Ideal Combinations of Acceleration-Based Intensity Metrics and Sensor Positions to Monitor Exercise Intensity under Different Types of Sports.

This study quantified the strength of the relationship between the percentage of heart rate reserve (%HRR) and two acceleration-based intensity metrics (AIMs) at three sensor-positions during three sport types (running, basketball, and badminton) under three intensity conditions (locomotion speeds). Fourteen participants (age: 24.9 ± 2.4 years) wore a chest strap HR monitor and placed three accelerometers at the left wrist (non-dominant), trunk, and right shank, respectively. The %HRR and two different AIMs (Player Load per minute [PL/min] and mean amplitude deviation [MAD]) during exercise were calculated. During running, both AIMs at the shank and PL at the wrist had strong correlations (r = 0.777-0.778) with %HRR; while other combinations were negligible to moderate (r = 0.065-0.451). For basketball, both AIMs at the shank had stronger correlations (r = 0.604-0.628) with %HRR than at wrist (r = 0.536-0.603) and trunk (r = 0.403-0.463) with %HRR. During badminton exercise, both AIMs at shank had stronger correlations (r = 0.782-0.793) with %HRR than those at wrist (r = 0.587-0.621) and MAD at trunk (r = 0.608) and trunk (r = 0.314). Wearing the sensor on the shank is an ideal position for both AIMs to monitor external intensity in running, basketball, and badminton, while the wrist and using PL-derived AIM seems to be the second ideal combination.

PACAP38/PAC1 signaling induces bone marrow-derived cells homing to ischemic brain.

Understanding stem cell homing, which is governed by environmental signals from the surrounding niche, is important for developing effective stem cell-based repair strategies. The molecular mechanism by which the brain under ischemic stress recruits bone marrow-derived cells (BMDCs) to the vascular niche remains poorly characterized. Here we report that hypoxia-inducible factor-1α (HIF-1α) activation upregulates pituitary adenylate cyclase-activating peptide 38 (PACAP38), which in turn activates PACAP type 1 receptor (PAC1) under hypoxia in vitro and cerebral ischemia in vivo. BMDCs homing to endothelial cells in the ischemic brain are mediated by HIF-1α activation of the PACAP38-PAC1 signaling cascade followed by upregulation of cellular prion protein and α6-integrin to enhance the ability of BMDCs to bind laminin in the vascular niche. Exogenous PACAP38 confers a similar effect in facilitating BMDCs homing into the ischemic brain, resulting in reduction of ischemic brain injury. These findings suggest a novel HIF-1α-activated PACAP38-PAC1 signaling process in initiating BMDCs homing into the ischemic brain for reducing brain injury and enhancing functional recovery after ischemic stroke.

Dissolved Organic Matter or Salts Change the Bioavailability Processes and Toxicity of the Nanoscale Tetravalent Lead Corrosion Product PbO2 to Medaka Fish.

Nanoscale lead dioxide (nPbO2(s)) is a corrosion product formed from the chlorination of lead-containing plumbing materials. This metal oxide nanoparticle (NP) plays a key role in determining lead pollution in drinking water and receiving water bodies. This study uses nPbO2(s) and medaka fish (Oryzias latipes) as surrogates to investigate the aqueous fate and toxicological risk of metal oxide NPs associated with water matrices. The larvae of medaka were treated with solutions containing nPbO2(s) or Pb(II)aq in different water matrices for 7-14 days to investigate the in vivo toxic effects of NPs. Ionic strength enhanced aggregation and sedimentation of nPbO2(s) in water, leading to increased lead contents in fish bodies. However, the presence of dissolved organic matter in water enhanced particle stability and accelerated the lead dissolution, thus changing the bioavailability processes (bioaccessibility) of particles. Oxidative stress response and neurotoxicity in exposed fish was greater for nPbO2(s) solution with increased salinity than dissolved organic matter. We predict the bioavailability processes and toxicity of nPbO2(s) in medaka from the aqueous particle behavior under environmentally relevant exposure conditions. Our investigation suggests a toxicological risk of metal oxide NP pollution in the aquatic environment.

Multitheragnostic Multi-GNRs Crystal-Seeded Magnetic Nanoseaurchin for Enhanced In Vivo Mesenchymal-Stem-Cell Homing, Multimodal Imaging, and Stroke Therapy.

A multifunctional nanoseaurchin probe in which mesoporous silica nanobeads with iron oxide nanoparticles embedded and multi-gold nanorods crystal-seeded are fabricated and labeled with umbilical cord mesenchymal stem cells through endocytosis. This nanoplatform enables efficient magnetic remote-controlled guiding for stem cell homing, and provides dual modalities of photoacoustic imaging and magnetic resonance imaging for in situ tracking and long-term monitoring to achieve therapeutic efficacy.