RESUMO
We have cloned and sequenced the p53-encoding cDNA of rainbow trout (Salmo gairdneri). The encoded product contains the characteristics found in all p53 proteins: (i) the five highly conserved domains, (ii) an acidic N terminus, (iii) a hydrophilic C terminus, and (iv) a penultimate serine residue. Furthermore, we demonstrate that the rainbow trout p53 is able to specifically interact with the SV40 large T antigen.
Assuntos
Genes p53/genética , Truta/genética , Proteína Supressora de Tumor p53/metabolismo , Sequência de Aminoácidos , Animais , Antígenos Transformantes de Poliomavirus/metabolismo , Sequência de Bases , Evolução Biológica , Clonagem Molecular , Sondas de DNA/genética , Humanos , Dados de Sequência Molecular , Proteína Supressora de Tumor p53/química , Proteína Supressora de Tumor p53/genéticaRESUMO
Two cases of temporary loss of reflex erectile activity after transurethral bladder-neck and external sphincter resection are reported in two tetraplegics. Only recently such occurrences have been reported in the literature associated with external sphincterotomy. The reasons are discussed and most likely direct damage to erectile tissues is the cause of such a complication.
Assuntos
Disfunção Erétil/etiologia , Complicações Pós-Operatórias , Uretra/cirurgia , Bexiga Urinaria Neurogênica/cirurgia , Adulto , Humanos , Masculino , Traumatismos da Medula Espinal/complicações , Bexiga Urinaria Neurogênica/etiologiaRESUMO
A new non-isotopic sandwich hybridization assay was developed to detect human immunodeficiency virus type 1 (HIV1) provirus amplified by the polymerase chain reaction. The sensitivity and specificity of this new technique using 96-well microplates as the support for the sandwich hybridization procedure and stable enzyme-linked oligomer as the detection probe were compared with those of Southern hybridization using a 32P-labelled oligomer probe. Three laboratories studied 437 peripheral blood mononuclear cell samples from 294 different subjects including both HIV1-seropositive and -seronegative individuals. The non-isotopic assay exhibited a sensitivity of 99.5% and a specificity of 99.1% when compared with the Southern procedure. In addition, the non-isotopic assay gives clear numeric data, is safe when handling, and is especially adapted to large-scale analyses.