Antibacterial efficacy and mechanisms of action of low power atmospheric pressure cold plasma: membrane permeability, biofilm penetration and antimicrobial sensitization.

AIM: The objective of this study was to determine the efficacy and mechanisms of inactivation of two clinically relevant ESKAPE bacteria namely Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus by atmospheric pressure cold plasma. METHODS AND RESULTS: Plasma was generated between two brass grids by applying a radiofrequency electric field to a flow of helium. Intracellular generation of reactive species, alterations in cell membrane, and inactivation of bacteria in planktonic or biofilm growth were studied. Results were compared with commonly used antimicrobial drugs. Plasma exposure generated reactive oxygen and nitrogen species in bacteria, disrupted membrane integrity and reduced bacterial load. The efficacy in bacterial inactivation was comparable to antibiotics but exhibited a quicker killing rate. The antibacterial effect of plasma synergistically increased in association with antibiotics and did not diminish over repeated exposures, suggesting no development in bacterial resistance. CONCLUSIONS: Through generation of reactive species, cold plasma altered cell membrane and effectively inactivated clinically important bacteria, both in suspension and in biofilms. SIGNIFICANCE AND IMPACT OF THE STUDY: As cold plasma damages different targets in bacterial cells, it emerges as an effective strategy used alone or in combination with antimicrobial drugs to control microbial infections and prevent the selection of resistant bacterial strains.

Experimental observation of microtearing modes in a toroidal fusion plasma.

Experimental evidences of short wavelength electromagnetic modes are found in the reversed-field-pinch configuration device RFX-mod by means of in-vessel magnetic probes. The modes are revealed during the helical states of the plasma. Their amplitude is well correlated to the electron temperature gradient strength in the core. On the basis of linear gyrokinetic calculations we interpret these instabilities as microtearing modes.

Direct two-dimensional measurements of the field-aligned current associated with plasma blobs.

In simple magnetized toroidal plasmas, field-aligned blobs originate from ideal interchange waves and propagate radially outward under the effect of ∇B and curvature induced E×B drifts. We report on the first experimental two-dimensional measurements of the field-aligned current associated with blobs, whose ends terminate on a conducting limiter. A dipolar structure of the current density is measured, which originates from ∇B and curvature induced polarization of the blob and is consistent with sheath boundary conditions. The dipole is strongly asymmetric due to the nonlinear dependence of the current density at the sheath edge upon the floating potential. Furthermore, we directly demonstrate the existence of two regimes, in which parallel currents to the sheath do or do not significantly damp charge separation and thus blob radial velocity.

Direct observation of current in type-I edge-localized-mode filaments on the ASDEX Upgrade tokamak.

Magnetically confined plasmas in the high confinement regime are regularly subjected to relaxation oscillations, termed edge localized modes (ELMs), leading to large transport events. Present ELM theories rely on a combined effect of edge current and the edge pressure gradients which result in intermediate mode number (n≅10-15) structures (filaments) localized in the perpendicular plane and extended along the field lines. By detailed localized measurements of the magnetic field perturbation associated to type-I ELM filaments, it is shown that these filaments carry a substantial current.

First operation in SPIDER and the path to complete MITICA.

The requirements of ITER neutral beam injectors (1 MeV, 40 A negative deuterium ion current for 1 h) have never been simultaneously attained; therefore, a dedicated Neutral Beam Test Facility (NBTF) was set up at Consorzio RFX (Padova, Italy). The NBTF includes two experiments: SPIDER (Source for the Production of Ions of Deuterium Extracted from Rf plasma), the full-scale prototype of the source of ITER injectors, with a 100 keV accelerator, to investigate and optimize the properties of the ion source; and MITICA, the full-scale prototype of the entire injector, devoted to the issues related to the accelerator, including voltage holding at low gas pressure. The present paper gives an account of the status of the procurements, of the timeline, and of the voltage holding tests and experiments for MITICA. As for SPIDER, the first year of operation is described, regarding the solution of some issues connected with the radiofrequency power, the source operation, and the characterization of the first negative ion beam.

Implementation of the new multichannel X-mode edge density profile reflectometer for the ICRF antenna on ASDEX Upgrade.

A new multichannel frequency modulated continuous-wave reflectometry diagnostic has been successfully installed and commissioned on ASDEX Upgrade to measure the plasma edge electron density profile evolution in front of the Ion Cyclotron Range of Frequencies (ICRF) antenna. The design of the new three-strap ICRF antenna integrates ten pairs (sending and receiving) of microwave reflectometry antennas. The multichannel reflectometer can use three of these to measure the edge electron density profiles up to 2 × 1019 m-3, at different poloidal locations, allowing the direct study of the local plasma layers in front of the ICRF antenna. ICRF power coupling, operational effects, and poloidal variations of the plasma density profile can be consistently studied for the first time. In this work the diagnostic hardware architecture is described and the obtained density profile measurements were used to track outer radial plasma position and plasma shape.

Fast Thermal Helium Beam diagnostic for measurements of edge electron profiles and fluctuations.

The edge of fusion experiments is a region where strong gradients develop, together with the presence of strong fluctuations due to turbulence. The thermal helium beam diagnostic developed for the RFX-mod experiment allows the measurements with a single diagnostic of both low frequency time evolution of the edge radial profiles of electron density and temperature (tens of hertz), and the high frequency fluctuations (hundreds of kHz). To maximize the collected light, the three HeI lines necessary to be measured for the evaluation of n(e) and T(e) are separated with a spectrograph, and multianode photomultipliers are used as light detectors. The paper describes the diagnostic setup, with the interface hardware with the machine and the optical layout, and the characterization of its performances.

Optical measurements for turbulence characterization in RFX-mod edge.

The edge of the reversed field pinch experiment RFX-mod (R=2 m, a=0.46 m) is characterized by a complex magnetic topology and strong pressure gradients. A set of fast spectroscopic diagnostics has been designed to characterize both main plasma edge parameters and turbulence, including coherent structures (blobs) and radial profiles of the thermodynamic variables. Measurements of the edge radial profiles of electron density (n(e)), temperature (T(e)), and pressure (P(e)) are obtained by a thermal helium beam diagnostic, which locally measures the ratios of the spectral lines emitted by a neutral He cloud puffed into hydrogen plasma discharges. A gas puff imaging system allows the high frequency (1 MHz bandwidth) measurement of the fluctuations of He I emissivity in the same positions. The system measures both the intermittent behavior of the edge turbulence and n(e), T(e), and P(e) profiles of the corresponding coherent structures, with a resolution down to 6 µs. The optical diagnostics are complemented by a set of triaxial magnetic coils to simultaneously characterize the edge magnetic field.

Direct measurement of current filament structures in a magnetic-confinement fusion device.

Turbulent structures detected in the edge plasma of fusion devices, often described as blobs, are generally believed to be responsible for confinement degradation. Recent experimental evidence and theories have suggested their filamentary electromagnetic nature. In this Letter the first direct experimental measurements of the parallel current density associated with turbulent structures in a fusion experiment are reported. The electromagnetic nature of structures is clearly shown by identifying the current filaments with a vortexlike velocity pattern and the associated pressure perturbation.

Active-feedback control of the magnetic boundary for magnetohydrodynamic stabilization of a fusion plasma.

Stable operation with control on magnetohydrodynamic modes has been obtained in the modified reversed field experiment employing a set of 192 feedback controlled saddle coils. Improvements of plasma temperature, confinement (twofold), and pulse length (threefold) and, as a consequence of the magnetic fluctuation reduction, strong mitigation of plasma-wall interaction and mode locking are reported.

Self-regulation of E x B flow shear via plasma turbulence.

The momentum balance has been applied to the ExB flow in the edge region of a reversed field pinch (RFP) configuration. All terms, including those involving fluctuations, have been measured in stationary condition in the edge region of the Extrap-T2R RFP experiment. It is found that the component of the Reynolds stress driven by electrostatic fluctuations is the term playing the major role in driving the shear of the ExB flow to a value marginal for turbulent suppression, so that the results are in favor of a turbulence self-regulating mechanism underlying the momentum balance at the edge. Balancing the sheared flow driving and damping terms, the plasma viscosity is found anomalous and consistent with the diffusivity due to electrostatic turbulence.

Kink instability in applied-field magneto-plasma-dynamic thrusters.

Measurements of magnetic and electrostatic fluctuations in an applied field magneto-plasma-dynamic thruster have shown that a m/n=1/1 kink mode becomes unstable whenever the Kruskal-Shafranov limit is violated. A positive correlation is established between the kink and performance degradation at high current, which has until now prevented the use of this kind of thruster in space missions.

Vortex-induced diffusivity in reversed field pinch plasmas.

Coherent structures identified in two reversed field pinch experiments are interpreted as a dynamic balance of dipolar and monopolar vortices growing and evolving under the effect of the ExB flow shear. For the first time their contribution to the anomalous transport has been estimated in fusion related plasmas, showing that they can account for up to 50% of the total plasma diffusivity. The experimental findings indicate that the diffusion coefficient associated with the coherent structures depends on the relative population of the two types of vortices and is minimum when the two populations are equal. An interpretative model is proposed to explain this feature.

Search of self-organized criticality processes in magnetically confined plasmas: hints from the reversed field pinch configuration.

In order to test the self-organized criticality (SOC) paradigm in transport processes, a novel technique has been applied for the first time to plasmas confined in reversed field pinch configuration. This technique consists of an analysis of the probability distribution function of the times between bursts in density fluctuations measured by microwave reflectometry and electrostatic probes. The same analysis has also been applied to intermittent events sorted out from the Gaussian background. In both cases, the experimental results disagree with the predictions for a SOC system.

Transport processes in reversed-field-pinch plasmas: inconsistency with the self-organized-criticality paradigm.

A statistical analysis of the anomalous particle flux in the edge region of the RFX experiment has revealed that laminar times between bursts, which account for more than 50% of the losses, have a power law distribution and that flux fluctuations are not self-similar. These properties are found in contrast with a wide class of self-organized-criticality models so that it is concluded that there is no experimental evidence of avalanchelike process occurrence in the plasma of RFX.