RESUMO
UNLABELLED: In this work we describe the case report of a woman affected by cancer of the sigmoid colon and with a positive direct antiglobulin test (DAT) and indirect antiglobulin test (IAT). Case report with results: A meticulous medical history showed that the woman had been suffering from recurrent fetal loss. Then she had cardiac and coagulative problems. These data suggested a phospholipid syndrome. CONCLUSION: The patient had a medical history positive for a phospholipid syndrome and we think that this disease could explain the onset of the autoantibody.
Assuntos
Anticorpos/sangue , Colo Sigmoide/imunologia , Colo Sigmoide/patologia , Neoplasias do Colo Sigmoide/diagnóstico , Neoplasias do Colo Sigmoide/imunologia , Aborto Habitual , Idoso de 80 Anos ou mais , Anemia Hemolítica Autoimune/complicações , Síndrome Antifosfolipídica/complicações , Autoanticorpos/sangue , Transtornos da Coagulação Sanguínea/complicações , Doenças Cardiovasculares/complicações , Colonoscopia , Teste de Coombs , Feminino , HumanosRESUMO
INTRODUCTION: Nowadays transfusion safety is still put at risk by contamination of pathogens. The Mirasol PRT System blocks the replication of pathogens and white blood cells. Our goal was to quantify the activation of platelets after treatment with the Mirasol device. MATERIALS AND METHODS: From September to December 2013, 131 platelet collections were studied using a simple flow cytometric strategy. RESULTS: There was a significant correlation between the percentage of platelet activated before and after the treatment. CONCLUSION: Our results induced us to think that the activation of platelets after treatment was acceptable.
Assuntos
Plaquetas/metabolismo , Fármacos Fotossensibilizantes/farmacologia , Ativação Plaquetária , Riboflavina/farmacologia , Raios Ultravioleta , Adulto , Plaquetas/citologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ativação Plaquetária/efeitos dos fármacos , Ativação Plaquetária/efeitos da radiaçãoRESUMO
The aim of our study is to assess the mortality of leukocytes during extracorporeal photopheresis. Sixty-three photopheresis performed on 13 patients affected by chronic GvHD were evaluated. Samples were analyzed using a FACSCalibur flow cytometer. Apoptosis and necrosis of limphomononuclear cells dramatically increased after the apheretic procedure. We found a further increase of apoptotic and necrotic limphomononuclear cells after treatment with 8-MOP and UVA (p≤0.05). Our data suggested that the immunomodulatory effects of extracorporeal photopheresis, triggered by circulating apoptotic or necrotic cells, could play an important role in the treatment of GvHD with this procedure.