RESUMO
BACKGROUND: Melatonin has been shown with anticancer property and therapeutic potential for tumors. However, there lacks a systematic study on the molecular pathways of melatonin and its antitumor effects in gastrointestinal carcinomas. METHODS: Using the gene expression profiles of four cancer cell lines from three types of gastrointestinal carcinomas before and after melatonin treatment, including gastric carcinoma (GC), colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC), differentially expressed genes (DEGs) and biological pathways influenced by melatonin were identified. The qRT-PCR analyses were performed to validate the effects of melatonin on 5-FU resistance-related genes in CRC. RESULTS: There were 17 pathways commonly altered by melatonin in the three cancer types, including FoxO signaling pathways enriched by the upregulated DEGs and cell cycle signaling pathways enriched by the downregulated DEGs, confirmed the dual role of melatonin to tumor growth, pro-apoptosis and anti-proliferation. DEGs upregulated in the three types of cancer tissues but reversely downregulated by melatonin were commonly enriched in RNA transport, spliceosome and cell cycle signaling pathways, which indicate that melatonin might exert antitumor effects through these pathways. Our results further showed that melatonin can downregulate the expression levels of 5-FU resistance-related genes, such as thymidylate synthase in GC and ATR, CHEK1, BAX and MYC in CRC. The qRT-PCR results demonstrated that melatonin enhanced the sensitivity of CRC 5-FU resistant cells by decreasing the expression of ATR. CONCLUSIONS: Melatonin exerts the effects of pro-apoptosis and anti-proliferation on gastrointestinal carcinomas, and might increase the sensitivity of 5-FU in GC and CRC patients.
Assuntos
Carcinoma Hepatocelular , Neoplasias Hepáticas , Melatonina , Linhagem Celular Tumoral , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Melatonina/farmacologia , TranscriptomaRESUMO
A new calcium-modified and starch-stabilized ferromanganese binary oxide (Ca-SFMBO) sorbent was fabricated with different Ca concentrations for the adsorption of arsenic (As) and cadmium (Cd) in water. The maximum As(III) and Cd(II) adsorption capacities of 1% Ca-SFMBO were 156.25 mg/g and 107.53 mg/g respectively in single-adsorption systems. The adsorption of As and Cd by the Ca-SFMBO sorbent was pH-dependent at values from 1 to 7, with an optimal adsorption pH of 6. In the dual-adsorbate system, the presence of Cd(II) at low concentrations enhanced As(III) adsorption by 33.3%, while the adsorption of As(III) was inhibited with the increase of Cd(II) concentration. Moreover, the addition of As(III) increased the adsorption capacity for Cd(II) up to two-fold. Through analysis by X-ray photoelectron spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FTIR), it was inferred that the mechanism for the co-adsorption of Cd(II) and As(III) included both competitive and synergistic effects, which resulted from the formation of ternary complexes. The results indicate that the Ca-SFMBO material developed here could be used for the simultaneous removal of As(III) and Cd(II) from contaminated water.
Assuntos
Arsênio , Poluentes Químicos da Água , Purificação da Água , Adsorção , Cádmio , Cálcio , Concentração de Íons de Hidrogênio , Ferro , Cinética , Manganês , Óxidos , Amido , ÁguaRESUMO
BACKGROUND: Currently, pathological examination of gastroscopy biopsy specimens is the gold standard for gastric cancer (GC) diagnosis. However, it has a false-negative rate of 10-20% due to inaccurate sampling locations and/or insufficient sampling amount. A signature should be developed to aid the early diagnosis of GC using biopsy specimens even when they are sampled from inaccurate locations. METHODS: We extracted a robust qualitative transcriptional signature, based on the within-sample relative expression orderings (REOs) of gene pairs, to discriminate both GC tissues and adjacent-normal tissues from non-GC gastritis, intestinal metaplasia and normal gastric tissues. RESULTS: A signature consisting of two gene pairs for GC diagnosis was identified and validated in data of both biopsy specimens and surgical resection specimens pooled from publicly available datasets measured by different laboratories with different platforms. For gastroscopy biopsy specimens, 96.20% of 79 non-GC tissues were correctly identified as non-GC, and 96.84% of 158 GC tissues and six of seven adjacent-normal tissues were correctly identified as GC. For surgical resection specimens, 98.37% of 2560 GC tissues and 97.28% of 221 adjacent-normal tissues were correctly identified as GC. Especially, 97.67% of the 257 GC patients at stage I were exactly diagnosed as GC. We additionally measured 21 GC tissues from seven different GC patients, each with three specimens sampled from three tumor locations with different proportions of the tumor epithelial cell. All these GC tissues were correctly identified as GC, even when the proportion of the tumor epithelial cell was as low as 14%. CONCLUSIONS: The qualitative transcriptional signature can distinguish both GC and adjacent-normal tissues from normal, gastritis and intestinal metaplasia tissues of non-GC patients even using inaccurately sampled biopsy specimens, which can be applied robustly at the individual level to aid the early GC diagnosis.
Assuntos
Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Transcriptoma/genética , Bases de Dados Genéticas , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Curva ROC , Reprodutibilidade dos Testes , Neoplasias Gástricas/diagnósticoRESUMO
A new starch stabilized ferromanganese binary oxide (starch-FMBO) with an Fe/Mn ratio of 1.00-2.00 and a synthetic pH of 2 was prepared using an organic polymer (starch) as the stabilizing dispersant. The maximum arsenic adsorption capacity of starch-FMBO was 161.29â¯mg/g. Adsorption optimization was also conducted, which revealed that starch-FMBO had a high adsorption capacity over a wide pH range (pH 3.0 to pH 11.0) and in the presence of some common anions (HCO3-, SO42-, Cl-, and NO3-). Arsenic removal by FMBO and starch-FMBO followed pseudo-second-order dynamics (R2â¯≥â¯0.99), indicating that the adsorption rate depended on the chemical adsorption process. Through the analysis of X-ray photoelectron spectroscopy (XPS), and Fourier-transform infrared spectroscopy (FTIR), it was shown that hydroxyl was continuously produced and complexated with As(III) and As(V) during the adsorption process. Thus, the reaction of iron oxide and manganese oxide with arsenic was inferred and explained. The developed starch-FMBO shows promise for its application in the treatment of arsenic-contaminated water.
Assuntos
Arsênio , Poluentes Químicos da Água , Purificação da Água , Adsorção , Concentração de Íons de Hidrogênio , Ferro , Manganês , Óxidos , Amido , ÁguaRESUMO
Due to the toxicity and mobility of chromium, the disposal of chromium-containing waste is a pressing issue. Co-processing of chromium-containing waste in a cement kiln is currently one of the most effective methods. However, the presence of water-soluble hexavalent chromium (Cr(VI)) in cement limits the use of this method. In this study, Na2CO3 was used to simulate alkali in industrial raw materials to investigate the pattern of influence of alkali content on water-soluble hexavalent chromium. The mechanisms associated with the oxidation and dissolution of chromium were investigated using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and inductively coupled plasma emission spectrometry (ICP-OES). The proportion of Cr(VI) in the clinker detected by XPS increased rapidly with increasing alkali content. In the cement slurry system, alkali promotes more hexavalent chromium leaching by influencing pH and other ion concentrations (Ca2+, SO42-). Therefore, the addition of alkali to either the raw meal or to the cement slurry system will favour an increase in the water-soluble Cr(VI) content. This study may provide theoretical guidance for the preparation and use of clinkers containing chromium.
Assuntos
Cromo , Materiais de Construção , Cromo/análise , Materiais de Construção/análise , Água/química , Difração de Raios XRESUMO
Chromium (Cr)-bearing electroplating sludge is a hazardous solid waste and has a detrimental effect on human health and the environment. In this study, an alkali-activated slag binders, namely, formed by the reaction of blast furnace slag (BFS) with alkali, was applied to the stabilization/solidification (S/S) of electroplating sludge. The effects of liquid-solid ratio, water glass modulus ratio (molar ratio of SiO2 to Na2O), water glass dosage, and electroplating sludge amount on the compressive strength and Cr leachability of binders were analyzed. The related mechanism of the S/S of electroplating sludge was discussed on the basis of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy coupled with energy-dispersive spectrometry (SEM-EDS). Results showed that the compressive strength of the alkali-activated slag binder first increased and then remained stable with the increase in liquid-solid ratio, water glass modulus ratio, and water glass dosage. By contrast, the leaching concentrations of Cr(VI) and total Cr decreased with the increase in liquid-solid ratio, water glass modulus ratio, water glass dosage, and curing time. In addition, XRD, FTIR, and SEM-EDS revealed that the hydration products of the binders were mainly low-crystallinity and dense calcium silicate hydrate gels, and Cr(VI) had been effectively immobilized in the structure. The reduction in Cr(VI) by the reductive components in the BFS boosted the stabilization of Cr-bearing electroplating sludge. Overall, the BFS binders containing electroplating sludge had relatively high compressive strengths and low Cr(VI) leaching concentrations. The physical encapsulation, chemical bonding, and absorption contributed the Cr immobilization during the S/S process of electroplating sludge.
Assuntos
Álcalis/química , Cromo/química , Galvanoplastia , Recuperação e Remediação Ambiental , Resíduos Perigosos/análise , Esgotos/química , HumanosRESUMO
BACKGROUND AND PURPOSE: The standard therapy for locally advanced rectal cancers (LARCs) is neoadjuvant chemoradiation (nCRT) followed by surgical resection. Pathological response to nCRT varies among patients, and it remains a challenge to predict pathological response to nCRT in LARCs. MATERIAL AND METHODS: Using 42 samples as the training cohort, we searched a signature by screening the gene pairs whose within-sample relative expression orderings are significantly correlated with the pathological response. The signature was validated in both a public cohort of 46 samples and a cohort of 33 samples measured at our laboratory. RESULTS: A signature consisting of 27 gene pairs was identified in the training cohort with an accuracy of 92.86% and an area under the receiver operating characteristic curve (AUC) of 0.95. The accuracy was 89.13% for the public test cohort and 90.91% for the private test cohort, with AUC being 0.95 and 0.91, respectively. Furthermore, the signature was used to predict disease-free survival benefits from 5Fu-based chemotherapy in 285 locally advanced colorectal cancers. CONCLUSIONS: The signature consisting of 27 gene pairs can robustly predict clinical response of LARCs to nCRT.
Assuntos
Quimiorradioterapia/métodos , Neoplasias Retais/terapia , Idoso , Quimiorradioterapia/mortalidade , Intervalo Livre de Doença , Feminino , Perfilação da Expressão Gênica/métodos , Genes Neoplásicos/genética , Humanos , Masculino , Pessoa de Meia-Idade , Terapia Neoadjuvante/métodos , Terapia Neoadjuvante/mortalidade , Neoplasias Retais/genética , Neoplasias Retais/mortalidade , Resultado do TratamentoRESUMO
A bacterial strain HS-B1 which can utilize n-butyl benzyl phthalate as the sole source of carbon and energy was isolated from contaminated sludge in the Jinsha River of Hubei Province. Based on its morphology, physiological characteristic and 16S rDNA sequence analysis, HS-B1 was identified as Acinetobacter sp.. Shake flask experiments indicated the optimum pH, temperature for growth and BBP biodegradation by HS-B1 was found to be 8.0 and 35 degrees C, respectively. When using 0.10 mmol x L(-1) non-ionic surfactant Tween-80 as co-solvent to increase the apparent solubility of BBP in water, HS-B1 was able to completely degrade 1 000 mg x L(-1) BBP within 48 hours. Diversity of degradable substrates also showed that HS-B1 can efficiently utilize many other phthalate esters such as dimethyl phthalate and diethyl phthalate. It revealed that the strain HS-B1 has special application potential in dealing with the pollution caused by phthalate esters.