RESUMO
During 2012, a novel pandemic GII.4 norovirus variant, Sydney 2012, emerged worldwide. A signature of the variant was a GII.Pe ORF1, in association with GII.4 Apeldoorn 2008-like ORF2-ORF3 genes. We report the detection of recombinant GII.4 Sydney 2012 strains, possessing the ORF1 gene of the former pandemic variant New Orleans 2009.
Assuntos
Infecções por Caliciviridae/virologia , Norovirus/classificação , Norovirus/genética , Recombinação Genética , Infecções por Caliciviridae/epidemiologia , Criança , Pré-Escolar , Humanos , Dados de Sequência Molecular , Norovirus/isolamento & purificação , Fases de Leitura Aberta , Pandemias , RNA Viral/genética , Análise de Sequência de DNARESUMO
Infection by a novel canine astrovirus was associated with gastroenteritis in two dogs. The virus displayed 70.3 to 73.9% amino acid identity to other canine astroviruses in the full-length capsid. Specific antibodies were detected in the convalescent-phase sera of the dogs, indicating seroconversion. Also, the virus appeared weakly related antigenically to the prototype canine astrovirus isolate ITA/2008/Bari.
Assuntos
Infecções por Astroviridae/veterinária , Doenças do Cão/diagnóstico , Doenças do Cão/virologia , Gastroenterite/veterinária , Mamastrovirus/classificação , Mamastrovirus/isolamento & purificação , Animais , Anticorpos Antivirais/sangue , Infecções por Astroviridae/diagnóstico , Infecções por Astroviridae/patologia , Infecções por Astroviridae/virologia , Proteínas do Capsídeo/genética , Análise por Conglomerados , Doenças do Cão/patologia , Cães , Gastroenterite/diagnóstico , Gastroenterite/patologia , Gastroenterite/virologia , Dados de Sequência Molecular , Filogenia , RNA Viral/genética , Análise de Sequência de DNARESUMO
Different strategies have been proposed to overcome maternally derived antibody (MDA) interference with canine parvovirus type 2 (CPV-2) immunisation, including intranasal vaccination, which presents some practical limitations. In the present study, the results of the oral administration of a commercial CPV-2b modified live virus (MLV) vaccine in pups with MDA are reported. The CPV-2b vaccine was orally administered to 14 6-week-old pups with a bait. Blood samples and rectal swabs were collected at different days post-vaccination (dpv) to determine CPV-2 antibody titres and DNA loads. Thirteen pups were positive to serological and virological tests after the first vaccination and one pup became positive after the second vaccine administration. The findings of this study suggest that systemic immunity against CPV-2 may be achieved by the use of an MLV CPV-2b vaccine administered orally even in the presence of MDA titres that usually interfere with vaccination.
Assuntos
Doenças do Cão/prevenção & controle , Infecções por Parvoviridae/prevenção & controle , Parvovirus Canino/imunologia , Vacinas Virais/administração & dosagem , Administração Oral , Animais , Doenças do Cão/imunologia , Cães , Feminino , Masculino , Infecções por Parvoviridae/imunologia , Infecções por Parvoviridae/veterinária , Vacinação/veterinária , Vacinas Virais/imunologiaRESUMO
Hepatitis A virus (HAV) was detected in a batch of imported non-packaged frozen redcurrants purchased in a Bari grocery. Sequence and phylogenetic analysis showed the HAV strain clustered tightly with the HAV strain from the 2013 Italian epidemic, providing additional evidence that frozen redcurrants were the main vehicle of the HAV outbreak.
Assuntos
Frutas/virologia , Vírus da Hepatite A/isolamento & purificação , Hepatite A/virologia , Ribes/virologia , Surtos de Doenças , Frutas/economia , Hepatite A/epidemiologia , Vírus da Hepatite A/classificação , Vírus da Hepatite A/genética , Humanos , Itália/epidemiologia , Dados de Sequência Molecular , Filogenia , Polônia , RNA Viral/genéticaRESUMO
Global surveillance for norovirus identified in 2012 the emergence of a novel pandemic GII.4 variant, termed Sydney 2012. In Italy, the novel pandemic variant was identified as early as November 2011 but became predominant only in the winter season 2012-2013. Upon sequencing and comparison with strains of global origin, the early Sydney 2012 strains were found to differ from those spreading in 2012-2013 in the capsid (ORF2) putative epitopes B, C and D, segregating into a distinct phylogenetic clade. At least three residues (333, 340 and 393, in epitopes B, C and D, respectively) of the VP1 varied among Sydney 2012 strains of different clades. These findings suggest that the spread of the pandemic variant in Italy during the winter season 2012-2013 was due to the introduction of strains distinct from those circulating at low frequency in the former winter season and that similar strains were also circulating elsewhere worldwide.