Functional activities of the upper extremity of young adults with cerebral palsy: a limiting factor for participation?

PURPOSE: To assess functional activities of the upper extremity of young adults with cerebral palsy (CP) and to determine their relations with participant characteristics and participation. METHOD: Assessment of functional activities of the upper extremity was performed on 103 participants (aged 16-20 years) with the Melbourne assessment and the Abilhand Questionnaire. Participation was measured with the Life Habits Questionnaire. Participant characteristics included age, gender, limb distribution of the spastic paresis, educational level and gross and fine motor function. Relations among these variables were studied by means of correlation coefficients and linear regression analysis. RESULTS: Limitations in functional activities of the upper extremity were related to the limb distribution of the spastic paresis and were especially present in quadriplegic participants. Significant correlations between participant characteristics and measures of functional activities were present. Limitations in functional activities of the upper extremity, measured with the Abilhand Questionnaire, were an important determinant for participation, in addition to the gross motor function and educational level. CONCLUSION: Limitations in functional activities of the upper extremity are an important determinant for restrictions in participation in young adults with CP. It is recommended to include assessment of functional activities of the upper extremity in patients with CP.

Expression Profile of MicroRNA Biogenesis Components in Renal Transplant Patients.

BACKGROUND: MicroRNAs (miRNAs) and the miRNA biogenesis components are potential biomarkers of some prevalent diseases, such as cancer and diabetes. In light of this information, we aimed to investigate the expression profiles of miRNA biogenesis components in renal transplant patients before and after transplantation and how these profiles are related to immunosuppressive treatment and clinical outcomes of these patients. METHODS: In this study, gene and protein expression profiles of Dicer, Drosha, Pasha (DGCR8), Exportin5 (XPO5), and Argonaute2 (AGO2) in peripheral blood mononuclear cells (PBMCs) of renal transplant patients were evaluated by means of real-time quantitative polymerase chain reaction and Western blot methods before and 3 months after transplantation. Patients who had transplant procedures for the first time were included in the study. RESULTS: Gene expressions were significantly reduced after transplantation. The reduction rate of expressions in 1 patient undergoing chronic rejection was higher. In addition, in patients under everolimus treatment, gene expression of Dicer did not change and gene expression of AGO2 increased. Dicer, Drosha, DGCR8, and AGO2 protein expressions were reduced in all patients, but no change was observed in XPO5 protein expression in nonrejecting patients. Interestingly, in the patient undergoing chronic rejection, protein expression profiles other than Dicer were distinctive from nonrejecting patients. However, XPO5 protein expression was higher in that patient. CONCLUSIONS: Our study shows the importance of the global effect of immunosuppressive treatment on the miRNA biogenesis pathway. miRNA biogenesis components are potential biomarkers indicative of graft outcome and pharmacologic target molecules.