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1.
Rev Sci Instrum ; 94(8)2023 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38065132

RESUMO

This work considers a solenoid-based magnetic collimation system for improving the efficiency of ion trap loading with ions created by laser ablation. We discuss a physical model of ion beam collimation in such a system, provide qualitative analytical estimates of its collimation characteristics, develop a numerical model of ion collimation based on a test-particle approach, and describe a real experimental setup where the proposed approach is effectively employed to collimate 232Th3+ and 88Sr1+ ions. The experimental results are compared with the results of the performed numerical modeling. The observed inconsistencies between the two are discussed, and their possible explanations are suggested.

2.
Mol Gen Mikrobiol Virusol ; (1): 21-4, 1991 Jan.
Artigo em Russo | MEDLINE | ID: mdl-1827173

RESUMO

The pesticinogenicity 9.5 kb plasmid from Yersinia pestis strain EV76 has been marked by the kanamycin phosphotransferase gene inserted into PstI site and designated pP3. The obtained plasmid pP3 determines the synthesis of 45 kd pesticin, alpha and beta-forms of fibrinolysin coagulase (37 and 35 kd) and the 29, 19 and 13 kd proteins in Escherichia coli mini cells. When transferred into Yersinia pseudotuberculosis strain 6933 the plasmid causes the proteolysis of outer membrane proteins. The 150 kd protein is reduced to 138 kd, the 48.5 kd protein is reduced to 45 kd. The proteins secreted into the cultural medium (51 and 38 kd) are also cleaved. The proteolysis of the 150 kd protein was found to occur at the stage of secretion via the inner membrane. The purified fibrinolysin coagulase from Escherichia coli strain JM83 harbouring the plasmid pP3 induces the proteolysis in vitro of the isolated membrane proteins from Yersinia pseudotuberculosis strain 6953 similar to the proteolysis registered in vivo.


Assuntos
Proteínas da Membrana Bacteriana Externa/biossíntese , Cálcio/metabolismo , Coagulase/metabolismo , Fibrinolisina/metabolismo , Yersinia pestis/enzimologia , Yersinia pseudotuberculosis/enzimologia , Autorradiografia , Proteínas da Membrana Bacteriana Externa/genética , Eletroforese em Gel de Poliacrilamida , Genes Bacterianos , Hidrólise , Mutação , Plasmídeos , Yersinia pestis/patogenicidade , Yersinia pseudotuberculosis/genética
3.
Mol Gen Mikrobiol Virusol ; (12): 19-26, 1991 Dec.
Artigo em Russo | MEDLINE | ID: mdl-1787840

RESUMO

The genetical libraries of the pFra plasmid of Yersinia pestis genes were obtained by insertion into the PstI, SalGI, EcoRI, XhoI restriction sites of the cosmid vector pHC79. The immunochemical analysis of the recombinant clones has revealed the clones synthesizing the antigen Fl (fraction I) and mouse toxin (Ymt--Yersinia pestis murine toxin). The restriction analysis of the plasmids from antigen synthesizing clones has permitted to construct the detailed physical map of the fra-ymt region of the pFra plasmid the size of 22 kb. The recombinant F1 positive clones of Escherichia coli are able to form at 37 degrees C the capsule-like structure peculiar for Yersinia pestis. The antigen F1 and the mouse toxin were isolated, purified and characterized. The antigen F1 is an 1-2 Md polymer containing a 16 kDa protein subunit. The mouse toxin a 240 kDa protein consisting of 61 kDa subunits. The nucleotide sequence of ymt gene has been defined.


Assuntos
Plasmídeos , Yersinia pestis/genética , Sequência de Aminoácidos , Antígenos de Bactérias/genética , Sequência de Bases , Clonagem Molecular , DNA Bacteriano/genética , Eletroforese em Gel de Poliacrilamida , Escherichia coli/metabolismo , Genes Bacterianos , Imuno-Histoquímica , Dados de Sequência Molecular , Fenótipo , Mapeamento por Restrição
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