[Exploration and practice of micro-video teaching of Characteristic Clinical Technology of Acupuncture and Moxibustion].

In this paper, the micro-video teaching mode was explored in the course construction of Characteristic Clinical Technology of Acupuncture and Moxibustion. The micro-video teaching contents include the academic thought, experience in diagnosis and treatment, characteristic technology and clinical manipulation of famous acupuncture experts in the Henan University of CM. Each micro-video film is designed within 15-18 min, including three sections of knowledge, i.e. basic theory, technological application and clinical manipulation. Each section is designed within 5-6 min. The construction of the teaching course of Characteristic Clinical Technology of Acupuncture and Moxibustion is the innovation of practice mode of TCM and the new approach to the inheritance of the experience of experts. The construction of micro-video teaching course propels the reform of teaching mode, improves the learning initiative of students and clinical manipulative ability so as to improve the teaching effect and quality.

[Discussion on WANG Guo-rui's acupuncture academy thought].

Through study on Bianque Shenying Zhenjiu Yulong Jing (Bianque's Classic of Effective Acupuncture Prescriptions), acupuncture experiences and ideology of WANG Guo-rui, an acupuncture physician of the Yuan Dynasty (1271-1368), is discussed in this article. Inherited and carried forward DOU's acupuncture theory, WANG Guo-rui encouraged to use experiential points and to expand extra points. Penetrating needling method was created, and the application of acupoints according to needling opportunities and seasonal factors were enriched by him. Great importance was also attached on treatment according to differentiation of syndromes, which including the simultaneous acupuncture and moxibustion, simultaneous reinforcing and reducing, reducing after reinforcing, reinforcing after reducing, predomination of reducing and predomination of reinforcing. The flexible treating methods are still extensively adopted in clinic nowadays. WANG Guo-rui is a master of clinical acupuncture who made great contribution to the development of acupuncture and moxibustion.

[Effect of electroacupuncture on expression of caspase-3 and phosphorylated Akt proteins in the skeleton muscle in diabetic mice].

OBJECTIVE: To observe the effect of electroacupuncture(EA) on expression of phosphorylated (pAkt) and caspase-3 proteins in the skeletal muscle in mice with diabetes, so as to provide experimental evidence for treating diabetic myopathy in clinic. METHODS: Eighteen C 57 mice were equally randomized into control, model and EA groups (n = 6 mice/ group). Type 1 diabetic model was established by streptozotocin (STZ, i. P., 200 mg/kg). EA (1 mA, 20-30 Hz) was applied to bilateral "Zusanli"(ST 36) and "Yanglingquan" (GB 34) for 20 min, once daily for 14 days. Damp weights of the musculus soleus, plantaris, gastrocnemius, extensor digitorum longus and tibialis anterior were measured, and Western blot was used to measure the expression levels of pAkt (ser 473), pAkt(Thr 308), total Akt and caspase-3 proteins in the aforementioned skeleton muscles. RESULTS: Compared with the normal control group, the weights of the musculus soleus, plantaris, gastrocnemius, extensor digitorum longus and tibialis anterior were decreased obviously in the model group (P < 0.01). After EA treatment, the weights of the 5 skeleton muscles were all increased apparently compared with the model group (P < 0.05). In comparison with the normal control group, muscular pAkt (Thr 308) protein expression level was down-regulated significantly in the model group (P < 0.01), while that of muscular caspase-3 protein expression of the model group was up-regulated considerably (P < 0.01). Following EA, the expression levels of muscular pAkt (Thr 308) and pAkt (ser 473) in the EA group were upregulated apparently (P < 0.01), while that of muscular caspase-3 protein was down-regulated obviously (P < 0.01). No significant differences were found between the control and model groups in the expression levels of muscular pAkt (ser 473) and total Akt proteins (P > 0.05). CONCLUSION: EA intervention can effectively up-regulate expression of pAkt (Thr 308 and ser 473) and down-regulate expression of caspase-3 protein in the skeleton muscle in diabetic mice, which may contribute to its effect in inhibiting diabetic myopathy.