Oxidative stress enhances AP-1 and NF-κB-mediated regulation of ß(2)-glycoprotein I gene expression in hepatoma cells.

ß(2)-Glycoprotein I (ß(2)-GPI), also known as apolipoprotein H, is a plasma glycoprotein with poorly defined gene regulation. The aim of this study was to clarify the role of oxidative stress in ß(2)-GPI gene regulation and determine the essential transcription element regulating ß(2)-GPI expression. We demonstrate that expression of ß(2)-GPI at the protein and mRNA levels was significantly elevated in Huh7 and HepG2 cells treated with 100 µM hydrogen peroxide (H(2)O(2)). To address the transcriptional mechanism of H(2)O(2)-mediated ß(2)-GPI gene regulation, several promoter constructs were cloned and characterized by deletion assays. A region spanning from -2141 to -1419 (relative to the transcription start site), which contains two activator protein-1 (AP-1) sites (AP1-2 and AP1-3) and one nuclear factor-kappaB (NF-κB) site was found to be the main target site for up-regulation of ß(2)-GPI promoter activity by oxidative stress. In addition, we found that H(2)O(2) stimulation enhanced the nuclear translocation of AP-1 and NF-κB subunits. Using an electrophoretic mobility shift assay, it was confirmed that nuclear protein binding to the AP1-2, AP1-3, and NF-κB sites was increased in Huh7 cells treated with H(2)O(2). Knockdown of the c-Jun, c-Fos, p65, and p50 genes using small interfering RNAs (siRNAs) further confirmed that AP-1 and NF-κB play an essential role in the H(2)O(2)-induced ß(2)-GPI expression. Overall, these findings provide new insight suggesting that multiple cis-elements in the ß(2)-GPI promoter work cooperatively to regulate ß(2)-GPI expression in cells under oxidative stress.