA antibodies are more often found in patients with acute than chronic disease (P = 0.01). Four to six months after treatment follow-up showed decreased levels of IgG and IgM-ELISA for acute cases and decreased titres of CIE for chronic cases in relation to pretreatment levels. This study suggests that IgG-ELISA anti-GP43 represents a good marker to monitor clinical response to therapy.