RESUMO
A bacterial strain, JH03(T), was isolated from gravel adjacent to Geommeolle beach on Udo Island, South Korea. The cells were Gram-stain-negative, aerobic, non-motile and rod shaped. The ranges of temperature, pH and NaCl concentration for growth of the bacterium were 10-45 °C, pH 6.0-9.5 and 0.5-5.0 % (w/v), respectively. The major fatty acids of the bacterium were iso-C(15:0) (15.4 %), iso-C(15:1) G (14.1 %), iso-C(16:0) 3-OH (14.1 %), iso-C(17:0) 3-OH (11.5 %) and anteiso-C(15:0) (11.3 %). The major isoprenoid quinone was MK-6. The polar lipids included phosphatidylethanolamine, two unidentified amino lipids and three unidentified lipids. The DNA G+C content was 34.2 mol%. The phylogenetic analysis of the 16S rRNA gene sequences showed that strain JH03(T) was most closely related to Jejuia pallidilutea EM39(T) (96.5 % sequence similarity). Based on the polyphasic analysis, strain JH03(T) is a novel species of the genus Jejuia, for which the name Jejuia marina sp. nov. is proposed. The type strain is JH03(T) (= KCTC 42342(T) = JCM 30601(T)).
Assuntos
Flavobacteriaceae/classificação , Sedimentos Geológicos/microbiologia , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , Ácidos Graxos/análise , Flavobacteriaceae/química , Flavobacteriaceae/genética , Flavobacteriaceae/crescimento & desenvolvimento , Concentração de Íons de Hidrogênio , RNA Ribossômico 16S/genética , República da Coreia , Salinidade , TemperaturaRESUMO
A Gram-stain negative, non-motile, rod-shaped and aerobic bacterial strain, designated JG48(T), was isolated from a gravel sample taken from a beach adjacent to Udo island, South Korea. Strain JG48(T) was found to grow optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. The 16S rRNA gene sequence of strain JG48(T) exhibited sequence similarities of 96.67 % to Hyunsoonleella jejuensis CNU004(T). The major fatty acids present in the strain JG48(T) were identified as iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH and iso-C15:0 3-OH. The predominant isoprenoid quinone was identified as MK-6. The polar lipids profile of strain JG48(T) was found to consist of phosphatidylethanolamine, three unidentified amino lipids and four unidentified lipids. The DNA G+C content of strain JG48(T) was determined to be 34 mol%. Based on the morphological and physiological properties, and the results of phylogenetic analyses, the strain is considered to represent a novel species of the genus Hyunsoonleella, for which the name Hyunsoonleella udoensis sp. nov. is proposed. The type strain is JG48(T) (=KCTC 42341(T)=JCM 30600(T)).