RESUMO
Relatively little is known about the genes and brain structures that enable virgin female Drosophila to make the decision to mate or not. Classical genetic approaches have identified several mutant females that have a reluctance-to-mate phenotype, but most of these have additional behavioral defects. However, the icebox (ibx) mutation was previously reported to lower the sexual receptivity of females, without apparently affecting any other aspect of female behavior. We have shown that the ibx mutation maps to the 7F region of the Drosophila X chromosome to form a complex complementation group with both lethal and viable alleles of neuroglian (nrg). The L1-type cell adhesion molecule encoded by nrg consists of six immunoglobulin-like domains, five fibronectin-like domains, one transmembrane domain and one alternatively spliced intracellular domain. The ibx strain has a missense mutation causing a glycine-to-arginine change at amino acid 92 in the first immunoglobulin domain of nrg. Defects in the central brain of ibx mutants are similar to those observed in another nrg mutant, central brain deranged(1) (ceb(1)). However, both ceb(1) homozygous and ceb(1)/ibx heterozygous females are receptive. The expression of a transgene containing the non-neural isoform of nrg rescues both the receptivity and the brain structure phenotypes of ibx females.
Assuntos
Moléculas de Adesão Celular Neuronais/genética , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Mutação , Comportamento Sexual Animal/fisiologia , Cromossomo X , Animais , Encéfalo/metabolismo , Encéfalo/patologia , Moléculas de Adesão Celular Neuronais/fisiologia , Mapeamento Cromossômico , Proteínas de Drosophila/fisiologia , Drosophila melanogaster/fisiologia , Feminino , Teste de Complementação Genética , Masculino , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Fenótipo , Isoformas de Proteínas , Fatores SexuaisAssuntos
Febre Hemorrágica da Crimeia/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Vírus da Febre Hemorrágica da Crimeia-Congo , Febre Hemorrágica da Crimeia/etiologia , Febre Hemorrágica da Crimeia/fisiopatologia , Febre Hemorrágica da Crimeia/transmissão , Humanos , Controle de Infecções/métodos , Masculino , Pessoa de Meia-Idade , Turquia/epidemiologiaRESUMO
The purpose of this study was to determine Bartonella henselae prevalance in cats in Ankara. Whole bloods and sera collected from 256 cats were investigated for the presence feline Bartonella species by culture and sera were tested for the presence of antibodies against B. henselae IgG using immunofluorescence assay. Bartonella species were isolated by blood culture from 24 (9.4%) cats. Bartonella isolates were subjected to restriction fragment length polymorphism (RFLP) by using TaqI and HhaI endonucleases to identify species. Twenty-one isolates were determined as B. henselae and three of 24 isolates were determined as Bartonella clarridgeiae with RFLP. The bacteraemia prevalence and seroprevalence of B. henselae IgG antibodies in cats was detected as 8.2% and 18.6% respectively. This is the first report on B. henselea and B. clarridgeiae in cats in Turkey.