RESUMO
The small RNA (sRNA) RydC strongly activates cfa, which encodes the cyclopropane fatty acid synthase. Previous work demonstrated that RydC activation of cfa increases the conversion of unsaturated fatty acids to cyclopropanated fatty acids in membrane lipids and changes the biophysical properties of membranes, making cells more resistant to acid stress. The regulators that control RydC synthesis had not previously been identified. In this study, we identify a GntR-family transcription factor, YieP, that represses rydC transcription. YieP positively autoregulates its own transcription and indirectly regulates cfa through RydC. We further identify additional sRNA regulatory inputs that contribute to the control of RydC and cfa. The translation of yieP is repressed by the Fnr-dependent sRNA, FnrS, making FnrS an indirect activator of rydC and cfa. Conversely, RydC activity on cfa is antagonized by the OmpR-dependent sRNA OmrB. Altogether, this work illuminates a complex regulatory network involving transcriptional and post-transcriptional inputs that link the control of membrane biophysical properties to multiple environmental signals. IMPORTANCE: Bacteria experience many environmental stresses that challenge their membrane integrity. To withstand these challenges, bacteria sense what stress is occurring and mount a response that protects membranes. Previous work documented the important roles of small RNA (sRNA) regulators in membrane stress responses. One sRNA, RydC, helps cells cope with membrane-disrupting stresses by promoting changes in the types of lipids incorporated into membranes. In this study, we identified a regulator, YieP, that controls when RydC is produced and additional sRNA regulators that modulate YieP levels and RydC activity. These findings illuminate a complex regulatory network that helps bacteria sense and respond to membrane stress.
Assuntos
Proteínas de Escherichia coli , Escherichia coli , Regulação Bacteriana da Expressão Gênica , RNA Bacteriano , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas de Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , RNA Bacteriano/metabolismo , RNA Bacteriano/genética , Transcrição Gênica , Pequeno RNA não Traduzido/genética , Pequeno RNA não Traduzido/metabolismo , Ácido Graxo Sintases/metabolismo , Ácido Graxo Sintases/genética , Fatores de Transcrição/metabolismo , Fatores de Transcrição/genética , Ciclopropanos , Ácidos Graxos , MetiltransferasesRESUMO
Maize starch is an important carbohydrate source in human diet, and its digestion contributes to the postprandial blood glucose level. This article describes in vitro starch digestibility and its relation to endosperm hardness and composition in cooked maize flours. Starch digestion and estimated glycemic index (GI) were significantly (p < 0.05) lower in hard endosperm genotypes (65.1 and 77.3, respectively) than in soft ones (70.7 and 80.7, respectively), and they were negatively correlated (p < 0.05) with specific zein concentrations (total zeins, Z1, Z2, and C1, E, and F zeins). Cooking with sodium sulfite significantly (p < 0.001) increased starch hydrolysis in all genotypes (â¼13%), evidencing the impact of disulfide bonds on this attribute. Explored amylose:starch ratios did not impact starch digestibility. Regardless of hardness, fine grinding significantly (p < 0.001) increased total starch digestibility in >30%. Our results focus on specific kernel physicochemical traits for developing maize food products with lower starch digestibility and GI.