Pseudophoenix ekmanii (Arecaceae) seeds at suboptimal temperature show reduced imbibition rates and enhanced expression of genes related to germination inhibition.

Pseudophoenix ekmanii is a critically endangered palm species that can be found in the southeast of the Dominican Republic. The temperatures to which P. ekmanii seeds are exposed upon dispersal range from 32 to 23 °C (max and min) and can reach a low of approximately 20 °C in January. Our aim was to analyse the effect of suboptimal (20 °C) and optimal (30 °C) germination temperature on seed imbibition, moisture content, embryo growth and gene expression patterns in this tropical palm species. Seed imbibition was tracked using whole seeds, while moisture content was assessed for individual seed sections. Embryo and whole seed size were measured before and after full imbibition. For transcriptome sequencing, mRNA was extracted from embryo tissues only and the resulting reads were mapped against the Elaeis guineensis reference genome. Differentially expressed genes were identified after statistical analysis and subsequently probed for enrichment of Gene Ontology categories 'Biological process' and 'Cellular component'. Our results show that prolonged exposure to 20 °C slows whole seed and embryo imbibition and causes germination to be both delayed and inhibited. Embryonic transcriptome patterns associated with the negative regulation of germination by suboptimal temperature include up-regulation of ABA biosynthesis genes, ABA-responsive genes, as well as other genes previously related to physiological dormancy and inhibition of germination. Thus, our manuscript provides the first insights into the gene expression patterns involved in the response to suboptimal temperature during seed imbibition in a tropical palm species.

Comparison of seed and seedling functional traits in native Helianthus species and the crop H. annuus (sunflower).

Seed functional traits of native Helianthus species contribute towards ecosystem services but limitations to their use in managed programmes exist. Many perennial Helianthus possess seed dormancy. The ability for germination to occur under different temperature and drought conditions, as well as the capacity of germinated seeds to convert into normal seedlings is rarely considered. Our aim was to identify and quantify these constraints through functional trait analyses. In five seed lots of native Helianthus (four perennial and one annual) and five genotypes of sunflower (H. annuus) for comparison, dormancy, thermal and hydro thresholds and times, morphology, mass, oil content and conversion into normal seedlings were quantified. The influence of the seed collection site environment on these traits was also explored. Seed dormancy of the perennial species was overcome by scarification followed by germination in 5 mm GA3 . Thermal and hydro-time analyses revealed slower germination for the native seed lots (>1350 °Ch) in comparison to the sunflower genotypes (<829.9 °Ch). However, native seed lots had a higher capacity to convert into normal seedlings at high temperatures and low water potentials than sunflower genotypes. For the native seed lots, the average monthly temperature of the collection site was negatively correlated with thermal time. Variability in seed functional traits of native Helianthus and greater capacity for germinated seeds to convert into normal seedlings suggests they are better equipped to cope with high temperature and drought scenarios than sunflower. Effective dormancy alleviation is required to facilitate the use of native Helianthus species.