1.
Org Lett
; 8(7): 1319-22, 2006 Mar 30.
Artigo
em Inglês
| MEDLINE
| ID: mdl-16562881
RESUMO
[reaction: see text] Oxidosqualene-lanosterol cyclases convert oxidosqualene to lanosterol in yeast and mammals. Site-saturated mutants' construction of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase, at Trp232 exchanges against proteinogenic amino acids, and product profiles are shown. All mutants, except Lys and Arg, produced protosta-12,24-dien-3beta-ol, lanosterol, and parkeol. Overall, Trp232 plays a catalytic role in the influence of rearrangement process and determination of deprotonation position but does not involve intervention in the cyclization steps.
Assuntos
Transferases Intramoleculares/metabolismo , Saccharomyces cerevisiae/enzimologia , Saccharomyces cerevisiae/genética , Triptofano/química , Arginina/química , Arginina/genética , Catálise , Ciclização , Transferases Intramoleculares/química , Transferases Intramoleculares/genética , Lisina/química , Lisina/genética , Oxirredução
2.
Chembiochem
; 6(7): 1177-81, 2005 Jul.
Artigo
em Inglês
| MEDLINE
| ID: mdl-15915534