Smoking cessation in Taiwanese male smokers with coronary artery disease: influencing factors and policy implications.

AIM: To investigate factors affecting smoking cessation in male smokers with coronary artery disease. DESIGN: A descriptive, correlation, cross-sectional study was conducted. METHODS: Data were collected using a questionnaire constructed by a literature review of research on smoking cessation in male smokers including demographics and smoking background, as well as psychological, interpersonal and environmental factors. A total of 130 male patients with coronary artery disease were recruited from the cardiac clinic at a regional hospital in Taiwan from August to December 2008. The response rate was 93% (n = 121). Descriptive statistics, chi-square, t-tests and logistic regression analysis were conducted. RESULTS: During the survey, 64.5% of the respondents reported that they had stopped smoking after a coronary event. Five factors were significantly associated with smoking cessation after diagnosis of coronary artery disease: age, the severity of heart diagnoses, antismoking norms (perceived that smoking was against the social norms), nicotine dependence level, and contrary views of smoking (perceived negative expectancy of smoking). Multivariate analysis revealed antismoking norms to be the most important predictor (AOR = 4.27; P < .05) after adjusting age. CONCLUSION: The study highlights the need to develop smoking cessation interventions that specifically counsel patients with coronary artery disease about these information, such as the disease risk and development, health consequences of smoking, and dependence therapy. Additionally, government support for tobacco control programmes in hospitals and elsewhere is designed to have a beneficial effect on patients' smoking behaviours primarily by increasing antismoking norms.

Rapid detection of equine infectious anaemia virus nucleic acid by insulated isothermal RT-PCR assay to aid diagnosis under field conditions.

BACKGROUND: Control of equine infectious anaemia (EIA) currently depends on serological diagnosis of infected equids. However, recently infected equids may not produce detectable anti-EIAV antibodies up to 157 days post infection and so present a high transmission risk. Therefore, direct nucleic acid detection methods are urgently needed to improve EIAV surveillance and management programs in counties where the disease is endemic. OBJECTIVES: To evaluate a field-deployable, reverse transcription-insulated isothermal PCR (RT-iiPCR) assay targeting the conserved 5' untranslated region (5' UTR)/exon 1 of the tat gene of EIAV. STUDY DESIGN: The analytical and clinical performance of the newly developed EIAV RT-iiPCR was evaluated by comparison with a EIAV real-time RT-PCR (RT-qPCR) along with the AGID test. METHODS: Analytical sensitivity was determined using in vitro transcribed RNA containing the target area of the 5' UTR/tat gene and samples from two EIAV-positive horses. Specificity was verified using nine common equine viruses. Clinical performance was evaluated by comparison with EIAV RT-qPCR and AGID using samples derived from 196 inapparent EIAV carrier horses. RESULTS: EIAV RT-iiPCR did not react with other commonly encountered equine viruses and had equivalent sensitivity (95% detection limit of eight genome equivalents), with a concordance of 95.41% to conventional EIAV RT-qPCR. However, the RT-qPCR and RT-iiPCR had sensitivities of 43.75 and 50.00%, respectively, when compared to the AGID test. MAIN LIMITATIONS: Low viral loads commonly encountered in inapparent EIAV carriers may limit the diagnostic sensitivity of RT-PCR-based tests. CONCLUSIONS: Although EIAV RT-iiPCR is not sufficiently sensitive to replace the current AGID test, it can augment control efforts by identifying recently exposed or "serologically silent" equids, particularly as the latter often represent a significant transmission risk because of high viral loads. Furthermore, the relatively low cost and field-deployable design enable utilisation of EIAV RT-iiPCR even in remote regions.

Acute and chronic effects of haloperidol on plasma and brain homovanillic acid in the rat.

The effects of acute and chronic administration of haloperidol on homovanillic acid (HVA) in plasma and the brain were examined in the rat. Acute haloperidol treatment (1 mg/kg) resulted in highly significant elevations in HVA within 30 min and produced a maximal increase of HVA in 3-6 hr in both plasma and the whole brain. The response of brain HVA to increasing doses (0.05-30 mg/kg) of haloperidol showed an inverted U pattern. Plasma HVA showed a very flat response to lower doses (less than or equal to 5 mg/kg) of haloperidol and a dramatically elevated one to higher doses (greater than or equal to 10 mg/kg). Haloperidol produced a parallel increase in plasma and brain HVA at lower doses (less than or equal to 2 mg/kg) only. After chronic administration of haloperidol for up to 28 days, the response of HVA in plasma correlated mainly with, but tolerated later than, those in the whole brain and the olfactory tubercle.

Product ion studies of diastereomeric benzo[ghi]fluoranthene tetraols by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and post-source decay.

The product ion formation characteristics of the four diastereomeric tetrahydroxy benzo[ghi]fluoranthene compounds formed by hydrolysis of the syn and anti diastereomers of trans-3,4-dihydroxy-5,5a-epoxy-3,4,5,5a-tetrahydrobenzo[ghi]fluoranthene are studied using matrix-assisted laser desorption/ionization and post-source decay (PSD) to determine a correlation between the fragmentation characteristics of these tetraols and the structures of the diol-epoxide diastereomers from which they are hydrolyzed. The tetraols formed by the trans ring opening of the diol epoxides during hydrolysis yield product ion spectra specific for the syn and anti configurations of their precursor diol epoxides. All four diastereomeric tetraols form product ions by the losses of one and/or two water molecules in varying proportions when lithium-cationized molecule ions (m/z 301) are selected for PSD product ion analysis. The differences in the PSD spectra of these four Li+-cationized molecules are rationalized in terms of a water loss mechanism that involves the 1,2 elimination of a hydrogen atom and hydroxyl group that are cis with respect to each other on adjacent carbons.

[Dento-facial structural characteristics in Angle Class II malocclusion associated with abnormal facial divergency].

Lateral cephalometric radiographs of 60 adult patients with Angle class 11 malocclusion associated with abnormal facial divergency were collected from the Orthodontic Department of the National Taiwan University Hospital. They were divided into a hyperdivergent group (35 cases) and a hypodivergent group (25 case), according to mandibular plane angle (SN-MP). The 19 landmarks on each cephalometric tracing were digitized into a computer, then computer-aided cephalometric analysis was performed to calculate the 17 skeletal measurements and 13 dentoalveolar measurements. The dento-facial structural characteristics of the hyperdivergent and hypodivergent groups were compared. It was found that the subjects of the hyperdivergent group revealed a greater tendency of divergency in the anterior cranial base plane, Frank-fort horizontal plane, palatal plane, occlusal plane, and mandibular plane. Hyperdivergent facial type, supposedly indicating an open bite or a tendency toward an open bite, has a longer lower anterior facial height, shorter posterior facial height, longer upper anterior and posterior dental height. While, the majority of dentofacial characteristics of the hypodivergent facial type observed in is study were directly opposite to those of the hyperdivergent facial type. The relationships of incisor overbite depth and other skeletal and dentoalveolar parameters were illustrated by Pearson's correlation coefficient and stepwise multiple regression analysis by means of the SPSS/PC statistic program. With the incisor overbite depth as the dependent variable, the independent variables included on the regression analysis were the 10 items of skeletal and dentoalveolar parameters. The compared parameters showed a statistically significant correlation with the incisor overbite depth (P < 0.001). By the stepwise method, the variables included on the regression equation were (1) N-Go-Gn, (2) A-Gn-Ar, (3) N-Ans/ans-Me, and (4) U1L1. The value of R square (R2) in the regression analysis was 0.543. It demonstrated that only a 54.3% variation in incisor overbite depth can be explained by variations in those skeletal and dentoalveolar variables.

Comparison of landmark identification in traditional versus computer-aided digital cephalometry.

The aim of this study was to assess landmark identification on digital images in comparison with those obtained from original radiographs. Ten cephalometric radiographs were selected randomly. Seven orthodontic residents identified 19 cephalometric landmarks on both the original radiographs and the digital images. To assess the concordance between landmarks identified on the original radiographs and on their digital counterparts, the x, y coordinates for each landmark in the 2 modalities were transformed with the identical references. The placement differences for 19 landmarks between 2 methods were calculated and their components in horizontal and vertical directions were analyzed respectively. Multivariate analysis of variance showed that the "cephalometric radiograph" and "landmark" variation had greater influence than that from "method" (landmark identification on digital / original radiograph). It was also noted that the differences of landmark identification between original radiographs and their digital counterparts were statistically significant. The landmarks with significant differences of horizontal component on the x-axis were Me, Gn, ANS, PNS, LIA. The differences were generally under 1 mm with the exception of Or, Me, PNS, LIA. The landmarks with significant differences of vertical component on the y-axis were Po, Or, Gn. The inter-observer error for each landmark in digital images was generally larger than that in the original radiographs. However, statistically significant differences of inter-observer errors between 2 modalities were only found for 4 of the 19 landmarks. These 4 landmarks, Po, Ar, ANS, and UM, should be scrutinized more carefully during potential applications of digital cephalometry.

Correlation of cervical vertebra maturation with hand-wrist maturation in children.

The purpose of this study was to evaluate the reliability of cervical vertebra maturation as an indicator of skeletal age during the circumpubertal period. This was determined by correlating cervical vertebra maturation to hand-wrist maturation. The vertebral skeletal age was assessed using lateral cephalometric radiographs according to maturity indicators modified from Lamparski. The hand-wrist skeletal age was evaluated in radiographs with the system developed by Fishman. The sample consisted of 503 subjects (244 boys and 259 girls), aged 8 through 18 years. The Spearman rank correlation coefficients and Wilcoxon sign rank test showed that a statistically significant relationship existed between the two assessments. Both the intra- and inter-judge tests of reliability displayed no significant differences. The results of this study indicate that skeletal age assessment made from the maturational changes of cervical vertebrae were reliable, reproducible and valid.

Angular distribution of polarized photon-pairs in a scattering medium with a Zeeman laser scanning confocal microscope.

A novel confocal microscope designed for use with turbid media is proposed. We use a Zeeman laser as the light source. Based on the properties of two-frequency polarized photon-pairs and the common-path feature of polarized photon-pairs with heterodyne detection employed in the proposed confocal microscope, three gatings (spatial filtering gating, polarization gating and spatial coherence gating) are thus simultaneously incorporated in the microscope. Experimental results for the angular distribution of polarized photon-pairs in a scattering medium indicate that polarization gating and spatial coherence gating preclude the detection of multiply scattered photons, whereas the pinhole selects the least scattered photon-pairs. Thus, better performance for axial resolution than can be obtained with a conventional confocal microscope is demonstrated experimentally. In addition, the proposed microscope is able to either look deeper into a turbid medium or work with a denser medium; furthermore, the axial resolution is improved.

Effects of instantaneous prestretching on force degradation characteristics of orthodontic plastic modules.

The object of this study was to investigate the effect of instantaneous prestretching on the force degradation behavior of three kinds of four-link plastic modules. A simple sliding apparatus was used to prestretch instantaneously the plastic modules to 100, 200 and 300% of their original length. After prestretching, specimens were tested at 20, 25 and 30 mm stretch distances. Force measurements were performed at the following occasions: before prestretching; at the end of prestretching; after 1, 4 and 24 hours; and after 1, 2 and 3 weeks. The following conclusions can be drawn from this investigation: The control specimens which had not been instantaneously prestretched exhibited considerable force loss over the three-week test period. Most of the force loss occurred rapidly during the first few hours, with a much lower subsequent force degradation rate. Instantaneous prestretching to a greater length resulted in a lower force value remaining at the end of prestretching. Instantaneous prestretching of the plastic modules is an efficient method to dissipate the high initial force level which is clinically undesirable. Prestretching of the modules around or slightly longer than the stretch distance could reduce the high initial force level and still maintain the same force level as the control groups. However, if the length of instantaneous prestretching of the four-link plastic module was much longer than the testing stretch distance which corresponds to the average distance from the first molar to the canine in the same quadrant, the prestretching would reduce both the high initial force value and the force value during the three-week test period, as compared with the control group.

The effects of lead ion on immune function of rabbit alveolar macrophages: quantitation of immune phagocytosis and rosette formation by 51Cr in vitro.

Experiments by a 51Cr-labeling technique were performed to investigate the effects of lead (Pb2+) on immune phagocytosis and Fc-rosette formation of rabbit pulmonary alveolar macrophages (PAMs). Evidence is presented that Pb2+ at concentrations of 10(-3) and 10(-4)M could inhibit these functions of PAMs. The degree of inhibition corresponded to the concentration of this heavy metal ion in vitro.

The relationship of dental crowding to tooth size, dental arch width, and arch depth.

This study was undertaken to answer the question, "Are there any differences in the mesiodistal tooth diameters, dental arch width and dental arch depth between the crowded and noncrowded dentitions?" The materials submitted for study consisted of the Orthoscan intraoral occlusograms of 89 subjects with well-aligned dentition and those of 74 subjects with gross dental crowding. The tooth size and arch dimensions were measured from these intraoral occlusograms. The data were subjected to statistical treatment for comparison of noncrowded and crowded groups. The findings in this study lead to the following conclusions: The mesiodistal tooth diameters of the crowded group were significantly larger than those of the noncrowded group. The dental arch widths of the crowded group were significantly smaller than those of the noncrowded group. No substantial or significant difference in dental arch depth between the crowded and noncrowded subjects could be noted. From these findings it seems that tooth size and dental arch width are determining factors in the formation of dental crowding. Clinically, these results suggest that in the treatment of dental crowding, extraction of teeth and/or expansion of dental arches should be considered depending on what is the main causative factor.

Group learning factors in a problem-based course in oral radiology.

OBJECTIVES: To assess the development of group dynamics in students who participated in a problem-based learning (PBL) course in oral radiology and to evaluate the influence of the facilitators' experience. METHODS: At the beginning and end of the course, students in three consecutive years (1997, 1998 and 1999) responded to a group learning factor questionnaire designed to measure group dynamics (engagement, interpersonal relationships, lack of commitment and disruptive behaviour, interpersonal learning and self-presentation). The answers were analysed with paired t-test and the effects of the facilitator's seniority, expertise in oral radiology, experience of PBL and single or rotating facilitators assessed. RESULTS: Most groups in the three years reported above average group dynamic scores. There were significant changes in group dynamics. In the 1997 class, interpersonal relationships improved (P=0.014). In the 1998 class, interpersonal learning (P=0.021) and engagement (P=0.009) increased significantly, while disruptive behaviour decreased (P=0.031). In the 1999 class, lack of commitment and disruptive behaviour decreased (P=0.014). Groups led by a junior faculty member with no previous experience of PBL show less commitment and more disruptive behaviour. CONCLUSIONS: More commitment and less disruptive behaviour and improved engagement, interpersonal relationships and self-presentation were found in a PBL course in oral radiology. Facilitators should have prior exposure to PBL to avoid disruptive behaviour among the students.

A cross-sectional study on the skeletal development of the hand and wrist from preadolescence to early adulthood among Chinese in Taiwan.

In order to study the skeletal development of the hand-wrist among Chinese in Taiwan, a total of 1,679 hand-wrist radiographs were collected from the files in the Division of Orthodontics, School of Dentistry, National Taiwan University and the Orthodontic Clinic of the National Taiwan University Hospital. The ages of the subjects ranged from 6.3 years to 22.7 years. The nine commonly used ossification events, PP2 = ,MP3 = ,S, MP3cap, DP3u, MP3u, Riu, Rau and Rcu, were used in this study. It was found that the sequence of the nine skeletal maturation stages did not differ between males and females and was generally in agreement with similar studies among other ethnic groups. A statistically significant difference did exist between the males and females in the mean ages of onset of the skeletal maturation stages. All of the skeletal stages were more advanced in the females than in the males. The mean ages of the nine skeletal maturation stages obtained in this study could be used as a reference for daily pedodontic and orthodontic practice as well as for related studies. Although there was no intention of making a statistical comparison of the data for the Chinese with those of other ethnic groups, some diagrammatic illustrations for comparison were made.

The influence of dental occlusion on electromyographic silent periods.

Electromyographic silent periods (EMG SP) and occlusal contact intensity were studied in 31 young patients (9-18 years of age) having different types of malocclusion. EMG SP was induced from bilateral temporalis and masseter muscles by chin tapping during isometric contraction. The simple silent period (SSP), depressed activity (DP) and double silent period (2SP or 3SP) were classified according to the character of silence. The occlusal contact intensity was obtained by using a polymer wafer for clenching and was analysed with a photocclusion analyser. It was found that the average amplitude of temporalis and masseter muscles during clenching was about 400 microV. The mean SSP was around 34 msec. The total SP was around 40 msec in temporalis and 44 msec in masseter. All of the subjects demonstrated one or more DP in ten taps while 18 of them had 2SP or 3SP. The difference in contact intensity between right and left had a strong correlation with the EMG SSP+DP, SSP+2SP3SP and total SP. Anterior-posterior difference was not as strongly related to SP parameters. Less significant correlation was found between SSP and occlusal intensity. It is concluded that the influence of occlusion on EMG SP is basically related to the uneven occlusal contact between the two sides rather than the total occlusal contact intensity or the difference in intensity anterior-posteriorly.

Thin-plate spline (TPS) graphical analysis of the mandible on cephalometric radiographs.

We describe two cases of Class III malocclusion with and without orthodontic treatment. A thin-plate spline (TPS) analysis of lateral cephalometric radiographs was used to visualize transformations of the mandible. The actual sites of mandibular skeletal change are not detectable with conventional cephalometric analysis. These case analyses indicate that specific patterns of mandibular transformation are associated with Class III malocclusion with or without orthopaedic therapy, and visualization of these deformations is feasible using TPS graphical analysis.

Beyond synonymy: exploiting the UMLS semantics in mapping vocabularies.

The Unified Medical Language System (UMLS) contains semantic information about terms from various sources, each concept can be understood and located by its relationships to other concepts: this is a result of the organizing principle of semantic locality. We describe a method in which the semantic relationships between concepts are used to map concepts from different vocabularies in the UMLS. Applied to mapping concepts to MeSH, this method is able to map 50 to 65% of the non-MeSH concepts to MeSH. A manual review of the mapping shows a relevance rate of 61%. Causes of failure include a lack of consistently represented relationships in the UMLS, and some inconsistencies in the categorization of the concepts. The limits of this method are discussed, as well as possible adaptations for other uses.

Observation of bolus position with standardized test foods and fluoroscopic technique.

Sugar and agar balls containing barium were made for the fluoroscopic observation of the bolus position during habitual side chewing in 13 adult males (aged from 20 to 28 years) who had a complete dentition and healthy masticatory system. The test foods had constant size, shape, weight and known hardness, i.e. type 1, 20 kg; type 2, 40 kg; type 3, 1.0 kg and type 4, 0.5 kg. These four types of test foods were chewed randomly in a fluoroscopic room. The jaw separation distance and the bolus position during first bite and crushing bite were observed. The influence of the chewing order of four types of test foods on the bolus position was also recorded. It was found that both hard and soft test foods were most often crushed at the first molar area, and the separation distance during the crushing bite was only slightly wider than the diameter of the food. The first bite jaw separation distance for hard food chewing was significantly wider than soft food chewing and the later in the chewing order the wider the separation distance. The effect of chewing order was not significant on the position and jaw separation distance of the crushing bite.