New evidence on variations of human body burden of methylmercury from fish consumption.

Epidemiologic studies commonly use mercury (Hg) level in hair as a valid proxy to estimate human exposure to methylmercury (MeHg) through fish consumption. This study presents the results yielded by a complete data set on fish consumption habits, Hg levels in edible fish resources, and corresponding Hg accumulation in hair, gathered in three distinct communities of eastern Canada. For one of these communities, the average hair Hg concentration was 14 times less than the expected value based on calculated daily oral exposure and current knowledge of MeHg metabolism. This finding could be explained by differences in specific genetic characteristics and/or interactive effects of other dietary components.

Evaluation of two current approaches for the measurement of carbon dioxide diffusive fluxes from lentic ecosystems.

The dry ice sowing experiment (DISE) consisted in adding dry ice to a lake and monitoring the subsequent evasion of carbon dioxide (CO2). DISE allowed us to evaluate two approaches commonly used for measuring aquatic CO2 diffusive fluxes: the boundary layer equation (BLE) from Cole and Caraco (1998) and a particular model of static chamber (SC). CO2 evasion measurements with both approaches were compared to CO2 mass budgets as a relative reference to define their recovery coefficients (p). p for the BLE and the SC over the whole measurement period were 101 +/- 14% and 115 +/- 56%, respectively. Results from discrete sampling intervals revealed that the BLE generally provided estimations in good agreement (80-130%) with the mass budgets during both daytime and nighttime. Variations in p for the BLE were related to wind speed and, consequently, piston velocity (k600). The SC overestimated CO2 evasion during daytime (149 +/- 39%), and underestimated it during nighttime (57 +/- 18%). Variations in p for the SC were related to k600, stemming mainly from the alteration of the air/ water temperature gradient.

Toxicological effects of methylmercury on walleye (Sander vitreus) and perch (Perca flavescens) from lakes of the boreal forest.

Biochemical and physiological responses of walleye (Sander vitreus) and perch (Perca flavescens) were studied in four Canadian boreal forest lakes representing a mercury (Hg) exposure gradient. The aim of this study was to assess the effects of Hg and methylmercury (MeHg) on the general physiological condition of fish as well as to gauge the relationship between MeHg and the glutathione (GSH) system in metal-contaminated and reference sites using a series of biomarkers. Walleye from Lake Malartic had the highest liver MeHg concentrations, exhibited lower hepatosomatic indices (HSI) and lower glutathione S-transferase (GST) activity. HSI was negatively related to liver total Hg concentrations in walleye (R2=0.33, n=108, P<0.0001). Glutathione reductase (GR) and GST activity for walleye from Lake Malartic were related to HSI (R2=0.38, n=25, P=0.0010; R2=0.46, n=27, P<0.0001, respectively). In Lake Desjardins-East, where perch had the highest liver MeHg concentrations, glutathione peroxidase selenium dependent activity (GSH-Px SD) and GST activity were negatively related to liver MeHg concentrations (R2=0.39, n=21, P=0.0026; R2=0.22, n=21, P=0.0298, respectively). This study suggests that Hg may induce adverse effects on the physiology and cellular metabolism of walleye and perch at environmentally relevant concentrations.

New evidence on the effects of tea on mercury metabolism in humans.

The authors present the results of an experiment in which they explored the role of tea in human metabolic processing of methylmercury (MeHg) from fish consumption. The experiment involved 50 scientists from the Collaborative Mercury Research Network (COMERN) who agreed to eat fish for 2 daily meals for 3 consecutive days. Half of the participants also drank 6 cups of tea daily, starting a week before and continuing through the experiment. The authors calculated the total amount of MeHg that each participant ingested from (1) the measured mercury (Hg) level in fish and (2) the quantity of fish eaten, and compared it with the total increases of Hg and MeHg levels in participants' blood. Results indicated that the control group metabolized roughly 100% of the available fish MeHg, whereas the tea-exposed group showed blood levels of MeHg at more than 40% than that available in the fish provided, suggesting that an external MeHg pool supplied part of the measured blood MeHg increase. The authors conclude that tea may accelerate the enterohepatic MeHg cycle and contribute to a temporary bioamplification of MeHg in the bloodstream.